FastQCFastQC Report
Thu 26 May 2016
SRR937648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007823
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58560.5810544113400865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47860.47488497484181247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33760.3349794557179187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11000.10914614967112281No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.10557409386370423No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10540.10458185613942131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10410.1032919470978535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.041.5096361
CGTACAC608.2827566E-539.589233
CACACCG608.2827566E-539.589235
GTATCAA50600.032.7831
ACCGCCC856.406629E-427.9411758
TCAACGC62050.026.7968984
ATCAACG63000.026.3928183
CAACGCA63200.026.2341295
TATCAAC64500.025.8526882
AACGCAG64500.025.7739166
ACACCGT950.001226804524.998766
TAGGACG2101.2623786E-924.8846594
GAGTACT47300.023.49894112-13
GTACATG34850.023.458461
TACATGG35300.022.744182
ACGCAGA72850.022.6252577
ACACCGC1050.002196668222.6179266
CGCAGAG72900.022.5445828
ACATGGG34800.022.3883913
GTACTTT50500.022.00880814-15
Produced by FastQC (version 0.11.5)