FastQCFastQC Report
Thu 26 May 2016
SRR937647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314211
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43531.385374795917393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34061.0839849655168023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.7899150570794784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18860.6002336009878712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.2587433285276454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.20241175515815804No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5940.1890449411382797No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5790.18427107898832312No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4750.151172301415291No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.14703495421866197No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA4510.1435341219753605No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4480.14257934954536922No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4440.14130631963871412No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4420.14066980468538656No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.13239511029212853No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.13239511029212853No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4050.12889427804882705No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT4000.1273029906655082No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3850.12252912851555164No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3800.12093784113223281No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.11616397898227625No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA3350.10661625468236313No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC3300.10502496729904427No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.1027971649623979No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGT352.778848E-454.3092545
GACCGTG352.7832345E-454.291926
CGTGCAA405.379113E-447.5054249
CCGTGCA459.6089346E-442.2270478
GGTATCA18250.038.7914431
CTGACCG500.001610587538.0164764
ACCGTGC500.001613116538.0043377
GTATCAA28900.034.3606221
TAGACTC600.003938235331.6803975
CATGGGG8600.030.391084
ATGGGGA4550.029.2434445
AGTATCG650.00582531329.2434445
GTATGGT650.005829860529.2387771
TAGGCAT1153.83979E-628.9255815
GTGGTAC1153.8445687E-628.9209631
CATTCCG1004.9379232E-528.5032549
CGGTATA504.94571E-528.50325434-35
GTACTGG1354.1985186E-728.1558591
ATGGGGT2550.027.9532935
CATGGGT3100.027.5926064
Produced by FastQC (version 0.11.5)