FastQCFastQC Report
Thu 26 May 2016
SRR937645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302956
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44741.4767821069726297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34261.130857286206578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24670.8143096687307727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17940.5921651989067719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.25878345370284794No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.22412495543907368No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5630.18583556688099923No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5550.18319491939423546No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5150.1699916819604167No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.15348763516814323No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4370.1442453689644701No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4210.13896407399094257No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA4210.13896407399094257No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.13830391211925164No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4060.13401285995326054No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4050.13368277901741507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4010.13236245527403318No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT3970.1310421315306513No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC3940.1300518887231149No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.11948929877605989No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.11915921784021442No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT3510.11585840848175973No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3280.10826654695731393No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA3270.10793646602146846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG751.3559838E-744.3431439
GGTATCA18700.036.6221
CTCTAGG550.00256548734.575992
GTATCAA29900.033.560811
TTAGGCA1001.2927576E-633.2573554
GGACCTG753.0691325E-431.6736746
TAGGCAT1102.7164588E-630.2389665
GTGCAGG804.4540784E-429.7235851
CATGGGG8600.027.622394
ATCAACG36650.027.2274463
AGGCTTA1754.9185473E-927.1488656
GAAGCGT700.00837291327.148866
AAGCGTG700.00837291327.148867
GGCGAGA350.00838619727.14437118-19
TATCAAC36800.027.1299362
TCAACGC36950.027.0019154
CAACGCA37050.026.933495
GTACATG21150.026.7582531
AACGCAG37300.026.7485456
ATGGGGG3450.026.169545
Produced by FastQC (version 0.11.5)