##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937649_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026302 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14983309006511 33.0 31.0 34.0 31.0 34.0 2 32.36141116357563 34.0 31.0 34.0 31.0 34.0 3 32.42552289676918 34.0 31.0 34.0 31.0 34.0 4 36.055349205204706 37.0 35.0 37.0 35.0 37.0 5 35.97265619671403 37.0 35.0 37.0 35.0 37.0 6 36.01286463438637 37.0 35.0 37.0 35.0 37.0 7 35.97156295125606 37.0 35.0 37.0 35.0 37.0 8 35.93613673168327 37.0 35.0 37.0 35.0 37.0 9 37.6091832618469 39.0 37.0 39.0 35.0 39.0 10-11 37.54155696861158 39.0 37.0 39.0 35.0 39.0 12-13 37.458082026537994 39.0 37.0 39.0 34.5 39.0 14-15 38.674350727173874 40.0 38.0 41.0 34.0 41.0 16-17 38.62844464884605 40.0 38.0 41.0 34.0 41.0 18-19 38.645635008019084 40.0 38.0 41.0 34.0 41.0 20-21 38.57011289074756 40.0 38.0 41.0 34.0 41.0 22-23 38.5210269491826 40.0 38.0 41.0 34.0 41.0 24-25 38.54486350021728 40.0 38.0 41.0 34.0 41.0 26-27 38.37943071337676 40.0 38.0 41.0 34.0 41.0 28-29 38.34494817314982 40.0 38.0 41.0 34.0 41.0 30-31 38.31528097967265 40.0 38.0 41.0 34.0 41.0 32-33 38.18057842623321 40.0 37.5 41.0 34.0 41.0 34-35 38.046337237966995 40.0 37.0 41.0 33.5 41.0 36-37 37.872747982562636 40.0 37.0 41.0 33.0 41.0 38-39 37.74475398079707 40.0 37.0 41.0 33.0 41.0 40-41 37.59507922619268 40.0 37.0 41.0 33.0 41.0 42-43 37.373607378724785 40.0 37.0 41.0 33.0 41.0 44-45 37.05632991068906 40.0 37.0 41.0 31.5 41.0 46-47 36.71740481846474 40.0 36.5 41.0 31.0 41.0 48-49 36.392681686287276 40.0 36.0 41.0 30.0 41.0 50-51 36.19397896525584 39.0 36.0 41.0 30.0 41.0 52-53 35.976638455347455 39.0 36.0 41.0 29.0 41.0 54-55 35.78333716586346 39.0 35.5 40.0 28.5 41.0 56-57 35.54954292206388 39.0 35.0 40.0 28.0 41.0 58-59 35.35150228685124 39.0 35.0 40.0 27.5 41.0 60-61 35.074877570149916 38.0 35.0 40.0 26.5 41.0 62-63 34.712873988358204 38.0 34.0 40.0 26.0 41.0 64-65 34.364127225709396 38.0 34.0 40.0 26.0 41.0 66-67 33.983342135160996 37.0 34.0 40.0 25.0 41.0 68-69 33.53234622947242 37.0 33.0 39.0 23.0 40.5 70-71 33.13314307094792 36.0 32.5 39.0 22.5 40.0 72-73 32.6697882299752 36.0 32.0 38.5 22.0 40.0 74-75 32.17569682218294 35.0 31.5 38.0 20.5 39.5 76-77 30.809450824416206 34.0 30.0 36.0 20.0 39.0 78-79 31.425757720437066 35.0 31.0 37.0 20.0 39.0 80-81 31.235121825739405 35.0 31.0 36.0 18.5 38.5 82-83 30.81657592014826 34.0 31.0 36.0 17.5 37.5 84-85 30.37199479295568 34.0 30.5 35.5 12.0 37.0 86-87 30.01386823761427 34.0 30.0 35.0 7.5 36.5 88-89 29.57999204912394 34.0 30.0 35.0 4.5 36.0 90-91 29.08146286375745 34.0 29.0 35.0 2.0 36.0 92-93 28.64591562717407 34.0 29.0 35.0 2.0 35.0 94-95 28.166787164012153 34.0 29.0 35.0 2.0 35.0 96-97 27.65632874144258 33.0 27.5 35.0 2.0 35.0 98-99 26.865863556730865 33.0 26.5 34.5 2.0 35.0 100-101 24.409542707702023 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44324098191976447 1101 2 0.3829042996670182 1101 3 0.33354738091956193 1101 4 0.23187813367439247 1101 5 0.2777490055756999 1101 6 0.26185900213187097 1101 7 0.3191321808052976 1101 8 0.3397926263960329 1101 9 0.3133638262179588 1101 10-11 0.3920623827751655 1101 12-13 0.43763006503951374 1101 14-15 0.4984781443131965 1101 16-17 0.539454008579888 1101 18-19 0.6676399476258013 1101 20-21 0.6095983078833171 1101 22-23 0.6430371826352896 1101 24-25 0.5406144705924163 1101 26-27 0.5551642166891142 1101 28-29 0.45864775801052104 1101 30-31 0.5361978942343413 1101 32-33 0.5933761324200617 1101 34-35 0.5249813736323787 1101 36-37 0.4040199601116896 1101 38-39 0.5414639846817124 1101 40-41 0.6594328701563725 1101 42-43 0.6817041982869299 1101 44-45 0.5788942037300089 1101 46-47 0.6143938434439065 1101 48-49 0.4792056814734025 1101 50-51 0.3365256008672546 1101 52-53 0.42360648358154407 1101 54-55 0.5399010911637703 1101 56-57 0.4602785921443555 1101 58-59 0.5032379853070381 1101 60-61 0.6400286131155397 1101 62-63 0.430644396436513 1101 64-65 0.650643247746963 1101 66-67 0.8100807720627472 1101 68-69 0.8364129840688435 1101 70-71 0.7043480750257345 1101 72-73 0.44720308289480215 1101 74-75 0.6271023344769802 1101 76-77 0.7202617980721158 1101 78-79 0.7816514699962305 1101 80-81 0.7921215780935462 1101 82-83 0.8031296036502091 1101 84-85 0.8559816732230914 1101 86-87 0.9417366592325749 1101 88-89 1.05999937552372 1101 90-91 1.0065706279421818 1101 92-93 1.2949957211188448 1101 94-95 1.5102564818699555 1101 96-97 1.4549119696589656 1101 98-99 1.3523736180387722 1101 100-101 2.22957848539998 1102 1 0.23591514402038882 1102 2 0.17497479469773936 1102 3 0.20063981669839848 1102 4 0.15554686395736894 1102 5 0.09778150160264687 1102 6 0.15336496764338392 1102 7 0.22056248038546045 1102 8 0.2928477273234691 1102 9 0.27615113141969516 1102 10-11 0.27151398267080395 1102 12-13 0.3091391141156308 1102 14-15 0.25870609089636787 1102 16-17 0.17600077202303765 1102 18-19 0.34289730667131835 1102 20-21 0.4077671449050726 1102 22-23 0.36491981742263846 1102 24-25 0.29134741017827537 1102 26-27 0.21941265711701874 1102 28-29 0.42336525605364983 1102 30-31 0.3088834418042481 1102 32-33 0.3162069633221236 1102 34-35 0.2666717600267461 1102 36-37 0.4002912189176371 1102 38-39 0.24890189829446996 1102 40-41 0.37022238129279117 1102 42-43 0.3711246067406435 1102 44-45 0.49123854301699765 1102 46-47 0.5787069201297825 1102 48-49 0.6967363392214523 1102 50-51 0.5254722077602096 1102 52-53 0.51776194267687 1102 54-55 0.4386427596094151 1102 56-57 0.5065842445554622 1102 58-59 0.4376072777475599 1102 60-61 0.36355087407998354 1102 62-63 0.4002351125350927 1102 64-65 0.5896734666800469 1102 66-67 0.5491530935124871 1102 68-69 0.6772346354362924 1102 70-71 0.5520442964731131 1102 72-73 0.7011423366721701 1102 74-75 0.5926102924456202 1102 76-77 0.38909763383672313 1102 78-79 0.5985612638717974 1102 80-81 0.6572120688547152 1102 82-83 0.5109488118857008 1102 84-85 0.6739991783821324 1102 86-87 0.5700953726745475 1102 88-89 0.5080585088048686 1102 90-91 0.7295455561900042 1102 92-93 1.041552209650355 1102 94-95 1.2419685718151605 1102 96-97 1.271282139729415 1102 98-99 1.5280057487819114 1102 100-101 2.1050350901581574 1103 1 0.2501325980413043 1103 2 0.20867475863570917 1103 3 0.14711024583651522 1103 4 0.18109680986432863 1103 5 0.0815535895939874 1103 6 0.18230474405881836 1103 7 0.17100424021236194 1103 8 0.22193625959898355 1103 9 0.20775048230321147 1103 10-11 0.13457745183777092 1103 12-13 0.2740823164235948 1103 14-15 0.36911449334859014 1103 16-17 0.3213848326072437 1103 18-19 0.46156165935794036 1103 20-21 0.35643510379436094 1103 22-23 0.43898896635881357 1103 24-25 0.36618062330485657 1103 26-27 0.28775921319346764 1103 28-29 0.2626908961546164 1103 30-31 0.38510278908156437 1103 32-33 0.425736353873873 1103 34-35 0.3802085432939677 1103 36-37 0.31601651642935735 1103 38-39 0.34376756724506663 1103 40-41 0.41433976318965193 1103 42-43 0.45959377370782306 1103 44-45 0.48996961045298804 1103 46-47 0.47736811738906226 1103 48-49 0.5224778646451753 1103 50-51 0.5123523916765436 1103 52-53 0.5225739693627673 1103 54-55 0.46319649202917645 1103 56-57 0.5504018608555086 1103 58-59 0.5243612445704926 1103 60-61 0.6054211409389794 1103 62-63 0.3929280443778609 1103 64-65 0.5798375489014731 1103 66-67 0.9023333134908569 1103 68-69 0.5545471128975237 1103 70-71 0.74038273859356 1103 72-73 0.8065358635383788 1103 74-75 0.8763913959436351 1103 76-77 0.8040385822680243 1103 78-79 0.8209467669370731 1103 80-81 0.882279685154753 1103 82-83 0.8358811955856638 1103 84-85 0.9587855108740193 1103 86-87 0.9860776121264045 1103 88-89 0.9912851586425866 1103 90-91 0.9210619644121465 1103 92-93 0.9465986395097126 1103 94-95 1.03529935075494 1103 96-97 1.370504552278998 1103 98-99 1.5659114465821276 1103 100-101 2.100099100255523 1104 1 0.20860271400918862 1104 2 0.2039557577436497 1104 3 0.23713477750466438 1104 4 0.1721762032630778 1104 5 0.18746795177061415 1104 6 0.21157325431752128 1104 7 0.22692306269229334 1104 8 0.1377413443447466 1104 9 0.22886109782506736 1104 10-11 0.18817691660137115 1104 12-13 0.2588927535333241 1104 14-15 0.33351012225135435 1104 16-17 0.4032180172638036 1104 18-19 0.3713687512401833 1104 20-21 0.278931758566884 1104 22-23 0.34708107162197166 1104 24-25 0.3561962343664078 1104 26-27 0.42709462800167586 1104 28-29 0.2871110567255357 1104 30-31 0.3000704518826396 1104 32-33 0.47698523879804355 1104 34-35 0.40951050582742 1104 36-37 0.3022509053678064 1104 38-39 0.39167345484541727 1104 40-41 0.35060871947867867 1104 42-43 0.47011116889069626 1104 44-45 0.6186125631737767 1104 46-47 0.8688021049002117 1104 48-49 0.8864731813267142 1104 50-51 0.838932454120787 1104 52-53 0.8136633984439499 1104 54-55 0.7454288738311377 1104 56-57 0.8609627439955574 1104 58-59 0.7862802900655907 1104 60-61 0.7496706949086516 1104 62-63 0.8460301853234498 1104 64-65 0.9239231867248421 1104 66-67 1.0788955347218945 1104 68-69 0.9914003689189315 1104 70-71 1.0038640053196985 1104 72-73 1.0013830981503347 1104 74-75 1.073371770609107 1104 76-77 1.1148983057292199 1104 78-79 1.0336452504339455 1104 80-81 0.9310642525053332 1104 82-83 0.8792018913929773 1104 84-85 0.8072578569935551 1104 86-87 0.809272304365475 1104 88-89 0.9508636599806799 1104 90-91 1.0705757913523826 1104 92-93 0.8898780775114972 1104 94-95 0.9498745068655552 1104 96-97 0.9690872909052288 1104 98-99 1.3819083243698174 1104 100-101 1.8730362099789843 1105 1 0.21122401546426772 1105 2 0.2694818030728001 1105 3 0.1637556462232297 1105 4 0.11416246387864248 1105 5 0.16085141041723716 1105 6 0.21747902594081836 1105 7 0.20672941310068893 1105 8 0.21807022409808496 1105 9 0.38257019070114495 1105 10-11 0.28388633402983743 1105 12-13 0.3420041290945264 1105 14-15 0.26627098123334036 1105 16-17 0.5193985765757532 1105 18-19 0.4931110566439827 1105 20-21 0.42251643969416364 1105 22-23 0.40189364261780725 1105 24-25 0.28189321061998385 1105 26-27 0.3170827277474473 1105 28-29 0.28800357579299174 1105 30-31 0.30577067366009913 1105 32-33 0.3106981181186512 1105 34-35 0.32792506741123617 1105 36-37 0.3389280298336459 1105 38-39 0.34544745819913203 1105 40-41 0.3812307782226654 1105 42-43 0.3715078260010998 1105 44-45 0.2906913212199669 1105 46-47 0.17418318349598394 1105 48-49 0.19301074826564246 1105 50-51 0.14746385456434297 1105 52-53 0.10805080581128124 1105 54-55 0.19067201766194586 1105 56-57 0.24746461017178945 1105 58-59 0.22731939862836725 1105 60-61 0.20341278394418794 1105 62-63 0.27096229978225495 1105 64-65 -0.04159799031848621 1105 66-67 0.12951542978329655 1105 68-69 0.13022494835313125 1105 70-71 0.11849320421622167 1105 72-73 0.18372690731352748 1105 74-75 0.2142312935170949 1105 76-77 0.1290103844872661 1105 78-79 0.21463351245591156 1105 80-81 0.3368899678728816 1105 82-83 0.3486398748408064 1105 84-85 0.4213434306399009 1105 86-87 0.3608658565913636 1105 88-89 0.420950748204838 1105 90-91 0.54098418644082 1105 92-93 0.8074839627281349 1105 94-95 0.9124054745725907 1105 96-97 0.7942720153061558 1105 98-99 0.9851841398076928 1105 100-101 1.1206399315130362 1106 1 -0.29424957801118623 1106 2 -0.3040917804566874 1106 3 -0.33018836907145044 1106 4 -0.20230031060223297 1106 5 -0.18953261416034195 1106 6 -0.25448220691620804 1106 7 -0.17303166283119253 1106 8 -0.1594629567305219 1106 9 -0.5938214149083763 1106 10-11 -0.5024965412367663 1106 12-13 -0.5648027756121223 1106 14-15 -0.5074915755415219 1106 16-17 -0.38889856451321947 1106 18-19 -0.518982125952796 1106 20-21 -0.6676160625989311 1106 22-23 -0.6044922164368884 1106 24-25 -0.4136056897988425 1106 26-27 -0.5206925819587056 1106 28-29 -0.6180955080735586 1106 30-31 -0.6138344717166788 1106 32-33 -0.8340504148522072 1106 34-35 -0.9410667437481308 1106 36-37 -0.8726783532626499 1106 38-39 -0.9103808745263962 1106 40-41 -0.9333980716927996 1106 42-43 -1.1278401610470468 1106 44-45 -0.9334983592936865 1106 46-47 -0.9203150428077578 1106 48-49 -0.8239625855097259 1106 50-51 -1.177524818092003 1106 52-53 -1.201707286332514 1106 54-55 -1.4849559592192279 1106 56-57 -1.696275512937099 1106 58-59 -1.6416823584912876 1106 60-61 -1.6163564697772586 1106 62-63 -1.643856628485267 1106 64-65 -1.924345064548504 1106 66-67 -1.8806913356799129 1106 68-69 -1.8985260600567386 1106 70-71 -1.736373685681997 1106 72-73 -2.1517917745152033 1106 74-75 -1.9603180992267681 1106 76-77 -1.832419181537336 1106 78-79 -1.7082449589605027 1106 80-81 -1.830056290788388 1106 82-83 -2.1410301402991543 1106 84-85 -1.9728779664359841 1106 86-87 -1.8086337510957584 1106 88-89 -2.2141618069123616 1106 90-91 -2.433872425965106 1106 92-93 -2.245447332788448 1106 94-95 -1.990300931730939 1106 96-97 -1.9694865630845868 1106 98-99 -2.079206559614338 1106 100-101 -1.4655036881534436 1107 1 0.14453759625985896 1107 2 0.21074886664202097 1107 3 0.13522866086170637 1107 4 0.10350226616808555 1107 5 0.08506133462715582 1107 6 0.06423328276702023 1107 7 0.1199128946294934 1107 8 0.020704307307710224 1107 9 0.1433576996900925 1107 10-11 0.14268089426325048 1107 12-13 0.16486398792560664 1107 14-15 0.15442840895911303 1107 16-17 0.2189964272527405 1107 18-19 0.2005373935667265 1107 20-21 0.06004708441905393 1107 22-23 0.04581454193439072 1107 24-25 0.0010347570996600552 1107 26-27 0.09569638765606925 1107 28-29 0.08767477834189208 1107 30-31 0.19367142127308057 1107 32-33 0.40125424464600457 1107 34-35 0.16007633481429906 1107 36-37 0.11544041066257194 1107 38-39 0.21214972058484705 1107 40-41 0.3128304148582757 1107 42-43 0.4596828458307982 1107 44-45 0.6089376250777789 1107 46-47 0.22768941326120995 1107 48-49 0.3510156185007318 1107 50-51 0.34773915555906854 1107 52-53 0.3572290693780431 1107 54-55 0.3709612483108842 1107 56-57 0.30787702669930894 1107 58-59 0.28967157608845184 1107 60-61 0.30779671566624955 1107 62-63 0.2417079683696528 1107 64-65 0.35811158565008583 1107 66-67 0.5957560794771837 1107 68-69 0.5840904273984648 1107 70-71 0.6451869071689202 1107 72-73 0.6398304929135108 1107 74-75 0.5657014687282853 1107 76-77 0.4384097118722039 1107 78-79 0.5520104577465261 1107 80-81 0.6820784245721292 1107 82-83 0.6484448185306455 1107 84-85 0.7682082811018773 1107 86-87 0.8667787581877207 1107 88-89 0.8179412639003907 1107 90-91 0.7682587374563781 1107 92-93 0.8039310778802857 1107 94-95 0.8200093785793747 1107 96-97 1.0084671971272385 1107 98-99 1.0276793585619046 1107 100-101 1.538114446109855 1108 1 0.16532607115923525 1108 2 0.09757354983381816 1108 3 0.09825382751976264 1108 4 0.13323084752190795 1108 5 -0.03171892555654665 1108 6 0.01756902768976687 1108 7 0.08695929093018862 1108 8 0.08164191795851394 1108 9 0.11208742367794144 1108 10-11 0.16003763915345104 1108 12-13 0.22885149359571955 1108 14-15 0.281628266560503 1108 16-17 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-0.21644674835064848 2316 68-69 -0.2732200080369722 2316 70-71 -0.16140093066891836 2316 72-73 -0.1105292418212187 2316 74-75 -0.11283644418463012 2316 76-77 -0.12360542826793619 2316 78-79 -0.2622100127239442 2316 80-81 -0.25808048433677655 2316 82-83 -0.35906268897686644 2316 84-85 -0.4846695967760013 2316 86-87 -0.21966706263310343 2316 88-89 0.00267287050699494 2316 90-91 -0.20391656284392212 2316 92-93 -0.10313223543302996 2316 94-95 -0.24100476055976472 2316 96-97 -0.06222849856845869 2316 98-99 -0.07734754334000016 2316 100-101 0.43465160827201643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 5.0 5 27.0 6 51.0 7 111.0 8 219.0 9 457.0 10 815.0 11 1481.0 12 2380.0 13 3408.0 14 4290.0 15 6179.0 16 8381.0 17 11712.0 18 10051.0 19 5893.0 20 4706.0 21 4579.0 22 4877.0 23 5369.0 24 6272.0 25 7350.0 26 8905.0 27 11088.0 28 13986.0 29 17875.0 30 22221.0 31 27914.0 32 35660.0 33 46679.0 34 62776.0 35 88481.0 36 135305.0 37 229585.0 38 217580.0 39 19623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.444720093657914 22.623630296892074 15.962396465018166 21.969253144431846 2 14.367505860847976 22.52465648512816 41.40886405755811 21.698973596465756 3 17.434244501131246 26.454299026992057 32.567314494174234 23.54414197770247 4 11.48785785066954 19.38593063828253 40.60816466124461 28.518046849803326 5 11.46979146365231 39.45517442993569 36.70259208731241 12.37244201909959 6 28.011735337161962 40.49587743178908 18.729867037187883 12.762520193861066 7 25.594610553228968 35.03023476520556 22.736387535053034 16.63876714651243 8 23.848048625063576 37.25764930790352 21.397405442062862 17.496896624970038 9 24.96009946390049 17.310206937139363 23.14377249581507 34.585921103145076 10-11 22.576639234845103 28.475682596350783 29.99351068204096 18.954167486763154 12-13 23.352238760884912 26.761013536195183 29.90381959145373 19.98292811146618 14-15 20.83007730667971 28.41200738184277 28.405479089001094 22.352436222476424 16-17 18.961134247034497 31.540375055295616 29.60965680667094 19.888833890998946 18-19 18.633940107297853 30.036236897131648 32.78679180202319 18.543031193547318 20-21 19.637756461692188 28.990201193902166 32.81491690770278 18.55712543670287 22-23 19.404700425805572 29.155550575373436 32.17282639409913 19.266922604721863 24-25 19.140613581577355 29.228287580069022 32.765453053779495 18.86564578457413 26-27 19.197402300026553 29.372230626259082 32.76760767519981 18.662759398514556 28-29 18.74845316625905 29.373081682565356 32.93065312923248 18.94781202194312 30-31 19.371344886787707 29.047736436253658 32.83180779146878 18.749110885489845 32-33 19.091992415487837 29.288016587710054 32.564245222166576 19.055745774635536 34-35 19.48870800212803 29.307211717408716 32.54310134833606 18.66097893212719 36-37 19.569510999729122 28.93253226645672 32.21715330245211 19.28080343136204 38-39 18.93745700583259 29.713232557278463 32.289277425163355 19.060033011725594 40-41 19.393735070771182 29.313636698023632 32.53637701628324 18.756251214921946 42-43 19.291512584281378 29.311476496233556 32.13853600252997 19.258474916955095 44-45 19.57766817174672 29.406841261149253 31.87404876927064 19.141441797833387 46-47 19.676177187611444 29.705876048180752 31.185703623299965 19.432243140907843 48-49 19.592624782958623 29.61525944604999 31.327279884478447 19.464835886512937 50-51 19.554721709594254 29.74611761450333 30.94552090905016 19.753639766852253 52-53 19.676372062024626 30.212257210840477 30.72448460589573 19.386886121239165 54-55 20.104170117567733 29.71474283398064 30.609313827703737 19.57177322074789 56-57 19.98422491625272 30.148484559125873 30.28012222523195 19.58716829938946 58-59 19.91017263924264 29.91731478648585 30.52673579511684 19.645776779154676 60-61 20.082149308877895 30.008516011856155 30.421795923617022 19.487538755648924 62-63 19.960304082034334 29.752743344551604 30.776808385835746 19.510144187578316 64-65 20.01223811314798 29.614918415826917 30.519525441829014 19.853318029196085 66-67 19.754711576124766 29.9192148120144 30.31252009642386 20.01355351543698 68-69 19.85224621992357 29.5300993274884 30.493363551859005 20.124290900729026 70-71 19.902475099921855 29.663052395883472 30.817975605620955 19.616496898573715 72-73 20.17529927838005 29.29337563407262 30.68321994890393 19.8481051386434 74-75 19.868225921804694 29.829572581949567 30.21898037809534 20.08322111815041 76-77 19.682023420006978 29.711429968956505 30.585831460915013 20.020715150121504 78-79 19.480230965154508 29.647413724225423 30.512168932731303 20.36018637788877 80-81 19.566998797624873 29.61866974828072 30.411321423908362 20.403010030186046 82-83 19.559007872458285 29.883040964162994 30.427570119638563 20.130381043740154 84-85 19.55848149515882 29.656269761541225 30.2485435530971 20.536705190202856 86-87 19.40885821132571 29.599864367408422 30.372882445907734 20.618394975358132 88-89 19.904959748689958 29.264972688350994 30.3295716075775 20.500495955381552 90-91 19.508994826549575 29.142633850483485 30.757765778828905 20.59060554413804 92-93 19.587772513880257 29.144150025626587 30.870241786338593 20.397835674154567 94-95 19.523541803484747 29.017871932433142 30.707871562171757 20.750714701910354 96-97 19.910854699688784 28.79756640832815 30.37892355271645 20.91265533926661 98-99 19.526248711387918 28.897893204507852 30.207015895967835 21.368842188136387 100-101 19.327163185706176 28.832731179547398 30.808510223621155 21.031595411125267 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 706.0 1 452.5 2 277.0 3 344.0 4 267.5 5 253.5 6 442.0 7 894.5 8 1348.0 9 1510.5 10 1263.5 11 951.5 12 962.5 13 1019.0 14 1027.5 15 1077.0 16 1198.5 17 1391.5 18 1550.0 19 1768.0 20 2136.0 21 2617.0 22 3199.0 23 3845.5 24 4707.5 25 6337.5 26 9615.0 27 13317.5 28 14824.0 29 16991.0 30 20923.0 31 24736.0 32 29953.0 33 35588.5 34 41355.0 35 45743.0 36 48623.5 37 51103.5 38 49183.0 39 45838.5 40 44736.5 41 43795.5 42 41783.0 43 40576.5 44 39932.5 45 38080.5 46 35851.0 47 33138.5 48 32220.5 49 30079.5 50 25969.5 51 22751.0 52 19640.0 53 17158.5 54 14836.5 55 12477.0 56 10422.0 57 8568.0 58 6723.0 59 5161.0 60 3965.0 61 2983.0 62 2197.0 63 1474.0 64 872.5 65 546.5 66 346.0 67 241.0 68 190.5 69 159.5 70 124.5 71 79.0 72 61.0 73 47.5 74 30.5 75 28.5 76 20.5 77 10.5 78 10.0 79 10.0 80 8.5 81 5.0 82 1.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04296980810716534 2 0.0 3 0.0 4 9.743720659221164E-5 5 0.009938595072405588 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005212890552683323 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.4103022307274076E-4 22-23 0.0011692464791065397 24-25 0.0 26-27 9.256534626260107E-4 28-29 0.0011692464791065397 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.8974882636884654E-4 38-39 0.0 40-41 1.4615580988831746E-4 42-43 0.019536159921738436 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.435930164805291E-4 84-85 2.923116197766349E-4 86-87 0.0 88-89 0.0 90-91 0.009841157865813377 92-93 0.0023384929582130794 94-95 0.0 96-97 0.0 98-99 3.8974882636884654E-4 100-101 0.012130932220730351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026302.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.5525107173159 #Duplication Level Percentage of deduplicated Percentage of total 1 78.27335811302063 43.48281565453884 2 13.397267434753918 14.885036855038283 3 4.011030580763372 6.684684579760171 4 1.5850816362857294 3.522210583503344 5 0.77973710555136 2.16581769564154 6 0.41502154697655436 1.3833293361799235 7 0.2564384644340477 0.9972060380663126 8 0.16901235315746962 0.7511248448111287 9 0.11671395927234678 0.5835378126003673 >10 0.7925822108853056 9.056660819255235 >50 0.11679299789443932 4.609670100762647 >100 0.08184809155493179 8.564082563618564 >500 0.0038807282724106227 1.4557812997787638 >1k 8.819836982751415E-4 0.7817866996254025 >5k 3.5279347931005655E-4 1.076255116819478 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5980 0.5826744954214256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5003 0.48747834458083494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3525 0.34346615323754603 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1174 0.11439128053925647 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1153 0.11234509920082004 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.10883735976350041 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.359046362571641E-4 0.0 24-25 0.0 0.0 0.0 0.0010230906692182222 0.0 26-27 0.0 0.0 0.0 0.001364120892290963 0.0 28-29 0.0 0.0 0.0 0.002776960387878032 0.0 30-31 0.0 0.0 0.0 0.006138544015309334 0.0 32-33 0.0 0.0 0.0 0.012471962443803091 0.0 34-35 0.0 0.0 0.0 0.01900025528548127 0.0 36-37 0.0 0.0 0.0 0.025723422540343877 0.0 38-39 0.0 0.0 0.0 0.03152093633258047 0.0 40-41 0.0 0.0 0.0 0.03605176643911831 0.0 42-43 0.0 0.0 0.0 0.04141081280168995 0.0 44-45 0.0 0.0 0.0 0.04823141726314477 0.0 46-47 0.0 0.0 0.0 0.05714692166633213 0.0 48-49 0.0 0.0 0.0 0.06762142137499488 0.0 50-51 0.0 0.0 0.0 0.08160366052097726 0.0 52-53 0.0 0.0 0.0 0.10503730870640415 0.0 54-55 0.0 0.0 0.0 0.14693530754105516 0.0 56-57 0.0 0.0 0.0 0.22430044957527123 0.0 58-59 0.0 0.0 0.0 0.3125785587478149 0.0 60-61 0.0 0.0 0.0 0.39233091234354023 0.0 62-63 0.0 0.0 0.0 0.49273995373681434 0.0 64-65 0.0 0.0 0.0 0.6142928689605984 0.0 66-67 0.0 0.0 0.0 0.800592807964907 0.0 68-69 0.0 0.0 0.0 1.0098392091216817 0.0 70-71 0.0 0.0 0.0 1.1896595738876081 0.0 72-73 0.0 0.0 0.0 1.3714774013886752 0.0 74-75 0.0 0.0 0.0 1.5849136024289145 0.0 76-77 0.0 0.0 0.0 1.847506874194925 0.0 78-79 0.0 0.0 0.0 2.075315063207516 0.0 80-81 0.0 0.0 0.0 2.3089694846156394 0.0 82-83 0.0 0.0 0.0 2.556362552153265 0.0 84-85 0.0 0.0 0.0 2.88194897798114 0.0 86-87 0.0 0.0 0.0 3.2678490346895943 0.0 88-89 0.0 0.0 0.0 3.7255115940532124 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3890 0.0 35.554207 1 CATTCCG 315 0.0 34.68761 9 GTATCAA 5870 0.0 29.876925 1 TCAACGC 6900 0.0 25.264492 4 CAACGCA 6980 0.0 24.974928 5 ATCAACG 6990 0.0 24.871246 3 AACGCAG 7030 0.0 24.79609 6 TATCAAC 7170 0.0 24.313112 2 GTACATG 3785 0.0 22.727951 1 ACCGTGC 210 3.439891E-8 22.622355 7 TTAGGCA 580 0.0 22.112068 4 CCGTGCA 215 4.413596E-8 22.096252 8 GAGTACT 4775 0.0 22.087 12-13 ACGCAGA 7835 0.0 22.010235 7 CGCAGAG 7855 0.0 21.833235 8 TACATGG 3860 0.0 21.658033 2 TAGGCAT 600 0.0 21.375 5 AGTACTT 4885 0.0 20.957516 12-13 ACATGGG 3970 0.0 20.81864 3 GTGTAGC 605 0.0 20.425165 1 >>END_MODULE