FastQCFastQC Report
Thu 26 May 2016
SRR937650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045621
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59540.569422381532123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49960.47780218645187883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35240.3370246006918377No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT11770.11256468643992422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11600.11093885834351071No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.11017376276872787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.10070570503079032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.039.1339871
TAGCACG651.3260706E-436.5479744
GTATCAA50400.033.573171
GCGAGCT753.077878E-431.6673379
CCGCGAT600.003950380731.6673369
GCCGCGA650.00584323129.2313888
ATGGGCG1153.8563066E-628.920575
TCAACGC59400.028.1554764
GGCGAGC856.4059976E-427.9417678
CATTCCG2900.027.8454159
CAACGCA60250.027.837125
ATCAACG60700.027.5524753
AACGCAG61100.027.4419846
TATCAAC62650.026.8465712
ACGCAGA68900.024.2675677
GTACATG36450.023.9934751
CGCAGAG69700.023.920888
TACATGG36900.022.7904822
GAGTACT47450.022.42407812-13
GCAGAGT75100.022.1376199