##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1007823 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1149229577019 33.0 31.0 34.0 31.0 34.0 2 32.32714474664698 34.0 31.0 34.0 31.0 34.0 3 32.44965832293964 34.0 31.0 34.0 31.0 34.0 4 36.102458467409456 37.0 35.0 37.0 35.0 37.0 5 35.93586175350235 37.0 35.0 37.0 35.0 37.0 6 36.024391187738324 37.0 35.0 37.0 35.0 37.0 7 35.98919949237118 37.0 35.0 37.0 35.0 37.0 8 35.98811299206309 37.0 35.0 37.0 35.0 37.0 9 37.62495001602464 39.0 37.0 39.0 35.0 39.0 10-11 37.59083638694493 39.0 37.0 39.0 35.0 39.0 12-13 37.531100203111066 39.0 37.0 39.0 35.0 39.0 14-15 38.753931990041906 40.0 38.0 41.0 35.0 41.0 16-17 38.72602828075962 40.0 38.0 41.0 35.0 41.0 18-19 38.743030770284065 40.0 38.0 41.0 35.0 41.0 20-21 38.688023591444136 40.0 38.0 41.0 34.0 41.0 22-23 38.54782089712182 40.0 38.0 41.0 34.0 41.0 24-25 38.50878179997876 40.0 38.0 41.0 34.0 41.0 26-27 38.43199202637764 40.0 38.0 41.0 34.0 41.0 28-29 38.336030731586796 40.0 38.0 41.0 34.0 41.0 30-31 38.36295212552204 40.0 38.0 41.0 34.0 41.0 32-33 38.27284801001763 40.0 38.0 41.0 34.0 41.0 34-35 38.14813761940341 40.0 38.0 41.0 34.0 41.0 36-37 37.99279883471601 40.0 37.0 41.0 33.0 41.0 38-39 37.86389127852807 40.0 37.0 41.0 33.0 41.0 40-41 37.701628162881775 40.0 37.0 41.0 33.0 41.0 42-43 37.512277949600275 40.0 37.0 41.0 33.0 41.0 44-45 37.199026019449846 40.0 37.0 41.0 32.5 41.0 46-47 36.84515088462955 40.0 37.0 41.0 31.0 41.0 48-49 36.54109650206435 40.0 36.5 41.0 30.5 41.0 50-51 36.33037745715269 39.5 36.0 41.0 30.0 41.0 52-53 36.15760555176851 39.0 36.0 41.0 30.0 41.0 54-55 35.98672336313023 39.0 36.0 40.5 29.5 41.0 56-57 35.75101977232113 39.0 35.0 40.0 28.5 41.0 58-59 35.55353618641368 39.0 35.0 40.0 28.0 41.0 60-61 35.2738313176024 38.5 35.0 40.0 27.5 41.0 62-63 34.95468648760745 38.0 34.5 40.0 27.0 41.0 64-65 34.60383172441986 38.0 34.0 40.0 26.0 41.0 66-67 34.21539694966279 37.5 34.0 40.0 26.0 41.0 68-69 33.78899816733692 37.0 33.5 39.0 25.0 40.5 70-71 33.410093835921586 36.0 33.0 39.0 24.5 40.0 72-73 32.97580428309336 36.0 32.5 39.0 23.5 40.0 74-75 32.46293297533396 35.0 32.0 38.0 22.0 39.5 76-77 31.11955472339885 34.0 30.5 36.0 21.0 39.0 78-79 31.698340879301227 35.0 31.0 37.0 21.5 39.0 80-81 31.478722454240476 35.0 31.0 36.0 20.5 39.0 82-83 31.08869761853024 34.5 31.0 36.0 19.5 37.5 84-85 30.653312139135544 34.0 31.0 35.5 18.0 37.0 86-87 30.213295886281617 34.0 30.5 35.0 14.0 36.5 88-89 29.750383747939868 34.0 30.0 35.0 7.5 36.0 90-91 29.248506930284385 34.0 29.0 35.0 3.5 36.0 92-93 28.83188218566157 34.0 29.0 35.0 2.0 35.0 94-95 28.362024879368697 34.0 29.0 35.0 2.0 35.0 96-97 27.826441746219324 33.0 28.5 35.0 2.0 35.0 98-99 27.01257958986846 33.0 26.5 35.0 2.0 35.0 100-101 24.56600117282499 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2821076238344915 1101 2 0.18845622968380837 1101 3 0.1539804943505274 1101 4 0.12125077357456604 1101 5 0.039087518615836814 1101 6 0.08554600747008578 1101 7 -0.03516784681683305 1101 8 0.05933244237147761 1101 9 0.3028117884929884 1101 10-11 0.25168313570309664 1101 12-13 0.24760575318168065 1101 14-15 0.38722107748694157 1101 16-17 0.276317627788373 1101 18-19 0.3989736561512416 1101 20-21 0.2635185714396755 1101 22-23 0.22804771401543888 1101 24-25 0.21204402769449615 1101 26-27 0.32455772478912337 1101 28-29 0.4140993634438459 1101 30-31 0.26051900915017967 1101 32-33 0.23823869090087157 1101 34-35 0.04673471426707465 1101 36-37 0.1581322624774799 1101 38-39 0.3035967774312027 1101 40-41 0.2197200596992559 1101 42-43 0.309799569262708 1101 44-45 0.486308101285708 1101 46-47 0.26958603868839504 1101 48-49 0.28273387588044585 1101 50-51 0.4039817604086693 1101 52-53 0.4464046674257318 1101 54-55 0.25386108022476606 1101 56-57 0.1859059857535641 1101 58-59 0.3236568814432772 1101 60-61 0.48834140827630534 1101 62-63 0.3486195565690551 1101 64-65 0.5155762419669543 1101 66-67 0.5942714675788139 1101 68-69 0.4603810324845341 1101 70-71 0.5300234242360347 1101 72-73 0.5844092207647549 1101 74-75 0.7309711182988394 1101 76-77 0.41485414966337686 1101 78-79 0.5769837227143775 1101 80-81 0.6800381467177097 1101 82-83 0.7009463400605789 1101 84-85 0.7335021935465669 1101 86-87 0.852155655010872 1101 88-89 0.916201529086436 1101 90-91 0.8200284804433622 1101 92-93 1.133258858393404 1101 94-95 1.3178540133388168 1101 96-97 1.378727588643983 1101 98-99 1.6202659982148724 1101 100-101 2.210644357543867 1102 1 0.28379653148901696 1102 2 0.39752521933925067 1102 3 0.3093155536289629 1102 4 0.11305808995215472 1102 5 0.15552214566724842 1102 6 0.13023168916284789 1102 7 0.18213827139929606 1102 8 0.23067138317452418 1102 9 0.296596904623911 1102 10-11 0.2769982317967603 1102 12-13 0.29886558199459046 1102 14-15 0.27655318117698613 1102 16-17 0.38084656699881236 1102 18-19 0.498128461575007 1102 20-21 0.3273433546779998 1102 22-23 0.3584843484965674 1102 24-25 0.2491962923492892 1102 26-27 0.17551903176660488 1102 28-29 0.23403439975906792 1102 30-31 0.3099325211521631 1102 32-33 0.40137568871936935 1102 34-35 0.4981508411129312 1102 36-37 0.3462158337764194 1102 38-39 0.265618577673429 1102 40-41 0.38385151301169884 1102 42-43 0.3423610421679655 1102 44-45 0.3048894188559075 1102 46-47 0.4663752622723578 1102 48-49 0.5523609458108467 1102 50-51 0.5500926431221771 1102 52-53 0.47156976185270594 1102 54-55 0.5605404136988454 1102 56-57 0.7703254422660422 1102 58-59 0.7849360808417956 1102 60-61 0.6358430471834069 1102 62-63 0.6098298663132411 1102 64-65 0.7027783219900741 1102 66-67 0.8272685008791782 1102 68-69 0.8021866821769379 1102 70-71 0.7808391740406648 1102 72-73 0.7237274354115826 1102 74-75 0.7519657960174726 1102 76-77 0.5545094050921406 1102 78-79 0.866175824848856 1102 80-81 0.8524882109776861 1102 82-83 0.9096319921233835 1102 84-85 1.0142053124701107 1102 86-87 0.9195841819945088 1102 88-89 0.9392536370728202 1102 90-91 1.2042086750381173 1102 92-93 1.4311325384713314 1102 94-95 1.2223640634504847 1102 96-97 1.5498109710519365 1102 98-99 1.6716165687767663 1102 100-101 2.154778922001988 1103 1 0.28835137618204953 1103 2 0.26044358617530605 1103 3 0.18637408475412798 1103 4 0.11645425653954788 1103 5 0.16691223753898043 1103 6 0.17762305415002544 1103 7 0.20725726835597413 1103 8 0.07278957072541203 1103 9 0.21246638406512375 1103 10-11 0.2523119510461953 1103 12-13 0.23239298199005987 1103 14-15 0.42653583676706575 1103 16-17 0.30905322653698875 1103 18-19 0.4015171182117143 1103 20-21 0.3062672145065619 1103 22-23 0.3802000265318597 1103 24-25 0.4141523591377876 1103 26-27 0.3822143316103208 1103 28-29 0.33603025855769175 1103 30-31 0.16666411909086065 1103 32-33 0.27053267887418286 1103 34-35 0.37847500873026263 1103 36-37 0.3387678699863059 1103 38-39 0.3553696406890694 1103 40-41 0.1741051466953749 1103 42-43 0.3036812199749903 1103 44-45 0.05353554163524876 1103 46-47 0.40493254114306154 1103 48-49 0.4266419872383622 1103 50-51 0.44183968414200336 1103 52-53 0.30847330190041333 1103 54-55 0.3691832940867883 1103 56-57 0.4493406135584763 1103 58-59 0.42468374649879337 1103 60-61 0.3148945761736073 1103 62-63 0.6049764116763257 1103 64-65 0.562291581821249 1103 66-67 0.48531600906562744 1103 68-69 0.08700752217087881 1103 70-71 0.3878583554083548 1103 72-73 0.6961313467846324 1103 74-75 0.653766634509438 1103 76-77 0.40722556352587347 1103 78-79 0.527018703567915 1103 80-81 0.3433171307249232 1103 82-83 0.1966730357919566 1103 84-85 0.40307473111648306 1103 86-87 0.5138734009238739 1103 88-89 0.6941193102802963 1103 90-91 0.6390580647652193 1103 92-93 1.0351888654855728 1103 94-95 1.0562326297770568 1103 96-97 1.4653127013207339 1103 98-99 1.6735178975612186 1103 100-101 2.6736354545266288 1104 1 0.2146628814885645 1104 2 0.26387636291026695 1104 3 0.16413832071388867 1104 4 0.012218830550530413 1104 5 0.02634186505990499 1104 6 0.04042429782268897 1104 7 0.14529826151488834 1104 8 0.12098077427672393 1104 9 0.08441391495677664 1104 10-11 0.07882272865297324 1104 12-13 0.10167279944239738 1104 14-15 0.28955392250752965 1104 16-17 0.0833362020994386 1104 18-19 0.12738482008612095 1104 20-21 0.1339556113431115 1104 22-23 0.306247429319896 1104 24-25 0.09202735135370688 1104 26-27 0.10583910809619823 1104 28-29 0.08360607544067733 1104 30-31 0.04263824038008579 1104 32-33 0.1285111325567172 1104 34-35 0.1642695180667033 1104 36-37 0.10055685040602214 1104 38-39 -0.026275178675930988 1104 40-41 0.06527100085296667 1104 42-43 0.10957810100764931 1104 44-45 -0.012795890901287521 1104 46-47 0.09245922939755502 1104 48-49 0.13244123373997496 1104 50-51 0.11835969143679392 1104 52-53 0.14817975334113243 1104 54-55 0.23520269644781422 1104 56-57 0.2589749622316262 1104 58-59 0.20313231378433017 1104 60-61 0.2710157497451178 1104 62-63 0.13356731631919416 1104 64-65 0.1872509934341977 1104 66-67 0.36452608121287255 1104 68-69 0.27713131119133294 1104 70-71 0.5577524475184248 1104 72-73 0.4137151256635505 1104 74-75 0.23123485324636306 1104 76-77 0.012859898854895135 1104 78-79 -0.0020498096294296886 1104 80-81 -0.1274186422114063 1104 82-83 -0.131015917049897 1104 84-85 -0.017407058597864733 1104 86-87 0.07142995778320227 1104 88-89 0.2550341391641062 1104 90-91 0.3051257283864608 1104 92-93 0.48476974868887623 1104 94-95 0.42165099040654397 1104 96-97 0.678429533602845 1104 98-99 0.9762816887167283 1104 100-101 1.4430478236429813 1105 1 0.1905325322593967 1105 2 0.13781122251706535 1105 3 0.11679304411959635 1105 4 0.13255830662122747 1105 5 -0.024457631885837827 1105 6 -0.06503529528512786 1105 7 0.014123441604887432 1105 8 0.052882370704011805 1105 9 0.11281338110327965 1105 10-11 0.15373760297496375 1105 12-13 0.05071930629522825 1105 14-15 0.20116438056067665 1105 16-17 0.1921879893109093 1105 18-19 0.09195820783145336 1105 20-21 0.13001801402607072 1105 22-23 0.16569702122996688 1105 24-25 0.16008511703993378 1105 26-27 0.07539384519045456 1105 28-29 0.0870966560063593 1105 30-31 0.28679707890650974 1105 32-33 0.2365266348409989 1105 34-35 0.05370238427822471 1105 36-37 0.2763039458665517 1105 38-39 0.08425452548025447 1105 40-41 0.035893017176562125 1105 42-43 0.09539440920537601 1105 44-45 0.30080881796033054 1105 46-47 0.15137454004211293 1105 48-49 0.13645262090353327 1105 50-51 0.3108538738025146 1105 52-53 0.21252984659438567 1105 54-55 0.286429960619742 1105 56-57 0.3221936111716204 1105 58-59 0.3429672811884643 1105 60-61 0.3514167387429481 1105 62-63 0.4268167217227443 1105 64-65 0.25056589000008245 1105 66-67 0.3326852277317016 1105 68-69 0.2597917666778713 1105 70-71 0.15201737231694068 1105 72-73 0.2733326162098919 1105 74-75 0.2975024130910029 1105 76-77 0.16226615794495203 1105 78-79 0.4565027544073281 1105 80-81 0.5359467308871722 1105 82-83 0.46165664424914254 1105 84-85 0.7422615501320102 1105 86-87 0.7009771233975606 1105 88-89 0.6971379839359351 1105 90-91 0.40881650401399483 1105 92-93 0.8297073374505786 1105 94-95 0.910384671086863 1105 96-97 0.9476876141257478 1105 98-99 1.2496655841361957 1105 100-101 1.8199770538330817 1106 1 -0.13249484294189173 1106 2 0.022651342139788255 1106 3 -0.12735234350405022 1106 4 -0.016510016360136603 1106 5 -0.13855931120490794 1106 6 -0.07021127872198463 1106 7 -0.1951473198397835 1106 8 -0.10918342179655838 1106 9 0.07011726884194758 1106 10-11 -0.01193987574600186 1106 12-13 -0.08646725915220799 1106 14-15 0.061332312467740735 1106 16-17 0.011891232927197848 1106 18-19 0.13314753380338118 1106 20-21 -0.025825096164865613 1106 22-23 -0.03991604525266723 1106 24-25 -0.059503104722196554 1106 26-27 -0.16872853806236066 1106 28-29 -0.2809157663538926 1106 30-31 -0.27338235518596576 1106 32-33 -0.3130822908548936 1106 34-35 -0.5426514229451485 1106 36-37 -0.16439078613252178 1106 38-39 -0.29360376759543527 1106 40-41 -0.11341685169852411 1106 42-43 -0.052397171194897396 1106 44-45 0.14655300935670112 1106 46-47 0.13266048437153444 1106 48-49 -0.004794215995481466 1106 50-51 0.06271722783978362 1106 52-53 0.26417465910876814 1106 54-55 0.2051325141049034 1106 56-57 -0.04706104721347515 1106 58-59 0.04829279303572065 1106 60-61 0.17781425427089204 1106 62-63 0.24066535296267944 1106 64-65 0.11964801560394989 1106 66-67 0.3505366194961397 1106 68-69 -0.010302551242524771 1106 70-71 0.24155038358908598 1106 72-73 0.48381110712315945 1106 74-75 0.48299815728239537 1106 76-77 0.5078378165600874 1106 78-79 0.6062151997719418 1106 80-81 0.534301918372794 1106 82-83 0.39199940937452027 1106 84-85 0.5840834963017834 1106 86-87 0.6448579607566565 1106 88-89 0.789247484741086 1106 90-91 0.8071371849433682 1106 92-93 0.9140413609150357 1106 94-95 0.7926483011765839 1106 96-97 1.027834842099061 1106 98-99 1.330272899526122 1106 100-101 2.301044456639616 1107 1 0.2344725355362769 1107 2 0.3234316702582518 1107 3 0.14341770712211144 1107 4 0.1749939233452409 1107 5 -0.09371899298329112 1107 6 0.1408198963379732 1107 7 0.17334265008903316 1107 8 0.10603487975267711 1107 9 0.3356115400916124 1107 10-11 0.22895693061843048 1107 12-13 0.283895498260911 1107 14-15 0.410531048716841 1107 16-17 0.4100298648787799 1107 18-19 0.3432689604715762 1107 20-21 0.20924779790082937 1107 22-23 0.35884684919367515 1107 24-25 0.3447411189464873 1107 26-27 0.29163079351999244 1107 28-29 0.25145833436553033 1107 30-31 0.24855438113925743 1107 32-33 0.37660215206696535 1107 34-35 0.33566687479761015 1107 36-37 0.34908795468434306 1107 38-39 0.12693218459519784 1107 40-41 0.15977031285554943 1107 42-43 0.48445334838943666 1107 44-45 0.3308779303722673 1107 46-47 0.22783386947298112 1107 48-49 0.10064524196197056 1107 50-51 0.12362849882299543 1107 52-53 0.1382563461400892 1107 54-55 0.08698703049179102 1107 56-57 0.23978376265291956 1107 58-59 0.19547409561270257 1107 60-61 0.41806776574743054 1107 62-63 0.28033266673070756 1107 64-65 0.1860634546812534 1107 66-67 0.3628772232036468 1107 68-69 0.26826697408958466 1107 70-71 0.3263549209116121 1107 72-73 0.25932022363947027 1107 74-75 0.5079191728508903 1107 76-77 0.31777653225419655 1107 78-79 0.4962498387274472 1107 80-81 0.6747114954730158 1107 82-83 0.6751976127166053 1107 84-85 0.572161605243199 1107 86-87 0.515822141792782 1107 88-89 0.660809282921587 1107 90-91 0.8463248824044491 1107 92-93 1.115525887579416 1107 94-95 1.102491854905029 1107 96-97 1.472614256662652 1107 98-99 1.9030832226961536 1107 100-101 2.531656157754913 1108 1 0.06902335379066216 1108 2 0.06610088768305644 1108 3 0.0792648419765456 1108 4 0.08665667404483912 1108 5 -0.1558460752513895 1108 6 0.014297100901053739 1108 7 0.07152989718638736 1108 8 0.01749474476196866 1108 9 0.1451387651994338 1108 10-11 0.13521759364477504 1108 12-13 0.17208037835854384 1108 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-0.5327049595835085 2316 76-77 -0.5689678928037623 2316 78-79 -0.8675536239968764 2316 80-81 -0.8772587862111685 2316 82-83 -0.9115410209100965 2316 84-85 -1.0603325527654306 2316 86-87 -0.8987776886929417 2316 88-89 -0.8947185922482461 2316 90-91 -1.045407987369245 2316 92-93 -1.0225900954838636 2316 94-95 -1.3625665098521829 2316 96-97 -1.9103432158464386 2316 98-99 -2.2282471334125162 2316 100-101 -2.6813016640785996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 5.0 5 10.0 6 41.0 7 104.0 8 167.0 9 387.0 10 742.0 11 1275.0 12 2140.0 13 3024.0 14 3913.0 15 5723.0 16 7910.0 17 11102.0 18 9396.0 19 5244.0 20 4139.0 21 4032.0 22 4148.0 23 4818.0 24 5670.0 25 6746.0 26 8253.0 27 10464.0 28 13222.0 29 16702.0 30 21278.0 31 26730.0 32 34408.0 33 45166.0 34 61699.0 35 87963.0 36 135554.0 37 228834.0 38 217266.0 39 19543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.63847707724302 22.568281872732417 15.924280701406088 21.86896034861848 2 14.365929697903002 22.423205685130906 41.53888285828252 21.67198175868358 3 17.323081533166043 26.54751876073477 32.59391778119769 23.535481924901497 4 11.434249863319254 19.441806745827392 40.6532694729134 28.470673917939955 5 11.490077923727636 39.38532350454324 36.77896734189978 12.345631229829346 6 27.92613385485348 40.603558362926826 18.770458701577557 12.699849080642137 7 25.62553146733107 35.16907234702919 22.67193743345806 16.53345875218168 8 23.929201853896963 37.25773275664477 21.35077290357533 17.462292485882937 9 25.19817467948241 17.31633431664092 23.083319193945762 34.40217180993091 10-11 22.545873630587913 28.550201771541232 29.958583997388434 18.945340600482428 12-13 23.32312610948984 26.82566584274591 29.91869281382996 19.93251523393429 14-15 20.84339214326325 28.414662098404186 28.497365112723166 22.244580645609396 16-17 18.93774998189166 31.56893621201342 29.634519156637623 19.858794649457295 18-19 18.614975050182423 30.083605950648078 32.71854283936763 18.58287615980187 20-21 19.615706138729784 29.036363050041054 32.86307856790258 18.48485224332658 22-23 19.35846678970451 29.234585541068842 32.149937488837296 19.257010180389354 24-25 19.123990829716202 29.288896187366696 32.748391206569025 18.83872177634807 26-27 19.198984727753338 29.41992451939034 32.747540827448866 18.633549925407454 28-29 18.757445035113527 29.375745743833736 32.9749132394492 18.891895981603536 30-31 19.286143783326818 29.108761269351135 32.87792884060875 18.727166106713288 32-33 19.015194136271944 29.26729197488051 32.62755464005088 19.089959248796664 34-35 19.441459462623893 29.331092860551905 32.58647599826557 18.640971678558635 36-37 19.465521227437755 28.988324338698362 32.2734249962543 19.27272943760958 38-39 18.95387384491126 29.761426361573413 32.299768907834014 18.984930885681315 40-41 19.332908655587342 29.33015023471383 32.581564421530366 18.755376688168457 42-43 19.28456105845707 29.26286876160404 32.191775384502414 19.26079479543648 44-45 19.499604593266874 29.437609580253678 31.915624072877876 19.147161753601573 46-47 19.628148990447727 29.701098307937006 31.255289867367587 19.41546283424768 48-49 19.52049119736303 29.642506670318102 31.385322621134865 19.451679511184008 50-51 19.488491530754903 29.788018332584194 30.897737003422225 19.825753133238674 52-53 19.642754375276585 30.27355028963448 30.76743710694944 19.316258228139493 54-55 20.03615714267287 29.787869496925552 30.595302945060787 19.58067041534079 56-57 20.008969829027517 30.112083173335 30.295647152327344 19.58329984531014 58-59 19.929987706174597 29.921623142158893 30.55883820869339 19.589550942973123 60-61 20.049353904405834 30.019705841204257 30.427515545884543 19.503424708505364 62-63 19.94750070200819 29.779931595131288 30.796231084228083 19.476336618632438 64-65 19.942638737159204 29.624696003167223 30.582850361621038 19.849814898052536 66-67 19.77837378190416 29.8879862833057 30.327101088187113 20.006538846603025 68-69 19.823322150814178 29.52621640903214 30.549312726540272 20.101148713613405 70-71 19.867030222568843 29.676540424261006 30.92983589380278 19.52659345936737 72-73 20.09844000230199 29.307407458856822 30.746748929870556 19.84740360897063 74-75 19.839019191881103 29.87233856772327 30.2594108880338 20.029231352361826 76-77 19.623931980119526 29.739944414842682 30.64417065298172 19.991952952056067 78-79 19.514984277993257 29.646624456873877 30.51418751110066 20.324203754032204 80-81 19.482637328181635 29.684031819079344 30.4228024166942 20.41052843604482 82-83 19.52041134247913 30.001875331159667 30.41062806725791 20.067085259103294 84-85 19.48097951869453 29.726791897176234 30.2673142019693 20.52491438215994 86-87 19.36570211237489 29.716874887753107 30.347938080397054 20.56948491947495 88-89 19.863904673737352 29.3692940129368 30.295597540441126 20.47120377288472 90-91 19.491643574967192 29.162224046955743 30.862186587547104 20.483945790529965 92-93 19.60887820768838 29.093708185258187 30.93029474788238 20.36711885917105 94-95 19.414222537092325 29.037837001140083 30.800844989646 20.747095472121593 96-97 19.775248233072674 28.862012476397148 30.422008626514774 20.94073066401541 98-99 19.489503106699395 28.947522479167947 30.267894528994205 21.295079885138446 100-101 19.279634736546488 28.871223991799237 30.897300912662462 20.951840358991813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 708.0 1 468.0 2 272.0 3 321.5 4 259.5 5 258.0 6 484.5 7 899.0 8 1336.5 9 1463.0 10 1238.5 11 1023.5 12 961.5 13 953.5 14 1031.0 15 1127.5 16 1193.5 17 1338.5 18 1542.5 19 1787.0 20 2139.5 21 2632.5 22 3130.5 23 3782.0 24 4710.0 25 6369.5 26 9498.5 27 13062.0 28 14650.5 29 16947.5 30 20850.5 31 24444.5 32 29386.0 33 35128.0 34 40986.5 35 45229.0 36 48158.0 37 50473.5 38 48400.5 39 44875.0 40 43673.0 41 42702.5 42 40767.5 43 39820.0 44 39257.0 45 37295.0 46 34947.5 47 32381.5 48 31765.5 49 29673.0 50 25514.0 51 22189.0 52 18999.0 53 16527.0 54 14284.0 55 12059.5 56 10004.5 57 8200.0 58 6482.0 59 5069.5 60 3929.0 61 2938.5 62 2074.5 63 1380.0 64 854.0 65 539.5 66 347.5 67 235.5 68 187.5 69 148.0 70 111.5 71 67.5 72 54.0 73 48.0 74 38.5 75 32.5 76 23.0 77 12.0 78 8.0 79 9.5 80 6.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04147553687502666 2 9.922377242829347E-5 3 0.0 4 0.0 5 0.01696726508523818 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003968950897131738 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.9611886214146734E-5 22-23 2.976713172848804E-4 24-25 2.4805943107073365E-4 26-27 0.0014387447002102553 28-29 0.002034087334780016 30-31 9.922377242829347E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009128587063402999 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.922377242829347E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.922377242829347E-5 74-75 9.922377242829347E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.922377242829347E-5 84-85 1.488356586424402E-4 86-87 0.0 88-89 0.0 90-91 0.006499157094053221 92-93 0.002927101286634657 94-95 0.0 96-97 0.0 98-99 9.922377242829347E-5 100-101 0.011113062511968868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1007823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.71503362069795 #Duplication Level Percentage of deduplicated Percentage of total 1 78.56134493500151 43.770479743408586 2 13.252923495827629 14.767741562851485 3 3.8726773601452935 6.472990479678324 4 1.6116354388896625 3.5916929064818346 5 0.7526296882115744 2.0966394191321642 6 0.42151540847515706 1.4090847092901366 7 0.24725997472613861 0.9643268463443818 8 0.1589091170507333 0.7082901439293626 9 0.11378957723358607 0.5705811109128829 >10 0.8011311968630619 9.089786777565594 >50 0.12010189249397644 4.737652073618926 >100 0.08071298087773411 8.444449969967332 >500 0.004116182889666298 1.553882456462228 >1k 0.0010737868407825124 1.2384993577847365 >5k 1.7896447346375208E-4 0.5839024425720487 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5856 0.5810544113400865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4786 0.47488497484181247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3376 0.3349794557179187 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1100 0.10914614967112281 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1064 0.10557409386370423 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1054 0.10458185613942131 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1041 0.1032919470978535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.961188621414673E-5 0.0 12-13 0.0 0.0 0.0 9.922377242829346E-5 0.0 14-15 0.0 0.0 0.0 9.922377242829346E-5 0.0 16-17 0.0 0.0 0.0 9.922377242829346E-5 0.0 18-19 0.0 0.0 0.0 1.4883565864244017E-4 0.0 20-21 0.0 0.0 0.0 3.472832034990271E-4 0.0 22-23 0.0 0.0 0.0 3.968950897131738E-4 0.0 24-25 0.0 0.0 0.0 7.441782932122009E-4 0.0 26-27 0.0 0.0 0.0 0.001289909041567815 0.0 28-29 0.0 0.0 0.0 0.0026790418555639236 0.0 30-31 0.0 0.0 0.0 0.006151873890554195 0.0 32-33 0.0 0.0 0.0 0.012601419098393268 0.0 34-35 0.0 0.0 0.0 0.019348635623517223 0.0 36-37 0.0 0.0 0.0 0.025252450083000685 0.0 38-39 0.0 0.0 0.0 0.03066014568034268 0.0 40-41 0.0 0.0 0.0 0.03487715600854516 0.0 42-43 0.0 0.0 0.0 0.03963989708510324 0.0 44-45 0.0 0.0 0.0 0.044551473820303764 0.0 46-47 0.0 0.0 0.0 0.053630448997492614 0.0 48-49 0.0 0.0 0.0 0.06385049755760684 0.0 50-51 0.0 4.961188621414673E-5 0.0 0.07144111614837129 0.0 52-53 0.0 9.922377242829346E-5 0.0 0.08959906650274899 0.0 54-55 0.0 9.922377242829346E-5 0.0 0.12571651966664782 0.0 56-57 0.0 9.922377242829346E-5 0.0 0.19274217794196002 0.0 58-59 0.0 9.922377242829346E-5 0.0 0.2669615597183236 0.0 60-61 0.0 9.922377242829346E-5 0.0 0.3379561688907675 0.0 62-63 0.0 9.922377242829346E-5 0.0 0.42378473204124134 0.0 64-65 0.0 9.922377242829346E-5 0.0 0.5390331437167042 0.0 66-67 0.0 9.922377242829346E-5 0.0 0.7156514586390665 0.0 68-69 0.0 9.922377242829346E-5 0.0 0.9150416293337222 0.0 70-71 0.0 9.922377242829346E-5 0.0 1.094785493087576 0.0 72-73 0.0 9.922377242829346E-5 0.0 1.2785479196247753 0.0 74-75 0.0 9.922377242829346E-5 0.0 1.4861240515447653 0.0 76-77 0.0 9.922377242829346E-5 0.0 1.7434609053375443 0.0 78-79 0.0 9.922377242829346E-5 0.0 1.9694926589291968 0.0 80-81 0.0 9.922377242829346E-5 0.0 2.2007336605733347 0.0 82-83 0.0 9.922377242829346E-5 0.0 2.4397141164668796 0.0 84-85 0.0 9.922377242829346E-5 0.0 2.7669541179353914 0.0 86-87 0.0 9.922377242829346E-5 0.0 3.1693561270183355 0.0 88-89 0.0 9.922377242829346E-5 0.0 3.6311435638996135 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3355 0.0 41.509636 1 CGTACAC 60 8.2827566E-5 39.58923 3 CACACCG 60 8.2827566E-5 39.58923 5 GTATCAA 5060 0.0 32.783 1 ACCGCCC 85 6.406629E-4 27.941175 8 TCAACGC 6205 0.0 26.796898 4 ATCAACG 6300 0.0 26.392818 3 CAACGCA 6320 0.0 26.234129 5 TATCAAC 6450 0.0 25.852688 2 AACGCAG 6450 0.0 25.773916 6 ACACCGT 95 0.0012268045 24.99876 6 TAGGACG 210 1.2623786E-9 24.884659 4 GAGTACT 4730 0.0 23.498941 12-13 GTACATG 3485 0.0 23.45846 1 TACATGG 3530 0.0 22.74418 2 ACGCAGA 7285 0.0 22.625257 7 ACACCGC 105 0.0021966682 22.617926 6 CGCAGAG 7290 0.0 22.544582 8 ACATGGG 3480 0.0 22.388391 3 GTACTTT 5050 0.0 22.008808 14-15 >>END_MODULE