FastQCFastQC Report
Thu 26 May 2016
SRR937646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328872
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45671.3886861757765938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33651.0231944343087889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.8057846213724488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20600.6263835169914131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.2642365418764748No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.20737551387773967No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6540.19886156316135153No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5650.17179936266997495No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5360.16298134228514435No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.13987204748351942No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA4510.13713542046753752No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4370.13287844510934343No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4300.13074995743024642No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4260.1295336787564767No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.12801333041426452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4180.12710112140893723No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4130.12558077306672505No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4080.12406042472451288No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.11828310102410665No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT3790.1152424043396823No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.11159356831837311No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.10216740859665767No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG250.005004985357.0376662
GGTATCA19400.038.9739531
GTATCAA30400.033.0056231
AGAGTAT650.00583226829.2367218
TATACTC1354.179783E-728.1710415
TGGGGGA2900.027.8504966
TATCAAC36400.027.5525342
ATCAACG36400.027.5525343
TCAACGC36550.027.4436444
CTAGCCG700.00834980927.1649344
GCTAAGG700.0083560227.1607952
CAGCGCA350.008399365527.13598488-89
GCGCAAT350.008399365527.13598490-91
CGCCGAC350.008399365527.13598492-93
AACAGCG350.008399365527.13598486-87
CAACGCA37250.026.9279235
AACGCAG37450.026.76786
CATGGGG8950.026.5578944
ACATGGG21700.026.5036753
TGGGTGC908.923271E-426.3942626
Produced by FastQC (version 0.11.5)