FastQCFastQC Report
Thu 26 May 2016
SRR937648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007823
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.5147729313579864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38850.38548435588392005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.2794141431580744No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14260.14149309948274646No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12200.12105300236251802No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10230.10150591919414421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31550.033.923811
GTATCAA50550.033.688631
CATTCCG2750.031.0925359
TCAACGC58700.028.7523884
CAACGCA59200.028.5067065
AACGCAG60700.027.9380366
ATCAACG60600.027.8481373
TATCAAC61350.027.4302082
ACGCAGA68450.024.5666627
GTACATG38550.024.5555781
CGCAGAG68250.024.499458
TAAAACG1201.7002226E-423.7713414
GAGTACT40800.023.1656512-13
TACATGG39100.022.97872
AGTACTT42500.022.68603912-13
GCAGAGT72400.022.6358539
ACATGGG39550.021.8758683
GTACTTT44900.021.20791614-15
AGAGTAC65200.019.92429210-11
CATGGGG14550.019.6052274
Produced by FastQC (version 0.11.5)