##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937646_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 328872 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96229840180982 33.0 31.0 34.0 30.0 34.0 2 32.076002213627184 33.0 31.0 34.0 30.0 34.0 3 32.087672407502005 34.0 31.0 34.0 30.0 34.0 4 35.66886813106619 37.0 35.0 37.0 35.0 37.0 5 35.68086063878956 37.0 35.0 37.0 35.0 37.0 6 35.80869456809944 37.0 35.0 37.0 35.0 37.0 7 35.77165888248315 37.0 35.0 37.0 35.0 37.0 8 35.76738366294485 37.0 35.0 37.0 35.0 37.0 9 37.40011311391666 39.0 37.0 39.0 35.0 39.0 10-11 37.32187294752974 39.0 37.0 39.0 35.0 39.0 12-13 37.23837085553042 39.0 37.0 39.0 34.0 39.0 14-15 38.37645345301516 40.0 38.0 41.0 34.0 41.0 16-17 38.36032255710428 40.0 38.0 41.0 34.0 41.0 18-19 38.42420607409569 40.0 38.0 41.0 34.0 41.0 20-21 38.29267921866258 40.0 38.0 41.0 34.0 41.0 22-23 38.20903269357075 40.0 38.0 41.0 34.0 41.0 24-25 38.09657860809069 40.0 38.0 41.0 34.0 41.0 26-27 38.12260545136102 40.0 38.0 41.0 34.0 41.0 28-29 38.04343331144032 40.0 38.0 41.0 34.0 41.0 30-31 37.77526058770586 40.0 37.0 41.0 33.5 41.0 32-33 37.45677041523754 40.0 36.0 41.0 33.0 41.0 34-35 37.19914586830134 39.0 35.0 41.0 33.0 41.0 36-37 37.109250711523025 39.0 35.0 41.0 33.0 41.0 38-39 37.013450521783554 40.0 35.0 41.0 33.0 41.0 40-41 36.819562018049574 40.0 35.0 41.0 31.5 41.0 42-43 36.50647364324114 39.0 35.0 41.0 31.0 41.0 44-45 36.150855043907654 39.0 35.0 41.0 30.0 41.0 46-47 35.64618757449707 39.0 35.0 41.0 28.0 41.0 48-49 35.198005607044685 39.0 35.0 41.0 25.0 41.0 50-51 34.27094583911066 38.0 34.0 40.0 23.5 40.5 52-53 34.37279701525213 38.5 35.0 40.0 23.0 40.5 54-55 34.69225564961444 39.0 35.0 40.0 23.0 41.0 56-57 34.48454109805638 39.0 35.0 40.5 20.0 41.0 58-59 34.292694422146 39.0 35.0 40.0 18.5 41.0 60-61 34.02966807754993 38.5 34.5 40.0 14.0 41.0 62-63 33.69994557152935 38.0 34.0 40.0 8.0 41.0 64-65 33.38208026222968 38.0 34.0 40.0 4.5 41.0 66-67 32.979438809019925 37.5 33.5 40.0 2.0 41.0 68-69 32.509055194726216 37.0 33.0 39.5 2.0 41.0 70-71 32.10550153251113 36.0 32.5 39.0 2.0 40.5 72-73 31.65044150915858 36.0 32.0 39.0 2.0 40.0 74-75 31.150842881120923 35.0 31.0 38.0 2.0 40.0 76-77 30.69182995207862 35.0 31.0 37.0 2.0 39.5 78-79 30.149109684010803 35.0 30.5 37.0 2.0 39.0 80-81 29.59797884891386 35.0 30.0 36.0 2.0 39.0 82-83 29.081458743827383 34.0 29.0 36.0 2.0 37.5 84-85 28.609566943978205 34.0 29.0 35.5 2.0 37.0 86-87 28.124901177357756 34.0 28.5 35.0 2.0 36.5 88-89 27.626313580967672 34.0 27.0 35.0 2.0 36.0 90-91 27.149863776788536 34.0 26.0 35.0 2.0 36.0 92-93 26.673905045123938 33.5 25.5 35.0 2.0 35.0 94-95 26.10118678391593 33.0 24.5 35.0 2.0 35.0 96-97 25.437963706244375 33.0 20.0 35.0 2.0 35.0 98-99 24.685306137342188 32.0 9.0 35.0 2.0 35.0 100-101 22.3607193680216 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15683140621845482 1101 2 0.35976038996987114 1101 3 0.255798907857276 1101 4 0.06971999894505387 1101 5 0.11112049978878247 1101 6 0.19980606893575015 1101 7 0.19268131319429926 1101 8 0.3948260602425222 1101 9 0.5197111074217631 1101 10-11 0.26147105944235705 1101 12-13 0.33529143643870896 1101 14-15 0.2623824902867824 1101 16-17 0.5125648796107498 1101 18-19 0.40204703534634945 1101 20-21 0.6501731529781622 1101 22-23 0.333815721932865 1101 24-25 0.2813575759703113 1101 26-27 0.39129989276679567 1101 28-29 0.15217473691844674 1101 30-31 0.1735896938588155 1101 32-33 0.2733492875217962 1101 34-35 0.3345099908864455 1101 36-37 0.4705230422505977 1101 38-39 0.20731174991474433 1101 40-41 0.49248454985232826 1101 42-43 0.6633088543624979 1101 44-45 0.7850215273528178 1101 46-47 0.7418384710334109 1101 48-49 0.8429924110703482 1101 50-51 0.9517762241093806 1101 52-53 0.8282477140663431 1101 54-55 1.1960921217305582 1101 56-57 1.420670286804068 1101 58-59 1.6865017489201435 1101 60-61 1.605295092019162 1101 62-63 1.3687563541070915 1101 64-65 1.296945770957251 1101 66-67 1.5577453925797116 1101 68-69 1.4574131581636252 1101 70-71 1.5055956785068396 1101 72-73 1.328614504188515 1101 74-75 1.534817267398239 1101 76-77 1.504087030115187 1101 78-79 1.597051843425561 1101 80-81 1.5471606558319202 1101 82-83 1.7221064498201528 1101 84-85 1.7212040491201108 1101 86-87 1.6046908653848568 1101 88-89 1.406916567070997 1101 90-91 1.9035538645206067 1101 92-93 1.753357934792838 1101 94-95 1.5734445870623937 1101 96-97 1.7456868463104556 1101 98-99 2.064408042919954 1101 100-101 2.377026349441259 1102 1 0.45746695802331416 1102 2 0.3729958874214674 1102 3 0.5098575235653655 1102 4 0.30221848515887473 1102 5 0.3995217985458055 1102 6 0.3633998688489726 1102 7 0.37336301759834356 1102 8 0.312023148051658 1102 9 0.4136697894026966 1102 10-11 0.34450759532743547 1102 12-13 0.33554651537014735 1102 14-15 0.45662867244651295 1102 16-17 0.668747087020904 1102 18-19 0.589928163796479 1102 20-21 0.6234208560758177 1102 22-23 0.5360885467293954 1102 24-25 0.562280532597363 1102 26-27 0.7276345753961593 1102 28-29 0.5684111068830262 1102 30-31 0.722605375253309 1102 32-33 0.5228474733622903 1102 34-35 0.7638292877268071 1102 36-37 0.8883447158544513 1102 38-39 0.942706693153724 1102 40-41 0.9626379309593247 1102 42-43 1.3157697700720234 1102 44-45 1.214829526818825 1102 46-47 1.3079706639016848 1102 48-49 1.177911048042965 1102 50-51 1.2596755656719907 1102 52-53 1.4302220726739492 1102 54-55 1.5885465531906107 1102 56-57 1.8410522853832632 1102 58-59 1.8298990188604023 1102 60-61 1.6573764884213489 1102 62-63 1.813280262393107 1102 64-65 1.8662986333242841 1102 66-67 1.9211470489240199 1102 68-69 1.9925067745153768 1102 70-71 1.930681141868444 1102 72-73 1.7267951000719677 1102 74-75 1.7785463783408915 1102 76-77 1.9408298390348406 1102 78-79 2.0789936854291966 1102 80-81 2.3843750031874436 1102 82-83 2.430780087055002 1102 84-85 2.4780542295824937 1102 86-87 2.4895191519696134 1102 88-89 2.3264498918222323 1102 90-91 2.473166573649575 1102 92-93 2.450281253775003 1102 94-95 2.0702429888856138 1102 96-97 2.211699366633333 1102 98-99 2.639422703998722 1102 100-101 3.0116198203741824 1103 1 0.20242447110203088 1103 2 0.3095074239057851 1103 3 0.21635000897466483 1103 4 0.08639702094767898 1103 5 0.27576541038771296 1103 6 0.161609672592661 1103 7 0.13930695300612683 1103 8 0.27091213773988443 1103 9 0.26721843012362 1103 10-11 0.42023561855160096 1103 12-13 0.2634623882420186 1103 14-15 0.4838066941586945 1103 16-17 0.32107252776602024 1103 18-19 0.4038173046837059 1103 20-21 0.39492891498295535 1103 22-23 0.3585279915266355 1103 24-25 0.37815038049115657 1103 26-27 0.13821548198541223 1103 28-29 0.32315119578803575 1103 30-31 0.2643665413000562 1103 32-33 0.4761042999975089 1103 34-35 0.6420536065752742 1103 36-37 0.7785893558496113 1103 38-39 0.9249710494995469 1103 40-41 1.2554952898150518 1103 42-43 1.1077426147301708 1103 44-45 1.1480524848762172 1103 46-47 1.4111186272510565 1103 48-49 1.4869315808682657 1103 50-51 1.3922922157067674 1103 52-53 1.3487636070414837 1103 54-55 1.354664367457751 1103 56-57 1.6063611849568744 1103 58-59 1.5036191192565482 1103 60-61 1.3877886569175217 1103 62-63 1.5717776466502613 1103 64-65 1.9169049413469068 1103 66-67 2.1132302688581035 1103 68-69 2.4083116068356745 1103 70-71 2.130959348043408 1103 72-73 2.1572435563301013 1103 74-75 2.2831579480889914 1103 76-77 1.975802169616351 1103 78-79 1.660520190615319 1103 80-81 1.783222866745458 1103 82-83 1.6760399981499852 1103 84-85 1.9030141618453378 1103 86-87 1.7304169716799152 1103 88-89 1.6509154033284759 1103 90-91 1.6971301044488136 1103 92-93 2.027937064352887 1103 94-95 1.7883399981692314 1103 96-97 1.833036570661271 1103 98-99 1.837705083266318 1103 100-101 2.254143029421929 1104 1 0.3107681821854662 1104 2 0.26701053723081714 1104 3 0.22251179809877897 1104 4 0.300606755277272 1104 5 0.20733712064092913 1104 6 0.25150693286663284 1104 7 0.19075464914560314 1104 8 0.3764798006705661 1104 9 0.5182691773216277 1104 10-11 0.40588189293931265 1104 12-13 0.2566909062993048 1104 14-15 0.5621781657236653 1104 16-17 0.6576813384758537 1104 18-19 0.48307737110130944 1104 20-21 0.7387567481087345 1104 22-23 0.481368122286284 1104 24-25 0.3487294589469485 1104 26-27 0.5544864969293783 1104 28-29 0.5279171775232001 1104 30-31 0.7989179630518279 1104 32-33 0.744661014401828 1104 34-35 0.6690162985636476 1104 36-37 0.8071216825411867 1104 38-39 0.909261730279951 1104 40-41 1.2564682038872732 1104 42-43 1.2718861909028547 1104 44-45 1.4311964242701478 1104 46-47 1.3716183159185462 1104 48-49 1.3379395198470903 1104 50-51 1.5277996876868443 1104 52-53 1.4778433600510326 1104 54-55 1.2806904674992623 1104 56-57 1.4361725693487344 1104 58-59 1.4644690569900476 1104 60-61 1.5227123804542657 1104 62-63 1.2718801269325368 1104 64-65 1.5086701966395637 1104 66-67 1.720082178364116 1104 68-69 1.6480700647020186 1104 70-71 1.5270650973169673 1104 72-73 1.159215328849811 1104 74-75 1.092752178059932 1104 76-77 0.9363991496910735 1104 78-79 1.0443477642973455 1104 80-81 1.0804416297176473 1104 82-83 1.1919179558087656 1104 84-85 1.388731783265012 1104 86-87 1.3520831194590777 1104 88-89 1.2486478649308026 1104 90-91 1.4424276864330388 1104 92-93 1.5486406758099243 1104 94-95 1.5008633481069644 1104 96-97 1.7614560393204677 1104 98-99 1.759709805142613 1104 100-101 1.2651693889071929 1105 1 0.1200598369029855 1105 2 0.02012926392998793 1105 3 -0.2486399799339125 1105 4 -0.13880212054721852 1105 5 0.015311382183803346 1105 6 0.05336024516947191 1105 7 0.10685373145130939 1105 8 -0.0759685790254494 1105 9 -0.20311560902766956 1105 10-11 0.007486961766382194 1105 12-13 -0.0701122195968864 1105 14-15 -0.023089435366102862 1105 16-17 -0.06485504666896702 1105 18-19 -0.18657060710344808 1105 20-21 -0.01144937463288187 1105 22-23 -0.13813377762767232 1105 24-25 -0.30265425197934803 1105 26-27 -0.03630160889225209 1105 28-29 -0.20049370575755887 1105 30-31 -0.05644212441591634 1105 32-33 -0.06188383073725845 1105 34-35 0.14817549701503907 1105 36-37 -0.14244545970142752 1105 38-39 -0.35560599269108195 1105 40-41 -0.052939954988545423 1105 42-43 -0.257997125701678 1105 44-45 -0.22392438158014727 1105 46-47 0.1922646451413783 1105 48-49 0.22622547482271926 1105 50-51 0.1006237484840895 1105 52-53 0.05644333854257155 1105 54-55 0.06049312737881252 1105 56-57 0.2541796312931055 1105 58-59 0.5230975341910735 1105 60-61 0.7433264469870977 1105 62-63 0.41983592704724515 1105 64-65 0.6912232508408849 1105 66-67 0.38918348321069374 1105 68-69 0.5094704446848084 1105 70-71 0.6965428002134395 1105 72-73 0.7913489680812518 1105 74-75 0.9814279738729539 1105 76-77 0.8309942529316814 1105 78-79 1.0956857697461544 1105 80-81 1.1193002474406164 1105 82-83 0.856887843964774 1105 84-85 1.2257962369501278 1105 86-87 1.2071429846727284 1105 88-89 1.473823517354088 1105 90-91 0.9281738507576698 1105 92-93 1.0243719634497275 1105 94-95 1.0044391295810122 1105 96-97 0.6239632088071332 1105 98-99 0.8386413723923987 1105 100-101 1.366850633530074 1106 1 0.10470757612486281 1106 2 0.24167383689411537 1106 3 0.17286954220652717 1106 4 0.20420524012575925 1106 5 0.15765277720658588 1106 6 0.17419211805182044 1106 7 0.16705936295031876 1106 8 -0.02086868417791976 1106 9 0.12338281368526083 1106 10-11 -0.08526920133677862 1106 12-13 -0.3429303058219091 1106 14-15 -0.4165991911786975 1106 16-17 -0.1976743012547857 1106 18-19 -0.08674586122192096 1106 20-21 0.26945961006159536 1106 22-23 -0.08099509320192766 1106 24-25 -0.2592671740496897 1106 26-27 0.09052395484183506 1106 28-29 0.15407458493060489 1106 30-31 0.1809571044659677 1106 32-33 0.05626665413247167 1106 34-35 0.1307902884626344 1106 36-37 0.4006086354031382 1106 38-39 0.2568837841520093 1106 40-41 0.22046231163138685 1106 42-43 0.2327552986469641 1106 44-45 0.5424969293206559 1106 46-47 0.9275316155818487 1106 48-49 1.2396966747292524 1106 50-51 1.1280101253972745 1106 52-53 1.1180685284012029 1106 54-55 1.3901285988123888 1106 56-57 1.603291677092848 1106 58-59 1.7257906058115893 1106 60-61 1.3682300572219432 1106 62-63 1.5670084939359015 1106 64-65 1.3608166612860302 1106 66-67 1.8104651749971126 1106 68-69 1.7084130781700253 1106 70-71 1.6529278919297674 1106 72-73 1.80399647363096 1106 74-75 1.6642589120666713 1106 76-77 1.941860008276933 1106 78-79 2.005827141401724 1106 80-81 1.8269062761822923 1106 82-83 1.1580795719703794 1106 84-85 0.9729279112111406 1106 86-87 1.249747260873221 1106 88-89 1.4483532521361902 1106 90-91 1.26491295579331 1106 92-93 1.4456945478637948 1106 94-95 1.2295880955817111 1106 96-97 1.2482826386470691 1106 98-99 1.9051327849405943 1106 100-101 1.7447569309947362 1107 1 0.1713742427915257 1107 2 -0.06837907315879121 1107 3 0.18027694961393337 1107 4 0.3089671448876601 1107 5 0.22749404704785547 1107 6 0.3413878852475847 1107 7 0.12208581797676743 1107 8 0.1838932205839825 1107 9 0.24719233749478775 1107 10-11 0.37848741241983674 1107 12-13 0.4737363608447609 1107 14-15 0.30112567654617095 1107 16-17 0.1517965982161087 1107 18-19 0.41484144036537884 1107 20-21 0.28109982170180814 1107 22-23 -0.026762288969123915 1107 24-25 0.35554764076513123 1107 26-27 0.3815292458471191 1107 28-29 0.5680957489517695 1107 30-31 0.5888936124024795 1107 32-33 0.4615576594234696 1107 34-35 0.900631558303914 1107 36-37 1.0170107518052518 1107 38-39 0.9748732021414241 1107 40-41 1.0373935285626033 1107 42-43 1.1306388965305558 1107 44-45 0.5551953420190685 1107 46-47 0.5198596579098904 1107 48-49 0.22117815621073333 1107 50-51 0.2586979560851077 1107 52-53 0.36357117390384275 1107 54-55 0.3798111384949294 1107 56-57 0.3511752749764483 1107 58-59 -0.009394579373591228 1107 60-61 -0.3587540697621847 1107 62-63 -0.503890976963568 1107 64-65 -0.5923579418885723 1107 66-67 0.014697979229921998 1107 68-69 0.3319580517149987 1107 70-71 0.023033712035587683 1107 72-73 -0.11372839409390423 1107 74-75 0.21531711312486834 1107 76-77 0.5958282622451847 1107 78-79 0.4888959244705049 1107 80-81 0.3784935777695928 1107 82-83 0.4191906830814922 1107 84-85 0.2073194456026748 1107 86-87 0.21720757833353588 1107 88-89 0.7091998129827495 1107 90-91 0.8143838552642073 1107 92-93 0.6541601563294037 1107 94-95 0.39942936542298213 1107 96-97 0.8377006280650576 1107 98-99 0.954339134146938 1107 100-101 1.3251802114180151 1108 1 0.07109876069786125 1108 2 -0.0604688016121564 1108 3 0.05851386421448268 1108 4 0.17638154866570233 1108 5 0.30108712064092913 1108 6 0.06090775931291148 1108 7 0.1871389466662663 1108 8 0.2581946767036243 1108 9 0.3837133921976559 1108 10-11 0.31148623178228974 1108 12-13 0.07538925340674041 1108 14-15 0.2931957277071291 1108 16-17 0.16891584260808656 1108 18-19 0.16334596614263575 1108 20-21 0.30668030182774686 1108 22-23 0.13490634542678492 1108 24-25 0.3270352440709132 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-1.0152246729879622 2316 96-97 -1.0362272856418642 2316 98-99 -0.5660765459372463 2316 100-101 -0.7730965578532292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1085.0 3 146.0 4 132.0 5 143.0 6 170.0 7 238.0 8 246.0 9 328.0 10 409.0 11 718.0 12 1207.0 13 1758.0 14 2137.0 15 2916.0 16 4149.0 17 5819.0 18 4693.0 19 2680.0 20 2403.0 21 2382.0 22 2443.0 23 2629.0 24 2923.0 25 3220.0 26 3524.0 27 4075.0 28 4643.0 29 5502.0 30 6793.0 31 8131.0 32 10360.0 33 13615.0 34 18878.0 35 28277.0 36 40036.0 37 65052.0 38 65358.0 39 9654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.02659498229746 23.39754634528649 15.760904369676659 19.814954302739395 2 14.055831931133708 23.690162655156435 41.50815714390226 20.745848269807592 3 17.235073045985594 29.46711330433643 32.5209323307354 20.77688131894258 4 10.5185347757568 20.591113183908995 41.30628941745235 27.58406262288186 5 10.145523934937938 41.57125612700271 35.678359792230594 12.604860145828761 6 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16.565102100185662 28-29 16.53919563008672 31.97068039220764 34.68100760256455 16.80911637514109 30-31 17.42822587524219 31.631033474281956 34.34236501224669 16.598375638229165 32-33 16.81465402338246 32.03198903233177 34.191705700902546 16.961651243383223 34-35 17.311673178422097 32.06753947037723 33.840564809102744 16.780222542097928 36-37 17.58983848491079 31.576694918480953 33.59315864421751 17.240307952390744 38-39 16.511711843421768 32.72996133363182 33.62374989149355 17.134576931452862 40-41 16.90090869357975 32.372760817291216 33.88831612242008 16.83801436670895 42-43 17.003784677007896 32.113860142096726 33.42958749305127 17.452767687844105 44-45 17.40693865055903 32.25669869612829 32.9554679301779 17.380894723134773 46-47 17.505993012467293 32.439178919216936 32.55294716097216 17.50188090734361 48-49 17.35554059364918 32.40850472670995 32.590078631249604 17.645876048391273 50-51 17.22474482451878 32.40580098673438 32.32141524335538 18.048038945391458 52-53 17.436438063579455 33.11634397561269 31.984881331247227 17.462336629560625 54-55 17.805917841101383 32.45597868882671 31.706767272300805 18.0313361977711 56-57 17.904704182155143 32.94800592686444 31.25035215503654 17.896937735943872 58-59 17.93412959356007 32.405699814057044 31.606343076175698 18.053827516207193 60-61 17.991189641966905 32.57426601921347 31.70465048642739 17.72989385239224 62-63 17.66546670565017 32.277580829861364 32.11646931267207 17.940483151816387 64-65 17.616137682560808 32.13359545240666 32.116540454607055 18.133726410425478 66-67 17.753795879086155 32.28465400434598 31.433122328494484 18.52842778807338 68-69 17.556541654738048 31.81362725450902 31.70535600075531 18.924475089997625 70-71 17.712002363407386 32.10183362038378 32.071986465228825 18.114177550980006 72-73 17.89570824012414 31.424733925506754 32.13223548258885 18.547322351780252 74-75 17.45759536758804 32.402972322191445 31.594858899984924 18.544573410235596 76-77 17.428501812201137 32.02646727377943 31.888039472481957 18.65699144153748 78-79 17.546061228157278 31.89425944144764 31.28249566724437 19.277183663150712 80-81 17.56017555454709 31.67139258857677 31.306364613758674 19.462067243117463 82-83 17.71708033064697 31.927926391614758 31.34979665729255 19.005196620445723 84-85 17.722632982934822 31.43019842381573 31.226171480078445 19.620997113171004 86-87 17.581944061672548 31.396752842366837 31.322905357977582 19.698397737983033 88-89 18.18617881474398 30.826310868552248 31.465718088265298 19.52179222843847 90-91 17.7720553635958 30.638807206587103 32.00051166707529 19.588625762741803 92-93 18.21927820922796 30.550784224151055 31.883813004416016 19.346124562204963 94-95 18.028460700934225 30.575685820662564 31.738326011314232 19.657527467088983 96-97 18.579669359063384 30.827485593850128 30.783018408196583 19.809826638889906 98-99 18.37575078276337 30.85184147366626 30.667572276166226 20.10483546740415 100-101 18.836517012189084 30.178230184168655 31.32374549856487 19.661507305077393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1089.0 1 704.0 2 379.0 3 439.5 4 392.0 5 402.5 6 727.0 7 1439.0 8 2093.5 9 2161.5 10 1730.5 11 1343.5 12 1252.0 13 1263.5 14 1210.0 15 1092.0 16 1017.0 17 1075.5 18 1137.5 19 1206.0 20 1369.5 21 1544.0 22 1717.0 23 1984.0 24 2314.0 25 3018.0 26 4373.5 27 5675.5 28 6026.5 29 6529.5 30 7798.0 31 9183.0 32 10708.0 33 12353.5 34 14637.5 35 16250.0 36 17658.5 37 19069.0 38 18037.0 39 16051.5 40 14451.0 41 13011.5 42 11690.5 43 10808.5 44 10452.5 45 9902.5 46 8862.5 47 7876.5 48 7757.0 49 6945.0 50 5451.5 51 4394.5 52 3545.5 53 2950.0 54 2538.0 55 2182.0 56 1859.0 57 1535.0 58 1155.0 59 905.0 60 735.5 61 577.0 62 422.5 63 276.5 64 182.5 65 131.0 66 91.5 67 79.0 68 62.0 69 33.0 70 22.5 71 15.0 72 11.5 73 10.0 74 10.0 75 7.5 76 3.5 77 4.5 78 4.0 79 3.0 80 2.0 81 1.0 82 1.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28977839402563915 2 0.24812084944902577 3 0.24051910773796492 4 0.24720864044369845 5 0.24812084944902577 6 0.1939964484662726 7 0.18304994040234498 8 0.18426621907611473 9 0.1939964484662726 10-11 0.19369237879783016 12-13 0.19034761244496337 14-15 0.19247610012406044 16-17 0.18016127855214187 18-19 0.18274587073390255 20-21 0.17331971101218713 22-23 0.18031331338636308 24-25 0.1832019752365662 26-27 0.17985720888369944 28-29 0.19080371694762704 30-31 0.1885231944343088 32-33 0.19293220462672406 34-35 0.18837115960008757 36-37 0.17468802452017806 38-39 0.16739035247755965 40-41 0.16526186479846264 42-43 0.17468802452017806 44-45 0.17651244253083265 46-47 0.1742319200175144 48-49 0.19186796078717555 50-51 0.18776302026320268 52-53 0.20327057335376683 54-55 0.1804653482205843 56-57 0.16313337711936557 58-59 0.16587000413534747 60-61 0.15461942640297743 62-63 0.16054878493760488 64-65 0.15887640176117152 66-67 0.15887640176117152 68-69 0.16115692427448977 70-71 0.16191709844559585 72-73 0.1622211681140383 74-75 0.17529616385706293 76-77 0.164805760295799 78-79 0.16997494465932034 80-81 0.16450169062735653 82-83 0.14762582402880148 84-85 0.14716971952613783 86-87 0.14975431170789852 88-89 0.15842029725850787 90-91 0.1622211681140383 92-93 0.1587243669269503 94-95 0.1594845410980564 96-97 0.1641976209589141 98-99 0.16663017830645357 100-101 0.1534031477292077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 328872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.64272453706434 #Duplication Level Percentage of deduplicated Percentage of total 1 83.87116611882404 41.63593196239145 2 8.813434927350979 8.750458446476525 3 2.428728312935309 3.6170607184324965 4 1.0862893972273546 2.157054612563649 5 0.5837950085149975 1.4490587396911574 6 0.44663595211389456 1.3303335323483714 7 0.3134697200582784 1.0893043674494651 8 0.2445622843918267 0.9712590492974913 9 0.21421960904156206 0.9571000537879093 >10 1.6863949694948324 17.164316633438435 >50 0.20056325338991005 6.80585237076943 >100 0.10520342432415529 9.201522178538028 >500 0.0030761235182501546 1.0096996920554542 >1k 0.0024608988146001235 3.861047642760148 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4567 1.3886861757765938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3365 1.0231944343087889 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2650 0.8057846213724488 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2060 0.6263835169914131 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 869 0.2642365418764748 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 682 0.20737551387773967 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 654 0.19886156316135153 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 565 0.17179936266997495 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 536 0.16298134228514435 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 460 0.13987204748351942 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 451 0.13713542046753752 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 437 0.13287844510934343 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 430 0.13074995743024642 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 426 0.1295336787564767 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 421 0.12801333041426452 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 418 0.12710112140893723 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 413 0.12558077306672505 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 408 0.12406042472451288 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 389 0.11828310102410665 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 379 0.1152424043396823 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.11159356831837311 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 336 0.10216740859665767 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.081393368848671E-4 0.0 20-21 0.0 0.0 0.0 6.081393368848671E-4 0.0 22-23 0.0 0.0 0.0 9.122090053273006E-4 0.0 24-25 0.0 0.0 0.0 0.0024325573475394682 0.0 26-27 0.0 0.0 0.0 0.0031927315186455517 0.0 28-29 0.0 0.0 0.0 0.007145637208397188 0.0 30-31 0.0 0.0 0.0 0.013835169914130724 0.0 32-33 0.0 0.0 0.0 0.022045050962076432 0.0 34-35 0.0 0.0 0.0 0.028582548833588753 0.0 36-37 0.0 0.0 0.0 0.03785667372108298 0.0 38-39 0.0 0.0 0.0 0.05184387846943492 0.0 40-41 0.0 0.0 0.0 0.07997032280036001 0.0 42-43 0.0 0.0 0.0 0.12801333041426452 0.0 44-45 0.0 0.0 0.0 0.176056338028169 0.0 46-47 0.0 0.0 0.0 0.28415310515945413 0.0 48-49 0.0 0.0 0.0 0.38844900143520883 0.0 50-51 0.0 0.0 0.0 0.4641623488773748 0.0 52-53 0.0 0.0 0.0 0.5531027268967866 0.0 54-55 0.0 0.0 0.0 0.6245590989807586 0.0 56-57 0.0 0.0 0.0 0.8316305431900557 0.0 58-59 0.0 0.0 0.0 1.0735179644360116 0.0 60-61 0.0 0.0 0.0 1.3040027731153763 0.0 62-63 0.0 0.0 0.0 1.5607896081150114 0.0 64-65 0.0 0.0 0.0 1.895114208567467 0.0 66-67 0.0 0.0 0.0 2.3224841275633077 0.0 68-69 0.0 0.0 0.0 2.8071711790605463 0.0 70-71 0.0 0.0 0.0 3.1931876231482157 0.0 72-73 0.0 0.0 0.0 3.4989296747670826 0.0 74-75 0.0 0.0 0.0 3.89847721910044 0.0 76-77 0.0 0.0 0.0 4.42816658152716 0.0 78-79 0.0 0.0 0.0 4.9329222311416 0.0 80-81 0.0 0.0 0.0 5.423994745676129 0.0 82-83 0.0 0.0 0.0 6.0021832202194165 0.0 84-85 0.0 0.0 0.0 6.864980904424822 0.0 86-87 0.0 0.0 0.0 7.898361672626432 0.0 88-89 0.0 0.0 0.0 8.969143010046462 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGGG 25 0.0050049853 57.037666 2 GGTATCA 1940 0.0 38.973953 1 GTATCAA 3040 0.0 33.005623 1 AGAGTAT 65 0.005832268 29.236721 8 TATACTC 135 4.179783E-7 28.171041 5 TGGGGGA 290 0.0 27.850496 6 TATCAAC 3640 0.0 27.552534 2 ATCAACG 3640 0.0 27.552534 3 TCAACGC 3655 0.0 27.443644 4 CTAGCCG 70 0.008349809 27.164934 4 GCTAAGG 70 0.00835602 27.160795 2 CAGCGCA 35 0.0083993655 27.135984 88-89 GCGCAAT 35 0.0083993655 27.135984 90-91 CGCCGAC 35 0.0083993655 27.135984 92-93 AACAGCG 35 0.0083993655 27.135984 86-87 CAACGCA 3725 0.0 26.927923 5 AACGCAG 3745 0.0 26.7678 6 CATGGGG 895 0.0 26.557894 4 ACATGGG 2170 0.0 26.503675 3 TGGGTGC 90 8.923271E-4 26.394262 6 >>END_MODULE