##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937649_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026302 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765299102993076 33.0 31.0 34.0 30.0 34.0 2 31.906278074095148 33.0 31.0 34.0 30.0 34.0 3 31.931549387996906 33.0 31.0 34.0 30.0 34.0 4 35.538927138405654 37.0 35.0 37.0 33.0 37.0 5 35.548603627392325 37.0 35.0 37.0 33.0 37.0 6 35.60735436547917 37.0 35.0 37.0 33.0 37.0 7 35.5638583964564 37.0 35.0 37.0 33.0 37.0 8 35.55254691114311 37.0 35.0 37.0 33.0 37.0 9 37.18514433373412 39.0 37.0 39.0 34.0 39.0 10-11 37.08025610395381 39.0 37.0 39.0 33.0 39.0 12-13 36.987108083195785 39.0 37.0 39.0 33.0 39.0 14-15 38.16410764083086 40.0 38.0 41.0 33.0 41.0 16-17 38.10010211419251 40.0 38.0 41.0 33.0 41.0 18-19 38.07911267833445 40.0 38.0 41.0 33.0 41.0 20-21 38.021755779487904 40.0 38.0 41.0 33.0 41.0 22-23 37.835073886633765 40.0 37.5 41.0 33.0 41.0 24-25 37.696735951016365 40.0 37.0 41.0 32.0 41.0 26-27 37.659182190037626 40.0 37.0 41.0 32.5 41.0 28-29 37.72147671932823 40.0 37.0 41.0 33.0 41.0 30-31 37.56564149733704 40.0 37.0 41.0 32.5 41.0 32-33 37.35623042730113 40.0 36.5 41.0 32.0 41.0 34-35 37.34966413394888 40.0 37.0 41.0 32.0 41.0 36-37 37.273963706589285 40.0 37.0 41.0 32.0 41.0 38-39 37.16257300482704 40.0 36.0 41.0 31.0 41.0 40-41 36.975318181198126 39.5 36.0 41.0 31.0 41.0 42-43 36.755802385652565 39.0 36.0 41.0 30.5 41.0 44-45 36.51983042028564 39.0 36.0 41.0 30.0 41.0 46-47 36.20264259447998 39.0 36.0 41.0 30.0 41.0 48-49 35.89221593643976 39.0 35.5 41.0 28.5 41.0 50-51 34.99680552118187 38.0 34.0 40.0 27.0 40.5 52-53 35.12205033216344 38.5 35.0 40.0 27.5 40.5 54-55 35.521297337430894 39.0 35.0 40.0 27.5 41.0 56-57 35.38953397732831 39.0 35.0 40.0 27.0 41.0 58-59 35.21150938027988 39.0 35.0 40.0 26.0 41.0 60-61 34.95098177729362 38.5 34.5 40.0 26.0 41.0 62-63 34.638455347451334 38.0 34.0 40.0 25.5 41.0 64-65 34.333121732199686 38.0 34.0 40.0 24.5 41.0 66-67 33.961154708847886 37.5 34.0 40.0 23.0 41.0 68-69 33.50224787635608 37.0 33.0 39.0 22.0 41.0 70-71 33.10440055656132 36.5 32.5 39.0 21.5 40.5 72-73 32.6546041028859 36.0 32.0 39.0 20.0 40.0 74-75 32.19055697056032 35.5 32.0 38.0 18.5 40.0 76-77 31.687523263133073 35.0 31.0 37.5 17.0 39.0 78-79 31.150566792230748 35.0 31.0 37.0 10.5 39.0 80-81 30.60814458122463 34.5 30.5 36.0 7.5 39.0 82-83 30.06408493796173 34.0 30.0 36.0 4.5 37.5 84-85 29.5761691003233 34.0 29.0 35.0 2.0 37.0 86-87 29.106639663568814 34.0 29.0 35.0 2.0 36.5 88-89 28.602204809110766 34.0 29.0 35.0 2.0 36.0 90-91 28.117994508439036 34.0 27.5 35.0 2.0 36.0 92-93 27.5875931256102 33.5 26.5 35.0 2.0 35.0 94-95 26.959878281441526 33.0 25.5 35.0 2.0 35.0 96-97 26.26232288351772 32.5 25.0 35.0 2.0 35.0 98-99 25.36196996595544 32.0 21.5 34.0 2.0 35.0 100-101 22.926973736775334 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46588417622467304 1101 2 0.4740704068814736 1101 3 0.35374326034675363 1101 4 0.36929181100288133 1101 5 0.43092384521745686 1101 6 0.4731675320304447 1101 7 0.6059776487283557 1101 8 0.49338637251267414 1101 9 0.5917063866032422 1101 10-11 0.5096398700230651 1101 12-13 0.6386418513206991 1101 14-15 0.7240364892482987 1101 16-17 0.9165659295760946 1101 18-19 0.8394326723278951 1101 20-21 1.0497264234476091 1101 22-23 0.9943187892941268 1101 24-25 0.996016782938483 1101 26-27 1.116357215510007 1101 28-29 0.8121711985033926 1101 30-31 0.7953490875944738 1101 32-33 1.000754802527922 1101 34-35 1.1089417408098043 1101 36-37 1.0087987162278438 1101 38-39 1.1093954728832855 1101 40-41 1.2287238011639872 1101 42-43 1.0196459399073987 1101 44-45 1.1202763051626121 1101 46-47 1.1095416713526234 1101 48-49 1.0070542033444525 1101 50-51 1.141980994795631 1101 52-53 1.0750501196881928 1101 54-55 1.359764497631609 1101 56-57 1.2734393213137736 1101 58-59 1.4084707280235946 1101 60-61 1.476842576274521 1101 62-63 1.5035107151696039 1101 64-65 1.6440625834740459 1101 66-67 1.6170741818323648 1101 68-69 1.5694818651640219 1101 70-71 1.6620556702319078 1101 72-73 1.5712725272378947 1101 74-75 1.6810218492646527 1101 76-77 1.7010803837231698 1101 78-79 1.69017237454662 1101 80-81 1.6889059973431237 1101 82-83 1.8308980619687674 1101 84-85 1.9832792610701482 1101 86-87 2.0656164830565302 1101 88-89 2.085036714757422 1101 90-91 2.0189188701056615 1101 92-93 2.021822365457062 1101 94-95 2.205050106798719 1101 96-97 2.605240326668955 1101 98-99 3.1681736992981406 1101 100-101 3.3719410203501106 1102 1 0.2885033085570292 1102 2 0.25363467548366714 1102 3 0.35795706997222254 1102 4 0.3895769221298053 1102 5 0.19597045448238504 1102 6 0.21533527794471752 1102 7 0.22182717077583902 1102 8 0.19549268433221556 1102 9 0.3602563996490886 1102 10-11 0.39782922460927495 1102 12-13 0.40602841172298554 1102 14-15 0.4249467337986843 1102 16-17 0.4194152618657583 1102 18-19 0.5155617313662972 1102 20-21 0.6029878860060123 1102 22-23 0.5682466576021952 1102 24-25 0.6181366150028111 1102 26-27 0.47748591875249957 1102 28-29 0.284847258182225 1102 30-31 0.6108831372635848 1102 32-33 0.42728413197121995 1102 34-35 0.6868563023506411 1102 36-37 0.5874770530005406 1102 38-39 0.7092994079861015 1102 40-41 0.6924533725485063 1102 42-43 0.5763918304645799 1102 44-45 0.8673884283154436 1102 46-47 0.7772431111401019 1102 48-49 0.929357863298435 1102 50-51 0.7356614663438208 1102 52-53 0.7374353755054628 1102 54-55 0.814828066501839 1102 56-57 0.8047327580651213 1102 58-59 0.46951463323956233 1102 60-61 0.7228967995720339 1102 62-63 0.8031952427912259 1102 64-65 0.619233958506662 1102 66-67 0.7062437541656763 1102 68-69 0.8404933929516787 1102 70-71 0.7292904243769343 1102 72-73 0.5424708478811766 1102 74-75 0.5535947281279974 1102 76-77 0.6052076163425433 1102 78-79 0.7016550947249911 1102 80-81 0.6604073375077419 1102 82-83 0.6480936785630895 1102 84-85 0.9112892945149618 1102 86-87 0.7126088215844852 1102 88-89 0.7732598699555986 1102 90-91 0.9513816894324947 1102 92-93 0.980968099464036 1102 94-95 1.203871236332155 1102 96-97 1.4045038291239464 1102 98-99 1.5753370570627894 1102 100-101 2.2761079835133735 1103 1 0.380357798279352 1103 2 0.3641016787292557 1103 3 0.4710611089192085 1103 4 0.39826787056110646 1103 5 0.328336123432976 1103 6 0.28273520582066425 1103 7 0.33706337705063305 1103 8 0.32463530964776055 1103 9 0.39914478767649797 1103 10-11 0.354417306109454 1103 12-13 0.5049735974424223 1103 14-15 0.5065235459155275 1103 16-17 0.47377876709475686 1103 18-19 0.3688321064113751 1103 20-21 0.44713456829956044 1103 22-23 0.5219137149408226 1103 24-25 0.7702642745772792 1103 26-27 0.5869589623875484 1103 28-29 0.5950798582543513 1103 30-31 0.5289614820165625 1103 32-33 0.517594716176049 1103 34-35 0.5403633344458427 1103 36-37 0.6435422351137703 1103 38-39 0.5703546730420044 1103 40-41 0.7016266505867392 1103 42-43 0.7491131611533604 1103 44-45 0.5314143028195915 1103 46-47 0.5324833743928892 1103 48-49 0.43888499997351005 1103 50-51 0.3747436877646564 1103 52-53 0.4094061652638459 1103 54-55 0.46417444226815263 1103 56-57 0.3248914309825395 1103 58-59 0.5485332953383733 1103 60-61 0.6218262081548005 1103 62-63 0.4700204321168613 1103 64-65 0.42857853117164524 1103 66-67 0.37638664994641857 1103 68-69 0.6036582865687024 1103 70-71 0.8029561293895497 1103 72-73 0.544679647015684 1103 74-75 0.6022198633606024 1103 76-77 0.7304172268726496 1103 78-79 0.7041974676063489 1103 80-81 0.7231597716945437 1103 82-83 0.8112563183034425 1103 84-85 1.0022850572268247 1103 86-87 1.1268014829620547 1103 88-89 1.1061664693064834 1103 90-91 1.1499842858984195 1103 92-93 1.142537248400206 1103 94-95 1.3300973452394729 1103 96-97 1.7462523866428796 1103 98-99 1.7490388241021009 1103 100-101 2.2653953077109925 1104 1 0.3172155146040616 1104 2 0.26437375723059375 1104 3 0.23261552461947588 1104 4 -0.011990827030331275 1104 5 0.15847885313859678 1104 6 0.16013930929970144 1104 7 -0.007691306267837206 1104 8 0.15894173248450727 1104 9 0.20054755306454553 1104 10-11 0.3684059323360387 1104 12-13 0.399723820457595 1104 14-15 0.458916498636313 1104 16-17 0.6231498643293634 1104 18-19 0.5105281367414349 1104 20-21 0.4902891178089277 1104 22-23 0.426830084253929 1104 24-25 0.3981590114193878 1104 26-27 0.4968084271511515 1104 28-29 0.5013198225719222 1104 30-31 0.47763565686044984 1104 32-33 0.49137147799585534 1104 34-35 0.5887264031345083 1104 36-37 0.598070558039737 1104 38-39 0.5422893295986526 1104 40-41 0.4665575158855759 1104 42-43 0.5715206098598742 1104 44-45 0.5151263902415337 1104 46-47 0.806576817747036 1104 48-49 0.7974989607228409 1104 50-51 0.8240657216629685 1104 52-53 0.7678049163789211 1104 54-55 0.8766253789318483 1104 56-57 0.7379690402599621 1104 58-59 0.8592346780324007 1104 60-61 0.8699740672092133 1104 62-63 0.8894662394317621 1104 64-65 0.7466583706007199 1104 66-67 0.7786513689473082 1104 68-69 0.8763556549897515 1104 70-71 0.8049231231451301 1104 72-73 0.6621684962574363 1104 74-75 1.0091266430216237 1104 76-77 1.137570438291032 1104 78-79 1.0448129894618319 1104 80-81 1.1812975950665319 1104 82-83 1.3071082362338657 1104 84-85 1.5141840313570682 1104 86-87 1.706024275111922 1104 88-89 1.5660594220272692 1104 90-91 1.6510681396004685 1104 92-93 1.6623398799791538 1104 94-95 1.827969780565077 1104 96-97 2.0926897193815073 1104 98-99 2.502638288865704 1104 100-101 2.7326533362569343 1105 1 0.31379905979798295 1105 2 0.250620308861464 1105 3 0.2899758318624066 1105 4 0.3131998474977138 1105 5 0.22769073175600596 1105 6 0.44263295348210363 1105 7 0.24799322881086994 1105 8 0.41493026496679875 1105 9 0.517617926840714 1105 10-11 0.5362238584462773 1105 12-13 0.5929093021474259 1105 14-15 0.7100908373608164 1105 16-17 0.717984613071728 1105 18-19 0.5934339624567997 1105 20-21 0.6865843522980271 1105 22-23 0.8274029192190469 1105 24-25 0.708085446211399 1105 26-27 0.7478621405713923 1105 28-29 0.7199361475570498 1105 30-31 0.8150138144847219 1105 32-33 0.7306899179807118 1105 34-35 0.7255127935710348 1105 36-37 0.6183204633786232 1105 38-39 0.6957057844047299 1105 40-41 0.9057796719952691 1105 42-43 0.8816628719900237 1105 44-45 0.8764413683342482 1105 46-47 1.1046441082755152 1105 48-49 1.0501927455446065 1105 50-51 0.6750729159075703 1105 52-53 0.8646794768809016 1105 54-55 0.9828492118955481 1105 56-57 0.9039061852807961 1105 58-59 0.947440985167141 1105 60-61 1.006542466625021 1105 62-63 1.2835236852044645 1105 64-65 1.129691853588774 1105 66-67 1.1267965520489511 1105 68-69 1.2562469299397208 1105 70-71 1.3366475781675788 1105 72-73 1.3276220582199016 1105 74-75 1.4173589143474103 1105 76-77 1.4014639851077142 1105 78-79 1.340709565434139 1105 80-81 1.2925665949042546 1105 82-83 1.4964602132988283 1105 84-85 1.459773364401908 1105 86-87 1.6921642653753466 1105 88-89 1.502629444205013 1105 90-91 1.6319747226047383 1105 92-93 1.7153468578554971 1105 94-95 1.7537673680589876 1105 96-97 1.9565724477852484 1105 98-99 2.4445964216076312 1105 100-101 2.8057273882935014 1106 1 0.1796037432401718 1106 2 0.2125571188604738 1106 3 0.06233821845083298 1106 4 -0.024950645932428017 1106 5 0.07835434832818322 1106 6 0.06956771903371362 1106 7 0.17563636775593494 1106 8 0.18336731256373895 1106 9 0.1363030708913726 1106 10-11 0.15906807155504765 1106 12-13 0.0033288006500100664 1106 14-15 0.022504500900033975 1106 16-17 -0.03601255785512336 1106 18-19 0.20027346781104427 1106 20-21 0.4691855524439035 1106 22-23 0.1998012217751537 1106 24-25 0.26112448963103674 1106 26-27 0.18462959296892478 1106 28-29 0.3088806601497396 1106 30-31 0.3873187355248504 1106 32-33 0.4935389935589569 1106 34-35 0.5752685649907647 1106 36-37 0.6659030424766286 1106 38-39 0.5137720687044762 1106 40-41 0.5103039788170989 1106 42-43 0.7507226472113118 1106 44-45 0.5627098650576059 1106 46-47 0.9101817979394511 1106 48-49 0.8538373874347798 1106 50-51 0.6066463668242577 1106 52-53 0.5730793928927866 1106 54-55 0.8527770484281731 1106 56-57 0.8809967708768269 1106 58-59 0.7956070606017178 1106 60-61 0.8565275476845926 1106 62-63 1.0354990634272312 1106 64-65 1.1718649353998174 1106 66-67 1.077440276700564 1106 68-69 1.0474082865686967 1106 70-71 1.193429065420169 1106 72-73 1.1836399167441343 1106 74-75 1.3898793311936615 1106 76-77 1.3646219380080673 1106 78-79 1.1882142345099425 1106 80-81 1.3369172894638162 1106 82-83 1.0378099906198273 1106 84-85 1.0422032730095871 1106 86-87 1.1510554013145224 1106 88-89 1.4295398974092741 1106 90-91 1.4796589715189725 1106 92-93 1.536018431195906 1106 94-95 1.536243691147984 1106 96-97 1.636464478860848 1106 98-99 2.1442228955436917 1106 100-101 2.640389612431239 1107 1 0.1851318519940861 1107 2 0.24501861261229152 1107 3 0.3067665177178398 1107 4 0.36056067183695717 1107 5 0.27010416265864734 1107 6 0.2566031415530077 1107 7 0.23138566544071892 1107 8 0.1869160225640627 1107 9 0.20833639453759645 1107 10-11 0.3540679101032751 1107 12-13 0.37245760687558516 1107 14-15 0.4692415605403184 1107 16-17 0.5408691625749782 1107 18-19 0.6130959677303167 1107 20-21 0.4471054077831127 1107 22-23 0.41845697430188267 1107 24-25 0.44293694724995447 1107 26-27 0.4497608532614734 1107 28-29 0.3863775118401378 1107 30-31 0.509980106573316 1107 32-33 0.4449113336409738 1107 34-35 0.49589725820010244 1107 36-37 0.47964581750973423 1107 38-39 0.4945431623787968 1107 40-41 0.4388034460262418 1107 42-43 0.5194316788235724 1107 44-45 0.22176408056343888 1107 46-47 0.38456585942029164 1107 48-49 0.49070005655551796 1107 50-51 0.5041357917330416 1107 52-53 0.44065987660230377 1107 54-55 0.4946318064119595 1107 56-57 0.5101275145237096 1107 58-59 0.5512271968670177 1107 60-61 0.4296716595383856 1107 62-63 0.6092502339526007 1107 64-65 0.41384239673211454 1107 66-67 0.377359550813388 1107 68-69 0.5039648431252601 1107 70-71 0.7678002107590558 1107 72-73 0.8898235197019346 1107 74-75 0.69954706344204 1107 76-77 0.7686646904379195 1107 78-79 0.9758292688991617 1107 80-81 0.6372740978851432 1107 82-83 0.6357078885177252 1107 84-85 0.8642672724929419 1107 86-87 0.9011409707549305 1107 88-89 0.9938719714187663 1107 90-91 1.0853582191537043 1107 92-93 1.1175435046564637 1107 94-95 1.2710107162375905 1107 96-97 1.5680492894779157 1107 98-99 1.8227078320931405 1107 100-101 2.2962147924499696 1108 1 0.16453764401937931 1108 2 0.09105585444302733 1108 3 0.12699209702563508 1108 4 0.07448396415413328 1108 5 0.09296813561822148 1108 6 0.18911787425894744 1108 7 0.08678791281236897 1108 8 -0.08927603947886098 1108 9 0.12061195882080966 1108 10-11 0.11102946056387708 1108 12-13 0.0998264671316349 1108 14-15 0.28916808362172475 1108 16-17 0.46884876741882664 1108 18-19 0.3524754045284908 1108 20-21 0.2893391329040256 1108 22-23 0.26703739028191364 1108 24-25 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5 488.0 6 575.0 7 715.0 8 801.0 9 1058.0 10 1403.0 11 2069.0 12 2911.0 13 4045.0 14 4691.0 15 6233.0 16 8243.0 17 11019.0 18 9021.0 19 5811.0 20 5600.0 21 6037.0 22 6450.0 23 7354.0 24 8324.0 25 9709.0 26 11262.0 27 13450.0 28 15968.0 29 19636.0 30 23653.0 31 29722.0 32 37793.0 33 49165.0 34 65972.0 35 92897.0 36 135127.0 37 206438.0 38 192871.0 39 25302.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.215561815434356 22.109796362797553 15.322345747311585 22.3522960744565 2 14.106543247036013 21.924802889520407 42.09730631965319 21.871347543790385 3 17.26979951809301 26.27663337352482 32.26607675727013 24.18749035111204 4 11.147758802610497 18.864941967251553 40.92930556098324 29.057993669154712 5 11.469921326613255 39.40683802934547 36.70949268219293 12.41374796184834 6 27.77371999054774 40.32823158811594 19.139746937292866 12.758301484043457 7 25.663126671424845 34.964914113538256 22.78869089172926 16.58326832330763 8 23.667317017914932 37.07705197536868 21.63081922774207 17.62481177897432 9 24.9989502639964 17.535571403042965 23.270840677416615 34.19463765554403 10-11 22.452631656042715 28.502840513758244 30.087376009804473 18.95715182039457 12-13 23.14178953993261 26.904081952509458 29.75441296451905 20.199715543038884 14-15 20.76014469367817 28.462510051562624 28.285866196918352 22.491479057840856 16-17 19.00267159844782 31.561918395166128 29.370006620408436 20.065403385977625 18-19 18.706117106327632 30.392435920783395 32.174824126662976 18.726622846225993 20-21 19.822159953526292 29.272725497566476 32.1411590113597 18.763955537547535 22-23 19.49273540674644 29.527807540142582 31.49506866767384 19.484388385437132 24-25 19.225456132318154 29.622705426727414 32.046462088470484 19.105376352483944 26-27 19.23833626739076 29.71824261188718 32.05216080392197 18.99126031680009 28-29 18.7691370219315 29.71330415774155 32.27573033784672 19.24182848248024 30-31 19.410640902142426 29.451843114240813 32.13865308460002 18.998862899016743 32-33 19.042362040174606 29.621153677138366 31.946183217288553 19.390301065398475 34-35 19.44654653041895 29.691147966781074 31.805803059226744 19.05650244357323 36-37 19.528405365970578 29.27696275247872 31.539257978491015 19.65537390305969 38-39 18.89993448794666 30.197541784681114 31.56099308852719 19.341530638845036 40-41 19.345022502953206 29.717273091154045 31.83923812127188 19.098466284620866 42-43 19.27303581314558 29.738951372663124 31.438071739552832 19.549941074638465 44-45 19.547776913170786 29.896976164510086 31.21074486272113 19.344502059598 46-47 19.566343380160813 29.979921315771357 30.721753762983656 19.73198154108417 48-49 19.442145724062744 29.930266994948347 30.94801832401558 19.679568956973327 50-51 19.410104308620316 30.074944203213093 30.506426334813924 20.008525153352664 52-53 19.46483414146041 30.465357157333038 30.52488622817654 19.54492247303001 54-55 19.821062765706074 29.95162855731245 30.3998504137057 19.827458263275783 56-57 19.916803245483624 30.371891347361824 30.007984858054016 19.703320549100532 58-59 19.81407330472572 30.10715164246473 30.2839318415206 19.794843211288942 60-61 19.921327600403956 30.219270393786644 30.152107456794365 19.707294549015035 62-63 19.764272290156452 29.95142905957873 30.569378340777625 19.714920309487198 64-65 19.6859498404173 29.90539252365187 30.313147183617968 20.095510452312862 66-67 19.690931038640212 30.04555640759928 30.080994050283376 20.182518503477134 68-69 19.72509017138054 29.789382890453425 30.252675120899696 20.232851817266337 70-71 19.680201725069544 29.873887664010223 30.65271199851225 19.793198612407988 72-73 19.925151878134333 29.509216169224896 30.53734059263468 20.028291360006094 74-75 19.578168725675923 30.0565887889472 30.17813289701114 20.18710958836574 76-77 19.534667777808696 29.92531036061831 30.392224349144648 20.14779751242834 78-79 19.361554205008826 29.81768595622354 30.338429781117693 20.482330057649946 80-81 19.405650392692138 29.796907280914496 30.23646471628921 20.56097761010416 82-83 19.532082552876986 30.066961812191572 30.21219137497492 20.188764259956525 84-85 19.67408208671814 29.664739184638982 30.09859348808511 20.562585240557762 86-87 19.40492601603474 29.825847720731524 30.278557732486167 20.490668530747573 88-89 19.975415474094227 29.45871571725452 30.095345586867683 20.47052322178357 90-91 19.635735936135408 29.327655569381665 30.57600859963514 20.460599894847785 92-93 19.810087467940303 29.363365392219364 30.61539002266024 20.211157117180093 94-95 19.727672312456797 29.257042267272254 30.499714911681203 20.51557050858975 96-97 19.934263125166563 29.081148042683086 30.186199161059246 20.7983896710911 98-99 19.67886424121011 29.089516568355815 30.12602728492183 21.10559190551224 100-101 19.89804684144351 28.65919491757062 30.872068505025418 20.570689735960453 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1880.0 1 1100.5 2 325.0 3 335.5 4 296.5 5 301.5 6 495.5 7 897.0 8 1333.0 9 1484.5 10 1253.5 11 1011.0 12 950.5 13 992.0 14 1114.5 15 1154.5 16 1198.0 17 1295.5 18 1435.0 19 1661.0 20 2024.0 21 2448.0 22 2903.0 23 3551.0 24 4472.5 25 6169.0 26 9290.0 27 12837.0 28 14462.5 29 16561.0 30 20778.5 31 25021.5 32 29918.5 33 35498.0 34 41705.0 35 46362.0 36 49219.5 37 51307.5 38 49037.5 39 45833.5 40 44771.0 41 43487.5 42 41467.5 43 40411.0 44 39701.0 45 37777.5 46 35793.5 47 32942.5 48 31892.0 49 30154.5 50 25996.0 51 22560.5 52 19518.5 53 17110.5 54 14952.5 55 12794.5 56 10580.0 57 8616.0 58 6918.5 59 5408.0 60 4215.0 61 3211.0 62 2269.5 63 1510.0 64 966.0 65 643.5 66 421.0 67 295.0 68 228.0 69 167.0 70 117.5 71 85.5 72 73.5 73 71.0 74 56.0 75 34.5 76 23.0 77 22.5 78 17.0 79 15.0 80 14.5 81 6.0 82 2.5 83 3.0 84 1.5 85 0.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.312578558747815 2 0.29406548949529476 3 0.279449908506463 4 0.26717282047584434 5 0.2633727694187481 6 0.21514135215560332 7 0.20471557105023666 8 0.2182593427665541 9 0.21777215673359304 10-11 0.22210811242694647 12-13 0.21879524740281128 14-15 0.2150926335523072 16-17 0.2139721056764968 18-19 0.21421569869297732 20-21 0.20140270602610147 22-23 0.19336413648224401 24-25 0.1932179806723557 26-27 0.20685918959526534 28-29 0.22142605198080095 30-31 0.214995196345715 32-33 0.2213773333775049 34-35 0.21309517081716686 36-37 0.19804112239867017 38-39 0.20101295719973264 40-41 0.19409491553168562 42-43 0.20705406400844975 44-45 0.20198732926565477 46-47 0.20281554552168857 48-49 0.2125592661809097 50-51 0.22152348918739317 52-53 0.23701600503555484 54-55 0.2089053709337018 56-57 0.1815255158812903 58-59 0.18181782750106693 60-61 0.18781021570648795 62-63 0.197505217762413 64-65 0.18625122040101255 66-67 0.19170770397017642 68-69 0.19511800620090383 70-71 0.18878458777241006 72-73 0.19107436212732704 74-75 0.1931692620690596 76-77 0.20758996864470694 78-79 0.21338748243694353 80-81 0.20364376177772234 82-83 0.2008668013898443 84-85 0.19438722715146223 86-87 0.1974077805558208 88-89 0.20271810831509635 90-91 0.20193861066235863 92-93 0.19886933865470396 94-95 0.2005257711667716 96-97 0.18659225062408533 98-99 0.19487441318442328 100-101 0.18610506459112425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026302.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.492900552408486 #Duplication Level Percentage of deduplicated Percentage of total 1 79.01184973129637 44.636085693319686 2 13.023750071528713 14.714988352205891 3 3.841704950136516 6.510871670992728 4 1.4944962573984522 3.3771371378062978 5 0.7174461757917481 2.02653077303545 6 0.40211346903662554 1.3629933730242043 7 0.2445351278144358 0.9670149060033992 8 0.17179799148864047 0.7764293478617028 9 0.12535497785698146 0.6373499668041439 >10 0.7627999483977659 8.779975786283337 >50 0.11879454168302143 4.71878623135566 >100 0.08016263472720345 8.37863096926161 >500 0.0043284357029822265 1.6594504843963322 >1k 6.925497124771563E-4 0.9415224780619531 >5k 1.7313742811928906E-4 0.5122328295876218 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5237 0.5102786509234124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.39695917965667027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2821 0.27487035979662905 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1465 0.14274550765759006 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1266 0.12335550354573994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.359046362571641E-4 0.0 24-25 0.0 0.0 0.0 0.0011205278758104338 0.0 26-27 0.0 0.0 0.0 0.0014615580988831746 0.0 28-29 0.0 0.0 0.0 0.0029718348010624555 0.0 30-31 0.0 0.0 0.0 0.0063334184284937565 0.0 32-33 0.0 0.0 0.0 0.012520681047099197 0.0 34-35 0.0 0.0 0.0 0.019097692492073482 0.0 36-37 0.0 0.0 0.0 0.025869578350232195 0.0 38-39 0.0 0.0 0.0 0.03166709214246878 0.0 40-41 0.0 0.0 0.0 0.03624664085230273 0.0 42-43 0.0 0.0 0.0 0.041556968611578274 0.0 44-45 0.0 0.0 0.0 0.04828013586644087 0.0 46-47 0.0 0.0 0.0 0.057244358872924346 0.0 48-49 0.0 0.0 0.0 0.0678162957881793 0.0 50-51 0.0 0.0 0.0 0.08165237912427337 0.0 52-53 0.0 0.0 0.0 0.10450140407014699 0.0 54-55 0.0 0.0 0.0 0.14596093547513306 0.0 56-57 0.0 0.0 0.0 0.223277358906053 0.0 58-59 0.0 0.0 0.0 0.3116041866818928 0.0 60-61 0.0 0.0 0.0 0.3907231984347687 0.0 62-63 0.0 0.0 0.0 0.49074249100167394 0.0 64-65 0.0 0.0 0.0 0.6121005318122736 0.0 66-67 0.0 0.0 0.0 0.7983517522132861 0.0 68-69 0.0 0.0 0.0 1.0071109673370997 0.0 70-71 0.0 0.0 0.0 1.1856159298140314 0.0 72-73 0.0 0.0 0.0 1.366849134075545 0.0 74-75 0.0 0.0 0.0 1.577751967744387 0.0 76-77 0.0 0.0 0.0 1.8379580279488885 0.0 78-79 0.0 0.0 0.0 2.064353377465892 0.0 80-81 0.0 0.0 0.0 2.2970821454113897 0.0 82-83 0.0 0.0 0.0 2.544572650155607 0.0 84-85 0.0 0.0 0.0 2.87093857363622 0.0 86-87 0.0 0.0 0.0 3.254353981576573 0.0 88-89 0.0 0.0 0.0 3.7075344294369494 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4765 0.0 36.115734 1 GGTATCA 2900 0.0 34.42485 1 TCAACGC 5740 0.0 29.388426 4 CTCGGTA 65 0.0058439993 29.230543 9 CAACGCA 5790 0.0 29.051153 5 ATCAACG 5895 0.0 28.856117 3 TATCAAC 5910 0.0 28.630468 2 AACGCAG 5930 0.0 28.2755 6 CATTCCG 355 0.0 26.760355 9 GTACATG 4070 0.0 26.514427 1 ACGCAGA 6560 0.0 25.487608 7 CGCAGAG 6555 0.0 25.434586 8 ACATGGG 4060 0.0 25.396414 3 TACATGG 4190 0.0 25.296288 2 AGTACTT 3910 0.0 23.689077 12-13 GCAGAGT 6870 0.0 23.369543 9 CATGGGG 1485 0.0 23.039124 4 GAGTACT 3885 0.0 22.863401 12-13 CGCGGCA 55 0.002878256 21.590742 36-37 AGAGTAC 6305 0.0 20.83002 10-11 >>END_MODULE