##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937648_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1007823 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.767163480095213 33.0 31.0 34.0 30.0 34.0 2 31.88118747041891 33.0 31.0 34.0 30.0 34.0 3 31.923188893287808 33.0 31.0 34.0 30.0 34.0 4 35.54493993488936 37.0 35.0 37.0 33.0 37.0 5 35.56661636021405 37.0 35.0 37.0 33.0 37.0 6 35.6336886536624 37.0 35.0 37.0 33.0 37.0 7 35.57334075527151 37.0 35.0 37.0 33.0 37.0 8 35.57062599285787 37.0 35.0 37.0 33.0 37.0 9 37.148871379200514 39.0 37.0 39.0 34.0 39.0 10-11 37.084662187705575 39.0 37.0 39.0 33.0 39.0 12-13 37.01614321165522 39.0 37.0 39.0 33.0 39.0 14-15 38.153992814214405 40.0 38.0 41.0 33.0 41.0 16-17 38.069749350828474 40.0 38.0 41.0 33.0 41.0 18-19 38.04029427786427 40.0 38.0 41.0 33.0 41.0 20-21 38.0688171434865 40.0 38.0 41.0 33.5 41.0 22-23 38.06986445040448 40.0 38.0 41.0 33.0 41.0 24-25 38.024565821577795 40.0 38.0 41.0 33.0 41.0 26-27 37.939848068559655 40.0 38.0 41.0 33.0 41.0 28-29 37.82547481055701 40.0 38.0 41.0 33.0 41.0 30-31 37.6006377111854 40.0 37.0 41.0 33.0 41.0 32-33 37.456247277547746 40.0 37.0 41.0 32.5 41.0 34-35 37.409060916450606 40.0 37.0 41.0 32.5 41.0 36-37 37.25551014414238 40.0 37.0 41.0 32.0 41.0 38-39 37.1095762847246 40.0 36.0 41.0 31.0 41.0 40-41 36.91233530094074 39.5 36.0 41.0 31.0 41.0 42-43 36.669483133447045 39.0 36.0 41.0 30.5 41.0 44-45 36.4155779338237 39.0 36.0 41.0 30.0 41.0 46-47 36.06355034564601 39.0 35.5 41.0 29.5 41.0 48-49 35.73389672591318 39.0 35.0 40.0 28.5 41.0 50-51 34.88899241235812 38.0 34.0 39.5 26.5 40.5 52-53 35.019110994688546 38.0 35.0 40.0 27.0 40.5 54-55 35.348559221212454 39.0 35.0 40.0 27.0 41.0 56-57 35.167897041444775 39.0 35.0 40.0 26.5 41.0 58-59 34.951152632952414 38.5 35.0 40.0 26.0 41.0 60-61 34.66026623722618 38.0 34.0 40.0 25.0 41.0 62-63 34.32025812072159 38.0 34.0 40.0 23.5 41.0 64-65 33.95170530936484 38.0 34.0 40.0 22.5 41.0 66-67 33.49895715815178 37.0 33.0 39.5 22.0 41.0 68-69 33.03189349717162 36.5 32.5 39.0 20.5 40.5 70-71 32.603659075055845 36.0 32.0 39.0 18.5 40.0 72-73 32.12920721198068 35.5 31.0 38.5 17.5 40.0 74-75 31.57063194628422 35.0 31.0 38.0 12.0 39.5 76-77 31.009773045465323 35.0 30.5 37.0 8.0 39.0 78-79 30.470580647593874 34.5 30.0 36.5 6.5 39.0 80-81 29.889659692227703 34.0 29.0 36.0 2.0 38.5 82-83 29.314308663326795 34.0 29.0 35.5 2.0 37.0 84-85 28.737387914346073 34.0 28.5 35.0 2.0 37.0 86-87 28.152489574062113 33.5 27.5 35.0 2.0 36.0 88-89 27.57900742491489 33.0 26.0 35.0 2.0 36.0 90-91 26.9988743063018 33.0 25.5 35.0 2.0 35.5 92-93 26.35353926235063 32.0 25.0 34.5 2.0 35.0 94-95 25.56606417992048 32.0 22.0 34.0 2.0 35.0 96-97 24.654863006698598 31.5 17.0 34.0 2.0 35.0 98-99 23.464605392018242 31.0 2.0 34.0 2.0 35.0 100-101 20.114875826409996 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.269973848464268 1101 2 0.23306079206441055 1101 3 0.1134663572044694 1101 4 0.07474392614148684 1101 5 -0.05340301888797683 1101 6 0.13725201435416778 1101 7 0.2137569486417874 1101 8 0.32999308226121116 1101 9 0.4159861391385604 1101 10-11 0.277633286282871 1101 12-13 0.2630516276003405 1101 14-15 0.5582517399106379 1101 16-17 0.501605757206292 1101 18-19 0.6329446966945227 1101 20-21 0.5231706410182468 1101 22-23 0.48511837843509653 1101 24-25 0.5470129719027383 1101 26-27 0.44595854242579236 1101 28-29 0.4782306117907851 1101 30-31 0.5380907267827766 1101 32-33 0.621304713539125 1101 34-35 0.8733275899742168 1101 36-37 0.7295546977759599 1101 38-39 0.6600827039165296 1101 40-41 0.6186377955171238 1101 42-43 0.6513359665864229 1101 44-45 0.7773917176682446 1101 46-47 0.6764297165030868 1101 48-49 0.6855877356010609 1101 50-51 0.4911560240895838 1101 52-53 0.7424006246102834 1101 54-55 0.8694848097372017 1101 56-57 0.8955531155492693 1101 58-59 0.9246145581389769 1101 60-61 0.9746449555418764 1101 62-63 0.98244241272198 1101 64-65 0.9706624844465281 1101 66-67 1.0544447057606519 1101 68-69 1.3295640923002594 1101 70-71 1.0725856884328024 1101 72-73 1.131244974850489 1101 74-75 1.0886578172203194 1101 76-77 1.0986725235393777 1101 78-79 1.3614004714632024 1101 80-81 1.5364149761101942 1101 82-83 1.7033531584022263 1101 84-85 1.7989006153787592 1101 86-87 1.9296938145021407 1101 88-89 2.1598706320293424 1101 90-91 2.234543575518458 1101 92-93 2.06615276081439 1101 94-95 2.301159020683933 1101 96-97 2.448513681981833 1101 98-99 2.833327584663852 1101 100-101 3.40120098359505 1102 1 0.3261115092530886 1102 2 0.2698537934681262 1102 3 0.25493459574045474 1102 4 0.12928897664205152 1102 5 0.0827096045856166 1102 6 0.32694301092079314 1102 7 0.42109999319413305 1102 8 0.38926411119857107 1102 9 0.572717510227875 1102 10-11 0.6478688814931886 1102 12-13 0.5512066811750103 1102 14-15 0.6796308663453843 1102 16-17 0.635605394239299 1102 18-19 0.6731766251974349 1102 20-21 0.712326446453126 1102 22-23 0.6717574892400293 1102 24-25 0.5840911616604032 1102 26-27 0.6969724425802397 1102 28-29 0.7669319677317716 1102 30-31 0.7127537785944682 1102 32-33 0.8449090498836043 1102 34-35 0.8879684933916607 1102 36-37 0.8216001649478173 1102 38-39 0.8426051023135486 1102 40-41 1.0591315787813826 1102 42-43 1.0668220645801014 1102 44-45 1.1881481334802615 1102 46-47 1.0564802355829244 1102 48-49 0.9952652505364057 1102 50-51 0.974928066001155 1102 52-53 1.0199740791301508 1102 54-55 1.18269199352072 1102 56-57 1.2678183629409077 1102 58-59 1.324089367118333 1102 60-61 1.4162406029009205 1102 62-63 1.3194461572080343 1102 64-65 1.5095166198628434 1102 66-67 1.555315085801908 1102 68-69 1.6545695553239312 1102 70-71 1.5896877303073396 1102 72-73 1.5574811922899414 1102 74-75 1.6994627520899677 1102 76-77 1.652258304122121 1102 78-79 1.5480636566097026 1102 80-81 1.6493217861858582 1102 82-83 2.2225996685486322 1102 84-85 2.2704391880295667 1102 86-87 2.549943298755668 1102 88-89 2.3133908563281302 1102 90-91 2.2248045784184924 1102 92-93 2.1963875412008385 1102 94-95 2.479390630664785 1102 96-97 2.8688109445440126 1102 98-99 3.397957932408456 1102 100-101 4.003437304739357 1103 1 0.2100803870638117 1103 2 0.14141575592552513 1103 3 0.09586072762710884 1103 4 0.18241055320719113 1103 5 0.045109784824433063 1103 6 0.20063756096150343 1103 7 0.23589268875384306 1103 8 0.14891946406493162 1103 9 0.2496620808315413 1103 10-11 0.35017145621421264 1103 12-13 0.30494764986327993 1103 14-15 0.43668217361322803 1103 16-17 0.5240409046238881 1103 18-19 0.6213806145309135 1103 20-21 0.6822747231578532 1103 22-23 0.5712546801608553 1103 24-25 0.6129281802338227 1103 26-27 0.47615187914320956 1103 28-29 0.522779298778751 1103 30-31 0.4674168023390237 1103 32-33 0.5608940395186792 1103 34-35 0.5918535220069572 1103 36-37 0.5579985118050246 1103 38-39 0.507798388179225 1103 40-41 0.5171861200962411 1103 42-43 0.5580252898059115 1103 44-45 0.6409039873676221 1103 46-47 0.4466019087045723 1103 48-49 0.46966406706354746 1103 50-51 0.483634840572698 1103 52-53 0.44131747818151723 1103 54-55 0.5495054022396602 1103 56-57 0.3268370388769952 1103 58-59 0.4949812750868645 1103 60-61 0.9273446014132318 1103 62-63 0.8161453550737718 1103 64-65 0.6446361294034588 1103 66-67 0.8196313763154066 1103 68-69 0.9067721761775118 1103 70-71 0.79310606913009 1103 72-73 0.8577469014974994 1103 74-75 1.0224252011444968 1103 76-77 1.0989263297322438 1103 78-79 1.0800620068046634 1103 80-81 1.316744216143647 1103 82-83 1.4179471944967048 1103 84-85 1.628211450563139 1103 86-87 1.6738145710485988 1103 88-89 1.8952707757928522 1103 90-91 1.9563917886148587 1103 92-93 2.099210567424869 1103 94-95 2.0993614968243506 1103 96-97 2.2617375246435962 1103 98-99 2.6829075144141363 1103 100-101 3.4916603908045865 1104 1 0.1928653509012257 1104 2 0.2597325721946895 1104 3 0.17750788221437475 1104 4 0.1766803625640918 1104 5 0.10096830149124969 1104 6 0.1840252345463611 1104 7 0.17198432732371316 1104 8 0.19587522383505274 1104 9 0.18553131728057792 1104 10-11 0.22725703210226555 1104 12-13 0.12694366441728278 1104 14-15 0.2726077889998919 1104 16-17 0.3648321224089415 1104 18-19 0.15789125869641651 1104 20-21 0.21718685207991228 1104 22-23 0.11269987138220472 1104 24-25 0.08716345054136099 1104 26-27 0.24748302255699883 1104 28-29 0.2449334412406543 1104 30-31 0.2754654238906724 1104 32-33 0.27117468875495376 1104 34-35 0.49413892620057 1104 36-37 0.352369958931142 1104 38-39 0.5098689519241191 1104 40-41 0.6741436368431195 1104 42-43 0.8176686457649467 1104 44-45 0.8142075039899552 1104 46-47 0.6025107994517569 1104 48-49 0.7879215019664443 1104 50-51 0.5949915824705911 1104 52-53 0.5342509843954275 1104 54-55 0.5550309829315907 1104 56-57 0.7136560435830219 1104 58-59 0.740304714491181 1104 60-61 0.7047623348687395 1104 62-63 0.9752662884509959 1104 64-65 0.8357718480736764 1104 66-67 0.9981090782787803 1104 68-69 0.9474317319070309 1104 70-71 0.8494388583195658 1104 72-73 0.872943082408284 1104 74-75 0.6835332974712962 1104 76-77 1.0870583965596339 1104 78-79 1.1279357313285026 1104 80-81 1.4143926473190902 1104 82-83 1.5009515580263155 1104 84-85 1.5598772869285575 1104 86-87 1.6110910569171715 1104 88-89 1.5532318821099622 1104 90-91 1.8116964127997548 1104 92-93 1.891693820545175 1104 94-95 2.1179570941519685 1104 96-97 2.28469109152881 1104 98-99 2.4739345896483265 1104 100-101 2.955294864022921 1105 1 0.11827771850726876 1105 2 0.22485884907985465 1105 3 0.10526441421992061 1105 4 0.03429162481962322 1105 5 0.05370002299767407 1105 6 0.07498095208310218 1105 7 0.17457873555053283 1105 8 0.2699516743937167 1105 9 0.32910924791384844 1105 10-11 0.26724150415196135 1105 12-13 0.13763356739967492 1105 14-15 0.30200074452920944 1105 16-17 0.4366670727023916 1105 18-19 0.31310873555421637 1105 20-21 0.26855207118229174 1105 22-23 0.24097122124446457 1105 24-25 0.1464316691475247 1105 26-27 0.2292440974504828 1105 28-29 0.41277432894320043 1105 30-31 0.3424783681269403 1105 32-33 0.3576082650600725 1105 34-35 0.45928873028318407 1105 36-37 0.4281532007222921 1105 38-39 0.43560428378593485 1105 40-41 0.4253745370364683 1105 42-43 0.5189502363746072 1105 44-45 0.4306008273990898 1105 46-47 0.3704096496134568 1105 48-49 0.47166035863020284 1105 50-51 0.401332803831572 1105 52-53 0.463375302106698 1105 54-55 0.5377042714349187 1105 56-57 0.400251315899645 1105 58-59 0.6273538478347902 1105 60-61 0.7194689721050267 1105 62-63 0.7382554402741448 1105 64-65 0.7384997196750689 1105 66-67 0.7749814154277956 1105 68-69 0.6574825506534978 1105 70-71 0.5744968207787124 1105 72-73 0.711472718121712 1105 74-75 0.8067259809406657 1105 76-77 0.85173829834428 1105 78-79 1.0271912024713252 1105 80-81 1.1934861657900768 1105 82-83 1.0445699126624604 1105 84-85 1.348789132658581 1105 86-87 1.378172871676849 1105 88-89 1.3764656312330388 1105 90-91 1.4251392117662647 1105 92-93 1.8062387617699578 1105 94-95 2.047376571249309 1105 96-97 1.8135001447943857 1105 98-99 1.890422663498061 1105 100-101 2.4766907112586907 1106 1 0.12557010406052527 1106 2 0.09210062501728444 1106 3 0.021367473801237935 1106 4 -0.01093496823305884 1106 5 0.10773729007841837 1106 6 0.04553535742012116 1106 7 0.07073700562184371 1106 8 0.11007129712204033 1106 9 0.18024796426995238 1106 10-11 -0.1799519626067294 1106 12-13 -0.28178212158123017 1106 14-15 -0.4921791496506813 1106 16-17 -0.12853881641653686 1106 18-19 0.14367004220247992 1106 20-21 0.1125479303955359 1106 22-23 -0.18812930785606596 1106 24-25 -0.30234909919845876 1106 26-27 -0.3240685319491021 1106 28-29 -0.06401426088887519 1106 30-31 -0.09654854698693072 1106 32-33 -0.14502573540001862 1106 34-35 -0.16766089014470253 1106 36-37 -0.43400460926160633 1106 38-39 -0.33775665479239336 1106 40-41 -0.17646581262996364 1106 42-43 0.004215936857690394 1106 44-45 -0.03921743807135414 1106 46-47 0.06688998087638964 1106 48-49 0.17670981802748997 1106 50-51 -0.02654849361493916 1106 52-53 0.2187928314372769 1106 54-55 0.32938819137971365 1106 56-57 0.13866861518651064 1106 58-59 0.0016579656171629154 1106 60-61 0.2472237455119668 1106 62-63 -0.11172155535503236 1106 64-65 0.34281303920577244 1106 66-67 0.5330559495892828 1106 68-69 0.4341561186314209 1106 70-71 0.46258055668853615 1106 72-73 0.3086316783241472 1106 74-75 0.3547476050492442 1106 76-77 0.996665834576163 1106 78-79 0.88357491537678 1106 80-81 0.651905765514023 1106 82-83 0.589106374590223 1106 84-85 0.9474663813358326 1106 86-87 1.087029554446577 1106 88-89 1.2350347593673838 1106 90-91 1.3764964696452644 1106 92-93 1.5283919708796851 1106 94-95 1.3948575926433762 1106 96-97 1.61753741187513 1106 98-99 1.587708147740063 1106 100-101 1.5218942999405414 1107 1 0.05630741897050129 1107 2 0.2969576917448542 1107 3 0.22732080637327456 1107 4 0.3373882779319146 1107 5 0.21880272344601082 1107 6 0.21263976912884885 1107 7 0.23491804174532405 1107 8 0.31211918262315663 1107 9 0.37387591904898443 1107 10-11 0.4784774843119237 1107 12-13 0.3707092186798917 1107 14-15 0.5030970103736792 1107 16-17 0.4319118706528542 1107 18-19 0.4337389394656199 1107 20-21 0.45660411402006673 1107 22-23 0.4184292548176032 1107 24-25 0.4611277383790977 1107 26-27 0.2927522971019414 1107 28-29 0.3792235298309308 1107 30-31 0.07727810597641138 1107 32-33 0.15119823780237596 1107 34-35 0.182127850291522 1107 36-37 0.23827280690070296 1107 38-39 0.38484240881386 1107 40-41 0.39308235835705574 1107 42-43 0.223274975341198 1107 44-45 0.23292294486039822 1107 46-47 0.08789032345946879 1107 48-49 0.32545558332002145 1107 50-51 0.3850079754954905 1107 52-53 0.12460532430013416 1107 54-55 0.17845750735550325 1107 56-57 0.2600305732391135 1107 58-59 0.06664216873086559 1107 60-61 0.23943188376327385 1107 62-63 0.5298718365980761 1107 64-65 0.37190976182767344 1107 66-67 0.4642995261566014 1107 68-69 0.4286519505373292 1107 70-71 0.5341613872621807 1107 72-73 0.7228412630373313 1107 74-75 0.7135843451809443 1107 76-77 0.566038907546357 1107 78-79 0.5884167561898401 1107 80-81 0.7735798548140167 1107 82-83 0.9263489614730993 1107 84-85 1.2233180809285393 1107 86-87 1.2732158435105632 1107 88-89 1.3141676435362406 1107 90-91 1.4792561666495665 1107 92-93 1.7944419880088383 1107 94-95 1.8545469839874436 1107 96-97 2.1330221669282317 1107 98-99 2.2924662460233414 1107 100-101 3.130093454902287 1108 1 0.008322903862175934 1108 2 -0.04082546443921231 1108 3 0.02723452872113441 1108 4 -0.038093703666966405 1108 5 0.11649197839442138 1108 6 0.1061524479474798 1108 7 0.22546541299232814 1108 8 0.020140032714202505 1108 9 0.3329042810339473 1108 10-11 0.2337999034881122 1108 12-13 0.22742939730126466 1108 14-15 0.32032671898678444 1108 16-17 0.31286817952684487 1108 18-19 0.30873727436841136 1108 20-21 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-1.9558315659950445 2316 76-77 -1.9013663657816195 2316 78-79 -1.9329398360216956 2316 80-81 -2.1959163090522367 2316 82-83 -2.4347817097629765 2316 84-85 -2.6661184403885265 2316 86-87 -3.0231464022410073 2316 88-89 -3.161244972958862 2316 90-91 -2.965075309135816 2316 92-93 -3.264192576356024 2316 94-95 -3.4736718107697477 2316 96-97 -3.4677381652609114 2316 98-99 -3.9813972085745952 2316 100-101 -3.8784521236616953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3474.0 3 461.0 4 435.0 5 475.0 6 615.0 7 659.0 8 916.0 9 1100.0 10 1503.0 11 2100.0 12 3085.0 13 4272.0 14 4925.0 15 6339.0 16 8529.0 17 11349.0 18 9394.0 19 6232.0 20 6119.0 21 6492.0 22 7172.0 23 8171.0 24 9262.0 25 10919.0 26 12352.0 27 14820.0 28 17540.0 29 21592.0 30 26115.0 31 32477.0 32 41014.0 33 52654.0 34 70666.0 35 98100.0 36 140014.0 37 201165.0 38 152255.0 39 13059.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.185621594390334 22.203405186532578 15.32762052112237 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32.2023922847995 18.648100105531743 20-21 19.79520894920687 29.302846485252093 32.189446883222175 18.712497682318865 22-23 19.44738164729604 29.564500228927827 31.553661826123225 19.434456297652904 24-25 19.168880079976176 29.71814959144442 32.11559560329867 18.997374725280736 26-27 19.177175731988758 29.73156517874277 32.162910315903346 18.928348773365126 28-29 18.723446992428304 29.78854048636036 32.37332739358039 19.11468512763094 30-31 19.400383250348792 29.489393989115054 32.174234717469716 18.93598804306644 32-33 19.03280257548073 29.614547985730443 32.04817839430213 19.304471044486707 34-35 19.4767814756956 29.743168784884467 31.86524813526895 18.914801604150984 36-37 19.520904442374214 29.284115686062012 31.63738788674784 19.557591984815932 38-39 18.88961461147994 30.151487134827327 31.632209755699904 19.32668849799283 40-41 19.344057775800668 29.745888867074406 31.896228220377104 19.013825136747826 42-43 19.17595552681172 29.75146137055406 31.5781310662599 19.49445203637432 44-45 19.48101337559543 29.905138960833654 31.263130265155546 19.35071739841537 46-47 19.528119862208374 29.987178359018873 30.774573278984292 19.71012849978846 48-49 19.396589014083666 29.913795589444657 30.959107348306986 19.730508048164687 50-51 19.40629393854421 30.054013956920087 30.61279788717129 19.926894217364413 52-53 19.526505997736553 30.467974612797427 30.54410240804498 19.46141698142104 54-55 19.802853439674017 29.98980310699479 30.383857587537417 19.823485865793774 56-57 19.85909682806334 30.45163566962555 30.035884335145774 19.653383167165337 58-59 19.744431966129657 30.13511364652799 30.301135968355876 19.819318418986473 60-61 19.94930467887993 30.122265186230752 30.249550203278297 19.678879931611018 62-63 19.79998290367213 29.953909492835447 30.554418009192258 19.69168959430016 64-65 19.69230601009245 29.944000966203266 30.312142302257605 20.05155072144668 66-67 19.75250779936412 30.027489745395552 30.13613661474049 20.083865840499833 68-69 19.682291335326674 29.776862395826708 30.408810595457723 20.13203567338889 70-71 19.6729100863078 29.948682519998908 30.641381521234006 19.737025872459288 72-73 19.91152311955563 29.491581027176817 30.613199458203372 19.983696395064182 74-75 19.590127141413443 30.073117373043758 30.21389404070295 20.122861444839852 76-77 19.551855957606517 29.93607174281553 30.425277014162646 20.08679528541531 78-79 19.339541278794346 29.83228804363225 30.34567880369216 20.482491873881244 80-81 19.413468774635973 29.79529713280783 30.26035468840249 20.53087940415371 82-83 19.49596196006841 30.138653701731272 30.257839125522928 20.107545212677394 84-85 19.61813873321896 29.67478624237858 30.16482347725651 20.542251547145955 86-87 19.369611386100477 29.789706445884363 30.31406110446487 20.526621063550294 88-89 19.96729305275611 29.469953598882597 30.144869905383477 20.417883442977825 90-91 19.611115888340088 29.21392602563102 30.650557519189963 20.524400566838928 92-93 19.724400838974542 29.378025626497283 30.689917394805118 20.20765613972306 94-95 19.724782498051628 29.25575167400951 30.569362047301702 20.450103780637157 96-97 19.92681151928231 28.992410746240164 30.260732719883563 20.820045014593955 98-99 19.6165672684904 29.06792711308527 30.200907258966463 21.114598359457872 100-101 19.892556479907764 28.409816024092795 31.174772142211086 20.52285535378835 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1794.0 1 1051.5 2 310.5 3 321.5 4 305.5 5 315.5 6 475.5 7 880.5 8 1333.0 9 1451.0 10 1204.5 11 996.0 12 1014.0 13 1033.5 14 1022.5 15 1088.5 16 1170.5 17 1253.5 18 1429.0 19 1684.5 20 1955.0 21 2399.5 22 3021.5 23 3646.5 24 4464.0 25 6116.5 26 9316.5 27 12820.5 28 14384.5 29 16632.0 30 20858.5 31 24740.0 32 29527.0 33 35260.0 34 41169.5 35 45427.0 36 48167.5 37 50447.0 38 48347.0 39 44943.5 40 43514.5 41 42248.0 42 40737.0 43 39886.5 44 39046.0 45 37123.5 46 34922.5 47 32328.0 48 31357.5 49 29420.5 50 25485.0 51 21971.0 52 19029.5 53 16720.0 54 14447.0 55 12365.5 56 10379.5 57 8359.5 58 6538.5 59 5208.0 60 4056.5 61 3060.0 62 2269.5 63 1500.0 64 879.5 65 594.5 66 397.5 67 258.5 68 198.5 69 148.5 70 116.5 71 82.5 72 64.0 73 56.5 74 46.0 75 29.0 76 22.0 77 16.5 78 13.5 79 10.5 80 6.0 81 8.0 82 5.5 83 3.0 84 1.5 85 0.0 86 0.0 87 1.5 88 1.5 89 0.0 90 0.0 91 0.5 92 2.0 93 2.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3387499590701939 2 0.2922140098013242 3 0.29697675087788233 4 0.2923132335737525 5 0.2923132335737525 6 0.2141249009002573 7 0.2045994187471411 8 0.2122396492241197 9 0.2139264533554007 10-11 0.211991589793049 12-13 0.21586131691775243 14-15 0.2102055618893397 16-17 0.20365679290907232 18-19 0.19492510093538248 20-21 0.19532199602509567 22-23 0.20316067404693086 24-25 0.20077930350865184 26-27 0.20876681718912946 28-29 0.21526597428318267 30-31 0.21968143215624175 32-33 0.21685355464203535 34-35 0.21650627143853635 36-37 0.2016227055742923 38-39 0.19140265701417808 40-41 0.18510194746498143 42-43 0.19879482806008597 44-45 0.20201960066400548 46-47 0.20851875775805873 48-49 0.2173000616179627 50-51 0.214025677127829 52-53 0.2259325298192242 54-55 0.21080090452390948 56-57 0.17989269941249605 58-59 0.16203242037540322 60-61 0.17989269941249605 62-63 0.17453461570136822 64-65 0.18093454902299314 66-67 0.1797438637538536 68-69 0.1792973567779263 70-71 0.1816291154299912 72-73 0.18956701722425465 74-75 0.19591733865966543 76-77 0.19924133503601327 78-79 0.19462742961809762 80-81 0.1911049856968932 82-83 0.1813314441127063 84-85 0.17632064360507746 86-87 0.18103377279542143 88-89 0.1905592549485376 90-91 0.18788021309297367 92-93 0.18187717486106192 94-95 0.18406009785448435 96-97 0.19120420946932148 98-99 0.19209722342117616 100-101 0.1699703221696667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1007823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.77693104188185 #Duplication Level Percentage of deduplicated Percentage of total 1 79.46420695135764 45.117337983750616 2 12.640806471283453 14.354123946676689 3 3.768958914437839 6.419697611541691 4 1.5243790603905047 3.4619825917392135 5 0.7292709200465721 2.070288236916698 6 0.39582921580847785 1.348438085419207 7 0.2434505725902142 0.967566346044287 8 0.1562096987402583 0.7095285834759029 9 0.10756016102880467 0.5496242261027535 >10 0.7682142054170052 8.909884947131589 >50 0.11660802964938409 4.706478789841518 >100 0.07959674890426612 8.355552500846883 >500 0.003857110985836275 1.4808038619646213 >1k 8.766161331446081E-4 1.0322624394039475 >5k 1.753232266289216E-4 0.5164298491444242 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5188 0.5147729313579864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3885 0.38548435588392005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2816 0.2794141431580744 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1426 0.14149309948274646 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1220 0.12105300236251802 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1023 0.10150591919414421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.922377242829346E-5 0.0 5 0.0 0.0 0.0 9.922377242829346E-5 0.0 6 0.0 0.0 0.0 9.922377242829346E-5 0.0 7 0.0 0.0 0.0 9.922377242829346E-5 0.0 8 0.0 0.0 0.0 9.922377242829346E-5 0.0 9 0.0 0.0 0.0 9.922377242829346E-5 0.0 10-11 0.0 0.0 0.0 1.984475448565869E-4 0.0 12-13 0.0 0.0 0.0 1.984475448565869E-4 0.0 14-15 0.0 0.0 0.0 1.984475448565869E-4 0.0 16-17 0.0 0.0 0.0 1.984475448565869E-4 0.0 18-19 0.0 0.0 0.0 2.4805943107073365E-4 0.0 20-21 0.0 0.0 0.0 4.4650697592732056E-4 0.0 22-23 0.0 0.0 0.0 4.961188621414673E-4 0.0 24-25 0.0 0.0 0.0 7.937901794263476E-4 0.0 26-27 0.0 0.0 0.0 0.001289909041567815 0.0 28-29 0.0 0.0 0.0 0.0027782656279922166 0.0 30-31 0.0 0.0 0.0 0.006151873890554195 0.0 32-33 0.0 0.0 0.0 0.012402971553536682 0.0 34-35 0.0 0.0 0.0 0.0190013524200182 0.0 36-37 0.0 0.0 0.0 0.025004390651929952 0.0 38-39 0.0 0.0 0.0 0.03021363870441536 0.0 40-41 0.0 0.0 0.0 0.034331425260189535 0.0 42-43 0.0 0.0 0.0 0.03934222576781836 0.0 44-45 0.0 0.0 0.0 0.04415457873059059 0.0 46-47 0.0 0.0 0.0 0.05293588259049456 0.0 48-49 0.0 0.0 0.0 0.06280864794710976 0.0 50-51 0.0 0.0 0.0 0.07064732596894494 0.0 52-53 0.0 0.0 0.0 0.08880527632332265 0.0 54-55 0.0 0.0 0.0 0.12447622251129414 0.0 56-57 0.0 0.0 0.0 0.19041041928989516 0.0 58-59 0.0 0.0 0.0 0.2639848465454747 0.0 60-61 0.0 0.0 0.0 0.3341856655384924 0.0 62-63 0.0 0.0 0.0 0.4189723790784691 0.0 64-65 0.0 0.0 0.0 0.5340719550952895 0.0 66-67 0.0 0.0 0.0 0.708358511365587 0.0 68-69 0.0 0.0 0.0 0.9068060562221739 0.0 70-71 0.0 0.0 0.0 1.0849623396171748 0.0 72-73 0.0 0.0 0.0 1.2669387382506652 0.0 74-75 0.0 0.0 0.0 1.4716373807702343 0.0 76-77 0.0 0.0 0.0 1.724409941031312 0.0 78-79 0.0 0.0 0.0 1.9467704150431175 0.0 80-81 0.0 0.0 0.0 2.1732982874969116 0.0 82-83 0.0 0.0 0.0 2.40885552324168 0.0 84-85 0.0 0.0 0.0 2.7327715283338447 0.0 86-87 0.0 0.0 0.0 3.129914677478089 0.0 88-89 0.0 0.0 0.0 3.5832680937029617 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3155 0.0 33.92381 1 GTATCAA 5055 0.0 33.68863 1 CATTCCG 275 0.0 31.092535 9 TCAACGC 5870 0.0 28.752388 4 CAACGCA 5920 0.0 28.506706 5 AACGCAG 6070 0.0 27.938036 6 ATCAACG 6060 0.0 27.848137 3 TATCAAC 6135 0.0 27.430208 2 ACGCAGA 6845 0.0 24.566662 7 GTACATG 3855 0.0 24.555578 1 CGCAGAG 6825 0.0 24.49945 8 TAAAACG 120 1.7002226E-4 23.771341 4 GAGTACT 4080 0.0 23.16565 12-13 TACATGG 3910 0.0 22.9787 2 AGTACTT 4250 0.0 22.686039 12-13 GCAGAGT 7240 0.0 22.635853 9 ACATGGG 3955 0.0 21.875868 3 GTACTTT 4490 0.0 21.207916 14-15 AGAGTAC 6520 0.0 19.924292 10-11 CATGGGG 1455 0.0 19.605227 4 >>END_MODULE