##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937647_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 314211 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11548290798222 33.0 31.0 34.0 31.0 34.0 2 32.39110024792257 33.0 31.0 34.0 31.0 34.0 3 32.45037252037643 34.0 31.0 34.0 31.0 34.0 4 36.1206450442537 37.0 35.0 37.0 35.0 37.0 5 36.06016975853805 37.0 35.0 37.0 35.0 37.0 6 36.113617919168966 37.0 35.0 37.0 35.0 37.0 7 36.06036071302405 37.0 35.0 37.0 35.0 37.0 8 36.048658385607126 37.0 35.0 37.0 35.0 37.0 9 37.70529357660935 39.0 37.0 39.0 35.0 39.0 10-11 37.64880923965106 39.0 37.0 39.0 35.0 39.0 12-13 37.55811858910096 39.0 37.0 39.0 35.0 39.0 14-15 38.694585803807 40.0 38.0 41.0 34.5 41.0 16-17 38.67456740852485 40.0 38.0 41.0 34.5 41.0 18-19 38.79206806890911 40.0 38.0 41.0 35.0 41.0 20-21 38.78180585657408 40.0 38.0 41.0 35.0 41.0 22-23 38.7037723058709 40.0 38.0 41.0 35.0 41.0 24-25 38.69059485504963 40.0 38.0 41.0 35.0 41.0 26-27 38.52899643869884 40.0 38.0 41.0 34.0 41.0 28-29 38.349970242925934 40.0 38.0 41.0 34.0 41.0 30-31 38.189622578458426 40.0 37.0 41.0 34.0 41.0 32-33 37.93403159023713 40.0 36.5 41.0 33.5 41.0 34-35 37.724056446146065 40.0 36.0 41.0 33.0 41.0 36-37 37.49015311367202 40.0 35.5 41.0 33.0 41.0 38-39 37.307315784616065 40.0 35.0 41.0 33.0 41.0 40-41 37.06502318505717 40.0 35.0 41.0 33.0 41.0 42-43 36.72686188580285 40.0 35.0 41.0 31.5 41.0 44-45 36.23942509969416 40.0 35.0 41.0 30.5 41.0 46-47 35.609210053117174 39.0 35.0 41.0 28.0 41.0 48-49 35.082939489705964 39.0 35.0 41.0 24.0 41.0 50-51 34.768665005362635 39.0 35.0 41.0 22.5 41.0 52-53 34.54001292125355 39.0 35.0 41.0 20.0 41.0 54-55 34.313340398649316 39.0 35.0 40.0 18.5 41.0 56-57 34.05447613228054 38.5 34.5 40.0 13.0 41.0 58-59 33.8166327722454 38.0 34.0 40.0 8.0 41.0 60-61 33.531792330631326 38.0 34.0 40.0 3.5 41.0 62-63 33.190995859470235 38.0 33.5 40.0 2.0 41.0 64-65 32.823798339332484 37.5 33.0 40.0 2.0 41.0 66-67 32.40775147910162 37.0 33.0 39.5 2.0 41.0 68-69 31.953018831294894 36.0 32.0 39.0 2.0 40.0 70-71 31.545555375209652 36.0 31.5 39.0 2.0 40.0 72-73 31.05644296348632 35.5 31.0 38.5 2.0 40.0 74-75 30.573114881401352 35.0 31.0 37.5 2.0 39.5 76-77 29.25747348119576 34.0 29.0 36.0 2.0 39.0 78-79 29.785230307022985 35.0 30.0 36.0 2.0 39.0 80-81 29.536125406176104 35.0 30.0 36.0 2.0 38.5 82-83 29.119079217468517 34.0 29.5 36.0 2.0 37.5 84-85 28.700677251910342 34.0 29.0 35.0 2.0 37.0 86-87 28.315191065876114 34.0 29.0 35.0 2.0 36.5 88-89 27.847860195855652 34.0 28.5 35.0 2.0 36.0 90-91 27.318015600981507 34.0 26.5 35.0 2.0 36.0 92-93 26.904471835804603 33.5 26.0 35.0 2.0 35.0 94-95 26.455483735451654 33.0 25.0 35.0 2.0 35.0 96-97 25.922119212885608 33.0 24.5 35.0 2.0 35.0 98-99 25.13270222875711 32.5 18.5 34.5 2.0 35.0 100-101 22.64262231430472 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31876062302983144 1101 2 0.265040661405628 1101 3 0.2597044698779314 1101 4 0.08072306703881083 1101 5 0.14515594522485742 1101 6 0.13102445540376095 1101 7 0.11248583174015181 1101 8 -0.04072553922350153 1101 9 0.18196106700501247 1101 10-11 0.2791533083452862 1101 12-13 0.31396150985973037 1101 14-15 0.3831287290297851 1101 16-17 0.3947778770609247 1101 18-19 0.26667823724092443 1101 20-21 0.3286734169278489 1101 22-23 0.267500407335028 1101 24-25 0.20299562505991986 1101 26-27 0.05206484938098299 1101 28-29 0.08315805766520867 1101 30-31 0.37251040859015205 1101 32-33 0.28276300535210197 1101 34-35 0.3626075577344565 1101 36-37 0.2136733558805659 1101 38-39 0.4792683437161003 1101 40-41 0.589609844292049 1101 42-43 0.86101577179641 1101 44-45 1.1774933858343957 1101 46-47 1.419284874101912 1101 48-49 1.234977669768405 1101 50-51 1.1093675673488406 1101 52-53 1.1618169437547508 1101 54-55 1.2194594236534329 1101 56-57 1.446184913863398 1101 58-59 1.4461717324327097 1101 60-61 1.430187873657765 1101 62-63 1.4355512725271424 1101 64-65 1.6191396794788915 1101 66-67 1.9284071795322077 1101 68-69 2.0268211644077887 1101 70-71 1.8102358192224735 1101 72-73 1.3858220244335797 1101 74-75 2.034660813769854 1101 76-77 1.865127881542275 1101 78-79 1.8207605649172152 1101 80-81 1.6618884513120804 1101 82-83 1.76632228377089 1101 84-85 1.747142326196876 1101 86-87 1.91654436748874 1101 88-89 1.5854685134679158 1101 90-91 1.4728262490489676 1101 92-93 1.5552948170095995 1101 94-95 1.6543339635725935 1101 96-97 1.6589720184222578 1101 98-99 1.7806723760785594 1101 100-101 1.791160235595033 1102 1 0.35405101707952724 1102 2 0.3370562756905926 1102 3 0.33645712061380806 1102 4 0.04563840174366618 1102 5 0.18332654183824815 1102 6 0.14746693047892734 1102 7 0.2785036615655514 1102 8 0.2585517725874382 1102 9 0.36450049834963494 1102 10-11 0.3948203442686946 1102 12-13 0.38152230534425513 1102 14-15 0.6010983444752682 1102 16-17 0.5701801031515501 1102 18-19 0.5006287568379051 1102 20-21 0.3685346818959374 1102 22-23 0.5267080878751997 1102 24-25 0.35947080573196644 1102 26-27 0.29275694201167113 1102 28-29 0.24232353743521173 1102 30-31 0.491582519156168 1102 32-33 0.5417675946858509 1102 34-35 0.201139498442366 1102 36-37 0.08402203974404898 1102 38-39 0.48875560435860876 1102 40-41 0.38773454756788794 1102 42-43 0.5587764934295762 1102 44-45 0.5914671158549609 1102 46-47 0.7246918477791766 1102 48-49 0.766008423579656 1102 50-51 0.7576953618310895 1102 52-53 0.6689963497789151 1102 54-55 0.7899637228568892 1102 56-57 0.7585919402916304 1102 58-59 0.7229929594201039 1102 60-61 0.8829309237184262 1102 62-63 0.9113939691563573 1102 64-65 0.8910418791940415 1102 66-67 1.0262180145799462 1102 68-69 1.0468586175913153 1102 70-71 0.8559313406657374 1102 72-73 0.6709277901138009 1102 74-75 0.6409698289093839 1102 76-77 0.8598000343090639 1102 78-79 1.113586433989326 1102 80-81 0.9297789403135042 1102 82-83 0.8968308030513654 1102 84-85 1.1022327940979189 1102 86-87 0.9528159294447427 1102 88-89 0.8986931798180748 1102 90-91 1.1019052172401338 1102 92-93 1.0119308881178952 1102 94-95 0.7039831696706074 1102 96-97 0.6878452578588714 1102 98-99 0.3017120975534766 1102 100-101 0.3432597239106947 1103 1 0.3493126218916913 1103 2 0.30764985190214134 1103 3 0.18213298033461456 1103 4 0.15825898964515517 1103 5 0.019746925449645403 1103 6 0.09161628923502718 1103 7 0.06432819907548293 1103 8 -0.00860859513472434 1103 9 0.2690289287207577 1103 10-11 0.3705069859665713 1103 12-13 0.2595440962873923 1103 14-15 0.3580896053991083 1103 16-17 0.3258491532026184 1103 18-19 0.11619452689806309 1103 20-21 0.18797061425322426 1103 22-23 0.05957609377691142 1103 24-25 0.08265489380369928 1103 26-27 0.2601669976239762 1103 28-29 0.22106885578727997 1103 30-31 0.24450500241572826 1103 32-33 0.17627559604778753 1103 34-35 0.3764430962178764 1103 36-37 0.4009866294614497 1103 38-39 0.44435939507476263 1103 40-41 0.7689739074589284 1103 42-43 0.6373175216897025 1103 44-45 0.8460806670977234 1103 46-47 0.6762382102805944 1103 48-49 0.4485926932425812 1103 50-51 0.6809815949487827 1103 52-53 0.8570794277496319 1103 54-55 0.5389394350348482 1103 56-57 0.4941469283747111 1103 58-59 0.7632154086313108 1103 60-61 0.5317136952540125 1103 62-63 0.8459048078806788 1103 64-65 0.5991510608987269 1103 66-67 0.6995695070325709 1103 68-69 0.6922252048118303 1103 70-71 0.7596596348434694 1103 72-73 0.7180705661891622 1103 74-75 0.35061969839070883 1103 76-77 0.5668991150036469 1103 78-79 0.6569214697400945 1103 80-81 0.5202964752130583 1103 82-83 0.622809693075201 1103 84-85 0.38384384846177966 1103 86-87 0.24007900968535623 1103 88-89 0.5293083199837767 1103 90-91 0.7138278139941931 1103 92-93 0.7866755255029823 1103 94-95 0.532481637494918 1103 96-97 0.8651179851316009 1103 98-99 0.8344495847853466 1103 100-101 1.0072649446574893 1104 1 0.3364419799887912 1104 2 0.2837821425505638 1104 3 0.10246533615083564 1104 4 0.09757146928021143 1104 5 -0.03654176793582309 1104 6 0.13454558216432133 1104 7 0.05031712054827153 1104 8 0.03855660525628224 1104 9 0.12460077622809251 1104 10-11 0.17247831213144593 1104 12-13 0.22044341202541062 1104 14-15 0.18246236525574488 1104 16-17 0.14818541908727667 1104 18-19 0.22376213849792492 1104 20-21 0.29105793911473654 1104 22-23 0.38602607748496354 1104 24-25 0.18961155721196832 1104 26-27 0.11533154633691112 1104 28-29 0.22375702783028828 1104 30-31 0.3939022002000243 1104 32-33 0.5496871307496392 1104 34-35 0.5377741486779613 1104 36-37 0.5492026607419902 1104 38-39 0.3403352829861319 1104 40-41 0.5545310922096647 1104 42-43 0.6866821355704502 1104 44-45 1.0395068645561807 1104 46-47 0.7111029870808565 1104 48-49 0.7496679620597817 1104 50-51 0.8990086395887289 1104 52-53 1.2550510797535352 1104 54-55 1.1340437035262099 1104 56-57 1.0345916986581827 1104 58-59 1.4442597227401492 1104 60-61 1.7212298242862687 1104 62-63 1.5901211650002622 1104 64-65 1.5192797673177552 1104 66-67 1.6100859619038559 1104 68-69 1.5147243902142442 1104 70-71 1.0119812379715292 1104 72-73 1.1536358969157803 1104 74-75 1.2871218163444418 1104 76-77 1.487231470819875 1104 78-79 1.866232320179975 1104 80-81 1.4827842562492286 1104 82-83 1.6217099863303304 1104 84-85 1.449133845203388 1104 86-87 1.5735752625364476 1104 88-89 1.5523207018671243 1104 90-91 1.623326021879496 1104 92-93 1.4384350562947716 1104 94-95 1.8678921946796692 1104 96-97 1.8112323546330664 1104 98-99 1.961404618998447 1104 100-101 1.3227178609168568 1105 1 0.11199165098863517 1105 2 0.08966402346855062 1105 3 0.07917051990946788 1105 4 -0.12135807653907449 1105 5 0.026425653023892437 1105 6 -0.06769322380582565 1105 7 0.05861434410789457 1105 8 0.13881338586934788 1105 9 0.009779720217821364 1105 10-11 0.0506479478239541 1105 12-13 -0.07345002402016831 1105 14-15 0.013893917173575687 1105 16-17 -0.025711226715969815 1105 18-19 0.1406254028214704 1105 20-21 -0.22972122955803087 1105 22-23 -0.13864492235455117 1105 24-25 -0.24208881191015763 1105 26-27 -0.5217491790683155 1105 28-29 -0.2788067661032727 1105 30-31 0.0022315213362773534 1105 32-33 -0.04817035601011099 1105 34-35 -0.1078324955203982 1105 36-37 -0.2874688205611662 1105 38-39 -0.17983403173060708 1105 40-41 -0.23695101339136926 1105 42-43 0.06607147917500811 1105 44-45 -0.03144081639391061 1105 46-47 -0.09563349181498637 1105 48-49 -0.15663118735443504 1105 50-51 -0.04539033328881459 1105 52-53 0.33161583661439664 1105 54-55 -0.0529006071783229 1105 56-57 0.13771318798574583 1105 58-59 0.2746280424320062 1105 60-61 0.43532066042813966 1105 62-63 0.1437602277510237 1105 64-65 0.04244218105552022 1105 66-67 0.4823977898608476 1105 68-69 0.8106520101789378 1105 70-71 0.6487369754133496 1105 72-73 0.491186049379273 1105 74-75 0.46474980543126776 1105 76-77 0.49030481378248325 1105 78-79 0.7821928401607181 1105 80-81 0.7010517895504655 1105 82-83 0.7111859933918012 1105 84-85 0.5785031278225539 1105 86-87 0.3172801253193036 1105 88-89 0.36857411223464354 1105 90-91 0.7122523579411251 1105 92-93 1.003059514648168 1105 94-95 0.4707448668029208 1105 96-97 0.4430691384558898 1105 98-99 0.35215650473107374 1105 100-101 0.6905681257218639 1106 1 -0.1064522511735575 1106 2 -0.025664277989932316 1106 3 -0.09696023316924851 1106 4 0.06548716493416862 1106 5 -0.10793675546597825 1106 6 -0.17248863836800155 1106 7 0.03561905439675428 1106 8 -0.13779974618899615 1106 9 -0.020059675957199374 1106 10-11 -0.1852069979793498 1106 12-13 -0.26398972097266693 1106 14-15 -0.21151326743569854 1106 16-17 -0.09637690970784973 1106 18-19 -0.02361612717114525 1106 20-21 0.11617854911514769 1106 22-23 0.3197059090398042 1106 24-25 -0.05584512539974895 1106 26-27 0.07735983858108142 1106 28-29 -0.0031073165828132687 1106 30-31 -0.1811008661559157 1106 32-33 -0.13156516901731408 1106 34-35 -0.4911875831998742 1106 36-37 -0.37933080342252623 1106 38-39 -0.08870412990891907 1106 40-41 -0.09339502905449848 1106 42-43 -0.6869277830689029 1106 44-45 -0.12010207284927077 1106 46-47 -0.1532976425442243 1106 48-49 -0.2367685234874628 1106 50-51 -0.30387169076001186 1106 52-53 0.09512528556734878 1106 54-55 0.11639171448332775 1106 56-57 0.14521559988064325 1106 58-59 0.09161345756168515 1106 60-61 0.16368454443018265 1106 62-63 -0.39619777690251823 1106 64-65 -0.21694718390673984 1106 66-67 -0.19060601242052044 1106 68-69 0.004324332975603085 1106 70-71 0.5787920233674946 1106 72-73 0.313449258050742 1106 74-75 -0.14417136816841492 1106 76-77 0.46207713090409896 1106 78-79 0.04497020807400176 1106 80-81 -0.19761457444703723 1106 82-83 -0.14000860396362924 1106 84-85 0.10656055929635855 1106 86-87 0.18131167857961827 1106 88-89 -0.27549830136276654 1106 90-91 -0.3547188695715029 1106 92-93 -0.012365262606245153 1106 94-95 0.3714031722339435 1106 96-97 0.4182635088094422 1106 98-99 0.2740451603124825 1106 100-101 0.3245730062621277 1107 1 0.07648719447262664 1107 2 -0.010710429355661688 1107 3 0.04654565835153335 1107 4 0.053126391579695564 1107 5 0.07523109577480369 1107 6 -0.14018940016783432 1107 7 -0.1649076220331338 1107 8 -0.00660088552984206 1107 9 -0.0010213978666726575 1107 10-11 -0.2493889700601244 1107 12-13 -0.09346479117379403 1107 14-15 -0.20325707697229944 1107 16-17 0.08685140183737161 1107 18-19 0.22326344933800613 1107 20-21 -7.987043747590405E-4 1107 22-23 -0.1366619045812243 1107 24-25 -0.21857739373600538 1107 26-27 0.005116492573471021 1107 28-29 0.10519724212414872 1107 30-31 0.1600027359346683 1107 32-33 0.40164769156123015 1107 34-35 0.26943663585452526 1107 36-37 0.2534467429639591 1107 38-39 0.26433030559337567 1107 40-41 0.07907134829842022 1107 42-43 0.051641860484011204 1107 44-45 -0.1662332851455517 1107 46-47 -0.045114004446176637 1107 48-49 -0.06642497460194363 1107 50-51 -0.3526631564521523 1107 52-53 -0.3602885988056741 1107 54-55 -0.18318909407248185 1107 56-57 -0.4489278119615179 1107 58-59 -0.279794192682175 1107 60-61 -0.1200825356058246 1107 62-63 -0.35861833878028193 1107 64-65 -0.64745814065747 1107 66-67 -0.2985209380543452 1107 68-69 -0.2208231229891453 1107 70-71 -0.22869564944820908 1107 72-73 -0.2404947691429591 1107 74-75 -0.1771625409639661 1107 76-77 -0.7606787841290235 1107 78-79 -0.6737483021840944 1107 80-81 -0.693832446513035 1107 82-83 -0.6464375496702743 1107 84-85 -0.5339962129295195 1107 86-87 -0.6986947705195199 1107 88-89 -1.0491886639902397 1107 90-91 -0.700679449453002 1107 92-93 -0.9807915480594964 1107 94-95 -0.900701786854583 1107 96-97 -1.099787250975048 1107 98-99 -1.3198228212538439 1107 100-101 -0.695049721438096 1108 1 -0.025947330574140892 1108 2 -0.007449672756202119 1108 3 -0.08179304462082371 1108 4 0.044993986674271014 1108 5 0.022123633708702073 1108 6 -0.2073661825415627 1108 7 -0.15845106414496257 1108 8 -0.15265939727376576 1108 9 -0.14552098215268217 1108 10-11 4.5001885951023723E-7 1108 12-13 0.353349800387214 1108 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-0.10906629121223688 2316 64-65 -0.027161641193018227 2316 66-67 -0.23714895234878242 2316 68-69 -0.5975886561776633 2316 70-71 -0.6721585469747104 2316 72-73 -0.5567992366551451 2316 74-75 -0.4664497210158203 2316 76-77 -0.0674686418853554 2316 78-79 -0.23417433247468722 2316 80-81 -0.43113712423753725 2316 82-83 -0.31546827069851346 2316 84-85 -0.1561846232239148 2316 86-87 -0.37162943453017405 2316 88-89 -0.5454548928814198 2316 90-91 -0.2225114998238773 2316 92-93 -0.2596481932711612 2316 94-95 -0.499066134846057 2316 96-97 0.056400498652074305 2316 98-99 0.086252314633402 2316 100-101 0.6468405877742107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 3.0 5 5.0 6 25.0 7 41.0 8 99.0 9 182.0 10 374.0 11 715.0 12 1161.0 13 1657.0 14 2123.0 15 3309.0 16 4688.0 17 6641.0 18 5729.0 19 3007.0 20 2154.0 21 2037.0 22 2064.0 23 2185.0 24 2493.0 25 2850.0 26 3111.0 27 3621.0 28 4254.0 29 5176.0 30 6268.0 31 7781.0 32 9724.0 33 12693.0 34 17298.0 35 25668.0 36 37180.0 37 66136.0 38 65370.0 39 6386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.36065573770492 23.169505013528568 17.091198472067486 19.37864077669903 2 14.470530948948317 23.36710045160736 41.71591701118039 20.446451588263937 3 17.02804803141838 28.45858356327436 34.05768735022007 20.455681055087187 4 10.541960657010735 20.821677153250523 41.44826247330615 27.18809971643259 5 10.027250385198203 40.61198762272224 36.57759356177815 12.783168430301409 6 24.866729681647044 42.09368863598028 18.947458873177577 14.092122809195097 7 23.237887916081867 38.01776513234737 22.809513352492434 15.934833599078328 8 24.14874081429358 36.23138591583363 21.584222067336917 18.035651202535878 9 25.54652765180086 17.062101581421402 23.652895665651425 33.73847510112631 10-11 21.95085468045358 29.66398375613839 29.406354328779067 18.97880723462896 12-13 23.460778291603145 29.178137561624567 29.71091753368067 17.65016661309162 14-15 20.918904812371306 29.512334068508107 30.306545601522544 19.262215517598047 16-17 16.805745183968735 32.836215154784526 31.22583232286584 19.132207338380898 18-19 16.53268026899122 29.782534666195648 36.835438606541466 16.849346458271672 20-21 17.25703428587796 28.91496478481021 37.19506955517152 16.632931374140306 22-23 17.350498074536137 28.683046370261927 37.11244072435632 16.854014830845614 24-25 17.00738835907775 29.481350877835084 37.217406802127876 16.293853960959293 26-27 16.768404268523618 29.274809281779206 37.75791118593793 16.19887526375924 28-29 16.28279266348195 29.389037233179188 37.97360992205825 16.35456018128061 30-31 17.018500307118465 29.239905668483917 37.437104366174324 16.304489658223297 32-33 16.516130880204702 29.585851545617437 37.41689501640617 16.481122557771688 34-35 17.074991009226284 29.477007488598428 37.01493582337983 16.433065678795458 36-37 17.456899981222808 29.064386670103847 36.57780917918214 16.900904169491202 38-39 16.215218436019107 30.304476927924227 36.735187501392375 16.745117134664287 40-41 16.713928353192788 29.871931968632243 36.90039718912313 16.51374248905184 42-43 16.862101014730786 29.507244439351695 36.518849547381684 17.111804998535835 44-45 17.37447129476689 29.581236812205812 35.80523915458084 17.23905273844646 46-47 17.42173253005146 29.950415485135785 35.43526483795921 17.192587146853548 48-49 17.118274026052557 30.263739970911267 35.05526541082266 17.562720592213513 50-51 17.147871971382287 30.290314470212692 34.566740184143775 17.995073374261246 52-53 17.595660241048215 31.149927914681534 33.90269595908482 17.35171588518543 54-55 17.916839056681837 30.08051939785494 33.85840679800134 18.144234747461887 56-57 18.16963760021132 30.54762564009535 33.445678222595646 17.837058537097683 58-59 18.003507197392835 30.48620194709924 33.37963979618791 18.13065105932001 60-61 17.985843907437996 30.58565740855667 33.67339144714857 17.755107236856762 62-63 17.69559308872064 30.28442670689441 33.790351069822506 18.229629134562444 64-65 17.58022475343002 30.21679699310336 34.01901906680543 18.18395918666119 66-67 17.60520796534813 30.48620194709924 33.21478242327608 18.693807664276555 68-69 17.943038276826716 29.766621792362457 33.153836116494965 19.13650381431586 70-71 17.892912724252174 30.214569190766717 33.70680848219827 18.185709602782843 72-73 17.852653153454206 29.38137111686096 34.096514762373054 18.669460967311775 74-75 17.628468813104593 30.456175080081664 33.05666106002187 18.85869504679188 76-77 17.381791216730157 29.950574613874114 33.61228601162913 19.0553481577666 78-79 17.39452151579671 30.045733599396584 32.92819156554035 19.631553319266352 80-81 17.323390969762357 29.670189776933338 32.97656670199325 20.029852551311063 82-83 17.097009296419238 30.179945068060228 33.12418246569936 19.59886316982117 84-85 17.13052693890411 29.514243613368084 32.90734570081887 20.447883746908925 86-87 16.699288058024703 29.3481132105496 32.90177619497726 21.05082253644844 88-89 17.115409708762584 29.031765278745812 33.14062843121342 20.712196581278185 90-91 16.539718815549495 28.752494660551104 33.687068507277885 21.02071801662152 92-93 16.696901704301332 28.385448990308877 33.9001607230948 21.017488582294998 94-95 16.264548344901993 28.312503381485687 33.98782983409237 21.43511843951994 96-97 16.971398200572228 28.37679139177177 32.96495030409502 21.686860103560985 98-99 16.46650509461195 28.792872158100856 32.42569727677357 22.31492547051363 100-101 15.842833791976163 27.74441630869183 33.56803962419406 22.84471027513795 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1110.0 1 714.5 2 390.0 3 495.5 4 422.0 5 407.5 6 753.0 7 1419.5 8 1982.0 9 2118.5 10 1769.0 11 1337.0 12 1223.5 13 1188.0 14 1132.0 15 1089.0 16 1049.0 17 1046.0 18 1097.0 19 1155.5 20 1303.0 21 1481.5 22 1702.5 23 1973.5 24 2278.0 25 2885.5 26 4087.5 27 5465.5 28 5865.0 29 6410.5 30 7819.5 31 8697.0 32 9747.5 33 11173.5 34 13396.5 35 15388.0 36 16803.5 37 17871.0 38 17023.5 39 15242.5 40 13736.5 41 12508.5 42 11229.5 43 10419.0 44 9986.0 45 9496.5 46 8563.0 47 7706.0 48 7693.0 49 6759.0 50 5237.5 51 4265.5 52 3449.5 53 2933.0 54 2498.5 55 2124.5 56 1790.0 57 1423.5 58 1124.5 59 907.0 60 695.0 61 518.0 62 370.5 63 246.5 64 160.5 65 109.5 66 73.0 67 57.5 68 50.0 69 36.0 70 24.0 71 16.0 72 11.0 73 8.5 74 3.5 75 2.5 76 4.0 77 4.5 78 4.0 79 2.5 80 2.0 81 1.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01941370607649 2 0.0 3 0.0 4 0.0 5 0.027688400469748034 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031825747666377052 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.1825747666377053E-4 24-25 1.5912873833188527E-4 26-27 0.0012730299066550821 28-29 6.365149533275411E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.547724299913115E-4 42-43 0.012412041589887051 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.1825747666377053E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.5912873833188527E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0012730299066550821 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012730299066550821 92-93 0.001909544859982623 94-95 0.0 96-97 0.0 98-99 0.0020686735983145082 100-101 0.018936319861494345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 314211.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.59404885125591 #Duplication Level Percentage of deduplicated Percentage of total 1 82.98604817408484 39.496420307700696 2 9.245057130566792 8.800194014096952 3 2.551499704684669 3.643086047661815 4 1.1405366332465412 2.171310249575313 5 0.6947284728926034 1.6532470438604494 6 0.4888880766476 1.396089780165745 7 0.3309683545692019 1.1026486824910466 8 0.2707967833493958 1.0310652268395284 9 0.2235874935469681 0.9577290681363839 >10 1.7399650700935305 16.93921171483156 >50 0.21614710087886094 7.111707857222468 >100 0.10100331816769205 8.221840614530326 >500 0.007406909998964083 2.2273160113422628 >1k 0.002693421817805121 3.5319783828781413 >5k 6.733554544512803E-4 1.716154998667312 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5355 1.7042687875344913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4534 1.4429793991935356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3490 1.110718593556559 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1805 0.5744547453781058 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1192 0.3793629121832145 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.287068243950721 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 744 0.23678356263784525 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 704 0.22405326357129446 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 683 0.21736985656135524 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 619 0.19700137805487394 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.18681713880163328 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 568 0.18077024674502165 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 559 0.17790592945504774 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 558 0.17758767197838396 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 514 0.16358434300517805 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 512 0.1629478280518505 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.1489444990786446 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 440 0.14003328973205903 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 436 0.13876025982540396 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 387 0.12316564346887919 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 368 0.11711875141226756 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 351 0.11170837430898345 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 350 0.11139011683231968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.5912873833188527E-4 0.0 22-23 0.0 0.0 0.0 7.956436916594263E-4 0.0 24-25 0.0 0.0 0.0 9.547724299913115E-4 0.0 26-27 0.0 0.0 0.0 0.0027051885516420496 0.0 28-29 0.0 0.0 0.0 0.0062060207949435255 0.0 30-31 0.0 0.0 0.0 0.014003328973205904 0.0 32-33 0.0 0.0 0.0 0.021004993459808854 0.0 34-35 0.0 0.0 0.0 0.02784752920807992 0.0 36-37 0.0 0.0 0.0 0.0345309362180191 0.0 38-39 0.0 0.0 0.0 0.04503343294792353 0.0 40-41 0.0 0.0 0.0 0.07431312080099042 0.0 42-43 0.0 0.0 0.0 0.12459780211386615 0.0 44-45 0.0 0.0 0.0 0.16931297758512592 0.0 46-47 0.0 0.0 0.0 0.2702005976875412 0.0 48-49 0.0 0.0 0.0 0.3666326131166636 0.0 50-51 0.0 0.0 0.0 0.4391953177960033 0.0 52-53 0.0 0.0 0.0 0.5257613514485489 0.0 54-55 0.0 0.0 0.0 0.5988014423428842 0.0 56-57 0.0 0.0 0.0 0.7806855902562291 0.0 58-59 0.0 0.0 0.0 1.001238021584222 0.0 60-61 0.0 0.0 0.0 1.209378411322328 0.0 62-63 0.0 0.0 0.0 1.4639843926533445 0.0 64-65 0.0 0.0 0.0 1.7952904258603295 0.0 66-67 0.0 0.0 0.0 2.228279722861389 0.0 68-69 0.0 0.0 0.0 2.7104398000070016 0.0 70-71 0.0 0.0 0.0 3.0835966913952726 0.0 72-73 0.0 0.0 0.0 3.400581138152388 0.0 74-75 0.0 0.0 0.0 3.8329339202001202 0.0 76-77 0.0 0.0 0.0 4.401819159736609 0.0 78-79 0.0 0.0 0.0 4.928217026138487 0.0 80-81 0.0 0.0 0.0 5.449045386698748 0.0 82-83 0.0 0.0 0.0 6.034957401236749 0.0 84-85 0.0 0.0 0.0 6.893616073275601 0.0 86-87 0.0 0.0 0.0 7.943547488789381 0.0 88-89 0.0 0.0 0.0 9.007800490753029 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 135 0.0 49.26512 9 GGTATCA 2120 0.0 44.823788 1 TAGGCAT 165 0.0 40.31424 5 CTGCTCG 50 0.001613086 38.00452 9 GGTACTC 75 6.900611E-6 38.00452 8 GTATGGT 85 1.6253478E-5 33.53874 1 GGCATTC 215 0.0 33.143475 7 GTATCAA 3230 0.0 32.95034 1 TGGTACT 90 2.4054692E-5 31.670435 7 GACGTCC 30 0.0039503793 31.665394 78-79 AAGCGTC 30 0.0039503793 31.665394 68-69 CGCTGTA 45 2.4118981E-5 31.665394 16-17 ATATCGG 30 0.0039503793 31.665394 50-51 CATGGGG 610 0.0 31.156204 4 TTAGGCA 220 0.0 30.23568 4 GTCTAGA 95 3.476251E-5 30.008343 1 AGGCATT 240 0.0 29.686306 6 GCATTCC 235 0.0 28.301237 8 CCTTTAG 220 1.8189894E-12 28.075989 1 ACGGAGG 85 6.392292E-4 27.9445 8 >>END_MODULE