FastQCFastQC Report
Thu 26 May 2016
SRR937646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328872
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55561.6894110778661608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46401.4108832615728917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37341.1353961419640468No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17380.5284730837529495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.3618429054464959No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.26818944756622637No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.22197085796297647No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7180.21832202194166728No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA7010.2131528375781459No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6780.20615923520396992No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.19095575178184826No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5890.17909703471259336No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5760.1751441290228417No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.1742319200175144No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC5720.17392785034907196No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.15963657593227762No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT4980.1514266948843319No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT4890.14869006786835No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4870.14808192853146512No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT3790.1152424043396823No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA3530.10733659296017904No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT3390.10307961760198496No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC3350.10186333892821524No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGC250.00502051456.9930657
GGTATCA17850.044.1817321
CATTCCG1550.042.8980069
GTATCAA30450.037.2892341
TTAGGCA2300.037.169394
CCTTTAG2050.037.0799371
GGCATTC2000.033.2459567
TATGAGA1351.19307515E-831.6628154
GTATAGG1701.07320375E-1030.7409041
GCATTCC2200.030.2235958
TAGGCAT2200.030.2235955
CTACACG650.005841557429.2272154
CTTTAGG2350.028.294432
CTGGTCG856.4019323E-427.9377789
TATCAAC41200.027.3207072
TCAACGC41300.027.2545554
ATCAACG41450.027.1559263
TTGATCG700.00838798927.1395579
TACACGG700.00838798927.1395575
CAACGCA42200.026.6732965
Produced by FastQC (version 0.11.5)