##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937646_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 328872 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1966661801552 33.0 31.0 34.0 31.0 34.0 2 32.392699895400035 34.0 31.0 34.0 31.0 34.0 3 32.4481105110803 34.0 31.0 34.0 31.0 34.0 4 36.1119006786835 37.0 35.0 37.0 35.0 37.0 5 36.04641015349437 37.0 35.0 37.0 35.0 37.0 6 36.09710768931377 37.0 35.0 37.0 35.0 37.0 7 36.05372911041378 37.0 35.0 37.0 35.0 37.0 8 36.03915809190202 37.0 35.0 37.0 35.0 37.0 9 37.692682867498604 39.0 37.0 39.0 35.0 39.0 10-11 37.645159819017735 39.0 37.0 39.0 35.0 39.0 12-13 37.56135973874334 39.0 37.0 39.0 35.0 39.0 14-15 38.69323931499185 40.0 38.0 41.0 34.5 41.0 16-17 38.68387700990051 40.0 38.0 41.0 34.5 41.0 18-19 38.797887932083 40.0 38.0 41.0 35.0 41.0 20-21 38.78021692330147 40.0 38.0 41.0 35.0 41.0 22-23 38.62668150526649 40.0 38.0 41.0 34.0 41.0 24-25 38.64562352526211 40.0 38.0 41.0 34.0 41.0 26-27 38.473386302269574 40.0 38.0 41.0 34.0 41.0 28-29 38.32254798219368 40.0 38.0 41.0 34.0 41.0 30-31 38.159207837699775 40.0 37.0 41.0 34.0 41.0 32-33 37.905498187744776 40.0 36.5 41.0 33.5 41.0 34-35 37.70192658541925 40.0 36.0 41.0 33.0 41.0 36-37 37.46611143545209 40.0 35.5 41.0 33.0 41.0 38-39 37.256539018219854 40.0 35.0 41.0 33.0 41.0 40-41 37.01702029969107 40.0 35.0 41.0 33.0 41.0 42-43 36.68594316329757 40.0 35.0 41.0 31.5 41.0 44-45 36.1722433043859 39.0 35.0 41.0 30.5 41.0 46-47 35.55533003721813 39.0 35.0 41.0 28.0 41.0 48-49 35.04139148361672 39.0 35.0 41.0 24.0 41.0 50-51 34.734492446909435 39.0 35.0 41.0 22.5 41.0 52-53 34.509804726458924 39.0 35.0 40.5 20.0 41.0 54-55 34.30119773042399 39.0 35.0 40.0 18.5 41.0 56-57 34.05020038191151 38.5 34.5 40.0 13.0 41.0 58-59 33.8155756646963 38.0 34.0 40.0 8.0 41.0 60-61 33.50616045148264 38.0 34.0 40.0 4.0 41.0 62-63 33.174616568148096 38.0 33.5 40.0 2.0 41.0 64-65 32.81714162348877 37.5 33.0 40.0 2.0 41.0 66-67 32.391544430659955 37.0 33.0 39.5 2.0 41.0 68-69 31.937389014571018 36.0 32.0 39.0 2.0 40.5 70-71 31.532635797513926 36.0 31.5 39.0 2.0 40.0 72-73 31.047849923374443 35.5 31.0 38.5 2.0 40.0 74-75 30.522394731080787 35.0 31.0 37.5 2.0 39.5 76-77 29.211477413705026 34.0 28.5 36.0 2.0 39.0 78-79 29.743495949792013 34.5 30.0 36.0 2.0 39.0 80-81 29.494319978593495 34.5 30.0 36.0 2.0 38.5 82-83 29.10051783064536 34.0 29.5 36.0 2.0 37.5 84-85 28.662579970322803 34.0 29.0 35.0 2.0 37.0 86-87 28.235886606339243 34.0 29.0 35.0 2.0 36.5 88-89 27.832544272543725 34.0 28.5 35.0 2.0 36.0 90-91 27.332291894718917 34.0 26.5 35.0 2.0 36.0 92-93 26.894034761244498 33.5 26.0 35.0 2.0 35.0 94-95 26.428727590065435 33.0 25.0 35.0 2.0 35.0 96-97 25.921557627283562 33.0 24.5 35.0 2.0 35.0 98-99 25.12013336495658 32.5 18.5 34.5 2.0 35.0 100-101 22.703784451093433 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26216007421616183 1101 2 0.13460502708741018 1101 3 0.2672731359177405 1101 4 0.17090787144482533 1101 5 0.1304682985586254 1101 6 0.16303837521216735 1101 7 0.1857070815733053 1101 8 -0.06368919498808623 1101 9 0.13732160148278894 1101 10-11 0.2019906458360765 1101 12-13 0.24142222838357696 1101 14-15 0.15915317517423944 1101 16-17 0.30079960764333435 1101 18-19 0.3149413255188449 1101 20-21 0.18373394670911836 1101 22-23 0.3213999514952519 1101 24-25 0.34311207567176183 1101 26-27 0.15987697031347636 1101 28-29 0.3078204557036557 1101 30-31 0.47148335859744606 1101 32-33 0.4041388317454562 1101 34-35 0.4201330996781678 1101 36-37 0.3550968173917042 1101 38-39 0.387386588495751 1101 40-41 0.6306029205939652 1101 42-43 0.8250984623472704 1101 44-45 0.8791685896524157 1101 46-47 1.3393888072744957 1101 48-49 1.244981757205224 1101 50-51 1.0382666693747922 1101 52-53 0.9244982915070636 1101 54-55 1.155110800712933 1101 56-57 0.9439669715327028 1101 58-59 1.003988987056914 1101 60-61 0.8844784601808513 1101 62-63 0.9921426289907842 1101 64-65 0.9710294290121695 1101 66-67 0.8794180016885065 1101 68-69 0.9291656597669835 1101 70-71 1.1265919785373235 1101 72-73 1.1095805204521376 1101 74-75 0.9841463086962392 1101 76-77 0.9443511269333875 1101 78-79 0.8484088516503334 1101 80-81 0.9122927044949876 1101 82-83 1.1334593801285102 1101 84-85 1.390019114005053 1101 86-87 1.371594804748593 1101 88-89 1.0351085519192615 1101 90-91 0.821228611364095 1101 92-93 0.9603518833082596 1101 94-95 1.0706065273453795 1101 96-97 1.1730408500755871 1101 98-99 1.4325470047221955 1101 100-101 1.64257698270578 1102 1 0.36599865454695646 1102 2 0.38580771232954447 1102 3 0.24255670263637796 1102 4 0.25413035271917295 1102 5 0.166679971161237 1102 6 0.2186265109427623 1102 7 0.3447142285512186 1102 8 0.27531308880271865 1102 9 0.3665016776727228 1102 10-11 0.3469684515851341 1102 12-13 0.17909238033433184 1102 14-15 0.5267076118789049 1102 16-17 0.3839291162230509 1102 18-19 0.4082692235254157 1102 20-21 0.4571857129519259 1102 22-23 0.39140362272473084 1102 24-25 0.35533202522810825 1102 26-27 0.4016798777365125 1102 28-29 0.6395351105842408 1102 30-31 0.6744713579680877 1102 32-33 0.958527858106784 1102 34-35 0.8755062062534904 1102 36-37 0.9695009100974019 1102 38-39 1.185883768324011 1102 40-41 1.0944484619572847 1102 42-43 1.332631634527928 1102 44-45 1.475597961395387 1102 46-47 1.5797947403627646 1102 48-49 1.7394081633576377 1102 50-51 1.9196573501733099 1102 52-53 2.403052589944643 1102 54-55 2.423158231090696 1102 56-57 2.137769745456488 1102 58-59 2.3559430823849183 1102 60-61 2.2007405764300927 1102 62-63 2.1870753787416746 1102 64-65 2.4961129852415738 1102 66-67 2.4148000717851446 1102 68-69 2.7359850877226854 1102 70-71 2.6587724504571817 1102 72-73 2.6359084804405057 1102 74-75 2.5804135742024137 1102 76-77 2.509429629262474 1102 78-79 2.627503345282893 1102 80-81 2.8401363577974266 1102 82-83 2.3856680776048975 1102 84-85 2.6015296103838885 1102 86-87 2.5136809213029743 1102 88-89 2.6280311843338353 1102 90-91 2.4466487764263896 1102 92-93 2.709943041843392 1102 94-95 2.998857830155064 1102 96-97 2.9033246790249017 1102 98-99 2.8766799509076897 1102 100-101 3.1528302021833206 1103 1 0.23955092404406741 1103 2 0.3743014678105112 1103 3 0.3814481115087318 1103 4 0.06531303136080169 1103 5 0.11793341093867582 1103 6 0.1891880640589747 1103 7 0.18587967050591914 1103 8 0.25804616207375375 1103 9 0.19792990350355666 1103 10-11 0.2109800636689485 1103 12-13 0.2024072673886721 1103 14-15 0.25733901893687516 1103 16-17 0.3393707685258036 1103 18-19 0.39358023988514645 1103 20-21 0.30344656148076155 1103 22-23 0.21416058543905336 1103 24-25 0.3107812374812795 1103 26-27 0.2274381407623025 1103 28-29 0.36813289098279967 1103 30-31 0.5482731058286205 1103 32-33 0.68917438506557 1103 34-35 0.6631925590310175 1103 36-37 0.544737536545135 1103 38-39 0.651765712138463 1103 40-41 0.4218245543701187 1103 42-43 0.793206493156859 1103 44-45 0.779182889878264 1103 46-47 1.0665799470518067 1103 48-49 1.2088810836152817 1103 50-51 1.3331866374211927 1103 52-53 1.2834314946590268 1103 54-55 1.2100507890755097 1103 56-57 1.3229204908676024 1103 58-59 1.5435734915294361 1103 60-61 1.5543799365558897 1103 62-63 1.5224196588831944 1103 64-65 1.5815479847879814 1103 66-67 1.5451033769954634 1103 68-69 1.446227308533718 1103 70-71 1.3210997055903881 1103 72-73 1.4584764443946625 1103 74-75 1.6634218296682626 1103 76-77 1.6291716837545955 1103 78-79 1.4992639065336135 1103 80-81 1.2702446162872505 1103 82-83 1.5058939689230506 1103 84-85 1.444894997906946 1103 86-87 1.7937695239217426 1103 88-89 1.8741151300231529 1103 90-91 1.9120695632660265 1103 92-93 1.9481646391097094 1103 94-95 1.9792699986512368 1103 96-97 1.6742884215027516 1103 98-99 2.1117263690031898 1103 100-101 2.142261391514719 1104 1 0.1309996579585544 1104 2 0.1494415674369094 1104 3 -0.0011406182546522814 1104 4 -0.06213871210121624 1104 5 -0.0724204703398641 1104 6 0.09546400843423442 1104 7 -0.03152122198059004 1104 8 0.013598258379275308 1104 9 0.060009604624355006 1104 10-11 0.08051877267683949 1104 12-13 0.058714345014244884 1104 14-15 0.18138037427103626 1104 16-17 -0.0382625855630252 1104 18-19 -0.10514199963332516 1104 20-21 0.053187117728164424 1104 22-23 -0.11965271883658346 1104 24-25 0.10687790622349524 1104 26-27 0.07325071859543186 1104 28-29 -0.08135341038705945 1104 30-31 0.38919516892534745 1104 32-33 0.3451454830315299 1104 34-35 0.39485637181640243 1104 36-37 0.3018527814600347 1104 38-39 0.4907484331845424 1104 40-41 0.7209567150176852 1104 42-43 0.8872548534723421 1104 44-45 0.8987021406047049 1104 46-47 0.7828068565578192 1104 48-49 0.3267269221374818 1104 50-51 0.48285973829291606 1104 52-53 0.3371817541027866 1104 54-55 0.6432440227824401 1104 56-57 0.6936785855126786 1104 58-59 0.3840480141529312 1104 60-61 0.5450205031810427 1104 62-63 0.5991890652758869 1104 64-65 0.618551150001764 1104 66-67 0.28626931721962734 1104 68-69 0.47570011882844554 1104 70-71 0.5787597823857453 1104 72-73 0.3211852966155 1104 74-75 0.20250054490279723 1104 76-77 0.21305633506219124 1104 78-79 0.538666926458891 1104 80-81 0.37103202807637814 1104 82-83 0.7061326571754378 1104 84-85 0.47563908258938525 1104 86-87 0.6635028157440779 1104 88-89 0.25270193990276724 1104 90-91 0.623217861315009 1104 92-93 0.42688376530315253 1104 94-95 0.5625838737031259 1104 96-97 1.0744220350353366 1104 98-99 1.257358633428261 1104 100-101 1.4783902312822583 1105 1 0.18003894674547638 1105 2 0.16931896921494172 1105 3 -0.0029777992124948582 1105 4 0.023957860884152637 1105 5 0.13834451388726876 1105 6 0.01745103166337003 1105 7 0.1561849007610263 1105 8 0.0520625543356843 1105 9 0.18196257279183214 1105 10-11 0.0848115028173595 1105 12-13 0.26622617466724563 1105 14-15 0.12865328078093796 1105 16-17 0.20995400462911817 1105 18-19 0.05737682026916957 1105 20-21 -0.08801592213417564 1105 22-23 0.22988664164520856 1105 24-25 0.11352312417589161 1105 26-27 0.03727781925502427 1105 28-29 0.15070494923438815 1105 30-31 -0.11015061541650084 1105 32-33 -0.16457365232838583 1105 34-35 -0.14501941682712527 1105 36-37 -0.15390700488925546 1105 38-39 -0.08295837065830369 1105 40-41 0.11477213658350394 1105 42-43 -0.042968117562935504 1105 44-45 -0.058482581219216456 1105 46-47 0.303100984748248 1105 48-49 0.45456335029922457 1105 50-51 0.5507010058581585 1105 52-53 0.44583084357797986 1105 54-55 0.3879565263671907 1105 56-57 0.5896224484320953 1105 58-59 0.4931183109696775 1105 60-61 0.37405065087246925 1105 62-63 0.39670483814655455 1105 64-65 0.4495708302426564 1105 66-67 0.6501118035976035 1105 68-69 0.3561130812603359 1105 70-71 0.6989427835328605 1105 72-73 0.8831699897614698 1105 74-75 0.777497856345299 1105 76-77 0.7866735203217274 1105 78-79 1.2883935897797976 1105 80-81 1.207473632607492 1105 82-83 1.5071435547951673 1105 84-85 1.2322529340376214 1105 86-87 1.5404565008602198 1105 88-89 1.2659341237284103 1105 90-91 1.089064219215782 1105 92-93 1.187310887471206 1105 94-95 1.0553650968175496 1105 96-97 1.5142117898388925 1105 98-99 1.4231037223298948 1105 100-101 0.9729010930545527 1106 1 -0.09357946662056804 1106 2 0.25512338561873094 1106 3 0.18692756356352902 1106 4 0.06759613638362794 1106 5 0.03822767780828684 1106 6 0.12267360439383168 1106 7 -0.10990926911863852 1106 8 -0.09456672478570738 1106 9 -0.05194325732850302 1106 10-11 -0.13031876941069953 1106 12-13 -0.12207269202279036 1106 14-15 0.07190015541581829 1106 16-17 -0.011263427313870977 1106 18-19 -0.009108750475071759 1106 20-21 -0.007334767793722108 1106 22-23 -0.14791450334836043 1106 24-25 -0.006747935527350535 1106 26-27 0.03583489367960624 1106 28-29 -0.30033489186853757 1106 30-31 -0.17572486474468718 1106 32-33 0.06068630794734986 1106 34-35 -0.008268628183600413 1106 36-37 0.10036919560145208 1106 38-39 0.13768656449767747 1106 40-41 0.19247397090994411 1106 42-43 -0.09763571891823375 1106 44-45 0.09980693858449996 1106 46-47 0.8860370754130429 1106 48-49 1.5604285214640825 1106 50-51 1.5723799403131267 1106 52-53 1.3459885722845968 1106 54-55 1.6495992416376595 1106 56-57 1.5165531646372585 1106 58-59 1.3530610612909797 1106 60-61 1.42817496446051 1106 62-63 1.5042816578684821 1106 64-65 1.5680776651532753 1106 66-67 1.7006843003846015 1106 68-69 1.8172826104109383 1106 70-71 1.9614091931601543 1106 72-73 1.8965535626087657 1106 74-75 2.0880539960812463 1106 76-77 2.255554230685089 1106 78-79 2.12765051231748 1106 80-81 2.260604839524188 1106 82-83 2.073220199262977 1106 84-85 2.303038072488377 1106 86-87 1.7943529840942425 1106 88-89 1.792379970205804 1106 90-91 2.2100655044126505 1106 92-93 2.361279809074194 1106 94-95 2.276378065622321 1106 96-97 2.413405620893922 1106 98-99 2.7225838017784305 1106 100-101 2.528106140373165 1107 1 0.3249390518979496 1107 2 0.2932186237139689 1107 3 0.22026089689686046 1107 4 0.21521518400268036 1107 5 0.051984291564899365 1107 6 0.15441963613986331 1107 7 0.21601665680729099 1107 8 0.0985549683359892 1107 9 0.21736891198366237 1107 10-11 0.3868769977850661 1107 12-13 0.2863929164428143 1107 14-15 0.30179433530999944 1107 16-17 0.125509059458615 1107 18-19 0.31813992677360403 1107 20-21 0.46541655495759926 1107 22-23 0.3454717400378797 1107 24-25 0.39907217029276154 1107 26-27 0.35620526404997577 1107 28-29 0.19543230389865585 1107 30-31 0.3237460347262058 1107 32-33 0.111744509005554 1107 34-35 0.2298266099116404 1107 36-37 -0.1226466774144157 1107 38-39 0.1845119613230679 1107 40-41 0.10173323016920932 1107 42-43 0.026702905420393108 1107 44-45 0.2061561449337077 1107 46-47 -0.8367142473382856 1107 48-49 -1.176343965308405 1107 50-51 -1.4101597422265613 1107 52-53 -1.7754399991439698 1107 54-55 -1.7207711287327072 1107 56-57 -1.418632020547932 1107 58-59 -1.3252458170159045 1107 60-61 -1.135052548767007 1107 62-63 -1.4311680775812547 1107 64-65 -1.1247794777038678 1107 66-67 -1.0924373927370858 1107 68-69 -0.7316591885308625 1107 70-71 -0.8740405422895812 1107 72-73 -0.8021236860684837 1107 74-75 -0.9553322473049946 1107 76-77 -0.9870383619075049 1107 78-79 -0.9112383765714078 1107 80-81 -1.1904533615340114 1107 82-83 -1.1415946155518348 1107 84-85 -0.6152158957655907 1107 86-87 -0.17204913230787255 1107 88-89 -0.4933343155084877 1107 90-91 -0.3222096278624811 1107 92-93 -0.500889503095113 1107 94-95 -0.2315584423141921 1107 96-97 -0.7254832679949672 1107 98-99 -0.41286593367130564 1107 100-101 -0.4623700501030257 1108 1 0.11447073233871663 1108 2 0.27082586495757255 1108 3 0.09932425777840592 1108 4 0.1116732713698596 1108 5 0.1429720916436068 1108 6 0.14324293405407218 1108 7 0.2471461333913112 1108 8 0.08694536581729295 1108 9 0.2480261335499776 1108 10-11 0.2006117478834497 1108 12-13 0.1302536012125941 1108 14-15 0.3919950436925248 1108 16-17 0.06478493509813177 1108 18-19 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2316 82-83 0.3733225115199872 2316 84-85 0.29284777169034015 2316 86-87 0.2607843325697914 2316 88-89 0.10830998862200758 2316 90-91 0.6913525926058206 2316 92-93 -0.17181820606801423 2316 94-95 -0.500724451259206 2316 96-97 -0.1046729549194616 2316 98-99 0.14564498450812025 2316 100-101 0.9507580740748232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 7.0 6 12.0 7 40.0 8 117.0 9 218.0 10 419.0 11 701.0 12 1239.0 13 1776.0 14 2324.0 15 3436.0 16 4925.0 17 6965.0 18 5883.0 19 3122.0 20 2183.0 21 2066.0 22 2233.0 23 2443.0 24 2613.0 25 2993.0 26 3217.0 27 3723.0 28 4496.0 29 5534.0 30 6712.0 31 8144.0 32 10234.0 33 13294.0 34 18542.0 35 27390.0 36 39050.0 37 68478.0 38 67361.0 39 6980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.508950893264476 23.08649184595826 17.15329355158549 19.251263709191775 2 14.306781969884941 23.430392371500158 41.820221849230094 20.442603809384806 3 17.00327178963244 28.584373251599406 33.9314383711596 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37.203111269228785 16.864921898993217 24-25 17.04449914328241 29.276632362488087 37.28857623722335 16.390292257006156 26-27 16.75066629569998 29.151292073931835 37.872962153796095 16.225079476572084 28-29 16.269466604026885 29.465174866852458 37.942222404668186 16.32313612445247 30-31 16.976361623975286 29.20452334038775 37.43812182247196 16.380993213165002 32-33 16.479359750906127 29.48928458488409 37.39920090491133 16.63215475929845 34-35 17.039608115011312 29.417676177965895 37.04876061203143 16.493955094991364 36-37 17.43064170862828 29.049143739813665 36.70516188669148 16.815052664866574 38-39 16.30332819961596 30.19811689368036 36.813770728080726 16.68478417862296 40-41 16.703282000915255 29.81288930715679 36.878001760576765 16.60582693135119 42-43 16.92258331356556 29.422051104258284 36.51412027322105 17.141245308955106 44-45 17.374692889634876 29.448083144810138 35.868970298474785 17.3082536670802 46-47 17.519886156316137 29.78408012843903 35.4111934126347 17.28484030261013 48-49 17.363898416405167 30.106393976988006 34.81035174779245 17.719355858814374 50-51 17.277086526064853 30.15808582062322 34.58777275049259 17.977054902819333 52-53 17.557438760368775 30.955660560947724 33.95287528278479 17.53402539589871 54-55 17.798413972609403 30.035697779075143 33.944817436571064 18.221070811744386 56-57 18.17378797830159 30.524641805930575 33.35416210562164 17.947408110146196 58-59 18.100355153372742 30.390547082147464 33.35264175727943 18.15645600720037 60-61 18.052160110924614 30.46428397674475 33.745651803741275 17.737904108589362 62-63 17.660062273468096 30.277129095818434 33.854356710209444 18.208451920504025 64-65 17.596967817266293 30.049989053491938 34.13258045683427 18.220462672407503 66-67 17.623269843586563 30.425667128852563 33.12489357561604 18.82616945194483 68-69 17.902433017151075 29.743227978100865 33.187886454131785 19.166452550616274 70-71 17.97279792746114 29.939459729013112 33.693959960106056 18.393782383419687 72-73 17.813617456031526 29.477121798146396 34.04835315867572 18.660907587146365 74-75 17.631175654966068 30.406814809409134 33.040362207789045 18.921647327835753 76-77 17.369979809774016 29.867547252426473 33.684685835218566 19.077787102580942 78-79 17.403883577805345 30.06686492009049 32.82508088253181 19.704170619572356 80-81 17.240294096183316 29.6606278430514 32.91022038969569 20.188857671069595 82-83 17.031835106342545 30.136848842919544 33.00442580804065 19.826890242697264 84-85 17.167369576520887 29.48602947660329 32.81590654086253 20.5306944060133 86-87 16.812772142353253 29.27035442360554 32.80698873725948 21.109884696781727 88-89 17.161235982388284 28.89087547738938 33.050396506847655 20.897492033374686 90-91 16.619504147311282 28.766298439151228 33.66035383293672 20.95384358060077 92-93 16.69454056716799 28.35049352007833 33.874239042516315 21.080726870237363 94-95 16.290228417134934 28.17418326887056 34.01688194799192 21.51870636600258 96-97 16.897151475346032 28.406644530394804 32.96829769636819 21.72790629789097 98-99 16.42084906277129 28.710259571834946 32.5719854592337 22.29690590616006 100-101 15.754145427559946 27.773300161802457 33.75162714874877 22.720927261888832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1113.0 1 722.5 2 414.5 3 499.0 4 392.0 5 413.0 6 797.5 7 1481.0 8 2095.5 9 2206.5 10 1790.5 11 1361.0 12 1273.0 13 1235.5 14 1170.0 15 1085.0 16 1070.0 17 1099.0 18 1117.5 19 1229.5 20 1384.0 21 1564.0 22 1807.5 23 2087.5 24 2413.5 25 3047.0 26 4234.0 27 5541.5 28 6060.0 29 6692.0 30 7935.0 31 8873.0 32 10111.0 33 11574.0 34 14074.0 35 16312.5 36 17555.0 37 18551.5 38 17862.5 39 16191.5 40 14483.5 41 13068.0 42 11714.5 43 11016.5 44 10731.0 45 10154.5 46 9137.5 47 8063.5 48 7980.5 49 7145.5 50 5555.0 51 4434.0 52 3580.5 53 3062.5 54 2626.0 55 2233.5 56 1848.5 57 1507.0 58 1212.5 59 933.0 60 707.5 61 549.0 62 395.5 63 250.5 64 166.0 65 113.5 66 75.5 67 62.5 68 48.0 69 33.5 70 29.0 71 20.5 72 14.5 73 10.5 74 8.0 75 7.0 76 6.0 77 2.5 78 1.5 79 0.5 80 1.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023717434138509817 2 0.0 3 0.0 4 3.0406966844243353E-4 5 0.02158894645941278 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0028886618502031182 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.122090053273005E-4 24-25 1.5203483422121677E-4 26-27 4.5610450266365027E-4 28-29 0.0022805225133182517 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.5203483422121677E-4 40-41 7.601741711060838E-4 42-43 0.01641976209589141 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.5203483422121677E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016723831764333843 84-85 3.0406966844243353E-4 86-87 0.0 88-89 0.0 90-91 0.013531100245688292 92-93 0.003040696684424335 94-95 0.0 96-97 0.0 98-99 0.0010642438395485174 100-101 0.023109294801624946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 328872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.61724762539087 #Duplication Level Percentage of deduplicated Percentage of total 1 83.1032496730177 39.57148018154766 2 9.16211630582329 8.725495218140379 3 2.5617442169981164 3.6594962620113707 4 1.133842039465578 2.159617486452425 5 0.6693527972021011 1.5936368946560242 6 0.4874456838580047 1.392649309931676 7 0.34269753520449264 1.14228193761104 8 0.2607416290490701 0.9932638973341903 9 0.20220426682016593 0.8665569579677802 >10 1.7538265597998017 17.115431485267603 >50 0.20406325069225634 6.704987498071368 >100 0.10844870870123056 8.732403623929432 >500 0.00705879169061264 2.191503911779057 >1k 0.0025668333420409604 3.4534826005196466 >5k 6.417083355102401E-4 1.697712734780318 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5556 1.6894110778661608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4640 1.4108832615728917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 1.1353961419640468 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1738 0.5284730837529495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1190 0.3618429054464959 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 882 0.26818944756622637 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 730 0.22197085796297647 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 718 0.21832202194166728 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 701 0.2131528375781459 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 678 0.20615923520396992 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.19095575178184826 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 589 0.17909703471259336 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 576 0.1751441290228417 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.1742319200175144 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 572 0.17392785034907196 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 525 0.15963657593227762 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 498 0.1514266948843319 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 489 0.14869006786835 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 487 0.14808192853146512 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 379 0.1152424043396823 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 353 0.10733659296017904 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 339 0.10307961760198496 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 335 0.10186333892821524 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.081393368848671E-4 0.0 20-21 0.0 0.0 0.0 6.081393368848671E-4 0.0 22-23 0.0 0.0 0.0 9.122090053273006E-4 0.0 24-25 0.0 0.0 0.0 0.0024325573475394682 0.0 26-27 0.0 0.0 0.0 0.0031927315186455517 0.0 28-29 0.0 0.0 0.0 0.00653749787151232 0.0 30-31 0.0 0.0 0.0 0.013531100245688293 0.0 32-33 0.0 0.0 0.0 0.021740981293634 0.0 34-35 0.0 0.0 0.0 0.028582548833588753 0.0 36-37 0.0 0.0 0.0 0.03740056921841932 0.0 38-39 0.0 0.0 0.0 0.0516918436352137 0.0 40-41 0.0 0.0 0.0 0.07997032280036001 0.0 42-43 0.0 0.0 0.0 0.1278612955800433 0.0 44-45 0.0 0.0 0.0 0.176056338028169 0.0 46-47 0.0 0.0 0.0 0.28552141866744507 0.0 48-49 0.0 0.0 0.0 0.3928580116276241 0.0 50-51 0.0 0.0 0.0 0.47009170741200224 0.0 52-53 0.0 0.0 0.0 0.5613126079447324 0.0 54-55 0.0 0.0 0.0 0.6344413632051376 0.0 56-57 0.0 0.0 0.0 0.8447055389330804 0.0 58-59 0.0 0.0 0.0 1.0887214478581333 0.0 60-61 0.0 0.0 0.0 1.3231591622272494 0.0 62-63 0.0 0.0 0.0 1.5835948332481937 0.0 64-65 0.0 0.0 0.0 1.921112165219295 0.0 66-67 0.0 0.0 0.0 2.3519788854022234 0.0 68-69 0.0 0.0 0.0 2.837882215573232 0.0 70-71 0.0 0.0 0.0 3.226331217008441 0.0 72-73 0.0 0.0 0.0 3.5337456518037413 0.0 74-75 0.0 0.0 0.0 3.9375501714952934 0.0 76-77 0.0 0.0 0.0 4.475449414969958 0.0 78-79 0.0 0.0 0.0 4.985982388284803 0.0 80-81 0.0 0.0 0.0 5.48283222651974 0.0 82-83 0.0 0.0 0.0 6.067862268602982 0.0 84-85 0.0 0.0 0.0 6.940390182198545 0.0 86-87 0.0 0.0 0.0 7.985629667469411 0.0 88-89 0.0 0.0 0.0 9.070094139969349 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTCGC 25 0.005020514 56.993065 7 GGTATCA 1785 0.0 44.181732 1 CATTCCG 155 0.0 42.898006 9 GTATCAA 3045 0.0 37.289234 1 TTAGGCA 230 0.0 37.16939 4 CCTTTAG 205 0.0 37.079937 1 GGCATTC 200 0.0 33.245956 7 TATGAGA 135 1.19307515E-8 31.662815 4 GTATAGG 170 1.07320375E-10 30.740904 1 GCATTCC 220 0.0 30.223595 8 TAGGCAT 220 0.0 30.223595 5 CTACACG 65 0.0058415574 29.227215 4 CTTTAGG 235 0.0 28.29443 2 CTGGTCG 85 6.4019323E-4 27.937778 9 TATCAAC 4120 0.0 27.320707 2 TCAACGC 4130 0.0 27.254555 4 ATCAACG 4145 0.0 27.155926 3 TTGATCG 70 0.008387989 27.139557 9 TACACGG 70 0.008387989 27.139557 5 CAACGCA 4220 0.0 26.673296 5 >>END_MODULE