##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937645_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302956 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.999584098020836 33.0 31.0 34.0 30.0 34.0 2 32.125602397707915 33.0 31.0 34.0 30.0 34.0 3 32.10799588058992 34.0 31.0 34.0 30.0 34.0 4 35.718289124493324 37.0 35.0 37.0 35.0 37.0 5 35.743923209971086 37.0 35.0 37.0 35.0 37.0 6 35.821475725847975 37.0 35.0 37.0 35.0 37.0 7 35.78994309404666 37.0 35.0 37.0 35.0 37.0 8 35.783499914178954 37.0 35.0 37.0 35.0 37.0 9 37.42150345264659 39.0 37.0 39.0 35.0 39.0 10-11 37.35196200108267 39.0 37.0 39.0 35.0 39.0 12-13 37.26686218460766 39.0 37.0 39.0 34.0 39.0 14-15 38.41050020465018 40.0 38.0 41.0 34.0 41.0 16-17 38.37783539523891 40.0 38.0 41.0 34.0 41.0 18-19 38.44695269280028 40.0 38.0 41.0 34.0 41.0 20-21 38.44903055229142 40.0 38.0 41.0 34.0 41.0 22-23 38.281304875955584 40.0 38.0 41.0 34.0 41.0 24-25 38.13652972708908 40.0 38.0 41.0 34.0 41.0 26-27 38.05379329011474 40.0 38.0 41.0 34.0 41.0 28-29 38.04416152840676 40.0 38.0 41.0 34.0 41.0 30-31 37.79731215093941 40.0 37.0 41.0 34.0 41.0 32-33 37.47150741361782 40.0 36.0 41.0 33.0 41.0 34-35 37.391243282852955 40.0 36.0 41.0 33.0 41.0 36-37 37.2518352500033 40.0 36.0 41.0 33.0 41.0 38-39 37.079176514081254 40.0 35.0 41.0 33.0 41.0 40-41 36.848552265015385 40.0 35.0 41.0 32.5 41.0 42-43 36.53089227478578 39.0 35.0 41.0 31.0 41.0 44-45 36.16750947332286 39.0 35.0 41.0 30.5 41.0 46-47 35.65666466417565 39.0 35.0 41.0 28.5 41.0 48-49 35.193384517883786 39.0 35.0 41.0 25.0 41.0 50-51 34.22308685089584 38.0 34.0 40.0 23.0 40.5 52-53 34.29319439126474 38.5 35.0 40.0 23.0 40.5 54-55 34.63434294088911 39.0 35.0 40.0 22.5 41.0 56-57 34.480046607428136 39.0 35.0 41.0 19.5 41.0 58-59 34.26549729993795 39.0 35.0 40.0 16.5 41.0 60-61 33.976116993886905 38.5 34.5 40.0 10.5 41.0 62-63 33.65138336920213 38.0 34.0 40.0 7.0 41.0 64-65 33.3309209918272 38.0 34.0 40.0 2.0 41.0 66-67 32.93827816580625 37.5 33.5 40.0 2.0 41.0 68-69 32.44386478564544 37.0 33.0 39.5 2.0 41.0 70-71 32.04754650840386 36.0 32.5 39.0 2.0 41.0 72-73 31.59994685696933 36.0 32.0 39.0 2.0 40.0 74-75 31.117599585418347 35.0 31.5 38.0 2.0 40.0 76-77 30.61496388914562 35.0 31.0 37.5 2.0 39.5 78-79 30.08315563976287 35.0 30.5 37.0 2.0 39.0 80-81 29.53154088382471 35.0 30.0 36.0 2.0 39.0 82-83 29.017829321749694 34.0 29.0 36.0 2.0 37.5 84-85 28.539144298181917 34.0 29.0 35.5 2.0 37.0 86-87 28.074352381203873 34.0 28.5 35.0 2.0 36.5 88-89 27.602972048746352 34.0 27.0 35.0 2.0 36.0 90-91 27.141268038923144 34.0 26.0 35.0 2.0 36.0 92-93 26.65582295778925 34.0 25.5 35.0 2.0 35.0 94-95 26.103305430491556 33.0 24.5 35.0 2.0 35.0 96-97 25.483220335626296 33.0 20.0 35.0 2.0 35.0 98-99 24.687355589590567 32.0 8.5 35.0 2.0 35.0 100-101 22.536181821782698 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1505360783049916 1101 2 0.5126877160798387 1101 3 0.4138018631291587 1101 4 0.4258846677947403 1101 5 0.40995270744571854 1101 6 0.31186159862279794 1101 7 0.3151628522806931 1101 8 0.33571454674603274 1101 9 0.42213846632482444 1101 10-11 0.28784335712291664 1101 12-13 0.4862254210682053 1101 14-15 0.6714878307542165 1101 16-17 0.6163175101418616 1101 18-19 0.5429521494956688 1101 20-21 0.5860202688675145 1101 22-23 0.4701295249992228 1101 24-25 0.2455538756554887 1101 26-27 0.4358244604157875 1101 28-29 0.36430345218653315 1101 30-31 0.4887126487597655 1101 32-33 0.34858105166426157 1101 34-35 0.7278217876389874 1101 36-37 0.5952994314630686 1101 38-39 0.7629276802900762 1101 40-41 0.841035037879756 1101 42-43 0.6372263795571342 1101 44-45 0.8648130801255576 1101 46-47 1.0031871714508327 1101 48-49 1.1488592275523501 1101 50-51 1.1559654827979742 1101 52-53 1.3836748200592552 1101 54-55 1.6959259215873104 1101 56-57 1.6794808723631078 1101 58-59 1.6489145341599638 1101 60-61 1.611778578165449 1101 62-63 1.948834011398226 1101 64-65 1.8210322460463857 1101 66-67 2.1842979072317235 1101 68-69 2.0850222889178696 1101 70-71 2.000470120112009 1101 72-73 2.0178883049816285 1101 74-75 2.06284688731267 1101 76-77 1.9560839403434294 1101 78-79 1.9555786862702327 1101 80-81 1.9388040861490872 1101 82-83 2.1612683374330786 1101 84-85 1.878208651398758 1101 86-87 1.9686617128228328 1101 88-89 2.0041624605032347 1101 90-91 2.0917227561639287 1101 92-93 2.179688233375007 1101 94-95 2.104421519814281 1101 96-97 2.2974898758825084 1101 98-99 2.422093961087917 1101 100-101 3.1308637294922264 1102 1 0.2374203885319055 1102 2 -0.052582213012030365 1102 3 -0.044303722621336306 1102 4 0.0027138044021555174 1102 5 0.19000381888820073 1102 6 0.1800105314014786 1102 7 0.03691401051534626 1102 8 0.018967579238307053 1102 9 0.09230511842250166 1102 10-11 0.009450539463252028 1102 12-13 -0.10585744734296298 1102 14-15 0.24581538619779053 1102 16-17 0.3664319195578898 1102 18-19 0.27394090394992077 1102 20-21 0.38090089742513555 1102 22-23 0.4639591295282912 1102 24-25 0.44587576461922396 1102 26-27 0.3744778744441817 1102 28-29 0.3680519674351217 1102 30-31 0.4633651783648034 1102 32-33 0.20242495094284152 1102 34-35 0.5793517886159592 1102 36-37 0.6852303744537593 1102 38-39 0.7750010874281941 1102 40-41 0.9676001329809694 1102 42-43 0.993260769227895 1102 44-45 1.0888061487146459 1102 46-47 1.0683028406559316 1102 48-49 1.07355469282588 1102 50-51 0.8964617882736903 1102 52-53 0.9873914549312417 1102 54-55 1.1067302840569155 1102 56-57 1.1661965251138255 1102 58-59 0.9147248870551579 1102 60-61 1.3003432927589458 1102 62-63 1.5434004639864511 1102 64-65 1.5169114502710528 1102 66-67 1.709030909077697 1102 68-69 1.4572128307300076 1102 70-71 1.356766751590289 1102 72-73 1.346520996765225 1102 74-75 1.2927918165330858 1102 76-77 1.3145864882025613 1102 78-79 1.5217870296733054 1102 80-81 1.3700967325802829 1102 82-83 1.237714395321671 1102 84-85 1.2539934485727144 1102 86-87 1.5712856734968454 1102 88-89 1.4890244236145556 1102 90-91 1.607968893239267 1102 92-93 2.0902174990465276 1102 94-95 2.254652652544628 1102 96-97 2.5195032759246736 1102 98-99 2.6293652579507842 1102 100-101 3.1500665143973805 1103 1 0.34127582320387617 1103 2 0.4077168092080612 1103 3 0.3795366232241477 1103 4 0.4662102208058627 1103 5 0.1600297453241808 1103 6 0.37911717707152803 1103 7 0.23053598751087634 1103 8 0.4098340491115451 1103 9 0.35397051770758026 1103 10-11 0.3265284564866775 1103 12-13 0.40608487980913566 1103 14-15 0.6598958958648211 1103 16-17 0.5880400771043952 1103 18-19 0.4445853525191765 1103 20-21 0.5195803856025591 1103 22-23 0.579861674869619 1103 24-25 0.29990334364096327 1103 26-27 0.3416237510630893 1103 28-29 0.3504064231296553 1103 30-31 0.7189471460196302 1103 32-33 0.8182915705068226 1103 34-35 0.7051937990224602 1103 36-37 0.8346606515013804 1103 38-39 0.9939567250808068 1103 40-41 1.1525490285626958 1103 42-43 1.0444939215352278 1103 44-45 1.145188662441285 1103 46-47 0.9014186234486772 1103 48-49 1.1251950039431122 1103 50-51 1.1019392717927374 1103 52-53 0.9581568536175595 1103 54-55 1.098344608861865 1103 56-57 1.253663817687361 1103 58-59 1.1590291544387483 1103 60-61 1.3623631597138157 1103 62-63 1.699920094429956 1103 64-65 1.5875984110101626 1103 66-67 1.5892603550423274 1103 68-69 1.3346340784322592 1103 70-71 1.1788233825906396 1103 72-73 1.0900199279733478 1103 74-75 1.271290238792698 1103 76-77 1.5398830255563887 1103 78-79 1.5441208879221655 1103 80-81 1.6093055272105765 1103 82-83 1.4691811849343317 1103 84-85 1.5968391293961375 1103 86-87 1.7109231824034445 1103 88-89 1.6207283233576852 1103 90-91 1.4092185115278824 1103 92-93 1.619610772543485 1103 94-95 1.687148116915239 1103 96-97 1.6192272462020796 1103 98-99 1.6131327942208493 1103 100-101 2.028460751902692 1104 1 0.2637141766767108 1104 2 0.14052455063649205 1104 3 0.008855154841526769 1104 4 0.031933769288031044 1104 5 0.13703305278725963 1104 6 0.1136768915162989 1104 7 0.023794984792857576 1104 8 0.24055579649996162 1104 9 0.2974161737969183 1104 10-11 0.20429559143772025 1104 12-13 0.22477613959235043 1104 14-15 0.32610410230290654 1104 16-17 0.3550685998800489 1104 18-19 0.44903077937839697 1104 20-21 0.3397288939694647 1104 22-23 0.5737952636307924 1104 24-25 0.27070925122848166 1104 26-27 0.2528011751715482 1104 28-29 0.34117991102957745 1104 30-31 0.4283938827652136 1104 32-33 0.6170398103470163 1104 34-35 0.5440286550986997 1104 36-37 0.2958921084224926 1104 38-39 0.8346254220713405 1104 40-41 0.8102798141670533 1104 42-43 1.02636836892691 1104 44-45 0.7800701177250389 1104 46-47 0.8196858075204574 1104 48-49 0.8104252164721757 1104 50-51 0.8511164994182465 1104 52-53 0.952082255588941 1104 54-55 1.18170659595949 1104 56-57 1.032313488578417 1104 58-59 1.0704358086614931 1104 60-61 1.3085923243337874 1104 62-63 1.326389623198338 1104 64-65 1.209098558372375 1104 66-67 1.239835886364986 1104 68-69 1.2480260291805294 1104 70-71 1.3630998341084712 1104 72-73 1.2997613891515947 1104 74-75 1.5801737244003213 1104 76-77 1.498204078705026 1104 78-79 1.2325971626053338 1104 80-81 0.8695881351943342 1104 82-83 0.9995828288417208 1104 84-85 1.4624243211307544 1104 86-87 1.6135863451240802 1104 88-89 1.5143532701435518 1104 90-91 1.6039454002202866 1104 92-93 1.7816068756315424 1104 94-95 2.179247864762111 1104 96-97 2.664794918206532 1104 98-99 2.5579456442061144 1104 100-101 2.978251170083915 1105 1 0.39139448856755266 1105 2 0.4015242721625327 1105 3 0.5150744066748132 1105 4 0.33602733653949457 1105 5 0.46226743503918755 1105 6 0.4988295880725033 1105 7 0.31677279970050165 1105 8 0.3311758651902039 1105 9 0.1919673665937225 1105 10-11 0.45771850056238605 1105 12-13 0.17714548889152582 1105 14-15 0.5611593329721742 1105 16-17 0.6971274507108305 1105 18-19 0.6813476827479334 1105 20-21 0.4837115357591131 1105 22-23 0.7002920611802139 1105 24-25 0.5421053340280721 1105 26-27 0.5298479587972764 1105 28-29 0.5162936212310072 1105 30-31 0.38542767093935737 1105 32-33 0.40051470127804123 1105 34-35 0.5516866890725183 1105 36-37 0.7966927210652059 1105 38-39 0.6338921073029411 1105 40-41 0.6757884932722149 1105 42-43 0.897922253638697 1105 44-45 1.0996770053143834 1105 46-47 1.1235821223816913 1105 48-49 1.2919647133625531 1105 50-51 1.3454425924285474 1105 52-53 1.421983861455459 1105 54-55 1.0534113207343836 1105 56-57 1.1997807678877948 1105 58-59 1.339290816551589 1105 60-61 1.5357342532301033 1105 62-63 1.2434972069725845 1105 64-65 1.2665338131760535 1105 66-67 1.3501695909969413 1105 68-69 1.3586962231840616 1105 70-71 1.1360739360299412 1105 72-73 1.4957258373090276 1105 74-75 1.7642102044894408 1105 76-77 1.753286228524022 1105 78-79 1.6622128685368303 1105 80-81 2.013675204720002 1105 82-83 1.6389474440835308 1105 84-85 1.309344863226734 1105 86-87 1.5579495679960722 1105 88-89 1.596421867563027 1105 90-91 1.5253809334979245 1105 92-93 1.6486239553679205 1105 94-95 1.4351886426327134 1105 96-97 1.311225810251461 1105 98-99 1.3990066300039388 1105 100-101 1.9239255411968834 1106 1 -0.04154269309628944 1106 2 -0.026440801812732673 1106 3 -0.12784337164194426 1106 4 -0.0410949045470943 1106 5 -0.08648347449270943 1106 6 -0.11725699936383194 1106 7 -0.23879959903828052 1106 8 -0.32062500796041604 1106 9 -0.32867900740578904 1106 10-11 -0.3042418438470307 1106 12-13 -0.2542909651467937 1106 14-15 -0.26810637161186435 1106 16-17 -0.16738261477546956 1106 18-19 -0.35286587534083935 1106 20-21 -0.2761114086987959 1106 22-23 -0.253564354051413 1106 24-25 -0.2946033732239215 1106 26-27 -0.4349228391653739 1106 28-29 -0.11772590338698308 1106 30-31 -0.26005195554620997 1106 32-33 -0.2777232322654868 1106 34-35 -0.33014995899926447 1106 36-37 -0.4125905678021198 1106 38-39 -0.05518441464710122 1106 40-41 0.12021609453343984 1106 42-43 -0.006561738599970113 1106 44-45 -0.2715029607297552 1106 46-47 -0.045333109245774494 1106 48-49 -0.11853734824308049 1106 50-51 -0.3199557825411361 1106 52-53 -0.25269173883559404 1106 54-55 -0.4788619026466492 1106 56-57 -0.14659929517308257 1106 58-59 0.012814343109916138 1106 60-61 -0.12766315157222863 1106 62-63 0.16198799038271972 1106 64-65 0.036831533282772 1106 66-67 0.05855850683492747 1106 68-69 0.006737697838438805 1106 70-71 0.01878979826617666 1106 72-73 -0.017492692887433492 1106 74-75 0.10384457266794556 1106 76-77 -0.06354920683858367 1106 78-79 0.47968497622540696 1106 80-81 0.5129324203396948 1106 82-83 -0.1666855901148665 1106 84-85 0.11935880938244736 1106 86-87 0.46531374456653296 1106 88-89 0.4748022944366639 1106 90-91 0.5603372759670044 1106 92-93 0.6780624710118666 1106 94-95 0.5751807600229633 1106 96-97 0.8975707047457604 1106 98-99 1.3814223467150732 1106 100-101 2.2273302222543663 1107 1 -0.16339020042766705 1107 2 -0.16822965811771695 1107 3 -0.17139175873872148 1107 4 -0.21756118020691417 1107 5 -0.35847760939006434 1107 6 -0.3179168234107479 1107 7 -0.16907819141365366 1107 8 -0.08492119564370171 1107 9 -0.0478139047664925 1107 10-11 -9.793805039137737E-4 1107 12-13 -0.14149771001483202 1107 14-15 0.021446414311888873 1107 16-17 -0.16078437430626025 1107 18-19 0.059047614395232983 1107 20-21 0.1781407907146928 1107 22-23 0.18903095386648516 1107 24-25 -0.005160557974662083 1107 26-27 -0.242400845030474 1107 28-29 -0.0864575749412353 1107 30-31 -0.26624697020896804 1107 32-33 -0.18820710323322487 1107 34-35 -0.03795787688783747 1107 36-37 -0.5198486323182507 1107 38-39 -0.15903338825413726 1107 40-41 -0.23506249784193045 1107 42-43 -0.6410558734973222 1107 44-45 -0.7665909372693491 1107 46-47 -0.8329797368117511 1107 48-49 -0.5365725388589127 1107 50-51 -0.7636141403123986 1107 52-53 -0.8251624133223956 1107 54-55 -0.97794548035926 1107 56-57 -0.7803969491320188 1107 58-59 -0.755770700878351 1107 60-61 -1.4228610987863064 1107 62-63 -1.184749546274169 1107 64-65 -0.9853458860720679 1107 66-67 -1.28334032014455 1107 68-69 -1.501693387205549 1107 70-71 -1.4192233982147613 1107 72-73 -1.717859261802392 1107 74-75 -1.4798431106165104 1107 76-77 -1.5056679751670252 1107 78-79 -1.5732109182027436 1107 80-81 -1.4341716852321547 1107 82-83 -0.9973307514051903 1107 84-85 -1.1786250615852971 1107 86-87 -1.070280097075699 1107 88-89 -0.788284215827268 1107 90-91 -0.6771993809244954 1107 92-93 -0.5655592298678975 1107 94-95 -0.30520780302688877 1107 96-97 -0.16679879672051712 1107 98-99 -0.6135190022585348 1107 100-101 -0.006064205898127284 1108 1 0.0324521089217491 1108 2 0.00856913550572358 1108 3 0.364627198607252 1108 4 0.22914282668666175 1108 5 -0.02166158181574218 1108 6 0.19987723087619713 1108 7 0.3201936741615299 1108 8 0.35551396161610427 1108 9 0.46773973590563145 1108 10-11 0.2674501757218479 1108 12-13 0.17430366870123493 1108 14-15 0.35231672883149656 1108 16-17 0.4189765046252347 1108 18-19 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1035.0 3 145.0 4 134.0 5 144.0 6 156.0 7 185.0 8 254.0 9 304.0 10 401.0 11 637.0 12 1037.0 13 1625.0 14 1851.0 15 2764.0 16 3804.0 17 5527.0 18 4567.0 19 2554.0 20 2322.0 21 2246.0 22 2294.0 23 2465.0 24 2659.0 25 3012.0 26 3226.0 27 3633.0 28 4148.0 29 5025.0 30 6061.0 31 7344.0 32 9471.0 33 12193.0 34 16806.0 35 25598.0 36 35986.0 37 59877.0 38 61881.0 39 9583.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.151728154825726 23.25033424672041 15.861496101558053 19.736441496895807 2 13.999450729437923 23.678524513680475 41.50797931301474 20.814045443866867 3 17.117608914297815 29.476007093324863 32.64285903340215 20.763524958975175 4 10.47956489807261 20.69083017394814 41.17489430119693 27.654710626782325 5 9.990207624819702 41.49022747422885 35.88243856607868 12.637126334872764 6 24.55891447488086 42.57901507710522 19.18122620949206 13.680844238521855 7 23.445167519807413 38.194912900281736 22.512003491924922 15.847916087985928 8 24.083431995713976 36.78078431631931 21.17812803841549 17.957655649551224 9 25.85109830871702 17.917876537003842 23.213966809315618 33.01705834496352 10-11 21.951812409048816 30.356528641354675 28.839297526127794 18.852361423468714 12-13 23.246557605843183 29.97789128863857 29.0503837192574 17.72516738626085 14-15 20.933235883562958 30.914947516022988 28.59616109637606 19.55565550403799 16-17 17.06155181679137 34.18296060518375 29.66227109255508 19.093216485469803 18-19 16.826039866725093 31.91619885410035 34.43932767273238 16.81843360644217 20-21 17.956476163077156 30.803812055858128 34.41498103561732 16.82473074544739 22-23 18.016969440458162 30.747852367190653 34.09842407729494 17.136754115056245 24-25 17.316791709789737 31.909472967208835 34.242417228506525 16.531318094494907 26-27 17.03662184215356 31.773651916409147 34.642023931759596 16.547702309677696 28-29 16.37003732188597 31.697145380073056 35.085383942494204 16.847433355546773 30-31 17.267105393696884 31.516915241906148 34.62647574324548 16.589503621151493 32-33 16.709934948756022 31.95778779470661 34.52676625536995 16.805511001167417 34-35 17.251419926089437 31.868515257488202 34.19066279752309 16.689402018899276 36-37 17.44825077706501 31.373090404073807 34.08521261821308 17.093446200648106 38-39 16.371178815480334 32.610251992744935 33.988846249869795 17.029722941904936 40-41 16.805904361642153 32.22962729047799 34.23362503533986 16.73084331253999 42-43 16.929563492063494 31.88607804232804 33.7962962962963 17.388062169312168 44-45 17.31926889151587 32.15273587011333 33.240305207205274 17.287690031165525 46-47 17.532976426277973 32.411966416787635 32.77372335184003 17.281333805094356 48-49 17.207881861367998 32.241092230079985 32.98086414751786 17.570161761034157 50-51 17.068052611213453 32.34640455866838 32.48795346083759 18.09758936928058 52-53 17.495439804593765 32.95694150216061 32.12097458362894 17.426644109616678 54-55 17.767342653295746 32.383204226225416 31.83822617581164 18.011226944667204 56-57 17.7315551852458 32.972116006143175 31.435826476816793 17.860502331794233 58-59 17.736221481247593 32.34965920493826 31.755354937684842 18.1587643761293 60-61 17.9498243438727 32.33081215127092 31.90411241992147 17.815251084934904 62-63 17.581867061714025 32.1651659496513 32.34273885539878 17.910228133235893 64-65 17.653895240803315 31.98604048325982 32.266255794422804 18.093808481514056 66-67 17.64595016400381 32.04869193736112 31.72861998730293 18.576737911332135 68-69 17.566669422170786 31.461850045465816 32.00363726543771 18.967843266925684 70-71 17.555498978302992 32.03152381645163 32.22544786038791 18.18752934485746 72-73 17.81216883544149 31.403782281569846 32.20907529349136 18.574973589497297 74-75 17.387027389854495 32.291988711418696 31.774182082105085 18.546801816621723 76-77 17.5138477818747 31.83551315332595 32.08039154086542 18.57024752393393 78-79 17.437236366282 31.746170852011264 31.50426030434761 19.312332477359124 80-81 17.611957573102075 31.455923810153685 31.53909111201131 19.393027504732927 82-83 17.703712399410726 31.774554364917307 31.52439357088413 18.997339664787845 84-85 17.657669275612477 31.229147446771222 31.48574338422266 19.627439893393646 86-87 17.550435326056405 31.11151158830357 31.651990463499075 19.686062622140952 88-89 18.10553640369369 30.529335416614998 31.687706158081397 19.677422021609914 90-91 17.69461864946239 30.400736759086723 32.319683339092705 19.584961252358173 92-93 18.088117613602545 30.392938157624975 32.14947059329264 19.36947363547984 94-95 17.856676371921225 30.426551724708055 32.06950086553675 19.64727103783396 96-97 18.553028136298785 30.57844818037386 31.09309826625909 19.775425417068263 98-99 18.23081475897141 30.833440795151322 30.682994249966107 20.252750195911158 100-101 18.772060051282644 30.07689090286882 31.40177521933994 19.7492738265086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 984.0 1 639.5 2 364.0 3 437.0 4 375.5 5 390.0 6 722.0 7 1309.0 8 1937.0 9 2090.0 10 1639.5 11 1238.5 12 1181.0 13 1195.0 14 1125.5 15 1053.0 16 986.5 17 980.5 18 1034.5 19 1120.0 20 1276.0 21 1473.0 22 1670.0 23 1908.0 24 2196.5 25 2771.5 26 4089.0 27 5411.5 28 5739.5 29 6079.0 30 7154.0 31 8404.5 32 9824.5 33 11328.0 34 13363.5 35 14960.0 36 16394.0 37 17544.0 38 16392.0 39 14572.5 40 13173.5 41 11846.5 42 10818.5 43 10123.5 44 9446.5 45 8900.5 46 8111.5 47 7255.5 48 7129.5 49 6375.0 50 5000.5 51 4004.0 52 3258.5 53 2790.0 54 2423.0 55 2080.0 56 1741.5 57 1375.0 58 1064.0 59 829.5 60 653.0 61 515.5 62 359.5 63 232.5 64 151.0 65 99.5 66 79.0 67 71.5 68 58.0 69 32.0 70 21.0 71 17.5 72 14.5 73 10.0 74 7.5 75 7.0 76 6.0 77 4.0 78 2.0 79 2.0 80 1.0 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25878345370284794 2 0.24326964971811085 3 0.2310566550918285 4 0.22511519824661005 5 0.22445503637491915 6 0.1904566999828358 7 0.17956402909993532 8 0.19078678091868126 9 0.19210710466206313 10-11 0.19672823776389972 12-13 0.19359246887336773 14-15 0.19078678091868126 16-17 0.1876510120281493 18-19 0.18930141670737666 20-21 0.18055427190747173 22-23 0.17494289599809873 24-25 0.17395265319056233 26-27 0.18302987892631273 28-29 0.19359246887336773 30-31 0.18682580968853563 32-33 0.19111686185452673 34-35 0.18600060734892196 36-37 0.17692338161317153 38-39 0.18038923143954896 40-41 0.1767583411452488 42-43 0.18352500033008093 44-45 0.17774858395278523 46-47 0.17923394816408983 48-49 0.1866607692206129 50-51 0.1937575093412905 52-53 0.2018444922695045 54-55 0.1843502026696946 56-57 0.1671859940057302 58-59 0.16553558932650286 60-61 0.17114696523587583 62-63 0.17989411003578076 64-65 0.16834127728118936 66-67 0.17296241038302593 68-69 0.1752729769339442 70-71 0.1709819247679531 72-73 0.1714770461717213 74-75 0.1737876127226396 76-77 0.1851754050093083 78-79 0.18864125483568572 80-81 0.18269979799046726 82-83 0.18170955518293086 84-85 0.1765933006773261 86-87 0.17791362442070796 88-89 0.18104939331123993 90-91 0.18170955518293086 92-93 0.17890386722824436 94-95 0.1790689076961671 96-97 0.17081688430003036 98-99 0.17263232944718043 100-101 0.16982664149249396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302956.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.85745761091591 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07949632944704 41.91989924192565 2 8.669517572192715 8.644802097253779 3 2.3141872806011845 3.4613848273888297 4 1.0714152731781577 2.1367216626467154 5 0.6139194546978006 1.53042315945561 6 0.41763612874678047 1.2493365351467778 7 0.3157773032787103 1.1020697458895345 8 0.2710349291633454 1.081048999347127 9 0.1979056289371569 0.8880364355126357 >10 1.7523038369653605 17.669641776199537 >50 0.18857489627036747 6.463451065300974 >100 0.1022554014987204 8.802330484290492 >500 0.0033199805681402724 1.0249356944361714 >1k 0.002655984454512218 4.0259182752061635 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4474 1.4767821069726297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3426 1.130857286206578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2467 0.8143096687307727 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1794 0.5921651989067719 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 784 0.25878345370284794 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.22412495543907368 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 563 0.18583556688099923 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 555 0.18319491939423546 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 515 0.1699916819604167 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 465 0.15348763516814323 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 437 0.1442453689644701 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 421 0.13896407399094257 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 421 0.13896407399094257 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.13830391211925164 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 406 0.13401285995326054 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 405 0.13368277901741507 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 401 0.13236245527403318 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 397 0.1310421315306513 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 394 0.1300518887231149 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 362 0.11948929877605989 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.11915921784021442 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 351 0.11585840848175973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 328 0.10826654695731393 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 327 0.10793646602146846 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6504046792273466E-4 0.0 22-23 0.0 0.0 0.0 0.0014853642113046117 0.0 24-25 0.0 0.0 0.0 0.0026406474867637546 0.0 26-27 0.0 0.0 0.0 0.003795930762222897 0.0 28-29 0.0 0.0 0.0 0.006601618716909386 0.0 30-31 0.0 0.0 0.0 0.013533318369664243 0.0 32-33 0.0 0.0 0.0 0.022115422701646445 0.0 34-35 0.0 0.0 0.0 0.026571515335560278 0.0 36-37 0.0 0.0 0.0 0.0338332959241606 0.0 38-39 0.0 0.0 0.0 0.046211331018365706 0.0 40-41 0.0 0.0 0.0 0.07756901992368528 0.0 42-43 0.0 0.0 0.0 0.1300518887231149 0.0 44-45 0.0 0.0 0.0 0.18451524313761736 0.0 46-47 0.0 0.0 0.0 0.2952573971137723 0.0 48-49 0.0 0.0 0.0 0.40319386313524075 0.0 50-51 0.0 0.0 0.0 0.4710254954514847 0.0 52-53 0.0 0.0 0.0 0.5619627932769115 0.0 54-55 0.0 0.0 0.0 0.6354058015025285 0.0 56-57 0.0 0.0 0.0 0.8270177847608233 0.0 58-59 0.0 0.0 0.0 1.0750736080486936 0.0 60-61 0.0 0.0 0.0 1.3199936624460316 0.0 62-63 0.0 0.0 0.0 1.5820779255073343 0.0 64-65 0.0 0.0 0.0 1.9313035556318408 0.0 66-67 0.0 0.0 0.0 2.3821941139967517 0.0 68-69 0.0 0.0 0.0 2.912139056496653 0.0 70-71 0.0 0.0 0.0 3.285955716341647 0.0 72-73 0.0 0.0 0.0 3.596891957908079 0.0 74-75 0.0 0.0 0.0 4.040850816620235 0.0 76-77 0.0 0.0 0.0 4.609910350017825 0.0 78-79 0.0 0.0 0.0 5.1061870370614875 0.0 80-81 0.0 0.0 0.0 5.596852348195778 0.0 82-83 0.0 0.0 0.0 6.187697223359168 0.0 84-85 0.0 0.0 0.0 7.098060444420971 0.0 86-87 0.0 0.0 0.0 8.209442955412667 0.0 88-89 0.0 0.0 0.0 9.370007525845338 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 75 1.3559838E-7 44.343143 9 GGTATCA 1870 0.0 36.622 1 CTCTAGG 55 0.002565487 34.57599 2 GTATCAA 2990 0.0 33.56081 1 TTAGGCA 100 1.2927576E-6 33.257355 4 GGACCTG 75 3.0691325E-4 31.673674 6 TAGGCAT 110 2.7164588E-6 30.238966 5 GTGCAGG 80 4.4540784E-4 29.723585 1 CATGGGG 860 0.0 27.62239 4 ATCAACG 3665 0.0 27.227446 3 AGGCTTA 175 4.9185473E-9 27.148865 6 GAAGCGT 70 0.008372913 27.14886 6 AAGCGTG 70 0.008372913 27.14886 7 GGCGAGA 35 0.008386197 27.144371 18-19 TATCAAC 3680 0.0 27.129936 2 TCAACGC 3695 0.0 27.001915 4 CAACGCA 3705 0.0 26.93349 5 GTACATG 2115 0.0 26.758253 1 AACGCAG 3730 0.0 26.748545 6 ATGGGGG 345 0.0 26.16954 5 >>END_MODULE