FastQCFastQC Report
Thu 26 May 2016
SRR937649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026302
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59800.5826744954214256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50030.48747834458083494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.34346615323754603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11740.11439128053925647No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT11530.11234509920082004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.10883735976350041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38900.035.5542071
CATTCCG3150.034.687619
GTATCAA58700.029.8769251
TCAACGC69000.025.2644924
CAACGCA69800.024.9749285
ATCAACG69900.024.8712463
AACGCAG70300.024.796096
TATCAAC71700.024.3131122
GTACATG37850.022.7279511
ACCGTGC2103.439891E-822.6223557
TTAGGCA5800.022.1120684
CCGTGCA2154.413596E-822.0962528
GAGTACT47750.022.08712-13
ACGCAGA78350.022.0102357
CGCAGAG78550.021.8332358
TACATGG38600.021.6580332
TAGGCAT6000.021.3755
AGTACTT48850.020.95751612-13
ACATGGG39700.020.818643
GTGTAGC6050.020.4251651
Produced by FastQC (version 0.11.5)