FastQCFastQC Report
Thu 26 May 2016
SRR937644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257863
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40111.5554771332063926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30461.1812474065686043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.8787612026541226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16590.6433648875565707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.28968871067194596No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.23462070944648902No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4850.1880843703827226No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4250.1648162008508394No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA4070.15783574999127442No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.15589673586361752No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA3820.14814067935298977No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.147365073701927No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.14581386239980143No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT3660.14193583414448757No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT3620.14038462284236203No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3420.13262856633173428No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC3410.1322407635062029No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA3410.1322407635062029No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3390.13146515785514012No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.12409690417004378No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2980.11556524200835327No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA2960.11478963635729049No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.11323842505516495No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC2630.10199214311475474No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG200.002065295171.310315
ATCACGC200.002070085671.268683
TCACGCA250.005003931557.037144
TATCACG250.00500778957.0260432
GGTATCA17850.043.944731
CATTCCG901.1856173E-842.2415129
GGCATTC1208.54925E-1139.593717
GTCGGGA250.001612135238.0136612-13
GTCCTAC550.00256524734.5746961
GTACAGG702.0387518E-433.957291
GTATCAA27700.033.1236841
TAGGCAT1151.0234362E-733.071455
TTAGGCA1351.1819793E-831.68734
TACAGGG902.39823E-531.6811352
ATCGTGA902.401424E-531.674977
TATCGTG902.401424E-531.674976
ATGGGAT3350.029.8013255
GTATAGG1503.288187E-828.5241261
TGTATCG1004.91647E-528.518574
TATCAAC32850.027.9199052
Produced by FastQC (version 0.11.5)