FastQCFastQC Report
Thu 26 May 2016
SRR937649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026302
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.5102786509234124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.39695917965667027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.27487035979662905No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14650.14274550765759006No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12660.12335550354573994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47650.036.1157341
GGTATCA29000.034.424851
TCAACGC57400.029.3884264
CTCGGTA650.005843999329.2305439
CAACGCA57900.029.0511535
ATCAACG58950.028.8561173
TATCAAC59100.028.6304682
AACGCAG59300.028.27556
CATTCCG3550.026.7603559
GTACATG40700.026.5144271
ACGCAGA65600.025.4876087
CGCAGAG65550.025.4345868
ACATGGG40600.025.3964143
TACATGG41900.025.2962882
AGTACTT39100.023.68907712-13
GCAGAGT68700.023.3695439
CATGGGG14850.023.0391244
GAGTACT38850.022.86340112-13
CGCGGCA550.00287825621.59074236-37
AGAGTAC63050.020.8300210-11
Produced by FastQC (version 0.11.5)