##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937650_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1045621 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09238720339396 31.0 31.0 34.0 30.0 34.0 2 32.29117146652563 34.0 31.0 34.0 31.0 34.0 3 32.39213634768238 34.0 31.0 34.0 31.0 34.0 4 36.053206659009334 37.0 35.0 37.0 35.0 37.0 5 35.961553947367165 37.0 35.0 37.0 35.0 37.0 6 35.98905435143326 37.0 35.0 37.0 35.0 37.0 7 35.93872636452405 37.0 35.0 37.0 35.0 37.0 8 35.917702494498485 37.0 35.0 37.0 35.0 37.0 9 37.56544005906538 39.0 37.0 39.0 35.0 39.0 10-11 37.509741579405926 39.0 37.0 39.0 35.0 39.0 12-13 37.434640275969976 39.0 37.0 39.0 34.0 39.0 14-15 38.632210906246144 40.0 38.0 41.0 34.0 41.0 16-17 38.585954184164244 40.0 38.0 41.0 34.0 41.0 18-19 38.612597203001854 40.0 38.0 41.0 34.0 41.0 20-21 38.535718008724004 40.0 38.0 41.0 34.0 41.0 22-23 38.38201652415168 40.0 38.0 41.0 34.0 41.0 24-25 38.46420165624065 40.0 38.0 41.0 34.0 41.0 26-27 38.35552843716796 40.0 38.0 41.0 34.0 41.0 28-29 38.229751506521005 40.0 38.0 41.0 34.0 41.0 30-31 38.23767120208947 40.0 38.0 41.0 34.0 41.0 32-33 38.15265712911275 40.0 37.0 41.0 34.0 41.0 34-35 38.03184566874613 40.0 37.0 41.0 33.0 41.0 36-37 37.86532405144885 40.0 37.0 41.0 33.0 41.0 38-39 37.73693049393614 40.0 37.0 41.0 33.0 41.0 40-41 37.581622308656776 40.0 37.0 41.0 33.0 41.0 42-43 37.37602439124692 40.0 37.0 41.0 33.0 41.0 44-45 37.0643258886346 40.0 37.0 41.0 31.5 41.0 46-47 36.72068416759036 40.0 36.0 41.0 31.0 41.0 48-49 36.41757195006603 39.5 36.0 41.0 30.0 41.0 50-51 36.21249047216917 39.0 36.0 41.0 30.0 41.0 52-53 36.017143400907216 39.0 36.0 41.0 29.0 41.0 54-55 35.83085458306595 39.0 35.0 40.0 28.5 41.0 56-57 35.6070813420924 39.0 35.0 40.0 28.0 41.0 58-59 35.400416116355736 39.0 35.0 40.0 28.0 41.0 60-61 35.116366255077125 38.0 34.5 40.0 27.0 41.0 62-63 34.807582766604725 38.0 34.0 40.0 26.0 41.0 64-65 34.459070255857526 38.0 34.0 40.0 26.0 41.0 66-67 34.061367837868595 37.0 34.0 40.0 25.5 41.0 68-69 33.63579011898192 37.0 33.0 39.0 24.5 40.5 70-71 33.26745350370737 36.0 32.5 39.0 23.5 40.0 72-73 32.77943585677794 36.0 32.0 38.5 22.0 40.0 74-75 32.25729877269106 35.0 31.5 38.0 21.5 39.5 76-77 30.91551240841567 34.0 30.0 36.0 20.5 39.0 78-79 31.532011598848914 35.0 31.0 37.0 20.5 39.0 80-81 31.307324546848236 35.0 31.0 36.0 20.0 38.5 82-83 30.910220816146577 34.0 31.0 36.0 18.0 37.5 84-85 30.474895779637173 34.0 31.0 35.5 16.5 37.0 86-87 30.03663564522901 34.0 30.0 35.0 10.0 36.5 88-89 29.627248783258942 34.0 30.0 35.0 6.5 36.0 90-91 29.093200117442173 34.0 29.0 35.0 2.0 36.0 92-93 28.632186040639965 34.0 29.0 35.0 2.0 35.0 94-95 28.150520121535433 33.5 29.0 35.0 2.0 35.0 96-97 27.58692729009842 33.0 27.5 35.0 2.0 35.0 98-99 26.720227501169163 33.0 25.5 34.5 2.0 35.0 100-101 24.035676406652122 30.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34853560606794787 1101 2 0.3816156475733905 1101 3 0.3255317188015425 1101 4 0.18454417816660396 1101 5 0.09827090656047233 1101 6 0.09751834547968485 1101 7 0.23787122148042528 1101 8 0.12722681365418254 1101 9 0.34354611744259245 1101 10-11 0.2006320469891847 1101 12-13 0.38159755090189407 1101 14-15 0.3163236035310959 1101 16-17 0.3795535200773301 1101 18-19 0.34524865474047317 1101 20-21 0.4063930670845366 1101 22-23 0.4329862981297623 1101 24-25 0.29243424812369057 1101 26-27 0.18931756290881907 1101 28-29 0.397068805523233 1101 30-31 0.3711996839065108 1101 32-33 0.2560661831383655 1101 34-35 0.4279589494300069 1101 36-37 0.29280796038047185 1101 38-39 0.45788857366449776 1101 40-41 0.33476370192279603 1101 42-43 0.6918118725862925 1101 44-45 0.4588727845273226 1101 46-47 0.49494938290591506 1101 48-49 0.476992727129371 1101 50-51 0.46412721190913686 1101 52-53 0.4731568289791994 1101 54-55 0.5200400365435911 1101 56-57 0.7898653574767991 1101 58-59 0.7568574600175637 1101 60-61 0.5300761540102883 1101 62-63 0.4871260037608991 1101 64-65 0.3879094299493815 1101 66-67 0.37126191192943736 1101 68-69 0.47575788473827885 1101 70-71 0.4726175457007784 1101 72-73 0.3728458068832552 1101 74-75 0.34095769920519814 1101 76-77 0.3672357315338637 1101 78-79 0.36577221354908573 1101 80-81 0.321271049548308 1101 82-83 0.26123234521503846 1101 84-85 0.1003709481985453 1101 86-87 0.2367582950760294 1101 88-89 0.3850140122145227 1101 90-91 0.5579976911698594 1101 92-93 0.5498268751341762 1101 94-95 0.5534442636032502 1101 96-97 0.6525101280317038 1101 98-99 0.7777144382415706 1101 100-101 1.004968397408117 1102 1 0.370909567602034 1102 2 0.36437426826304176 1102 3 0.31951904856899915 1102 4 0.15448462048669853 1102 5 0.11501533962447041 1102 6 0.26394222924737676 1102 7 0.1618232718578767 1102 8 0.15380774760964755 1102 9 0.27534309024331804 1102 10-11 0.27957442398550114 1102 12-13 0.3282668730065197 1102 14-15 0.44364236989049033 1102 16-17 0.3893482926580347 1102 18-19 0.3970695264828237 1102 20-21 0.35224299690510463 1102 22-23 0.23426090834940538 1102 24-25 0.23818744413294723 1102 26-27 0.2520371107256736 1102 28-29 0.2416099837789858 1102 30-31 0.35910795749249047 1102 32-33 0.5214506526197908 1102 34-35 0.421523305865648 1102 36-37 0.4586362673491067 1102 38-39 0.4322711843393563 1102 40-41 0.3267537629978676 1102 42-43 0.5355677999031556 1102 44-45 0.521249022151089 1102 46-47 0.62222262747175 1102 48-49 0.6178030150547116 1102 50-51 0.596673397562185 1102 52-53 0.5808120772626495 1102 54-55 0.4442784948721794 1102 56-57 0.4515113496622263 1102 58-59 0.41639806925090284 1102 60-61 0.39127451522572443 1102 62-63 0.531492681808011 1102 64-65 0.565099217893696 1102 66-67 0.34401522174317734 1102 68-69 0.48409216644231634 1102 70-71 0.4081416153491233 1102 72-73 0.4785625854576665 1102 74-75 0.4696079780261826 1102 76-77 0.34833849698282293 1102 78-79 0.6554254892214786 1102 80-81 0.4251347117765967 1102 82-83 0.415501910102563 1102 84-85 0.5005682093039674 1102 86-87 0.5999368543112631 1102 88-89 0.6251490602020802 1102 90-91 0.5625536640085222 1102 92-93 0.9137489569975337 1102 94-95 1.1240637393439243 1102 96-97 1.244898504800414 1102 98-99 0.8717890181133505 1102 100-101 1.4956307395043957 1103 1 0.26758058518610994 1103 2 0.26683943439380187 1103 3 0.18407109862931037 1103 4 0.13088708388416137 1103 5 0.04094008643998848 1103 6 0.1167998622775599 1103 7 0.27912117528165936 1103 8 0.13261913357139576 1103 9 0.20405171673297673 1103 10-11 0.13244266900935742 1103 12-13 0.16620227426368217 1103 14-15 0.26808285252396047 1103 16-17 0.3239819489497151 1103 18-19 0.17266300222911468 1103 20-21 0.25640785068952 1103 22-23 0.2749791280813412 1103 24-25 0.2933397439288399 1103 26-27 0.12016540265454267 1103 28-29 0.3626839882855464 1103 30-31 0.34829433593741044 1103 32-33 0.24043621677106586 1103 34-35 0.24151736314919248 1103 36-37 0.2571905600774045 1103 38-39 0.2342089343844549 1103 40-41 0.21802681313246808 1103 42-43 0.2487379915203931 1103 44-45 0.34461769342335913 1103 46-47 0.3671580534902361 1103 48-49 0.39462217607049155 1103 50-51 0.48735040738535673 1103 52-53 0.4223307950508257 1103 54-55 0.590020078676865 1103 56-57 0.7263531425231804 1103 58-59 0.6845490000996151 1103 60-61 0.6719192999614307 1103 62-63 0.5255393533562014 1103 64-65 0.3795308218257958 1103 66-67 0.6515313431302303 1103 68-69 0.7595070069617336 1103 70-71 0.7632613716977517 1103 72-73 0.6374205934449577 1103 74-75 0.616355932564403 1103 76-77 0.6551630701153748 1103 78-79 0.7005229250100768 1103 80-81 0.3505111722522294 1103 82-83 0.6198234676602503 1103 84-85 0.5419461413093885 1103 86-87 0.8428029415630718 1103 88-89 0.6672662944335421 1103 90-91 0.5086093628259896 1103 92-93 0.3760622301254912 1103 94-95 0.41378354733758727 1103 96-97 0.6533842086762682 1103 98-99 0.7642170068929381 1103 100-101 0.8543585737026085 1104 1 0.24792282097784124 1104 2 0.2581702724691439 1104 3 0.12140901674563054 1104 4 -0.005114620194468955 1104 5 0.11848918858665058 1104 6 0.046280084484003225 1104 7 0.1402861023680586 1104 8 0.1589647301848771 1104 9 0.02939041926644137 1104 10-11 0.15157298030801059 1104 12-13 0.01308709004398878 1104 14-15 0.15182344036346507 1104 16-17 0.26792148120717485 1104 18-19 0.20251142713976833 1104 20-21 0.2945670400349698 1104 22-23 0.2739377466956938 1104 24-25 0.10062163707905825 1104 26-27 0.022475037799132735 1104 28-29 0.05160849151619118 1104 30-31 0.29611538063228693 1104 32-33 0.2693987912822422 1104 34-35 0.20224917025019806 1104 36-37 0.289040601157879 1104 38-39 0.37403129094938237 1104 40-41 0.1655824408183264 1104 42-43 0.20833712730908616 1104 44-45 0.19683282658831303 1104 46-47 0.22282022666384904 1104 48-49 -0.027989324431594298 1104 50-51 -0.11638876038863089 1104 52-53 -0.2610757392792564 1104 54-55 -0.21322984271236578 1104 56-57 0.06073631001314794 1104 58-59 0.1943406900113942 1104 60-61 0.14913231993364207 1104 62-63 0.3727436838798255 1104 64-65 0.2953970110799631 1104 66-67 0.31243998220028857 1104 68-69 0.3109150695528129 1104 70-71 0.16662929376995805 1104 72-73 0.2068753220941062 1104 74-75 0.12494732899597949 1104 76-77 -0.011579671490174803 1104 78-79 0.11534435177019375 1104 80-81 0.20710991245124077 1104 82-83 0.3090150784540988 1104 84-85 0.22316002684534197 1104 86-87 0.2571814431234536 1104 88-89 0.48597126229610765 1104 90-91 0.37530071158394307 1104 92-93 0.22181099348509647 1104 94-95 0.2551520847715274 1104 96-97 0.5748985395042006 1104 98-99 0.4995353889649472 1104 100-101 0.7555811824982221 1105 1 0.298954223919516 1105 2 0.25943599665810524 1105 3 0.2705437806718436 1105 4 0.12447606407550182 1105 5 -0.011059304877015563 1105 6 0.23977036761676374 1105 7 0.25675869132575 1105 8 0.2467670435392364 1105 9 0.297100821572009 1105 10-11 0.25015666739502507 1105 12-13 0.24842740757848958 1105 14-15 0.2167599594272147 1105 16-17 0.2576967015730034 1105 18-19 0.3016095175189619 1105 20-21 0.27865786061369846 1105 22-23 0.39434219425577055 1105 24-25 0.29915696701115735 1105 26-27 0.18879606439881513 1105 28-29 0.1857590283130719 1105 30-31 0.22988068291318342 1105 32-33 0.270625570111541 1105 34-35 0.19616882597321705 1105 36-37 0.08499612555714009 1105 38-39 0.22025553833030642 1105 40-41 0.279005932659274 1105 42-43 0.09056311424940588 1105 44-45 0.1518768997536668 1105 46-47 0.17958114110088985 1105 48-49 0.18033102711518012 1105 50-51 0.06099112563128273 1105 52-53 0.2189777456813431 1105 54-55 0.07140231836564226 1105 56-57 0.1511609442513162 1105 58-59 0.039839580068651514 1105 60-61 0.23498249713502872 1105 62-63 0.35664140038357317 1105 64-65 0.335181318696371 1105 66-67 0.34988756823707945 1105 68-69 0.17103956488586647 1105 70-71 0.16815465368999583 1105 72-73 0.2184680574863478 1105 74-75 0.10473306460891507 1105 76-77 0.042604682860670096 1105 78-79 0.22803274994755185 1105 80-81 0.16663432470085482 1105 82-83 0.40557036139213665 1105 84-85 0.2899480732162871 1105 86-87 0.41121196330364285 1105 88-89 0.43503884547690674 1105 90-91 0.21701216541421786 1105 92-93 0.537669219394143 1105 94-95 0.6356352668670482 1105 96-97 0.7824448520277265 1105 98-99 0.9936822259526501 1105 100-101 1.4704962816141638 1106 1 0.11753375683483824 1106 2 0.18982116626514767 1106 3 0.15190323335971812 1106 4 0.07269820840700447 1106 5 0.025955014033549162 1106 6 0.20611184053026932 1106 7 0.1118949351756271 1106 8 0.09902782166752644 1106 9 0.09668800075960604 1106 10-11 0.07954353761611088 1106 12-13 -0.1183534988098458 1106 14-15 0.1928329040858614 1106 16-17 0.2505713234784679 1106 18-19 0.009556642702335694 1106 20-21 0.09530310733254765 1106 22-23 0.24975267834659576 1106 24-25 0.32407063813057846 1106 26-27 0.2490786129831477 1106 28-29 0.3029228974047342 1106 30-31 0.18044766244089772 1106 32-33 0.15583412251252327 1106 34-35 0.11865295573915091 1106 36-37 0.08399328254589022 1106 38-39 0.1837053182890216 1106 40-41 0.19081903387827026 1106 42-43 0.13788497168342673 1106 44-45 0.2551924480394163 1106 46-47 0.34637438018645383 1106 48-49 0.4073419058523129 1106 50-51 0.3851885266776165 1106 52-53 0.15585380856511932 1106 54-55 0.37825280712990406 1106 56-57 0.27156701453470333 1106 58-59 0.35890430725640243 1106 60-61 0.19434788249935764 1106 62-63 0.33120845780201336 1106 64-65 0.3432413854227505 1106 66-67 0.27037899376706775 1106 68-69 0.1947215890060221 1106 70-71 0.2844000613829962 1106 72-73 0.25366817172607625 1106 74-75 0.06290737105697275 1106 76-77 0.1393141245140299 1106 78-79 0.33406149162297893 1106 80-81 0.31331390824514216 1106 82-83 0.17704872724484488 1106 84-85 0.46290766091474467 1106 86-87 0.46170299938002657 1106 88-89 -0.2974146472727668 1106 90-91 -0.10682336833193418 1106 92-93 0.13716325426322484 1106 94-95 0.26724461894608 1106 96-97 0.40244848692796253 1106 98-99 0.7897562091542234 1106 100-101 0.555265725084972 1107 1 0.3833097335047668 1107 2 0.42273445444278224 1107 3 0.32496996960095714 1107 4 0.16028648961023606 1107 5 0.2719044979280554 1107 6 0.3082384956087054 1107 7 0.26241657501203974 1107 8 0.22403163358578126 1107 9 0.42807640868794294 1107 10-11 0.35189690865072265 1107 12-13 0.3898700795140613 1107 14-15 0.41291668722555386 1107 16-17 0.49140210706203646 1107 18-19 0.5020118681928238 1107 20-21 0.5449519025969636 1107 22-23 0.5766249514132156 1107 24-25 0.45502668981906425 1107 26-27 0.43701747772048094 1107 28-29 0.5943742612808478 1107 30-31 0.557462755378296 1107 32-33 0.5995431020365984 1107 34-35 0.6578372165263175 1107 36-37 0.6323797963743942 1107 38-39 0.7095077694371739 1107 40-41 0.6988568164825963 1107 42-43 0.8252890384072415 1107 44-45 0.8895566370156871 1107 46-47 0.6944364009436228 1107 48-49 0.7648219059878514 1107 50-51 0.737518201698883 1107 52-53 0.5011492237592492 1107 54-55 0.7037437539652203 1107 56-57 0.6293847223915492 1107 58-59 0.5483380086798562 1107 60-61 0.6768328240104182 1107 62-63 0.8044877578999419 1107 64-65 0.730469030922265 1107 66-67 0.7063082716711477 1107 68-69 0.86191910058578 1107 70-71 0.7392008759475743 1107 72-73 0.7613552578505747 1107 74-75 0.769695183309544 1107 76-77 0.5162529790862429 1107 78-79 0.6285578722771845 1107 80-81 0.6456984974784774 1107 82-83 0.6343930795632247 1107 84-85 0.6875680048796582 1107 86-87 0.6986241666515198 1107 88-89 0.7624341635781526 1107 90-91 0.8069476522795291 1107 92-93 0.8921969629152038 1107 94-95 1.1292458365010098 1107 96-97 1.1834760897339898 1107 98-99 1.3786354600078106 1107 100-101 1.3316156806408266 1108 1 0.16381305930647727 1108 2 0.17689443605910782 1108 3 -0.019067762677465794 1108 4 0.07131076583114293 1108 5 -0.03688503918398567 1108 6 0.008892426106363871 1108 7 0.05027232973559137 1108 8 0.04718653369735648 1108 9 0.23856342002073205 1108 10-11 0.1178300307659299 1108 12-13 0.14419226999925883 1108 14-15 0.20369272148905537 1108 16-17 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Count 2 1.0 3 7.0 4 10.0 5 15.0 6 37.0 7 83.0 8 208.0 9 419.0 10 832.0 11 1374.0 12 2254.0 13 3276.0 14 4199.0 15 6070.0 16 8222.0 17 11462.0 18 9765.0 19 5510.0 20 4431.0 21 4296.0 22 4805.0 23 5321.0 24 6536.0 25 7672.0 26 9435.0 27 11675.0 28 14687.0 29 18644.0 30 23812.0 31 29730.0 32 37806.0 33 48963.0 34 66092.0 35 93537.0 36 140755.0 37 231095.0 38 213022.0 39 19561.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.4891882972465 22.60800941271003 15.968318195514614 21.934484094528862 2 14.321919701306687 22.520014422051585 41.45479098067082 21.70327489597091 3 17.445613659251297 26.331720575619656 32.59890533950638 23.623760425622667 4 11.475425514313558 19.461045487975042 40.607794249710935 28.45573474800046 5 11.463944508378558 39.41992467572968 36.75774647078913 12.358384345102632 6 27.938803830450997 40.584781675195885 18.7580394808444 12.718375013508718 7 25.568442102826932 35.04577662460873 22.754038030988283 16.631743241576057 8 23.850037441864693 37.27803860098449 21.334211918085042 17.53771203906578 9 25.01173943522557 17.322720182551805 23.161738335400685 34.50380204682194 10-11 22.563970388874356 28.53229254782208 29.928688276482717 18.97504878682084 12-13 23.35192266125469 26.798998069974427 29.847778557779304 20.001300710991583 14-15 20.830970303771636 28.35233798862112 28.464950493534463 22.351741214072785 16-17 18.960215986480762 31.567508686225697 29.616275878162355 19.855999449131186 18-19 18.67053167447861 30.017616325609374 32.680722747534716 18.631129252377296 20-21 19.693129728649293 28.952364193144554 32.77196039482757 18.582545683378584 22-23 19.407313044717394 29.17150833340506 32.130735043912125 19.29044357796542 24-25 19.182093703167784 29.226029316549685 32.734614167083485 18.857262813199046 26-27 19.221692868937005 29.37174324596374 32.74640630327305 18.660157581826205 28-29 18.741924593515957 29.33738390781293 32.986056982841646 18.934634515829472 30-31 19.328609505738694 29.054552270851485 32.800651478881925 18.816186744527894 32-33 19.085548205324873 29.288097695053946 32.579825768610235 19.04652833101095 34-35 19.473260387846075 29.295174829120686 32.50016019188597 18.73140459114727 36-37 19.54618355981756 28.986267490802113 32.158305925378315 19.309243024002004 38-39 18.94754409102342 29.687286311196885 32.22496487733127 19.140204720448423 40-41 19.373409422815943 29.340835167189326 32.459251098161744 18.826504311832988 42-43 19.266132268171056 29.309562308788976 32.16568170720567 19.258623715834293 44-45 19.593533217835727 29.394029669464206 31.863520273368778 19.148916839331285 46-47 19.65673987037368 29.60962911035643 31.249611474903432 19.484019544366458 48-49 19.561437652839793 29.55444659202522 31.369492387777214 19.51462336735777 50-51 19.514862459724892 29.7412733676925 30.935109375194266 19.808754797388346 52-53 19.709445252842688 30.178205190123954 30.729504633851384 19.382844923181977 54-55 20.03005869239428 29.70947408286559 30.612095587215638 19.648371637524495 56-57 19.95139730361192 30.11435309734598 30.292237818482988 19.642011780559113 58-59 19.886172905861685 29.929152149775113 30.49121048639995 19.69346445796326 60-61 20.048755715503034 30.013599573841766 30.4341630476052 19.503481663049996 62-63 19.990417177925845 29.667154733885415 30.786107012005303 19.556321076183437 64-65 19.929066076522947 29.657591039200625 30.53209528117741 19.88124760309902 66-67 19.814923380460034 29.891088644929663 30.273588613847657 20.020399360762646 68-69 19.848597077046595 29.527730185091055 30.523024366871155 20.100648370991195 70-71 19.86604132855021 29.67528387436748 30.808964242301943 19.649710554780363 72-73 20.184225450713022 29.269878856679426 30.71026691315496 19.835628779452595 74-75 19.848444130330208 29.802194102834584 30.288507977555923 20.06085378927929 76-77 19.638951398259984 29.64209785381128 30.68669240575696 20.03225834217178 78-79 19.503338207629724 29.605612358588818 30.509811872561855 20.3812375612196 80-81 19.53376943822804 29.653889558348155 30.364329297450315 20.44801170597349 82-83 19.55711299031938 29.939775859463612 30.38258635761108 20.120524792605927 84-85 19.567795443068967 29.618904071858122 30.251928050068095 20.56137243500482 86-87 19.472112744483898 29.62340083070252 30.31380394999718 20.5906824748164 88-89 19.861355118154666 29.175533008614018 30.42971592957678 20.533395943654536 90-91 19.496182512487835 29.111086136769593 30.736883887644158 20.655847463098418 92-93 19.625341433369805 29.203169113765213 30.80293269267554 20.368556760189442 94-95 19.561437652839793 28.959919511945532 30.74794787021301 20.730694965001657 96-97 19.864893685188036 28.855005781253436 30.32078544711707 20.959315086441453 98-99 19.4811814960982 28.785398713569588 30.301026246489478 21.432393543842736 100-101 19.3766646691036 28.71287270772464 30.835901230151308 21.074561393020456 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 757.0 1 485.5 2 279.0 3 359.5 4 291.0 5 259.5 6 473.0 7 912.0 8 1376.0 9 1554.5 10 1310.5 11 1015.0 12 942.0 13 973.0 14 1049.0 15 1104.0 16 1218.5 17 1389.0 18 1564.5 19 1760.0 20 2097.5 21 2586.0 22 3161.0 23 3822.5 24 4755.5 25 6411.5 26 9788.5 27 13569.0 28 15071.0 29 17266.0 30 21490.5 31 25434.0 32 30313.0 33 36037.0 34 42145.0 35 46788.5 36 49595.5 37 51944.5 38 49984.0 39 46610.5 40 45669.0 41 44475.0 42 42300.5 43 41421.5 44 40776.5 45 38726.0 46 36610.0 47 33922.5 48 33064.0 49 30958.0 50 26493.0 51 23092.5 52 19991.0 53 17432.0 54 15126.5 55 12794.0 56 10674.5 57 8741.5 58 6798.5 59 5253.0 60 4063.0 61 3031.5 62 2190.0 63 1474.0 64 919.0 65 597.5 66 378.0 67 255.0 68 201.5 69 151.5 70 114.5 71 74.5 72 62.5 73 67.0 74 52.5 75 35.0 76 24.5 77 13.0 78 8.5 79 8.0 80 6.5 81 5.5 82 2.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021613949987615014 2 0.0 3 0.0 4 3.825477873914162E-4 5 0.026204523436312008 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.781847342392702E-5 12-13 0.003729840927066308 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.563694684785406E-4 24-25 0.0 26-27 5.260032076631972E-4 28-29 7.650955747828324E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.563694684785406E-4 42-43 0.014010812713210617 44-45 4.781847342392702E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.781847342392702E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.781847342392702E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.563694684785405E-5 82-83 0.0025821975648920594 84-85 4.781847342392703E-4 86-87 0.0 88-89 0.0 90-91 0.009898423998752895 92-93 0.0027734714585877676 94-95 0.0 96-97 0.0 98-99 0.0013867357292938838 100-101 0.017262468906037658 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1045621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.268841921272674 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36688227442103 43.312468282879614 2 13.133677334427066 14.517662728829025 3 4.075326478506659 6.75715724754484 4 1.631054589565574 3.6058599310266395 5 0.7848039983040169 2.168760406072373 6 0.4126429115074438 1.368377750762317 7 0.2774816611030403 1.073526304448926 8 0.18417337743153903 0.8143239426696494 9 0.13152330502680254 0.654222667904071 >10 0.790489347320013 8.920118344827705 >50 0.12164399314189261 4.6800305258188475 >100 0.08508103427016134 8.792203319677734 >500 0.004001766147389749 1.506668808754158 >1k 0.001043938994971239 1.2560702055110295 >5k 1.7398983249520647E-4 0.5725495332730569 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5954 0.569422381532123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4996 0.47780218645187883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3524 0.3370246006918377 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1177 0.11256468643992422 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1160 0.11093885834351071 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1152 0.11017376276872787 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.10070570503079032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.563694684785405E-5 0.0 10-11 0.0 0.0 0.0 9.563694684785405E-5 0.0 12-13 0.0 0.0 0.0 9.563694684785405E-5 0.0 14-15 0.0 0.0 0.0 9.563694684785405E-5 0.0 16-17 0.0 0.0 0.0 9.563694684785405E-5 0.0 18-19 0.0 0.0 0.0 9.563694684785405E-5 0.0 20-21 0.0 0.0 0.0 1.912738936957081E-4 0.0 22-23 0.0 0.0 0.0 3.825477873914162E-4 0.0 24-25 0.0 0.0 0.0 6.694586279349783E-4 0.0 26-27 0.0 0.0 0.0 0.0010520064153263944 0.0 28-29 0.0 0.0 0.0 0.002390923671196351 0.0 30-31 0.0 0.0 0.0 0.006168583071686586 0.0 32-33 0.0 0.0 0.0 0.012289347669949244 0.0 34-35 0.0 0.0 0.0 0.019079570896146883 0.0 36-37 0.0 0.0 0.0 0.02682616359082306 0.0 38-39 0.0 0.0 0.0 0.03428584544495568 0.0 40-41 0.0 0.0 0.0 0.038493871106261256 0.0 42-43 0.0 0.0 0.0 0.045666642119850304 0.0 44-45 0.0 0.0 0.0 0.052552502292895806 0.0 46-47 0.0 0.0 0.0 0.06230747087137692 0.0 48-49 0.0 0.0 0.0 0.07560100648322862 0.0 50-51 0.0 0.0 0.0 0.08784253567975395 0.0 52-53 0.0 0.0 0.0 0.10897830093312968 0.0 54-55 0.0 0.0 0.0 0.15564913099488248 0.0 56-57 0.0 0.0 0.0 0.23598416634707986 0.0 58-59 0.0 0.0 0.0 0.3174668450614515 0.0 60-61 0.0 0.0 0.0 0.3945024057473979 0.0 62-63 0.0 0.0 0.0 0.4846880466249243 0.0 64-65 0.0 0.0 0.0 0.6092551698942543 0.0 66-67 0.0 0.0 0.0 0.7886700821808283 0.0 68-69 0.0 0.0 0.0 0.987547113150941 0.0 70-71 0.0 0.0 0.0 1.1779602743250184 0.0 72-73 0.0 0.0 0.0 1.3619179415868656 0.0 74-75 0.0 0.0 0.0 1.5818351008635059 0.0 76-77 0.0 0.0 0.0 1.8396244910918966 0.0 78-79 0.0 0.0 0.0 2.0689618896330506 0.0 80-81 0.0 0.0 0.0 2.300642393371977 0.0 82-83 0.0 0.0 0.0 2.5502548246448757 0.0 84-85 0.0 0.0 0.0 2.8758986286618193 0.0 86-87 0.0 0.0 0.0 3.271357403877696 0.0 88-89 0.0 0.0 0.0 3.7340967712010373 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3170 0.0 39.133987 1 TAGCACG 65 1.3260706E-4 36.547974 4 GTATCAA 5040 0.0 33.57317 1 GCGAGCT 75 3.077878E-4 31.667337 9 CCGCGAT 60 0.0039503807 31.667336 9 GCCGCGA 65 0.005843231 29.231388 8 ATGGGCG 115 3.8563066E-6 28.92057 5 TCAACGC 5940 0.0 28.155476 4 GGCGAGC 85 6.4059976E-4 27.941767 8 CATTCCG 290 0.0 27.845415 9 CAACGCA 6025 0.0 27.83712 5 ATCAACG 6070 0.0 27.552475 3 AACGCAG 6110 0.0 27.441984 6 TATCAAC 6265 0.0 26.846571 2 ACGCAGA 6890 0.0 24.267567 7 GTACATG 3645 0.0 23.993475 1 CGCAGAG 6970 0.0 23.92088 8 TACATGG 3690 0.0 22.790482 2 GAGTACT 4745 0.0 22.424078 12-13 GCAGAGT 7510 0.0 22.137619 9 >>END_MODULE