FastQCFastQC Report
Thu 26 May 2016
SRR937651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009443
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58770.5822022640208511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.4980964749867006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36060.3572267081945192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11260.11154666484387925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.11055601950778797No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT11060.10956537417169668No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.10718782536507758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3000.034.8315359
GGTATCA36050.033.886121
GTATCAA54250.030.3158931
TCAACGC65600.024.7059464
CGTCCTA1351.34305465E-524.6283599
CAACGCA66300.024.5167875
ATCAACG66900.024.1548163
AACGCAG67450.024.1536836
TATCAAC67400.024.116662
GTACATG37050.023.3494781
CGGAGGT2252.8139766E-923.2210249
TAGGACG2303.59978E-922.7308564
TACATGG37900.022.4473322
GAGTACT47600.022.4016812-13
GGACGTG2154.422691E-822.0918836
GTATAGA3450.022.0442261
TACACGG2858.367351E-1121.679515
CGCAGAG74900.021.6243868
ACGCAGA75050.021.5811657
ACATGGG38450.021.5081883
Produced by FastQC (version 0.11.5)