##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 314211 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98689097453622 33.0 31.0 34.0 30.0 34.0 2 32.11896146220215 33.0 31.0 34.0 30.0 34.0 3 32.10660670695806 34.0 31.0 34.0 30.0 34.0 4 35.705637294684145 37.0 35.0 37.0 35.0 37.0 5 35.72575434978406 37.0 35.0 37.0 35.0 37.0 6 35.82273376807305 37.0 35.0 37.0 35.0 37.0 7 35.79391555356114 37.0 35.0 37.0 35.0 37.0 8 35.7915540830843 37.0 35.0 37.0 35.0 37.0 9 37.438020947707116 39.0 37.0 39.0 35.0 39.0 10-11 37.351168482325576 39.0 37.0 39.0 35.0 39.0 12-13 37.25990655960485 39.0 37.0 39.0 34.0 39.0 14-15 38.425487968276094 40.0 38.0 41.0 34.0 41.0 16-17 38.37590186212449 40.0 38.0 41.0 34.0 41.0 18-19 38.43338552755951 40.0 38.0 41.0 34.0 41.0 20-21 38.418586554894645 40.0 38.0 41.0 34.0 41.0 22-23 38.32886340707359 40.0 38.0 41.0 34.0 41.0 24-25 38.33022395778633 40.0 38.0 41.0 34.0 41.0 26-27 38.22006231481393 40.0 38.0 41.0 34.0 41.0 28-29 38.10204925989224 40.0 38.0 41.0 34.0 41.0 30-31 37.80156805458752 40.0 37.0 41.0 33.5 41.0 32-33 37.55453978377587 40.0 36.0 41.0 33.0 41.0 34-35 37.48017574177861 40.0 36.0 41.0 33.0 41.0 36-37 37.30850447629141 40.0 36.0 41.0 33.0 41.0 38-39 37.11543198678595 40.0 35.0 41.0 33.0 41.0 40-41 36.895162804612184 40.0 35.0 41.0 32.5 41.0 42-43 36.55633952980641 39.0 35.0 41.0 31.0 41.0 44-45 36.203773897158285 39.0 35.0 41.0 30.5 41.0 46-47 35.69713027233293 39.0 35.0 41.0 28.5 41.0 48-49 35.266822294572755 39.0 35.0 41.0 25.5 41.0 50-51 34.35152174812467 38.0 34.0 40.0 23.5 40.5 52-53 34.42506309454475 38.5 35.0 40.0 23.0 40.5 54-55 34.72387503938436 39.0 35.0 40.0 23.0 41.0 56-57 34.53954030890071 39.0 35.0 40.0 21.5 41.0 58-59 34.337979892492626 39.0 35.0 40.0 19.5 41.0 60-61 34.05606901095124 38.0 34.5 40.0 16.0 41.0 62-63 33.73316815770295 38.0 34.0 40.0 10.5 41.0 64-65 33.379615926877165 38.0 34.0 40.0 7.0 41.0 66-67 32.96888555779397 37.5 33.5 40.0 2.0 41.0 68-69 32.47120565479885 37.0 33.0 39.0 2.0 41.0 70-71 32.04435077066048 36.0 32.5 39.0 2.0 40.5 72-73 31.591187132213705 36.0 32.0 39.0 2.0 40.0 74-75 31.066826113662476 35.0 31.0 38.0 2.0 40.0 76-77 30.57773597996251 35.0 31.0 37.0 2.0 39.0 78-79 30.031200371724733 35.0 30.5 37.0 2.0 39.0 80-81 29.454341191110434 34.5 29.5 36.0 2.0 39.0 82-83 28.940947325205038 34.0 29.0 36.0 2.0 37.5 84-85 28.43889138190579 34.0 29.0 35.0 2.0 37.0 86-87 27.936358052391547 34.0 27.5 35.0 2.0 36.5 88-89 27.510938509472936 34.0 26.5 35.0 2.0 36.0 90-91 27.014353412197536 34.0 26.0 35.0 2.0 36.0 92-93 26.487646836043297 33.0 25.0 35.0 2.0 35.0 94-95 25.84867970885806 33.0 23.5 35.0 2.0 35.0 96-97 25.138933710150184 32.5 19.0 35.0 2.0 35.0 98-99 24.304402137417213 32.0 2.0 34.0 2.0 35.0 100-101 21.7436945237436 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14853476418100442 1101 2 0.12321365881570046 1101 3 0.21688966298479784 1101 4 0.04171546202166354 1101 5 -0.17551144641043237 1101 6 0.03135601574408042 1101 7 0.08459873537911733 1101 8 0.1314759999937678 1101 9 0.19382333950785124 1101 10-11 0.36001530358749534 1101 12-13 0.21723125280747269 1101 14-15 0.200526368807445 1101 16-17 0.2870647713109875 1101 18-19 0.08073015831926966 1101 20-21 0.03290617545011543 1101 22-23 -0.019582454578262798 1101 24-25 0.04162608403190404 1101 26-27 0.18650162498854428 1101 28-29 0.3738568965371485 1101 30-31 0.6400277269344699 1101 32-33 0.8087969318956425 1101 34-35 0.5564182606982158 1101 36-37 0.492011323727354 1101 38-39 0.6358646470974847 1101 40-41 0.8861382512462654 1101 42-43 0.8616112954416053 1101 44-45 1.162163523702901 1101 46-47 1.2336735786094692 1101 48-49 1.7842759618642674 1101 50-51 1.8328472424373885 1101 52-53 1.6103947114030248 1101 54-55 1.8180157052139663 1101 56-57 1.6329636524809104 1101 58-59 1.8722927182348954 1101 60-61 1.7854929805604627 1101 62-63 1.7890109825799243 1101 64-65 1.8517030397784353 1101 66-67 1.9357889834735573 1101 68-69 1.9991471018261322 1101 70-71 2.0644131242222308 1101 72-73 1.8328094804294075 1101 74-75 1.8400023440762432 1101 76-77 2.211341656988022 1101 78-79 2.0880622629267904 1101 80-81 1.9770271414819192 1101 82-83 2.2104637217223413 1101 84-85 2.167876773343874 1101 86-87 2.107033020467881 1101 88-89 2.1571993982238133 1101 90-91 2.2920668546338554 1101 92-93 2.194503965732981 1101 94-95 2.271094713163567 1101 96-97 2.585679844019019 1101 98-99 2.8412237623843666 1101 100-101 2.520752144360035 1102 1 0.2984793756701656 1102 2 0.3327721332578548 1102 3 0.46208668783049944 1102 4 0.10952154948275705 1102 5 0.17037661604670973 1102 6 0.2888545650926062 1102 7 0.2134060361123531 1102 8 0.18177404293304988 1102 9 0.5195710844041983 1102 10-11 0.3636551200141511 1102 12-13 0.5264494835401834 1102 14-15 0.7225815463144372 1102 16-17 0.8288646341584851 1102 18-19 0.7645488005094947 1102 20-21 0.6156381478086104 1102 22-23 0.745245841038134 1102 24-25 0.6717363335439615 1102 26-27 0.5236963287918925 1102 28-29 0.5874481820781128 1102 30-31 0.6825898411930567 1102 32-33 0.9625607130846561 1102 34-35 0.7639616483650471 1102 36-37 0.9566681787149633 1102 38-39 0.8018323635083036 1102 40-41 0.6527284290170172 1102 42-43 0.6116534962117655 1102 44-45 0.7805314089168007 1102 46-47 0.8897346642242852 1102 48-49 1.114177845073641 1102 50-51 0.9964622659115676 1102 52-53 0.8907449777953858 1102 54-55 0.9251819234974548 1102 56-57 0.7473277396254971 1102 58-59 0.886728019179138 1102 60-61 0.9551400766199691 1102 62-63 1.1190790313218102 1102 64-65 1.3879798768307055 1102 66-67 1.379084336113742 1102 68-69 1.5400370105669694 1102 70-71 1.5204372841631475 1102 72-73 1.0670158421955058 1102 74-75 1.2541053139554457 1102 76-77 1.2290607053606557 1102 78-79 1.1468268353802387 1102 80-81 1.0100808947129138 1102 82-83 1.2002590479870463 1102 84-85 1.2426011495637397 1102 86-87 1.3162037753341593 1102 88-89 1.4900711606334802 1102 90-91 1.296482110212267 1102 92-93 1.1397352787044426 1102 94-95 1.398377511074628 1102 96-97 1.607099372425413 1102 98-99 1.5707168256327684 1102 100-101 1.6361158622478875 1103 1 0.21568514119053717 1103 2 0.21138409557768512 1103 3 0.1418789929037061 1103 4 0.3645209820102835 1103 5 0.152557980250549 1103 6 0.031142614122231294 1103 7 0.16878567519985666 1103 8 0.12343787223732505 1103 9 0.24688920947512827 1103 10-11 0.21387075955563262 1103 12-13 0.2123052320363854 1103 14-15 0.3986342077603524 1103 16-17 0.36175533895929846 1103 18-19 0.0878435457143496 1103 20-21 0.21074086032704997 1103 22-23 0.3721695727371497 1103 24-25 0.3444607689088457 1103 26-27 0.4674626436256233 1103 28-29 0.3860919228566928 1103 30-31 0.2875949484453528 1103 32-33 0.37521818669718243 1103 34-35 0.5108036640272857 1103 36-37 0.6194476589101754 1103 38-39 0.5903211843004996 1103 40-41 0.3755748709645843 1103 42-43 0.5220893150745525 1103 44-45 0.4155245992470782 1103 46-47 0.5399049059675534 1103 48-49 0.5523261823792822 1103 50-51 0.04519963964893492 1103 52-53 0.37897843597266245 1103 54-55 0.49469446463920264 1103 56-57 0.756932211308623 1103 58-59 0.6900477331730102 1103 60-61 0.90586927875367 1103 62-63 0.5715191629754273 1103 64-65 0.9023681414998705 1103 66-67 0.8426213920665333 1103 68-69 0.7801970378056495 1103 70-71 0.9398221439974499 1103 72-73 1.2284560873436128 1103 74-75 0.8902050756170041 1103 76-77 1.0112420695644886 1103 78-79 1.0840076321115966 1103 80-81 1.1524966250499915 1103 82-83 1.08037764973486 1103 84-85 1.1399567278181983 1103 86-87 1.1235894296299236 1103 88-89 1.330001361518736 1103 90-91 1.0947059213574306 1103 92-93 0.729679666401637 1103 94-95 0.9835182442490691 1103 96-97 1.4751733708364902 1103 98-99 1.2869664000284118 1103 100-101 1.203619550818992 1104 1 0.1121497876551878 1104 2 0.13839615162199692 1104 3 0.049503626334789885 1104 4 -0.00856471365605671 1104 5 -0.1872106740342403 1104 6 0.10836646553962481 1104 7 -0.10785166595361062 1104 8 0.02413842616368811 1104 9 0.014321597875259329 1104 10-11 0.19476844609847888 1104 12-13 0.22338375924394427 1104 14-15 0.16480396989786072 1104 16-17 0.2567944396435635 1104 18-19 0.1185538748117736 1104 20-21 0.17742381894549908 1104 22-23 0.3141294945357842 1104 24-25 0.15119749096814417 1104 26-27 0.4154505875813115 1104 28-29 0.30670938782898816 1104 30-31 0.48138771481875864 1104 32-33 0.4831116373325699 1104 34-35 0.3964341625610075 1104 36-37 0.5375398713572253 1104 38-39 0.4703716893510048 1104 40-41 0.6322953010721051 1104 42-43 0.36641971585657274 1104 44-45 0.7315070039391571 1104 46-47 0.4226028531816368 1104 48-49 0.032572190851098526 1104 50-51 0.14189237343844496 1104 52-53 0.3718099771913046 1104 54-55 0.47779156467179007 1104 56-57 0.5549120092884223 1104 58-59 0.4241630801915832 1104 60-61 0.6059344465607666 1104 62-63 0.39242988307969995 1104 64-65 0.42297746049629126 1104 66-67 0.5317302223043896 1104 68-69 0.5575675167890282 1104 70-71 0.44100331050119834 1104 72-73 0.5285538390542719 1104 74-75 0.6372725242973551 1104 76-77 0.6056132002259424 1104 78-79 0.40475543269810643 1104 80-81 0.6713138293510674 1104 82-83 0.48001922679579323 1104 84-85 0.4611525570785382 1104 86-87 0.6914454087762287 1104 88-89 1.2361597192899971 1104 90-91 1.1805645072160118 1104 92-93 1.3057223513152891 1104 94-95 1.188878296383315 1104 96-97 1.147053462071419 1104 98-99 1.0407542788162871 1104 100-101 0.798031411359883 1105 1 0.16314481253080615 1105 2 0.30812011246295157 1105 3 0.277179761786563 1105 4 0.2752099613559764 1105 5 0.35367361420200183 1105 6 0.1854166982470602 1105 7 0.20367938845178202 1105 8 0.3505146097618237 1105 9 0.2644981345467414 1105 10-11 0.313998906698707 1105 12-13 0.38175721454806677 1105 14-15 0.4228163274648651 1105 16-17 0.40910947735820713 1105 18-19 0.6522563302763942 1105 20-21 0.4514087330844774 1105 22-23 0.7003355609777628 1105 24-25 0.501670930223483 1105 26-27 0.548214188929407 1105 28-29 0.10812569339792333 1105 30-31 0.3496030895579665 1105 32-33 0.37805626901287326 1105 34-35 0.19907820043615487 1105 36-37 0.24766264458784804 1105 38-39 0.4241030326345836 1105 40-41 0.40144551773074966 1105 42-43 0.21817705817722555 1105 44-45 0.4538669782610967 1105 46-47 0.435449908228982 1105 48-49 0.7718836978323225 1105 50-51 0.500939877851863 1105 52-53 0.5101144227056693 1105 54-55 0.653020259905297 1105 56-57 0.3739418600346909 1105 58-59 0.5736976653304069 1105 60-61 0.6201576852062516 1105 62-63 0.776818424244837 1105 64-65 0.6508341448166206 1105 66-67 0.6596310407925969 1105 68-69 0.5770988921701914 1105 70-71 0.5848847099555421 1105 72-73 0.5766921796095623 1105 74-75 0.7723511633601063 1105 76-77 0.7720591960532595 1105 78-79 0.30626000660277697 1105 80-81 0.4506309534082007 1105 82-83 0.15944066797698397 1105 84-85 0.3356751056261267 1105 86-87 0.5024187677876206 1105 88-89 0.6488502986512579 1105 90-91 0.48330082562397436 1105 92-93 0.9563041198917617 1105 94-95 0.6986961426359244 1105 96-97 0.9505841955010439 1105 98-99 1.193490597224251 1105 100-101 0.8740665582062981 1106 1 -0.14608848864392598 1106 2 -0.19564244357813365 1106 3 0.0857377323898234 1106 4 -0.03959623579515181 1106 5 -0.05203548289804871 1106 6 0.011612714364190424 1106 7 0.13822339843986242 1106 8 0.27268701768419135 1106 9 0.19500151119959952 1106 10-11 -0.05143438312593673 1106 12-13 -0.14841700938928426 1106 14-15 -0.11791915860289492 1106 16-17 -0.3122854491499325 1106 18-19 -0.224510028046069 1106 20-21 -0.029023705483140816 1106 22-23 0.24351131717775587 1106 24-25 -0.011114950260029843 1106 26-27 -0.1560874965595005 1106 28-29 0.1305421283335093 1106 30-31 0.33149401515810695 1106 32-33 -0.19375577343647166 1106 34-35 -0.1092130429666085 1106 36-37 0.018850431887088348 1106 38-39 0.1794971646361816 1106 40-41 0.11385117007738188 1106 42-43 0.17213540333357003 1106 44-45 0.0827529665704958 1106 46-47 0.686667205771677 1106 48-49 1.039042201889977 1106 50-51 0.8268805168621398 1106 52-53 0.998486251773258 1106 54-55 0.8394682481012055 1106 56-57 1.0031059256382306 1106 58-59 0.820448701026443 1106 60-61 0.959326858351929 1106 62-63 1.2650994525633195 1106 64-65 1.4850149518082745 1106 66-67 1.562179136814386 1106 68-69 1.5045604437279891 1106 70-71 1.4109785752299295 1106 72-73 1.7990575391237762 1106 74-75 1.9823959962299789 1106 76-77 2.4017056406364254 1106 78-79 1.9296695747317116 1106 80-81 1.5531620242895343 1106 82-83 1.03659860452996 1106 84-85 1.0335696632562268 1106 86-87 1.70014874905997 1106 88-89 1.8265303395115957 1106 90-91 1.7943160914999154 1106 92-93 1.7079413708990856 1106 94-95 1.7620977656993162 1106 96-97 2.123769599280628 1106 98-99 1.9691446463701539 1106 100-101 2.1198301357558087 1107 1 0.17369277941019234 1107 2 0.08150759202627 1107 3 0.11904464233084155 1107 4 0.07726681489745602 1107 5 0.30103925947387467 1107 6 0.13570413034452145 1107 7 0.09359025813164834 1107 8 0.13710814385812853 1107 9 -0.100756862552565 1107 10-11 0.3130337677292374 1107 12-13 0.3125256339352305 1107 14-15 0.3628880536396437 1107 16-17 0.6468501028526461 1107 18-19 0.37582048828492987 1107 20-21 0.2611656024479032 1107 22-23 0.2534603961657851 1107 24-25 0.328483862030879 1107 26-27 0.4375923862818638 1107 28-29 0.3318480709096576 1107 30-31 0.5601616044042359 1107 32-33 0.6461117513186636 1107 34-35 0.4320880627851764 1107 36-37 0.5665065874178623 1107 38-39 0.5339759296336908 1107 40-41 -0.07024373916498661 1107 42-43 -0.07430751542121783 1107 44-45 -0.07613671352731188 1107 46-47 -0.11688382944308984 1107 48-49 -0.322067043162356 1107 50-51 -0.4455872040531048 1107 52-53 -0.20234272617888394 1107 54-55 -0.3745679474677317 1107 56-57 -0.6575438211002691 1107 58-59 -0.4923326073345393 1107 60-61 0.012014654775370559 1107 62-63 -0.14987320594345022 1107 64-65 -0.1193731227325614 1107 66-67 0.09775666707813002 1107 68-69 0.2478862978977432 1107 70-71 -0.028500660014401547 1107 72-73 -0.058807002923007445 1107 74-75 -0.23409891838663555 1107 76-77 -0.41448159820864916 1107 78-79 -0.2597122522443307 1107 80-81 -0.3002418908510691 1107 82-83 -0.5323530137908534 1107 84-85 -0.13053975645921057 1107 86-87 -0.18701216939923526 1107 88-89 -0.3648937021925782 1107 90-91 -0.3474095799453494 1107 92-93 -0.126175707121579 1107 94-95 0.042781370208309966 1107 96-97 -0.03437440372895395 1107 98-99 -0.3520607977443717 1107 100-101 -0.1814368435130227 1108 1 -0.0851541339134414 1108 2 -0.25411649859938024 1108 3 0.01732462570227966 1108 4 0.12651593150523155 1108 5 -0.11043964304119669 1108 6 0.02044742695645141 1108 7 0.026517404255116617 1108 8 -0.038401105778120836 1108 9 0.15560875790055917 1108 10-11 0.32306936323953295 1108 12-13 0.25995080541725457 1108 14-15 -0.12221769360624535 1108 16-17 0.2631590822748038 1108 18-19 0.2925932486258134 1108 20-21 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-1.1197260828970919 2316 90-91 -1.104374114124873 2316 92-93 -1.1065008355012438 2316 94-95 -0.7621215817731937 2316 96-97 -0.6061068535032277 2316 98-99 -1.270388308530265 2316 100-101 -1.1115112186318932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1022.0 3 155.0 4 118.0 5 130.0 6 155.0 7 181.0 8 237.0 9 282.0 10 433.0 11 653.0 12 1124.0 13 1575.0 14 1913.0 15 2835.0 16 3961.0 17 5484.0 18 4598.0 19 2523.0 20 2295.0 21 2149.0 22 2335.0 23 2641.0 24 2755.0 25 3280.0 26 3380.0 27 3931.0 28 4600.0 29 5470.0 30 6581.0 31 7894.0 32 9995.0 33 13326.0 34 18240.0 35 27190.0 36 38385.0 37 63362.0 38 61187.0 39 7836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.163331663438555 23.331089079845434 15.698819054669377 19.80676020204663 2 14.058268284202233 23.759972693719874 41.496294831265686 20.6854641908122 3 17.15815128623928 29.44569211923234 32.59780777868518 20.7983488158432 4 10.511725242368463 20.661054203126945 41.19960825472375 27.62761229978084 5 10.07602561198544 41.42518511901533 35.87911193917951 12.619677329819714 6 24.502587089102345 42.7438765848511 19.204075581895797 13.549460744150757 7 23.702877181796445 38.18442655614888 22.532238782179007 15.580457479875667 8 23.930779212084307 37.08725606781801 21.14498681706162 17.83697790303606 9 25.96189097771926 18.08122316125721 23.120132364614783 32.83675349640875 10-11 21.886622211665532 30.49014747306317 28.903190015638426 18.720040299632874 12-13 23.201818425264122 29.99764089289016 28.972704493142647 17.827836188703063 14-15 20.81262453176058 30.99338487287798 28.487765999840597 19.70622459552084 16-17 17.13410337912587 34.33930262289542 29.484060817251745 19.042533180726963 18-19 16.92127696201886 32.15772661485198 34.155609821411495 16.765386601717662 20-21 18.010994773577476 30.871118237648414 34.31443649793031 16.8034504908438 22-23 18.05476967313844 31.00140104018069 33.73478420952803 17.209045077152844 24-25 17.38740260899504 31.993981204014233 34.06852118692697 16.55009500006376 26-27 17.141591143102143 31.827962473581362 34.513976773913846 16.516469609402645 28-29 16.584787342232197 31.872204493214273 34.75020164311241 16.79280652144112 30-31 17.31630024484799 31.77078019791879 34.32207712711691 16.590842430116304 32-33 16.920739726420464 31.932065810847217 34.24861273399403 16.898581728738286 34-35 17.28402933754597 32.09276301211983 33.91846891294541 16.704738737388794 36-37 17.601502069649236 31.568486502252945 33.684836332744126 17.14517509535369 38-39 16.535525936220502 32.76074480072293 33.665050052355355 17.03867921070121 40-41 16.847116652029843 32.25642439668926 34.0004112123912 16.896047738889695 42-43 17.026309329881954 32.019248241103504 33.49984538925248 17.45459703976206 44-45 17.466486012592654 32.20004782019606 32.93759464413804 17.395871523073243 46-47 17.755415316390927 32.38462016670359 32.55310907464999 17.306855442255486 48-49 17.32324882644075 32.45654443585473 32.705841097287866 17.514365640416663 50-51 17.16954996341749 32.472993501225 32.27453937122718 18.082917164130333 52-53 17.417499286553568 33.08781210292123 32.05120233659685 17.443486273928354 54-55 17.885530944324284 32.40973476293471 31.666988098520722 18.037746194220283 56-57 17.88920056100982 33.01224021420375 31.27183475710825 17.826724467678183 58-59 17.832211299391805 32.43299672315088 31.632910020528122 18.101881956929198 60-61 17.929686939697284 32.63139273026898 31.649961510938702 17.78895881909504 62-63 17.705992419823215 32.24125235485938 32.090477602139536 17.962277623177872 64-65 17.655927757005475 32.130983459720326 32.16779984635932 18.045288936914883 66-67 17.774200745864277 32.397921779874416 31.385905077614506 18.441972396646797 68-69 17.63590140025662 31.69202323931876 31.788295862986843 18.88377949743778 70-71 17.680210513229596 32.1362598199927 32.058162089449 18.125367577328706 72-73 17.83654903560807 31.58969275247752 32.000248628558495 18.573509583355914 74-75 17.378062385648622 32.538425250058964 31.53707375225515 18.546438612037257 76-77 17.477806289147793 32.07449436590536 31.808966737325278 18.638732607621566 78-79 17.483073647166826 32.01104835007905 31.242190289182435 19.263687713571684 80-81 17.546021070079533 31.712541638111023 31.288111821239024 19.453325470570423 82-83 17.664440654734392 31.875109573976378 31.49690005259551 18.96354971869372 84-85 17.756669747872376 31.4208077008893 31.21665126063813 19.605871290600195 86-87 17.518008542104926 31.41454707719768 31.355102951488494 19.712341429208898 88-89 18.10300314274968 30.82667703625318 31.49666282057003 19.573657000427108 90-91 17.547824451630486 30.683192791150226 32.05573024825843 19.71325250896086 92-93 18.04118125169331 30.644492963807036 31.829888281511465 19.484437502988193 94-95 17.818152840863814 30.58889154514304 31.843334130209577 19.74962148378357 96-97 18.63007585899152 30.679543571109836 30.778510868872317 19.911869701026326 98-99 18.390654384343225 30.799572881139838 30.608963121155135 20.20080961336181 100-101 18.713756886092046 29.949500663710115 31.51260571157214 19.824136738625697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1045.0 1 651.5 2 344.5 3 421.5 4 358.5 5 386.5 6 703.5 7 1360.0 8 1967.0 9 2082.0 10 1689.5 11 1278.0 12 1200.5 13 1192.0 14 1133.0 15 1018.5 16 959.0 17 1005.0 18 1065.5 19 1129.5 20 1250.5 21 1431.5 22 1599.0 23 1880.0 24 2213.5 25 2793.0 26 4147.0 27 5523.5 28 5897.0 29 6344.0 30 7487.0 31 8816.5 32 10258.0 33 11886.0 34 14103.0 35 15573.0 36 16983.5 37 18375.0 38 17222.5 39 15340.5 40 13888.5 41 12358.0 42 11167.5 43 10346.0 44 9847.0 45 9379.5 46 8424.0 47 7536.0 48 7427.5 49 6614.0 50 5240.0 51 4210.0 52 3353.5 53 2825.0 54 2437.5 55 2052.0 56 1728.0 57 1422.0 58 1088.0 59 858.0 60 670.0 61 513.5 62 382.0 63 262.5 64 178.0 65 116.0 66 81.0 67 66.5 68 46.0 69 27.5 70 16.0 71 11.0 72 11.0 73 8.5 74 5.0 75 3.5 76 4.0 77 3.0 78 1.5 79 2.0 80 3.0 81 2.0 82 0.5 83 0.5 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2603346159109643 2 0.2323279579645525 3 0.2351922752545264 4 0.23614704768451772 5 0.24314871217112066 6 0.17122252244510855 7 0.17058600749178102 8 0.17440509721174624 9 0.16931297758512592 10-11 0.17838331567004337 12-13 0.16963123506178968 14-15 0.17058600749178102 16-17 0.1750416121650738 18-19 0.1683582051551346 20-21 0.16437998669683748 22-23 0.1640617292201737 24-25 0.16772169020180708 26-27 0.1629478280518505 28-29 0.17122252244510855 30-31 0.1740868397350825 32-33 0.17647377081006077 34-35 0.17551899838006946 36-37 0.16247044183685486 38-39 0.15578703482691567 40-41 0.16056089697687223 42-43 0.16549388786516067 44-45 0.17058600749178102 46-47 0.17201816613676796 48-49 0.16819907641680273 50-51 0.1707451362301129 52-53 0.18856755492328403 54-55 0.16247044183685486 56-57 0.15626442104191132 58-59 0.1575374509485664 60-61 0.1545140049202606 62-63 0.15849222337855773 64-65 0.1565826785185751 66-67 0.15308184627527363 68-69 0.16501650165016502 70-71 0.15960612454688092 72-73 0.15594616356524757 74-75 0.1543548761819287 76-77 0.15785570842523017 78-79 0.15881048085522148 80-81 0.15785570842523017 82-83 0.15785570842523017 84-85 0.15308184627527363 86-87 0.1498992715086359 88-89 0.15053578646196344 90-91 0.1545140049202606 92-93 0.15149055889195476 94-95 0.15467313365859248 96-97 0.1498992715086359 98-99 0.15308184627527363 100-101 0.141465448377046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 314211.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.44370356181224 #Duplication Level Percentage of deduplicated Percentage of total 1 83.72239539771597 41.39545299529502 2 8.913793140310831 8.814618912816883 3 2.38759611832402 3.5415478409923926 4 1.0546343826697653 2.085801191312749 5 0.6342067086845355 1.5678764250555393 6 0.43092699162278064 1.2783975858348189 7 0.3379033373892463 1.1695034712494645 8 0.26642270876771335 1.0538340347556683 9 0.20231961096323844 0.9003087782286786 >10 1.7396220414692731 17.528741353507687 >50 0.20032430051169664 6.743873964699032 >100 0.10468560220288663 9.207606950997143 >500 0.002584829684021892 0.8377522876505833 >1k 0.002584829684021892 3.874684207604356 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4353 1.385374795917393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3406 1.0839849655168023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2482 0.7899150570794784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1886 0.6002336009878712 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 813 0.2587433285276454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 0.20241175515815804 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 594 0.1890449411382797 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 579 0.18427107898832312 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 475 0.151172301415291 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 462 0.14703495421866197 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 451 0.1435341219753605 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 448 0.14257934954536922 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 444 0.14130631963871412 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 442 0.14066980468538656 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 416 0.13239511029212853 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.13239511029212853 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 405 0.12889427804882705 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 400 0.1273029906655082 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 385 0.12252912851555164 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 380 0.12093784113223281 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.11616397898227625 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 335 0.10661625468236313 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 330 0.10502496729904427 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.1027971649623979 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.5912873833188527E-4 0.0 22-23 0.0 0.0 0.0 0.0011139011683231968 0.0 24-25 0.0 0.0 0.0 0.0012730299066550821 0.0 26-27 0.0 0.0 0.0 0.0027051885516420496 0.0 28-29 0.0 0.0 0.0 0.00604689205661164 0.0 30-31 0.0 0.0 0.0 0.013685071496542132 0.0 32-33 0.0 0.0 0.0 0.020686735983145084 0.0 34-35 0.0 0.0 0.0 0.02752927173141615 0.0 36-37 0.0 0.0 0.0 0.034053550003023444 0.0 38-39 0.0 0.0 0.0 0.04439691799459598 0.0 40-41 0.0 0.0 0.0 0.07288096215600345 0.0 42-43 0.0 0.0 0.0 0.12364302968387485 0.0 44-45 0.0 0.0 0.0 0.1662895315568201 0.0 46-47 0.0 0.0 0.0 0.26431283436926145 0.0 48-49 0.0 0.0 0.0 0.36026746358338824 0.0 50-51 0.0 0.0 0.0 0.4321936533094004 0.0 52-53 0.0 0.0 0.0 0.5178049145319547 0.0 54-55 0.0 0.0 0.0 0.5889354605663073 0.0 56-57 0.0 0.0 0.0 0.7703422222646565 0.0 58-59 0.0 0.0 0.0 0.9902581386393221 0.0 60-61 0.0 0.0 0.0 1.1976028846857685 0.0 62-63 0.0 0.0 0.0 1.4495036774651429 0.0 64-65 0.0 0.0 0.0 1.78096883941046 0.0 66-67 0.0 0.0 0.0 2.21316249271986 0.0 68-69 0.0 0.0 0.0 2.6935721537438218 0.0 70-71 0.0 0.0 0.0 3.066410787655429 0.0 72-73 0.0 0.0 0.0 3.3830769769358806 0.0 74-75 0.0 0.0 0.0 3.810496768095325 0.0 76-77 0.0 0.0 0.0 4.375722046650181 0.0 78-79 0.0 0.0 0.0 4.898937338285419 0.0 80-81 0.0 0.0 0.0 5.414991836695724 0.0 82-83 0.0 0.0 0.0 5.995811731607105 0.0 84-85 0.0 0.0 0.0 6.842376619532734 0.0 86-87 0.0 0.0 0.0 7.871462170325036 0.0 88-89 0.0 0.0 0.0 8.921393585838816 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACCGT 35 2.778848E-4 54.309254 5 GACCGTG 35 2.7832345E-4 54.29192 6 CGTGCAA 40 5.379113E-4 47.505424 9 CCGTGCA 45 9.6089346E-4 42.227047 8 GGTATCA 1825 0.0 38.791443 1 CTGACCG 50 0.0016105875 38.016476 4 ACCGTGC 50 0.0016131165 38.004337 7 GTATCAA 2890 0.0 34.360622 1 TAGACTC 60 0.0039382353 31.680397 5 CATGGGG 860 0.0 30.39108 4 ATGGGGA 455 0.0 29.243444 5 AGTATCG 65 0.005825313 29.243444 5 GTATGGT 65 0.0058298605 29.238777 1 TAGGCAT 115 3.83979E-6 28.925581 5 GTGGTAC 115 3.8445687E-6 28.920963 1 CATTCCG 100 4.9379232E-5 28.503254 9 CGGTATA 50 4.94571E-5 28.503254 34-35 GTACTGG 135 4.1985186E-7 28.155859 1 ATGGGGT 255 0.0 27.953293 5 CATGGGT 310 0.0 27.592606 4 >>END_MODULE