##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937650_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1045621 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.690369646363262 33.0 31.0 34.0 30.0 34.0 2 31.820195845339754 33.0 31.0 34.0 30.0 34.0 3 31.872147747606448 33.0 31.0 34.0 30.0 34.0 4 35.463508288376 37.0 35.0 37.0 33.0 37.0 5 35.46084671214522 37.0 35.0 37.0 33.0 37.0 6 35.55816304377973 37.0 35.0 37.0 33.0 37.0 7 35.50789339540809 37.0 35.0 37.0 33.0 37.0 8 35.496069799669286 37.0 35.0 37.0 33.0 37.0 9 37.11299218359233 39.0 37.0 39.0 33.0 39.0 10-11 37.01962470149318 39.0 37.0 39.0 33.0 39.0 12-13 36.92807240864519 39.0 37.0 39.0 33.0 39.0 14-15 38.07912953163718 40.0 38.0 41.0 33.0 41.0 16-17 38.02083833434868 40.0 38.0 41.0 33.0 41.0 18-19 38.01841393774609 40.0 38.0 41.0 33.0 41.0 20-21 37.88531886792633 40.0 38.0 41.0 33.0 41.0 22-23 37.74823621560776 40.0 37.0 41.0 32.5 41.0 24-25 37.63208992550838 40.0 37.0 41.0 32.0 41.0 26-27 37.73449509908466 40.0 37.0 41.0 33.0 41.0 28-29 37.69203325105368 40.0 37.0 41.0 33.0 41.0 30-31 37.51068312514764 40.0 37.0 41.0 32.0 41.0 32-33 37.30082601630993 39.5 36.5 41.0 32.0 41.0 34-35 37.079690920515176 39.0 36.0 41.0 31.0 41.0 36-37 37.06247005368102 39.0 36.0 41.0 31.0 41.0 38-39 37.02239339110443 39.5 36.0 41.0 31.0 41.0 40-41 36.88098268875625 39.5 36.0 41.0 31.0 41.0 42-43 36.66570822506434 39.0 36.0 41.0 30.0 41.0 44-45 36.439145254351246 39.0 36.0 41.0 30.0 41.0 46-47 36.102848450824915 39.0 35.5 41.0 29.5 41.0 48-49 35.817363078974125 39.0 35.0 41.0 28.5 41.0 50-51 34.95586163629078 38.0 34.0 39.5 26.5 40.5 52-53 35.08840918458983 38.5 35.0 40.0 27.0 40.5 54-55 35.45572726638046 39.0 35.0 40.0 27.0 41.0 56-57 35.24294175423026 39.0 35.0 40.0 26.5 41.0 58-59 35.07451935261438 39.0 35.0 40.0 26.0 41.0 60-61 34.834121541170276 38.0 34.5 40.0 26.0 41.0 62-63 34.52140067959614 38.0 34.0 40.0 25.0 41.0 64-65 34.21698493048629 38.0 34.0 40.0 23.5 41.0 66-67 33.83762902619591 37.5 33.5 40.0 22.5 41.0 68-69 33.37944771575934 37.0 33.0 39.0 22.0 41.0 70-71 32.976189747528025 36.0 32.0 39.0 20.5 40.5 72-73 32.51098246879127 36.0 32.0 39.0 19.0 40.0 74-75 32.01138318759857 35.0 31.0 38.0 18.0 40.0 76-77 31.532989008445696 35.0 31.0 37.0 14.5 39.0 78-79 30.992528363527512 35.0 31.0 37.0 9.5 39.0 80-81 30.45690646993509 34.5 30.0 36.0 7.0 38.5 82-83 29.921704422539335 34.0 29.5 36.0 2.0 37.0 84-85 29.421922474778146 34.0 29.0 35.0 2.0 37.0 86-87 28.904711649823405 34.0 29.0 35.0 2.0 36.5 88-89 28.404440997263826 34.0 28.5 35.0 2.0 36.0 90-91 27.89250168081934 33.5 27.0 35.0 2.0 36.0 92-93 27.373932333034627 33.0 26.0 35.0 2.0 35.0 94-95 26.749906514884458 33.0 25.5 35.0 2.0 35.0 96-97 25.984242378452613 32.0 24.5 34.5 2.0 35.0 98-99 25.06237106944103 32.0 19.0 34.0 2.0 35.0 100-101 22.457741858665806 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1635415674262184 1101 2 0.3927044553103265 1101 3 0.23065755599932558 1101 4 0.07731601677676991 1101 5 0.2188986940792148 1101 6 0.158639772088236 1101 7 0.11745821645649102 1101 8 0.20225896691056988 1101 9 0.3947018483041873 1101 10-11 0.21312627320936883 1101 12-13 0.2361967268324392 1101 14-15 0.34885288600075626 1101 16-17 0.48852419115122814 1101 18-19 0.3826031832455996 1101 20-21 0.5468808555580225 1101 22-23 0.5371652959490874 1101 24-25 0.3268864168765262 1101 26-27 0.47135516407143285 1101 28-29 0.5029907125444026 1101 30-31 0.4879735235475309 1101 32-33 0.5153482878500739 1101 34-35 0.6183336611206656 1101 36-37 0.4905731233746451 1101 38-39 0.7793548999092579 1101 40-41 0.6738229096446702 1101 42-43 0.64619136035639 1101 44-45 0.8978713848271411 1101 46-47 1.0113844754367491 1101 48-49 1.3066729554141574 1101 50-51 1.1748588818962347 1101 52-53 0.9507197485271419 1101 54-55 1.166455615070646 1101 56-57 1.2167107461112963 1101 58-59 1.3739644437410021 1101 60-61 1.314797089509561 1101 62-63 1.32535406227219 1101 64-65 1.3044577494880656 1101 66-67 1.5298120235145163 1101 68-69 1.5967774387635174 1101 70-71 1.6906960575160355 1101 72-73 1.3922337144479044 1101 74-75 1.4636648793523293 1101 76-77 1.5153672620162695 1101 78-79 1.4267275253854415 1101 80-81 1.648984532457245 1101 82-83 1.546893335685759 1101 84-85 1.832385082056522 1101 86-87 1.8898840362828615 1101 88-89 1.9559198916934406 1101 90-91 2.2022622634318445 1101 92-93 2.0162130336566655 1101 94-95 2.291378446762831 1101 96-97 2.2383301067909187 1101 98-99 2.5408739420301067 1101 100-101 3.1609065398381233 1102 1 0.2058123462282353 1102 2 0.1804704732914395 1102 3 0.21562777715936576 1102 4 -0.06336149925068923 1102 5 0.07993962575859115 1102 6 0.1396723581054573 1102 7 0.195042526611644 1102 8 0.14403279946624536 1102 9 0.09748247119406273 1102 10-11 0.22959947225341182 1102 12-13 0.36714206274534433 1102 14-15 0.5027886244021786 1102 16-17 0.46726741030072816 1102 18-19 0.5684193510692808 1102 20-21 0.4646457354942939 1102 22-23 0.39330694459670923 1102 24-25 0.4949414982467246 1102 26-27 0.4886249945027501 1102 28-29 0.5717532784561712 1102 30-31 0.6235070769028681 1102 32-33 0.425048223360875 1102 34-35 0.5260856432154242 1102 36-37 0.5609194683539727 1102 38-39 0.411237226072906 1102 40-41 0.4073781617216028 1102 42-43 0.6926577311313977 1102 44-45 0.5341351836208119 1102 46-47 0.7946628032695244 1102 48-49 0.8581315495494337 1102 50-51 0.7231404341893963 1102 52-53 0.9414389928653648 1102 54-55 0.9224853559757733 1102 56-57 1.1758587298751877 1102 58-59 1.2086292895359279 1102 60-61 1.1723897453268606 1102 62-63 1.1453267491960517 1102 64-65 1.1330264684289233 1102 66-67 1.0207114582855752 1102 68-69 1.0018455317797077 1102 70-71 1.0032486920898336 1102 72-73 1.0115482321065699 1102 74-75 0.9298308062898499 1102 76-77 0.9942546335120426 1102 78-79 1.1998753574100256 1102 80-81 1.2726729357803386 1102 82-83 1.349505148591188 1102 84-85 1.4295494536660982 1102 86-87 1.4904454717367521 1102 88-89 1.770851039980684 1102 90-91 1.8944856236989054 1102 92-93 1.9502898896181229 1102 94-95 2.0999536146244466 1102 96-97 2.0377497039230477 1102 98-99 2.3115389395643433 1102 100-101 2.1894221500198334 1103 1 0.13863488893266762 1103 2 0.30651973416607703 1103 3 0.27617627573912884 1103 4 0.09624642295631958 1103 5 0.07687629691766773 1103 6 0.25979658917808024 1103 7 0.23906815103903511 1103 8 0.3028872701223122 1103 9 0.2697434271932053 1103 10-11 0.38259258506951 1103 12-13 0.24717170558101031 1103 14-15 0.4556200811813014 1103 16-17 0.3935228975857896 1103 18-19 0.3992199552454494 1103 20-21 0.42879311486245086 1103 22-23 0.3946051451563193 1103 24-25 0.37536555351399414 1103 26-27 0.1229887524219535 1103 28-29 0.42343828618523816 1103 30-31 0.42838081186479116 1103 32-33 0.4762900034874349 1103 34-35 0.4942160575430279 1103 36-37 0.6099433418024063 1103 38-39 0.6712257651197362 1103 40-41 0.6776019686682133 1103 42-43 0.7306168325077707 1103 44-45 0.45505991274966817 1103 46-47 0.7557574657904453 1103 48-49 0.9215046071124249 1103 50-51 0.6875265834152486 1103 52-53 0.6792909131056248 1103 54-55 0.749591217687346 1103 56-57 0.9731767253403305 1103 58-59 0.8401543391469986 1103 60-61 0.7095193425309603 1103 62-63 0.5780095673170962 1103 64-65 0.8379352229699677 1103 66-67 0.913213731020889 1103 68-69 0.9982816705704423 1103 70-71 0.9171217514214973 1103 72-73 0.9113692431465807 1103 74-75 0.8725313464403328 1103 76-77 0.7690964263729967 1103 78-79 0.7310905368057767 1103 80-81 0.9688038028863417 1103 82-83 1.006498983022862 1103 84-85 1.3636135996305327 1103 86-87 1.2000181974980748 1103 88-89 1.1084238647872802 1103 90-91 1.2993314137441487 1103 92-93 1.3905302159015562 1103 94-95 1.5413488795528671 1103 96-97 1.7207376168622517 1103 98-99 1.8924465480100743 1103 100-101 2.2867482813467923 1104 1 0.08075031321105186 1104 2 0.24809079999287675 1104 3 0.26779450802804305 1104 4 0.14941435246696955 1104 5 0.09623652358717294 1104 6 0.09170478532293913 1104 7 0.28482469094794993 1104 8 0.08553609806108398 1104 9 0.2570608896037285 1104 10-11 -0.015879492046799726 1104 12-13 0.1641908527978444 1104 14-15 0.2373948925999798 1104 16-17 0.2138169863939794 1104 18-19 0.3301067185441937 1104 20-21 0.32830150170182293 1104 22-23 0.2874916312041691 1104 24-25 0.25757280869638777 1104 26-27 0.41266014249703886 1104 28-29 0.31198850071656636 1104 30-31 0.4772479714970572 1104 32-33 0.41628378224799434 1104 34-35 0.503835310926668 1104 36-37 0.5691147673791406 1104 38-39 0.5746139738423253 1104 40-41 0.6326230836901416 1104 42-43 0.6171874080738533 1104 44-45 0.5252128402823715 1104 46-47 0.2742656531444183 1104 48-49 0.4297376060541396 1104 50-51 0.36651739018152085 1104 52-53 0.6199118874734424 1104 54-55 0.5775401359017138 1104 56-57 0.6081099991661532 1104 58-59 0.7630430905374794 1104 60-61 0.842735013292149 1104 62-63 0.8385053730521292 1104 64-65 0.8309126608336541 1104 66-67 0.5647262698613744 1104 68-69 0.927246273746654 1104 70-71 0.8900796464786467 1104 72-73 0.9196005515340957 1104 74-75 0.8442044203073991 1104 76-77 0.7580537263550795 1104 78-79 0.8116961609414801 1104 80-81 0.8523077131381989 1104 82-83 0.9791914610710144 1104 84-85 1.1496790376024073 1104 86-87 1.3134526935945843 1104 88-89 1.2979772541793935 1104 90-91 1.0003078778486092 1104 92-93 1.2304448767256666 1104 94-95 1.3288580508093908 1104 96-97 1.5047828425681402 1104 98-99 1.7705051831918617 1104 100-101 1.7755517408969013 1105 1 0.11846564859551023 1105 2 0.24010051036922064 1105 3 0.12449181171097123 1105 4 0.20158166173207093 1105 5 0.3620872849689505 1105 6 0.21845245833770122 1105 7 0.37876089844882443 1105 8 0.3801999346982825 1105 9 0.19639051014610942 1105 10-11 0.23379109511940044 1105 12-13 0.33935409876375644 1105 14-15 0.3314057453735373 1105 16-17 0.42889598056515865 1105 18-19 0.4797331676361196 1105 20-21 0.6441775775673833 1105 22-23 0.6269020633678508 1105 24-25 0.6576406385311273 1105 26-27 0.736964643282441 1105 28-29 0.6205655670924415 1105 30-31 0.5575208486504124 1105 32-33 0.5081218084971653 1105 34-35 0.45305056192951554 1105 36-37 0.5161052647113848 1105 38-39 0.7937546444814174 1105 40-41 0.8768348296106154 1105 42-43 0.8025770563972543 1105 44-45 0.6403823824147636 1105 46-47 0.7999647223503246 1105 48-49 0.6018517549033078 1105 50-51 0.5824791458383416 1105 52-53 0.5724488531106502 1105 54-55 0.6976249069679099 1105 56-57 0.8053406595496355 1105 58-59 0.979073000573699 1105 60-61 0.8033028366359929 1105 62-63 0.8254392048860666 1105 64-65 0.7945847538871007 1105 66-67 0.932529491940798 1105 68-69 0.9901008387918964 1105 70-71 1.2085618159948197 1105 72-73 1.2130262502787588 1105 74-75 1.3177978437343327 1105 76-77 1.1975050511463934 1105 78-79 1.1523057856380312 1105 80-81 1.0448941393821798 1105 82-83 1.2155005444270657 1105 84-85 1.1877951856467028 1105 86-87 1.0753423873542367 1105 88-89 1.2455892545428782 1105 90-91 1.3764356705554448 1105 92-93 1.6225029442569188 1105 94-95 1.6305937156715906 1105 96-97 1.6521764243728683 1105 98-99 1.9308448516370298 1105 100-101 1.985321184367713 1106 1 0.20272717966741638 1106 2 0.25439994825785917 1106 3 0.26043383505222195 1106 4 0.21355735982764656 1106 5 0.1866676487186112 1106 6 0.21157860235764048 1106 7 0.16600239862408017 1106 8 0.3242322074196551 1106 9 0.28545205679616004 1106 10-11 0.07875773192795776 1106 12-13 -0.05768086118743554 1106 14-15 0.1869217064801063 1106 16-17 0.12233433655170955 1106 18-19 0.19130545671454513 1106 20-21 0.27677679087111784 1106 22-23 0.05720771953225068 1106 24-25 -0.0421117338903656 1106 26-27 0.0693373244108173 1106 28-29 0.4045226752696607 1106 30-31 0.41731106297971365 1106 32-33 0.5256423521743869 1106 34-35 0.3103547641338267 1106 36-37 0.25155430470826445 1106 38-39 0.32119651853212616 1106 40-41 0.16259153794882053 1106 42-43 0.3779655363598664 1106 44-45 0.21080695174399722 1106 46-47 0.22116365524747295 1106 48-49 0.44025285316244833 1106 50-51 0.3255079264591316 1106 52-53 0.4159160935722852 1106 54-55 0.518128989739779 1106 56-57 0.45774196551736424 1106 58-59 0.38554571934925974 1106 60-61 0.28279810477479117 1106 62-63 0.5277798210016584 1106 64-65 0.5648769089934831 1106 66-67 0.885967069020154 1106 68-69 0.8383924356814632 1106 70-71 0.7428661869623525 1106 72-73 0.5778550205141144 1106 74-75 0.3909972699393691 1106 76-77 0.719673074409755 1106 78-79 0.6066488423294949 1106 80-81 0.44904798653462663 1106 82-83 0.5643649626483054 1106 84-85 0.4330649471712853 1106 86-87 0.5447881299773378 1106 88-89 0.7217417126441639 1106 90-91 0.8357442606036081 1106 92-93 0.7204553919727736 1106 94-95 0.611718198022853 1106 96-97 0.6661918856806537 1106 98-99 0.8866986637386525 1106 100-101 0.8454781341671413 1107 1 0.48629549658408777 1107 2 0.5431310143186856 1107 3 0.526799388412865 1107 4 0.5593610307896384 1107 5 0.40455124911211726 1107 6 0.3840184898185228 1107 7 0.46194504930215174 1107 8 0.3175311374847922 1107 9 0.6583412423558244 1107 10-11 0.5139861816010338 1107 12-13 0.5848509979782861 1107 14-15 0.7462910627233512 1107 16-17 0.7124034706307185 1107 18-19 0.8561034363413924 1107 20-21 0.7345655846220538 1107 22-23 0.6538085049568352 1107 24-25 0.5961581182975593 1107 26-27 0.6716249271490753 1107 28-29 0.731468927786942 1107 30-31 0.5894414791508282 1107 32-33 0.6557942302923863 1107 34-35 0.7355756577378187 1107 36-37 0.7118442815656891 1107 38-39 0.7393577395206066 1107 40-41 0.7266859786472253 1107 42-43 0.8416529206610832 1107 44-45 0.6009943399983158 1107 46-47 0.8388101825617014 1107 48-49 0.7518746295502439 1107 50-51 0.583191906576161 1107 52-53 0.5832734233546049 1107 54-55 0.6813993784199042 1107 56-57 0.6171292801310742 1107 58-59 0.6541933411075718 1107 60-61 0.5856157618060607 1107 62-63 0.7566428754381107 1107 64-65 0.635128021226528 1107 66-67 0.6340529213255479 1107 68-69 0.8338294283433711 1107 70-71 0.7005346935148964 1107 72-73 0.6938220448799015 1107 74-75 0.9347033128745537 1107 76-77 0.9288412178417929 1107 78-79 0.9116423987758147 1107 80-81 0.8656635452601904 1107 82-83 0.8388739028672596 1107 84-85 0.7418749397055713 1107 86-87 0.8168726901836223 1107 88-89 1.0677548979282392 1107 90-91 1.149536853848776 1107 92-93 1.1324136336788868 1107 94-95 1.3939063181654774 1107 96-97 1.4668138777947846 1107 98-99 1.7986365752140507 1107 100-101 1.8160653728813223 1108 1 0.10320285972295196 1108 2 0.259806190018967 1108 3 0.25438934934756574 1108 4 0.23275659271339322 1108 5 0.2682906002558454 1108 6 0.21281742672655213 1108 7 0.15008498148636562 1108 8 0.22672848432998194 1108 9 0.16594062388251984 1108 10-11 0.3338298372066575 1108 12-13 0.3415850055875467 1108 14-15 0.373592790975664 1108 16-17 0.3292531248450814 1108 18-19 0.308778478947211 1108 20-21 0.26983041659550366 1108 22-23 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sequence quality scores pass #Quality Count 2 3525.0 3 515.0 4 440.0 5 541.0 6 638.0 7 715.0 8 885.0 9 1181.0 10 1544.0 11 2167.0 12 3097.0 13 4209.0 14 5062.0 15 6427.0 16 8513.0 17 11251.0 18 9257.0 19 6258.0 20 6003.0 21 6262.0 22 6953.0 23 7684.0 24 8958.0 25 10280.0 26 11996.0 27 14218.0 28 17042.0 29 20554.0 30 25314.0 31 31442.0 32 39745.0 33 51611.0 34 69113.0 35 95846.0 36 138536.0 37 207680.0 38 186667.0 39 23488.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.24155660001746 22.108309996037697 15.348505213834867 22.301628190109977 2 14.066586175845275 21.93362096495005 42.11843658566758 21.881356273537094 3 17.285171139121555 26.3781026307336 32.26640073259722 24.07032549754762 4 11.113093011881816 18.81498316982652 41.00175494116632 29.070168877125347 5 11.52414289139295 39.37645648525533 36.70783984655957 12.391560776792137 6 27.830359555859673 40.311120566886515 19.124273806032658 12.734246071221156 7 25.608235725901274 34.963121112432454 22.802814620464165 16.625828541202107 8 23.714299951320296 37.17337641679028 21.493719551226768 17.618604080662656 9 24.967869988700585 17.500002395973997 23.208266685323316 34.3238609300021 10-11 22.44368904218422 28.459519266431354 30.102918499933395 18.993873191451026 12-13 23.083417464898332 27.037209710164163 29.746685328141854 20.132687496795654 14-15 20.82128215006526 28.562310607149634 28.192437511140756 22.42396973164435 16-17 18.996582164590762 31.645256088528356 29.39194724632849 19.966214500552393 18-19 18.699287880261544 30.42954767484257 32.16512093770018 18.7060435071957 20-21 19.904216780315622 29.34183888068632 32.06270701372315 18.69123732527491 22-23 19.59971693769515 29.586910141133487 31.409854381979834 19.403518539191527 24-25 19.179598284206044 29.70778513000929 32.05246078832042 19.060155797464255 26-27 19.29135065470173 29.777022915758355 32.00042161545796 18.931204814081955 28-29 18.80141934030864 29.78525409881235 32.219695862493616 19.19363069838539 30-31 19.43442579354184 29.549565608227546 32.066407243903875 18.949601354326738 32-33 19.095162655273178 29.685270216781053 31.82662882893762 19.39293829900815 34-35 19.442875207055483 29.84540975303797 31.76706671439065 18.944648325515896 36-37 19.585362406670612 29.36592306568329 31.45937581592103 19.58933871172507 38-39 18.916066815449888 30.26239209504115 31.483123451272537 19.338417638236425 40-41 19.38843841369717 29.704283048294943 31.81735445031595 19.089924087691937 42-43 19.23415594171349 29.737312105719038 31.490880276561985 19.53765167600549 44-45 19.63871901192692 29.888587861322907 31.17987634713828 19.29281677961189 46-47 19.657219621287762 30.04751629390155 30.568145015244692 19.727119069566 48-49 19.50554163292492 29.90660922982563 30.833521009329495 19.75432812791996 50-51 19.410926183693856 30.046022889259966 30.54841746905254 19.994633457993633 52-53 19.556910489228294 30.513757044587404 30.47829147997322 19.451040986211083 54-55 19.90906673629593 29.981913958351797 30.27929160208792 19.829727703264354 56-57 19.991626965004738 30.396033059118032 29.985620222508135 19.626719753369095 58-59 19.846034534211594 30.081466326820465 30.28337175843584 19.7891273805321 60-61 19.996331132971047 30.155787949065015 30.18711221847672 19.66076869948722 62-63 19.817043379629716 29.930478783431397 30.565625077836557 19.686852759102326 64-65 19.80284000065142 29.861055894730487 30.28519752881816 20.050906575799928 66-67 19.68153259802783 30.11089934661686 30.05624718297075 20.151320872384563 68-69 19.71197831095613 29.873161245204027 30.245103439686925 20.169757004152917 70-71 19.727358382563352 29.88107675188693 30.606294448923165 19.78527041662655 72-73 19.98223920174121 29.573836638974893 30.422024922595874 20.02189923668802 74-75 19.623956485955375 30.051719396881698 30.13402203104505 20.190302086117875 76-77 19.552672006576127 30.00616511884021 30.29075696833275 20.150405906250914 78-79 19.403461254538303 29.912924926311696 30.199534469072947 20.484079350077053 80-81 19.453102583742048 29.822272231059994 30.19882345107924 20.52580173411872 82-83 19.5747116252673 30.098108472481975 30.179191042124142 20.14798886012658 84-85 19.652126936599227 29.75168139126237 30.0949280245287 20.5012636476097 86-87 19.4532104238977 29.84913787942429 30.16811363199873 20.52953806467928 88-89 19.997892573957483 29.515172270104966 30.13657557636905 20.350359579568504 90-91 19.618374470290526 29.350275733929333 30.5773192617142 20.454030534065943 92-93 19.817414586716097 29.414117320228566 30.553072866523934 20.215395226531406 94-95 19.75175003532401 29.258052078369012 30.509456058127753 20.480741828179223 96-97 19.977113997487233 29.078395335815443 30.122682959891062 20.821807706806265 98-99 19.74311304300339 29.063588455847167 30.156361600032188 21.03693690111725 100-101 19.906268559495757 28.72583673391191 30.808762955725427 20.559131750866907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1830.0 1 1063.5 2 321.0 3 336.5 4 291.5 5 292.0 6 482.5 7 894.0 8 1361.5 9 1486.5 10 1218.5 11 1022.5 12 1042.5 13 1082.0 14 1107.0 15 1103.5 16 1122.5 17 1251.0 18 1469.5 19 1680.5 20 1927.5 21 2413.5 22 2985.5 23 3618.5 24 4543.5 25 6263.0 26 9486.5 27 13071.0 28 14822.5 29 17129.5 30 21517.5 31 25516.5 32 30476.5 33 36441.0 34 42531.0 35 46789.0 36 49754.0 37 52452.5 38 49983.5 39 46419.5 40 45305.5 41 44124.0 42 42547.5 43 41448.0 44 40550.5 45 38693.5 46 36297.5 47 33803.5 48 32902.0 49 30720.0 50 26572.0 51 22951.5 52 19902.5 53 17503.0 54 15138.0 55 12762.5 56 10691.0 57 8844.0 58 6998.5 59 5519.0 60 4282.5 61 3214.0 62 2330.0 63 1538.5 64 945.5 65 651.0 66 448.5 67 295.0 68 223.5 69 176.5 70 126.5 71 86.0 72 67.0 73 61.0 74 50.5 75 43.5 76 32.0 77 16.0 78 16.0 79 16.5 80 11.5 81 10.0 82 6.0 83 2.0 84 2.0 85 2.0 86 2.0 87 2.0 88 1.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31541065070422264 2 0.2727565724100797 3 0.26357542551268576 4 0.2726609354632319 5 0.274573674400189 6 0.20724526381929975 7 0.1979684799750579 8 0.19749029524081863 9 0.21078383085267033 10-11 0.2079147224472347 12-13 0.20241559800348308 14-15 0.20609762045712549 16-17 0.1891698808650553 18-19 0.1957210117241333 20-21 0.18266656847940124 22-23 0.19457336836195907 24-25 0.1934257249997848 26-27 0.19280408484527378 28-29 0.20834508870805005 30-31 0.20566725419631013 32-33 0.21212274810854026 34-35 0.20279814579087452 36-37 0.18539222146456508 38-39 0.1741070617365183 40-41 0.17071195012341947 42-43 0.18639640940646754 44-45 0.18873951460424 46-47 0.18926551781190318 48-49 0.20619325740397332 50-51 0.20260687189717883 52-53 0.22527282830012021 54-55 0.1911304382754363 56-57 0.17157268264505016 58-59 0.17362887700227903 60-61 0.1627740835350476 62-63 0.1689904850801581 64-65 0.16717338309004887 66-67 0.1670299276697771 68-69 0.16985121760178878 70-71 0.1718117750121698 72-73 0.16726902003689675 74-75 0.18285784237309693 76-77 0.17649798540771466 78-79 0.1785063612915196 80-81 0.17267250753380048 82-83 0.15526658320749104 84-85 0.15488403542009962 86-87 0.1582313285597745 88-89 0.16196116948684083 90-91 0.1693252143941256 92-93 0.16506937025939608 94-95 0.16387390842379793 96-97 0.16750811240401636 98-99 0.17735871792934535 100-101 0.16076570765124265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1045621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.61945144555859 #Duplication Level Percentage of deduplicated Percentage of total 1 79.36076864107083 44.93363186755318 2 12.724867046299753 14.409499837583144 3 3.8090445355877702 6.469980364100461 4 1.4971102882171083 3.3906225308941913 5 0.7260672428515216 2.055476450142116 6 0.39018452302976075 1.3255220193895185 7 0.24063132828447617 0.9537089665658215 8 0.1623586036014628 0.7354124058705374 9 0.11741902182165101 0.5983380544334357 >10 0.7690774347608745 8.901825573872719 >50 0.11524821334466806 4.566547114234868 >100 0.081962583190281 8.557676131012899 >500 0.00407267453410177 1.5036558997005045 >1k 0.0010181686335254425 1.103769902604434 >5k 1.696947722542404E-4 0.49433288204211473 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5145 0.4920520915322091 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3994 0.38197396571032904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2792 0.2670183555992085 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1369 0.1309269802347122 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1298 0.12413675700851455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.563694684785405E-5 0.0 10-11 0.0 0.0 0.0 9.563694684785405E-5 0.0 12-13 0.0 0.0 0.0 9.563694684785405E-5 0.0 14-15 0.0 0.0 0.0 9.563694684785405E-5 0.0 16-17 0.0 0.0 0.0 9.563694684785405E-5 0.0 18-19 0.0 0.0 0.0 9.563694684785405E-5 0.0 20-21 0.0 0.0 0.0 2.3909236711963511E-4 0.0 22-23 0.0 0.0 0.0 4.7818473423927023E-4 0.0 24-25 0.0 0.0 0.0 7.650955747828324E-4 0.0 26-27 0.0 0.0 0.0 0.0011476433621742485 0.0 28-29 0.0 0.0 0.0 0.0024865606180442053 0.0 30-31 0.0 0.0 0.0 0.006312038491958367 0.0 32-33 0.0 0.0 0.0 0.012193710723101391 0.0 34-35 0.0 0.0 0.0 0.018888297002451176 0.0 36-37 0.0 0.0 0.0 0.026587071223703428 0.0 38-39 0.0 0.0 0.0 0.03395111613098818 0.0 40-41 0.0 0.0 0.0 0.03825477873914162 0.0 42-43 0.0 0.0 0.0 0.045045001965339254 0.0 44-45 0.0 0.0 0.0 0.05169176977126512 0.0 46-47 0.0 0.0 0.0 0.06096855361550696 0.0 48-49 0.0 0.0 0.0 0.07426208922735866 0.0 50-51 0.0 0.0 0.0 0.08621670758334042 0.0 52-53 0.0 0.0 0.0 0.10692210657590082 0.0 54-55 0.0 0.0 0.0 0.15383202900477325 0.0 56-57 0.0 0.0 0.0 0.23278032862767678 0.0 58-59 0.0 0.0 0.0 0.313880459554657 0.0 60-61 0.0 0.0 0.0 0.39062910940005985 0.0 62-63 0.0 0.0 0.0 0.47981056233568375 0.0 64-65 0.0 0.0 0.0 0.6033256791896873 0.0 66-67 0.0 0.0 0.0 0.7814016742203915 0.0 68-69 0.0 0.0 0.0 0.9800874312968082 0.0 70-71 0.0 0.0 0.0 1.1694007675821352 0.0 72-73 0.0 0.0 0.0 1.351923880641265 0.0 74-75 0.0 0.0 0.0 1.5699283009809482 0.0 76-77 0.0 0.0 0.0 1.8256614968521099 0.0 78-79 0.0 0.0 0.0 2.052894882562611 0.0 80-81 0.0 0.0 0.0 2.283762472253331 0.0 82-83 0.0 0.0 0.0 2.5308883429081854 0.0 84-85 0.0 0.0 0.0 2.8541890417273565 0.0 86-87 0.0 0.0 0.0 3.243001049137307 0.0 88-89 0.0 0.0 0.0 3.696893998877222 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3215 0.0 37.4193 1 GTATCAA 5080 0.0 32.574043 1 CATTCCG 285 0.0 28.334976 9 TCAACGC 5880 0.0 27.570833 4 CAACGCA 5940 0.0 27.28841 5 ATCAACG 5990 0.0 26.98386 3 AACGCAG 6060 0.0 26.886873 6 TATCAAC 6220 0.0 26.293045 2 ATAGCCC 470 0.0 25.286814 3 TAGGACG 210 1.2514647E-9 24.902687 4 GTACATG 3860 0.0 24.391247 1 CGCAGAG 6795 0.0 23.768856 8 TCGAATA 100 0.0016529856 23.751379 9 ACGCAGA 6815 0.0 23.6991 7 GAGTACT 3950 0.0 23.627155 12-13 TACATGG 3930 0.0 23.226377 2 GCAGAGT 7190 0.0 21.868444 9 ACATGGG 4025 0.0 21.37788 3 GTACTTT 4325 0.0 21.30198 14-15 AGTACTT 4075 0.0 20.279984 12-13 >>END_MODULE