##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302956 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.205392862329845 33.0 31.0 34.0 31.0 34.0 2 32.40924094587993 34.0 31.0 34.0 31.0 34.0 3 32.44228534836742 34.0 31.0 34.0 31.0 34.0 4 36.078654986202615 37.0 35.0 37.0 35.0 37.0 5 36.023019844465864 37.0 35.0 37.0 35.0 37.0 6 36.08092594304124 37.0 35.0 37.0 35.0 37.0 7 36.04137564530823 37.0 35.0 37.0 35.0 37.0 8 36.026191922259336 37.0 35.0 37.0 35.0 37.0 9 37.6752927817901 39.0 37.0 39.0 35.0 39.0 10-11 37.62704485139756 39.0 37.0 39.0 35.0 39.0 12-13 37.52435337144668 39.0 37.0 39.0 35.0 39.0 14-15 38.67865795693104 40.0 38.0 41.0 34.0 41.0 16-17 38.65635603850064 40.0 38.0 41.0 34.5 41.0 18-19 38.763851846472754 40.0 38.0 41.0 35.0 41.0 20-21 38.75736575608339 40.0 38.0 41.0 35.0 41.0 22-23 38.678705818666735 40.0 38.0 41.0 34.0 41.0 24-25 38.64729861762104 40.0 38.0 41.0 34.0 41.0 26-27 38.43173761206248 40.0 38.0 41.0 34.0 41.0 28-29 38.33712981423045 40.0 38.0 41.0 34.0 41.0 30-31 38.1506703943807 40.0 37.0 41.0 34.0 41.0 32-33 37.85430557572717 40.0 36.0 41.0 33.5 41.0 34-35 37.633750445609266 40.0 36.0 41.0 33.0 41.0 36-37 37.380533806889446 40.0 35.0 41.0 33.0 41.0 38-39 37.17029040520735 40.0 35.0 41.0 33.0 41.0 40-41 36.92084989239362 40.0 35.0 41.0 33.0 41.0 42-43 36.5717364897873 40.0 35.0 41.0 31.5 41.0 44-45 36.0425457822258 39.0 35.0 41.0 30.0 41.0 46-47 35.40360481390037 39.0 35.0 41.0 26.5 41.0 48-49 34.84477943991867 39.0 35.0 41.0 23.0 41.0 50-51 34.54034249197903 39.0 35.0 41.0 19.5 41.0 52-53 34.27825987932241 39.0 35.0 40.0 16.0 41.0 54-55 34.0533196239718 39.0 35.0 40.0 11.0 41.0 56-57 33.79356903312693 38.0 34.0 40.0 6.5 41.0 58-59 33.558435878477404 38.0 34.0 40.0 2.0 41.0 60-61 33.24089801819406 38.0 34.0 40.0 2.0 41.0 62-63 32.85888544871202 38.0 33.0 40.0 2.0 41.0 64-65 32.488381151058235 37.5 33.0 40.0 2.0 41.0 66-67 32.0712512708116 37.0 32.5 39.5 2.0 41.0 68-69 31.590638574578485 36.0 31.5 39.0 2.0 40.0 70-71 31.171046290550443 36.0 31.0 39.0 2.0 40.0 72-73 30.684974055638442 35.0 31.0 38.5 2.0 40.0 74-75 30.180857946368448 35.0 30.0 37.5 2.0 39.5 76-77 28.862887680059153 33.5 28.5 36.0 2.0 38.5 78-79 29.38924629319109 34.5 30.0 36.0 2.0 39.0 80-81 29.15048059784259 34.5 29.0 36.0 2.0 38.5 82-83 28.728671820330344 34.0 29.0 36.0 2.0 37.5 84-85 28.277144205759253 34.0 29.0 35.0 2.0 37.0 86-87 27.923812368792827 34.0 28.5 35.0 2.0 36.5 88-89 27.488714532803442 34.0 27.5 35.0 2.0 36.0 90-91 27.006101546099103 34.0 26.0 35.0 2.0 36.0 92-93 26.580278984406974 33.5 25.5 35.0 2.0 35.0 94-95 26.117279406910576 33.0 24.5 35.0 2.0 35.0 96-97 25.625757535747766 33.0 21.5 35.0 2.0 35.0 98-99 24.915358996025823 32.5 10.0 34.5 2.0 35.0 100-101 22.7192843185149 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27450001793739176 1101 2 0.23752534529872804 1101 3 0.20637427837042566 1101 4 0.1915271086140109 1101 5 0.026601760875699654 1101 6 0.1715873042828875 1101 7 0.15543798210921977 1101 8 0.22759306032605053 1101 9 0.34284914005605316 1101 10-11 0.15415037078628302 1101 12-13 0.22619660841863265 1101 14-15 0.5054389594300019 1101 16-17 0.25045648419486355 1101 18-19 0.14549785589994002 1101 20-21 0.1137802893208999 1101 22-23 0.24299328202683768 1101 24-25 0.12459417832111086 1101 26-27 0.2587908256390108 1101 28-29 0.1856776481277933 1101 30-31 0.35631958761585736 1101 32-33 0.521174470813186 1101 34-35 0.5252219767977593 1101 36-37 0.43760207891985914 1101 38-39 0.7452080969580948 1101 40-41 0.6427179046657372 1101 42-43 0.7972274167216469 1101 44-45 1.3316451012009125 1101 46-47 1.4208488454478214 1101 48-49 1.3296375612489655 1101 50-51 1.2209033307426367 1101 52-53 1.1461503947635947 1101 54-55 1.0255308791442914 1101 56-57 1.2496291204011598 1101 58-59 1.3398625368641675 1101 60-61 1.3562478093449002 1101 62-63 1.2358777068341027 1101 64-65 1.4031172423037077 1101 66-67 1.686882909817495 1101 68-69 1.8729952688599454 1101 70-71 1.523836873678512 1101 72-73 1.6951183064282382 1101 74-75 1.738684924114665 1101 76-77 1.644956505341458 1101 78-79 1.8010079196261337 1101 80-81 1.9458482171336762 1101 82-83 1.8092405876532034 1101 84-85 1.7659874997539475 1101 86-87 1.5099642026862625 1101 88-89 1.980288661237843 1101 90-91 1.9709493606120212 1101 92-93 1.7606437977643274 1101 94-95 1.6225602374840058 1101 96-97 2.064405118295131 1101 98-99 2.0755735254795233 1101 100-101 2.4519753999524276 1102 1 0.16223995633627908 1102 2 0.10558172214535233 1102 3 0.04756886693359519 1102 4 0.16240284199121646 1102 5 -0.12256754963402727 1102 6 -0.06980885053233976 1102 7 0.10655302399077726 1102 8 -0.06342335806790089 1102 9 0.09729311029417431 1102 10-11 0.10794182170335631 1102 12-13 0.05053059135748583 1102 14-15 -0.12078933491842037 1102 16-17 0.02178162804219852 1102 18-19 -0.11388757271115679 1102 20-21 0.23050874564221147 1102 22-23 0.09953158732772494 1102 24-25 0.4272572181664458 1102 26-27 0.23611205128523238 1102 28-29 0.2776448216238876 1102 30-31 0.37849444633950924 1102 32-33 0.15956358623557776 1102 34-35 0.11952618757846523 1102 36-37 0.5747314052669381 1102 38-39 0.36487263312432106 1102 40-41 0.5116407078764027 1102 42-43 0.23586540476136264 1102 44-45 0.4818273849671115 1102 46-47 0.38683454041432697 1102 48-49 0.552522529651398 1102 50-51 0.7157639055149758 1102 52-53 0.6044488053725701 1102 54-55 0.7769307876167559 1102 56-57 0.8636091797397967 1102 58-59 0.8391940773548185 1102 60-61 0.5707718918842204 1102 62-63 0.33211787919381663 1102 64-65 0.5958829678136581 1102 66-67 0.3366692598743164 1102 68-69 0.5065766178112341 1102 70-71 0.27053699760062244 1102 72-73 0.6286502922157595 1102 74-75 1.0084057651395675 1102 76-77 0.6888588691171229 1102 78-79 0.3815881424802505 1102 80-81 0.6840126273210032 1102 82-83 0.7151266307328505 1102 84-85 0.8976997330771717 1102 86-87 1.0622197393307005 1102 88-89 0.8754639261791972 1102 90-91 0.8807870277911753 1102 92-93 1.104149713630985 1102 94-95 1.1587933138889284 1102 96-97 1.2740134524426168 1102 98-99 1.4584774164280674 1102 100-101 1.7626085339477093 1103 1 0.125517824261145 1103 2 0.2613738555976397 1103 3 0.22344949755074595 1103 4 0.18617373133383808 1103 5 0.15464173598189035 1103 6 0.19425758820523242 1103 7 0.17710526306485264 1103 8 0.15137087704236052 1103 9 0.08186897655448178 1103 10-11 0.33595674287621335 1103 12-13 0.4363045822922089 1103 14-15 0.45635827811305063 1103 16-17 0.5427533005667655 1103 18-19 0.5362672075128998 1103 20-21 0.4532605404341652 1103 22-23 0.5071304879989356 1103 24-25 0.2221120480878298 1103 26-27 0.5332571495122949 1103 28-29 0.3654055080457397 1103 30-31 0.7183523000138194 1103 32-33 0.6325833274502273 1103 34-35 0.7837369281883682 1103 36-37 0.9167837251740067 1103 38-39 0.9158273606572322 1103 40-41 1.0373905087877233 1103 42-43 1.1502572711653727 1103 44-45 1.125473611862347 1103 46-47 1.3347990457463084 1103 48-49 1.480991313144699 1103 50-51 1.6613273713944707 1103 52-53 1.660325364401615 1103 54-55 1.8849805567936428 1103 56-57 1.931607694807667 1103 58-59 1.7777669857261245 1103 60-61 1.7699623643680695 1103 62-63 1.9531637983248942 1103 64-65 2.4100778412052364 1103 66-67 2.490575290016043 1103 68-69 2.5348352355070745 1103 70-71 2.1147748022961252 1103 72-73 2.127883875632417 1103 74-75 2.430228279824231 1103 76-77 2.26045894336373 1103 78-79 2.334684046342872 1103 80-81 2.2360181838412316 1103 82-83 2.1454036783524693 1103 84-85 1.925398507409401 1103 86-87 2.483272843617243 1103 88-89 2.4186946115831525 1103 90-91 2.4413728574670657 1103 92-93 2.453791354001975 1103 94-95 2.7365007702840174 1103 96-97 2.7917907245983393 1103 98-99 2.711622418853061 1103 100-101 2.9401387565078956 1104 1 0.25836486228059385 1104 2 0.1309419205679987 1104 3 0.14645273951950344 1104 4 0.17897754310316571 1104 5 0.17894012809637871 1104 6 0.17770389923774843 1104 7 0.040811586541288136 1104 8 0.15184571085053022 1104 9 -0.009888563478916979 1104 10-11 0.17081281911160318 1104 12-13 0.21871608309452029 1104 14-15 0.19077881713129585 1104 16-17 0.165456906028993 1104 18-19 0.17473447672729492 1104 20-21 0.0507160861655791 1104 22-23 0.07220318669232739 1104 24-25 0.05656287822831274 1104 26-27 0.1931042202789044 1104 28-29 0.2568445819560736 1104 30-31 0.30138272212470696 1104 32-33 0.21196653470699545 1104 34-35 0.12527751829217237 1104 36-37 0.00430460148799483 1104 38-39 0.06287029218292872 1104 40-41 0.19536280469105094 1104 42-43 0.3007673081696609 1104 44-45 0.30075278139438666 1104 46-47 0.04265345187657488 1104 48-49 0.06661694762090775 1104 50-51 0.07918112258155219 1104 52-53 0.18047714748443155 1104 54-55 0.0054164214823728685 1104 56-57 0.2139700748711988 1104 58-59 0.43712694249987294 1104 60-61 0.1765183456366941 1104 62-63 0.3852175364131156 1104 64-65 0.36611887339569904 1104 66-67 0.619856981079348 1104 68-69 0.7581790479313497 1104 70-71 0.7563694252125828 1104 72-73 0.6439709175815338 1104 74-75 1.0331075737954798 1104 76-77 0.7602297132494442 1104 78-79 0.8332922920775552 1104 80-81 1.1173097317994447 1104 82-83 0.6530951673657945 1104 84-85 0.9534259822759559 1104 86-87 0.8805982193908974 1104 88-89 0.4805171547275364 1104 90-91 0.49473025996239883 1104 92-93 0.058553609626301295 1104 94-95 -0.09302161239928353 1104 96-97 0.3757503473510653 1104 98-99 0.4513152504999134 1104 100-101 1.1292863479543378 1105 1 0.20635984974926913 1105 2 0.3014608103851373 1105 3 0.17506593918533042 1105 4 0.16150330194471252 1105 5 0.12264197530699761 1105 6 0.09109849684287497 1105 7 0.3147371411685995 1105 8 0.2005322419932014 1105 9 0.21706771610893583 1105 10-11 0.15403476285985818 1105 12-13 0.08133559483684394 1105 14-15 0.11306137537876282 1105 16-17 0.23862593972434354 1105 18-19 0.2616067500030752 1105 20-21 0.23882524795894966 1105 22-23 0.05761811289672636 1105 24-25 0.058059675777741404 1105 26-27 0.08828197949175376 1105 28-29 0.0956661729706525 1105 30-31 0.04996714614772202 1105 32-33 -0.08174227435269188 1105 34-35 -0.12081224314845684 1105 36-37 -0.23340773490856037 1105 38-39 -0.024500913609330155 1105 40-41 0.019715352727807556 1105 42-43 0.2222492537744145 1105 44-45 0.0526788929696238 1105 46-47 -0.261648970846899 1105 48-49 -0.10615294006125708 1105 50-51 -0.17817523126603163 1105 52-53 -0.222300630293347 1105 54-55 0.2299128477331891 1105 56-57 0.28739437636567544 1105 58-59 -0.0837934139468004 1105 60-61 -0.22731531258292392 1105 62-63 -0.5218765877862737 1105 64-65 -0.5008982063406862 1105 66-67 -0.5682637837958033 1105 68-69 -0.27929890621327047 1105 70-71 -0.0028086124742259244 1105 72-73 -0.07128945557357724 1105 74-75 0.05293724055496796 1105 76-77 -0.3462401651502631 1105 78-79 -0.02473239928047022 1105 80-81 -0.19905989597369 1105 82-83 -0.3695192390205406 1105 84-85 -0.06229619353394256 1105 86-87 -0.09277270792740211 1105 88-89 0.13974624597416963 1105 90-91 0.0070875420565243985 1105 92-93 0.5144502957861441 1105 94-95 0.5254110433140724 1105 96-97 0.6394303807679407 1105 98-99 0.81300651569995 1105 100-101 1.5970761929371697 1106 1 -0.4970114818054263 1106 2 -0.43974704797043174 1106 3 -0.5264863285808552 1106 4 -0.40495316179527663 1106 5 -0.5987380877538797 1106 6 -0.5791658737610561 1106 7 -0.4238937101532514 1106 8 -0.5065075348683123 1106 9 -0.9169972851037684 1106 10-11 -0.8203012788573361 1106 12-13 -1.1562341359417232 1106 14-15 -0.4239414138523827 1106 16-17 -0.657945125034324 1106 18-19 -0.974583524069196 1106 20-21 -0.9262851085775523 1106 22-23 -0.6574861800938194 1106 24-25 -1.2889419007442129 1106 26-27 -1.4242544017840189 1106 28-29 -1.5039578871797232 1106 30-31 -1.5003148087394962 1106 32-33 -1.2530374477701045 1106 34-35 -1.5326028082709442 1106 36-37 -1.2140347055609908 1106 38-39 -1.2156542000512474 1106 40-41 -1.8297268010437122 1106 42-43 -2.417970246589398 1106 44-45 -2.0094572942726785 1106 46-47 -1.5922031789477984 1106 48-49 -1.2882715430202651 1106 50-51 -1.503529948704788 1106 52-53 -1.8130264367449627 1106 54-55 -1.8436228059170645 1106 56-57 -2.0625631788125958 1106 58-59 -2.2785241925061044 1106 60-61 -2.30254875910245 1106 62-63 -2.3857651398114967 1106 64-65 -2.395256076823344 1106 66-67 -2.2867788213897917 1106 68-69 -1.605047754139541 1106 70-71 -2.182350208399523 1106 72-73 -2.3315368482488132 1106 74-75 -2.458280319474138 1106 76-77 -1.9706792871488048 1106 78-79 -1.7545711089578901 1106 80-81 -1.8810723868783725 1106 82-83 -1.555412399340696 1106 84-85 -2.1085762080864505 1106 86-87 -2.1946705978134062 1106 88-89 -2.322599133603809 1106 90-91 -1.8794483541355689 1106 92-93 -1.9884147296807484 1106 94-95 -2.2396265506708914 1106 96-97 -2.3948589716453483 1106 98-99 -3.0258850680982547 1106 100-101 -2.6575017842639035 1107 1 0.24169819561392814 1107 2 0.11567817197092012 1107 3 0.17776587083263706 1107 4 0.16146912559474913 1107 5 0.01973698555990211 1107 6 0.17787224940610002 1107 7 0.1005197795828181 1107 8 -0.029748004076516565 1107 9 0.28388248029212093 1107 10-11 0.3001899234887091 1107 12-13 0.33482158253335115 1107 14-15 0.28929172397753433 1107 16-17 0.03607980165188707 1107 18-19 0.15719800085748403 1107 20-21 0.5369113723608692 1107 22-23 0.3955196850088285 1107 24-25 0.29907129573673075 1107 26-27 0.30166202050337176 1107 28-29 -0.01986697808881388 1107 30-31 -0.02872389964858968 1107 32-33 -0.1885946325208323 1107 34-35 0.15350422985222423 1107 36-37 -0.21623411905073198 1107 38-39 -0.32239906808643326 1107 40-41 0.12364563297388287 1107 42-43 -0.10543358969790262 1107 44-45 0.04387848285342244 1107 46-47 0.03331001753313956 1107 48-49 0.43914781181843665 1107 50-51 0.3335301685972638 1107 52-53 0.4049720969793782 1107 54-55 0.45897983337912507 1107 56-57 0.26610251033696386 1107 58-59 -0.20007844470551817 1107 60-61 -0.4488464130613963 1107 62-63 -0.35552320432762485 1107 64-65 -0.261715021104866 1107 66-67 0.11663026951930178 1107 68-69 -0.24423397114833634 1107 70-71 -0.35639712922063893 1107 72-73 -0.2662142676036545 1107 74-75 -0.48291375055918095 1107 76-77 -0.18376579741273602 1107 78-79 -0.41564149018956087 1107 80-81 -0.5638069909839452 1107 82-83 -0.5479710503670887 1107 84-85 -0.28097356879026236 1107 86-87 -0.45273511394243826 1107 88-89 -0.7131416555979442 1107 90-91 -0.6736199083877672 1107 92-93 -0.9778942018215098 1107 94-95 -0.40326291430725547 1107 96-97 -0.3555627839620641 1107 98-99 -0.14054782469649751 1107 100-101 0.3977768081114661 1108 1 0.22084511504520776 1108 2 0.2977548630523472 1108 3 0.22057069633888204 1108 4 0.1318913573913889 1108 5 0.17197739917472177 1108 6 0.19762666560601616 1108 7 0.29948574252982496 1108 8 0.09231145305403032 1108 9 -0.09537215133217813 1108 10-11 0.06558199587130531 1108 12-13 0.3884837970960433 1108 14-15 -0.051951274729923114 1108 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0.6123473671113508 2316 98-99 0.3466109349112436 2316 100-101 0.8801051940112821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 0.0 5 14.0 6 20.0 7 42.0 8 120.0 9 221.0 10 370.0 11 726.0 12 1141.0 13 1786.0 14 2336.0 15 3343.0 16 4898.0 17 6867.0 18 5724.0 19 3096.0 20 2252.0 21 2169.0 22 2172.0 23 2414.0 24 2557.0 25 2935.0 26 3263.0 27 3741.0 28 4239.0 29 5018.0 30 5996.0 31 7432.0 32 9140.0 33 11956.0 34 16375.0 35 24059.0 36 34662.0 37 62201.0 38 63302.0 39 6367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.571387055120915 23.16921339334956 17.019751855142054 19.23964769638747 2 14.370733703904198 23.44366838748861 41.78329526399873 20.402302644608458 3 17.16090785460595 28.552661112504786 33.97853153593261 20.307899496956654 4 10.601209416548937 20.9056760717728 41.257476333196905 27.235638178481363 5 9.973988076768183 40.8014181119569 36.4011777987575 12.823416012517413 6 24.874239163442876 42.130870489444014 18.802070267629624 14.19282007948349 7 23.13339230779387 38.26925362098786 22.539906785143717 16.057447286074545 8 24.17017652728449 36.03460568531404 21.47968681920807 18.3155309681934 9 25.732779677576943 17.33122961750221 23.455881382114896 33.48010932280595 10-11 22.033067508153 29.633676177398698 29.26580097439892 19.06745534004938 12-13 23.516799836268238 29.335054277409807 29.544175391049883 17.60397049527208 14-15 20.910957366746327 29.631200570379857 30.214453584018802 19.243388478855014 16-17 16.883474828027833 32.86368317511454 31.22169555975125 19.03114643710638 18-19 16.55685974200874 29.795910957366743 36.82960561929785 16.81762368132666 20-21 17.228494707951196 28.87463298944231 37.289923074257025 16.60694922834947 22-23 17.260490110644593 28.708508278539174 37.117266101560645 16.91373550925559 24-25 17.00378932914351 29.59258110088594 37.18378246346004 16.219847106510514 26-27 16.812757157498808 29.22348956846228 37.896329940601056 16.067423333437862 28-29 16.283023134142375 29.493267051878114 37.91345450824475 16.310255305734767 30-31 17.0386128678752 29.484479594396547 37.29006852480229 16.18683901292597 32-33 16.37812091524842 29.577397377837045 37.50478617356976 16.539695533344776 34-35 16.933812170744268 29.59126077714255 36.94430874450415 16.53061830760903 36-37 17.45066907847396 29.125213728816917 36.699465925520045 16.724651267189078 38-39 16.244108055295158 30.38609567065845 36.67941879348816 16.690377480558233 40-41 16.661412852425034 29.992952737127194 36.73158840683997 16.6140460036078 42-43 16.900311012209375 29.549626587251804 36.4008095561968 17.149252844342023 44-45 17.325453201124915 29.67312084923223 35.84233354018405 17.159092409458797 46-47 17.482406686119436 30.124011407597145 35.23399437542085 17.159587530862566 48-49 17.209594792643156 30.52208901622678 34.69216651923052 17.57614967189955 50-51 17.130045287104398 30.424550099684446 34.454013124018005 17.991391489193152 52-53 17.683785242387085 31.09763645155807 33.837477781390334 17.381100524664514 54-55 17.860679438598346 30.10140086349173 33.84550891878689 18.19241077912304 56-57 18.1209482565125 30.65065554073859 33.361280185901585 17.86711601684733 58-59 17.92686066623536 30.688779888828744 33.29955505089848 18.08480439403742 60-61 17.930161475593813 30.69670183128903 33.59415228614056 17.77898440697659 62-63 17.6999300228416 30.440228944137104 33.68806031238859 18.1717807206327 64-65 17.464087194180014 30.307041286523457 33.925718586197334 18.303152933099195 66-67 17.576974874239163 30.71518636369638 33.05413987509737 18.653698886967085 68-69 17.746801515731658 30.00105625899471 33.12015606226647 19.13198616300717 70-71 17.85127213192675 30.36827130012279 33.56659052799746 18.213866039952997 72-73 17.801429910614083 29.54636976986757 34.00130712050595 18.650893199012398 74-75 17.54957815656399 30.75100014523561 32.813510872866026 18.885910825334374 76-77 17.33733611481535 30.06393667727327 33.46707112584005 19.131656082071324 78-79 17.291619905200754 30.145796749362948 32.795686502330376 19.76689684310593 80-81 17.319016622875928 29.818356461004235 32.75822231611191 20.10440460000792 82-83 17.107628017976236 30.186611565064837 33.01491473170152 19.690845685257404 84-85 17.086969724976562 29.613046118908354 32.65457690225643 20.645407253858647 86-87 16.597789778053578 29.38941628487305 32.79964747356052 21.213146463512853 88-89 17.088455089187867 29.004541913677233 32.91633108438189 20.990671912753005 90-91 16.48080656023452 28.70845382878422 33.67286640520805 21.137873205773218 92-93 16.56678654481897 28.300871606042648 33.84657026900789 21.285771580130486 94-95 16.135511427402 28.212842789051873 34.01566564121523 21.635980142330897 96-97 16.867465902639328 28.495722151071444 32.8357913360356 21.801020610253634 98-99 16.3028857659204 28.843465559782473 32.41019631790462 22.443452356392505 100-101 15.64782046033779 27.896754129966855 33.506213107610236 22.94921230208512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1042.0 1 674.0 2 379.5 3 468.0 4 392.0 5 376.5 6 686.0 7 1317.5 8 1862.0 9 2029.0 10 1734.5 11 1305.0 12 1148.5 13 1127.5 14 1097.5 15 1031.0 16 1028.5 17 1095.0 18 1166.5 19 1196.5 20 1325.5 21 1524.0 22 1739.0 23 1975.0 24 2210.5 25 2841.0 26 4032.5 27 5205.0 28 5569.5 29 6229.5 30 7457.0 31 8179.0 32 9353.0 33 10835.5 34 12866.5 35 14730.0 36 16144.5 37 17032.0 38 16191.5 39 14824.5 40 13374.0 41 12019.0 42 10776.0 43 10072.5 44 9720.0 45 9162.0 46 8279.5 47 7428.0 48 7355.5 49 6626.5 50 5189.0 51 4144.0 52 3319.0 53 2753.5 54 2420.5 55 2078.0 56 1721.5 57 1399.0 58 1095.0 59 823.0 60 644.0 61 509.5 62 346.0 63 221.0 64 148.5 65 103.5 66 81.5 67 69.5 68 46.0 69 32.5 70 26.5 71 17.0 72 12.5 73 11.5 74 10.0 75 6.5 76 4.0 77 3.0 78 1.5 79 1.5 80 1.5 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04918205944097493 2 0.0 3 0.0 4 0.0 5 0.005941456845218448 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00709674012067759 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.951214037682039E-4 22-23 0.0013203237433818773 24-25 0.0 26-27 0.001485364211304612 28-29 0.0018154451471500811 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.601618716909387E-4 38-39 0.0 40-41 4.951214037682039E-4 42-43 0.024425989252564728 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.6504046792273466E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6504046792273466E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.013203237433818772 92-93 0.0031357688905319582 94-95 0.0 96-97 0.0 98-99 0.0011552832754591425 100-101 0.015843884920582526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302956.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.31415770267594 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39008261885671 40.28921602486619 2 8.938544916353703 8.637165374423303 3 2.5317165412299216 3.669532566943671 4 1.1675287233276421 2.256326674450224 5 0.6771438401765716 1.6357817140843236 6 0.4200959770408008 1.2177949971005388 7 0.3474116954427098 1.1749432408961173 8 0.2779251436255376 1.0742175374930467 9 0.22024096517205288 0.9576681051520909 >10 1.7233367078113768 17.088791754735002 >50 0.19757889425415331 6.643651904859772 >100 0.09878944712707666 8.27208244809346 >500 0.006174340445442291 1.9201095332694402 >1k 0.0027441513090854627 3.4603734726968676 >5k 6.860378272713657E-4 1.7023446509359301 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5136 1.6952956865023305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4242 1.4002033298564809 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3368 1.1117125919275406 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1742 0.5750009902428075 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1088 0.35912805819987065 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 887 0.29278179009493127 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 762 0.2515216731142476 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 661 0.21818349859385522 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 639 0.2109217180052549 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 610 0.20134937086573626 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 574 0.1894664571752994 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.1848453240734628 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 559 0.18451524313761733 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 541 0.1785737862923989 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 500 0.16504046792273466 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 497 0.16405022511519826 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 488 0.16107949669258903 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 464 0.15315755423229777 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 421 0.13896407399094257 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 359 0.11849905596852349 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 320 0.10562589947055018 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 320 0.10562589947055018 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 313 0.10331533291963188 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 309 0.10199500917625001 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 308 0.10166492824040456 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 305 0.10067468543286813 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0011552832754591425 0.0 24-25 0.0 0.0 0.0 0.002310566550918285 0.0 26-27 0.0 0.0 0.0 0.0034658498263774274 0.0 28-29 0.0 0.0 0.0 0.006271537781063917 0.0 30-31 0.0 0.0 0.0 0.013203237433818772 0.0 32-33 0.0 0.0 0.0 0.02162030129787824 0.0 34-35 0.0 0.0 0.0 0.026076393931792077 0.0 36-37 0.0 0.0 0.0 0.03366825545623787 0.0 38-39 0.0 0.0 0.0 0.04604629055044297 0.0 40-41 0.0 0.0 0.0 0.07888934366706717 0.0 42-43 0.0 0.0 0.0 0.1310421315306513 0.0 44-45 0.0 0.0 0.0 0.1861656478168447 0.0 46-47 0.0 0.0 0.0 0.29938340881184067 0.0 48-49 0.0 0.0 0.0 0.4089702795125365 0.0 50-51 0.0 0.0 0.0 0.47944255931554414 0.0 52-53 0.0 0.0 0.0 0.5708749785447391 0.0 54-55 0.0 0.0 0.0 0.6441529463024334 0.0 56-57 0.0 0.0 0.0 0.8369202128361874 0.0 58-59 0.0 0.0 0.0 1.085471157527826 0.0 60-61 0.0 0.0 0.0 1.3331968998798505 0.0 62-63 0.0 0.0 0.0 1.5952811629411532 0.0 64-65 0.0 0.0 0.0 1.9440116716618914 0.0 66-67 0.0 0.0 0.0 2.396057513302262 0.0 68-69 0.0 0.0 0.0 2.9276528604813903 0.0 70-71 0.0 0.0 0.0 3.303284965473534 0.0 72-73 0.0 0.0 0.0 3.61504640937958 0.0 74-75 0.0 0.0 0.0 4.063791441661495 0.0 76-77 0.0 0.0 0.0 4.638297310500535 0.0 78-79 0.0 0.0 0.0 5.1380398473705755 0.0 80-81 0.0 0.0 0.0 5.632831170202934 0.0 82-83 0.0 0.0 0.0 6.227306935660623 0.0 84-85 0.0 0.0 0.0 7.144106734971414 0.0 86-87 0.0 0.0 0.0 8.264731512166783 0.0 88-89 0.0 0.0 0.0 9.431402579912595 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 135 0.0 52.777317 9 TTAGGCA 155 0.0 45.967342 4 GGTATCA 1755 0.0 44.96549 1 GCATTCC 180 0.0 44.86072 8 TAGGCAT 180 0.0 44.86072 5 CCTTTAG 170 0.0 41.94602 1 GGCATTC 190 0.0 37.499676 7 GTATCAA 2900 0.0 36.39178 1 TAGGACA 135 3.074092E-10 35.18488 4 CTTTAGG 215 0.0 33.139244 2 AGGCATT 230 0.0 33.04319 6 GGATACC 60 0.003946568 31.666391 6 ACTATAC 75 3.0732955E-4 31.66639 3 CAGTACC 65 0.0058376184 29.230515 4 TAGTGAC 65 0.0058376184 29.230515 7 CATATAG 100 4.9415303E-5 28.499754 3 ACGCATT 85 6.396532E-4 27.940933 6 CGCATTC 85 6.396532E-4 27.940933 7 TACGCAT 85 6.396532E-4 27.940933 5 TATCAAC 3805 0.0 27.58846 2 >>END_MODULE