FastQCFastQC Report
Thu 26 May 2016
SRR937647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314211
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53551.7042687875344913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45341.4429793991935356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34901.110718593556559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18050.5744547453781058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.3793629121832145No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.287068243950721No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.23678356263784525No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7040.22405326357129446No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA6830.21736985656135524No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6190.19700137805487394No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.18681713880163328No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5680.18077024674502165No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5590.17790592945504774No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.17758767197838396No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT5140.16358434300517805No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC5120.1629478280518505No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.1489444990786446No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT4400.14003328973205903No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT4360.13876025982540396No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT3870.12316564346887919No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA3680.11711875141226756No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG3510.11170837430898345No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC3500.11139011683231968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG1350.049.265129
GGTATCA21200.044.8237881
TAGGCAT1650.040.314245
CTGCTCG500.00161308638.004529
GGTACTC756.900611E-638.004528
GTATGGT851.6253478E-533.538741
GGCATTC2150.033.1434757
GTATCAA32300.032.950341
TGGTACT902.4054692E-531.6704357
GACGTCC300.003950379331.66539478-79
AAGCGTC300.003950379331.66539468-69
CGCTGTA452.4118981E-531.66539416-17
ATATCGG300.003950379331.66539450-51
CATGGGG6100.031.1562044
TTAGGCA2200.030.235684
GTCTAGA953.476251E-530.0083431
AGGCATT2400.029.6863066
GCATTCC2350.028.3012378
CCTTTAG2201.8189894E-1228.0759891
ACGGAGG856.392292E-427.94458
Produced by FastQC (version 0.11.5)