FastQCFastQC Report
Thu 26 May 2016
SRR937650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045621
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51450.4920520915322091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39940.38197396571032904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.2670183555992085No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13690.1309269802347122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12980.12413675700851455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.037.41931
GTATCAA50800.032.5740431
CATTCCG2850.028.3349769
TCAACGC58800.027.5708334
CAACGCA59400.027.288415
ATCAACG59900.026.983863
AACGCAG60600.026.8868736
TATCAAC62200.026.2930452
ATAGCCC4700.025.2868143
TAGGACG2101.2514647E-924.9026874
GTACATG38600.024.3912471
CGCAGAG67950.023.7688568
TCGAATA1000.001652985623.7513799
ACGCAGA68150.023.69917
GAGTACT39500.023.62715512-13
TACATGG39300.023.2263772
GCAGAGT71900.021.8684449
ACATGGG40250.021.377883
GTACTTT43250.021.3019814-15
AGTACTT40750.020.27998412-13
Produced by FastQC (version 0.11.5)