##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937644_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 257863 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9924843812412 33.0 31.0 34.0 31.0 34.0 2 32.077133981998195 33.0 31.0 34.0 30.0 34.0 3 32.085359279927715 34.0 31.0 34.0 30.0 34.0 4 35.71214559669282 37.0 35.0 37.0 35.0 37.0 5 35.73655778456002 37.0 35.0 37.0 35.0 37.0 6 35.84007011475086 37.0 35.0 37.0 35.0 37.0 7 35.78976433222292 37.0 35.0 37.0 35.0 37.0 8 35.80184826826648 37.0 35.0 37.0 35.0 37.0 9 37.37046028317362 39.0 37.0 39.0 35.0 39.0 10-11 37.34224956663034 39.0 37.0 39.0 35.0 39.0 12-13 37.26508068237786 39.0 37.0 39.0 34.5 39.0 14-15 38.39017617882364 40.0 38.0 41.0 34.0 41.0 16-17 38.35575480002947 40.0 38.0 41.0 34.0 41.0 18-19 38.41151696831263 40.0 38.0 41.0 34.0 41.0 20-21 38.48820497706146 40.0 38.0 41.0 34.0 41.0 22-23 38.446124492463056 40.0 38.0 41.0 34.0 41.0 24-25 38.37708783346196 40.0 38.0 41.0 34.0 41.0 26-27 38.25473410299267 40.0 38.0 41.0 34.0 41.0 28-29 38.105969061090576 40.0 38.0 41.0 34.0 41.0 30-31 37.78991557532488 40.0 37.0 41.0 34.0 41.0 32-33 37.50980753345769 40.0 36.0 41.0 33.0 41.0 34-35 37.39905298550005 40.0 36.0 41.0 33.0 41.0 36-37 37.189484338582886 40.0 35.0 41.0 33.0 41.0 38-39 36.97762183795271 40.0 35.0 41.0 33.0 41.0 40-41 36.73270496348836 39.5 35.0 41.0 32.0 41.0 42-43 36.38615272450875 39.0 35.0 41.0 31.0 41.0 44-45 35.96790543815902 39.0 35.0 41.0 30.0 41.0 46-47 35.40296785502379 39.0 35.0 41.0 27.0 41.0 48-49 34.91530386290394 39.0 35.0 41.0 23.5 41.0 50-51 33.99562946215626 38.0 34.0 39.5 21.5 40.5 52-53 34.066114564710716 38.0 34.5 40.0 20.5 40.5 54-55 34.32398793157607 39.0 35.0 40.0 18.5 41.0 56-57 34.11184039586912 39.0 35.0 40.0 12.0 41.0 58-59 33.87239735828715 38.0 34.5 40.0 7.5 41.0 60-61 33.576486351279556 38.0 34.0 40.0 2.0 41.0 62-63 33.247466678042215 38.0 34.0 40.0 2.0 41.0 64-65 32.86100565028717 38.0 33.0 40.0 2.0 41.0 66-67 32.36990378611898 37.0 33.0 40.0 2.0 41.0 68-69 31.911675967471098 36.5 32.0 39.0 2.0 41.0 70-71 31.45963942093282 36.0 31.5 39.0 2.0 40.0 72-73 30.99976925731881 35.5 31.0 38.5 2.0 40.0 74-75 30.447043585159562 35.0 31.0 38.0 2.0 40.0 76-77 29.91601160306054 35.0 30.0 37.0 2.0 39.0 78-79 29.408666617544974 35.0 29.0 36.5 2.0 39.0 80-81 28.892504934790956 34.0 29.0 36.0 2.0 38.5 82-83 28.367229885636945 34.0 28.5 36.0 2.0 37.0 84-85 27.8280986415267 34.0 27.0 35.0 2.0 37.0 86-87 27.304146000007755 34.0 26.0 35.0 2.0 36.0 88-89 26.76684518523402 33.5 25.5 35.0 2.0 36.0 90-91 26.254059326076252 33.0 24.5 35.0 2.0 35.5 92-93 25.667086786394325 33.0 21.5 35.0 2.0 35.0 94-95 25.011108611937345 32.0 17.5 35.0 2.0 35.0 96-97 24.256682812190967 32.0 4.5 34.0 2.0 35.0 98-99 23.253840217479826 31.0 2.0 34.0 2.0 35.0 100-101 20.294074760628703 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18520053739286269 1101 2 0.29350479704785215 1101 3 0.10225067267854371 1101 4 0.3078097840315266 1101 5 0.35468044900942886 1101 6 0.46063308232999844 1101 7 0.36879137513662386 1101 8 0.3951902949474899 1101 9 0.7173980041424173 1101 10-11 0.5627089370418332 1101 12-13 0.4626842127390063 1101 14-15 0.7053468950997157 1101 16-17 0.7284507173757859 1101 18-19 0.7927741107980424 1101 20-21 0.723629142493202 1101 22-23 0.6649671459508113 1101 24-25 0.7538506888222329 1101 26-27 1.0886443171332374 1101 28-29 0.8076626254654826 1101 30-31 0.8312888685171131 1101 32-33 0.8617159343057637 1101 34-35 1.0434724715968642 1101 36-37 1.0938412398574684 1101 38-39 1.3128387157805932 1101 40-41 1.267410011513796 1101 42-43 1.401586753888516 1101 44-45 1.6508375937460684 1101 46-47 1.8997996905197354 1101 48-49 2.1382387565571506 1101 50-51 2.0245218300784416 1101 52-53 2.263210143368191 1101 54-55 2.2612712168109255 1101 56-57 2.1601447878717153 1101 58-59 2.5362315487054516 1101 60-61 2.5345725926896847 1101 62-63 2.449503022102512 1101 64-65 2.470123960887271 1101 66-67 2.878528052772424 1101 68-69 2.808575240679133 1101 70-71 2.715836031366951 1101 72-73 2.7047571845780887 1101 74-75 2.950441789340875 1101 76-77 2.6512392732400336 1101 78-79 2.6676074300189008 1101 80-81 2.9027755627695093 1101 82-83 2.9031545972508077 1101 84-85 2.7877802367947098 1101 86-87 2.9542487584001123 1101 88-89 2.872437664675317 1101 90-91 3.120055475845483 1101 92-93 3.2250559106427126 1101 94-95 3.398741290279659 1101 96-97 3.265475786071672 1101 98-99 3.1609888321302613 1101 100-101 3.318240547349731 1102 1 0.25169085238849576 1102 2 0.22411830013759015 1102 3 0.3183244823047673 1102 4 0.37433594286272864 1102 5 0.18913927877989067 1102 6 0.1466554202083259 1102 7 0.26825658510981043 1102 8 0.23638059703933578 1102 9 0.3260119947083169 1102 10-11 0.39037894435397646 1102 12-13 0.30270600579103757 1102 14-15 0.49046668519822134 1102 16-17 0.7031664757596516 1102 18-19 0.46378505033666073 1102 20-21 0.39988761956031027 1102 22-23 0.3836217117385843 1102 24-25 0.26540674141229914 1102 26-27 0.5224443372057905 1102 28-29 0.44244620038627147 1102 30-31 0.4666136853551208 1102 32-33 0.8063766365581913 1102 34-35 0.6837186470595995 1102 36-37 0.9392646989309767 1102 38-39 0.8259253001498195 1102 40-41 1.0699033087165404 1102 42-43 1.3585383073603552 1102 44-45 1.6568020079861085 1102 46-47 1.467734512089116 1102 48-49 1.6786849406224107 1102 50-51 1.356794185878691 1102 52-53 1.5585095827706041 1102 54-55 1.6352371221545283 1102 56-57 1.4273713352501005 1102 58-59 1.3637295056068268 1102 60-61 1.3475502117987475 1102 62-63 1.47335351029556 1102 64-65 1.5816843147376218 1102 66-67 1.8345620040534811 1102 68-69 1.7854810574167743 1102 70-71 1.6807771757889363 1102 72-73 1.655833933398828 1102 74-75 1.3827650433876464 1102 76-77 1.4004542932408945 1102 78-79 1.7841214019460132 1102 80-81 1.5917693546171883 1102 82-83 1.7226808851198179 1102 84-85 2.179130402393227 1102 86-87 1.757924902176903 1102 88-89 1.8866891737007982 1102 90-91 2.1363787585673215 1102 92-93 2.1070866789911697 1102 94-95 2.5402885969738556 1102 96-97 2.9045319461932557 1102 98-99 3.086946220978529 1102 100-101 3.357037914978445 1103 1 0.3585563194581489 1103 2 0.3304390283304599 1103 3 0.27111670835382995 1103 4 0.40056248394657956 1103 5 0.32658919794766916 1103 6 0.44614758299739066 1103 7 0.26687716569602316 1103 8 0.471303630707709 1103 9 0.43064578960534305 1103 10-11 0.4301097379155152 1103 12-13 0.6560921729380098 1103 14-15 0.7828860287000126 1103 16-17 0.6846453539490156 1103 18-19 0.660774050125795 1103 20-21 0.5812768794183327 1103 22-23 0.5776673643709103 1103 24-25 0.5908926133459502 1103 26-27 0.47398893550479926 1103 28-29 0.3628440354885427 1103 30-31 0.4968027624620319 1103 32-33 0.7252412953056435 1103 34-35 0.9613905033891186 1103 36-37 0.8257305736761751 1103 38-39 0.7944095202946073 1103 40-41 0.9132205927539374 1103 42-43 1.009242074844714 1103 44-45 1.1000700898023723 1103 46-47 1.2065585790041382 1103 48-49 0.8491354923901824 1103 50-51 0.7988893218876854 1103 52-53 1.1202056536218024 1103 54-55 0.7942920880644166 1103 56-57 0.8640717991567541 1103 58-59 0.7243686073148439 1103 60-61 1.050627986452568 1103 62-63 1.4537121593431408 1103 64-65 1.3224325399432146 1103 66-67 1.2465734962865582 1103 68-69 1.264921557873656 1103 70-71 1.2537106825015236 1103 72-73 1.2851479138585198 1103 74-75 1.3279748557163167 1103 76-77 1.5441906747689806 1103 78-79 1.6009968851821093 1103 80-81 1.6852579678174386 1103 82-83 1.7244293211920763 1103 84-85 1.613976996896639 1103 86-87 2.0560112872194303 1103 88-89 2.1307497627216705 1103 90-91 2.363411864633253 1103 92-93 2.4745174444371543 1103 94-95 2.8822308546529136 1103 96-97 2.779233218422114 1103 98-99 3.146617093263618 1103 100-101 3.742976623068696 1104 1 0.02220060200486884 1104 2 -0.2109372782897445 1104 3 0.014346039997796822 1104 4 -0.06645525441261668 1104 5 0.1601337022238809 1104 6 -0.22208966755692927 1104 7 0.17774982961746133 1104 8 -0.08468306552288851 1104 9 0.2035710350915636 1104 10-11 0.014564916882889634 1104 12-13 -0.10040133355550296 1104 14-15 0.08507164795879874 1104 16-17 0.1457635040915548 1104 18-19 0.049889507838813074 1104 20-21 0.2141957897762623 1104 22-23 0.09649457374689518 1104 24-25 0.09685159339029781 1104 26-27 0.07903328140280053 1104 28-29 0.1462618371990274 1104 30-31 0.18238242670022942 1104 32-33 0.08715529593915505 1104 34-35 -0.01895080164731411 1104 36-37 0.2541436240404451 1104 38-39 0.505923615955048 1104 40-41 0.41512113123984307 1104 42-43 0.35500149834803807 1104 44-45 0.10054522442384695 1104 46-47 0.5286602578131365 1104 48-49 0.9320860783895455 1104 50-51 0.7593541619256925 1104 52-53 0.6923469269825873 1104 54-55 1.0327304789418292 1104 56-57 1.2004869084377177 1104 58-59 1.1443678154238057 1104 60-61 1.2606176918691077 1104 62-63 1.0412359160742142 1104 64-65 1.1692016245171786 1104 66-67 0.928965799105697 1104 68-69 1.0169408800149284 1104 70-71 1.3367008630526875 1104 72-73 1.0764054368233609 1104 74-75 1.085537415108142 1104 76-77 1.1932047704294142 1104 78-79 1.5734192798606053 1104 80-81 1.3980588864110395 1104 82-83 1.257688744695404 1104 84-85 1.504755425784822 1104 86-87 1.4987179707797722 1104 88-89 1.3568742479924971 1104 90-91 1.455706764854984 1104 92-93 1.3514773747507434 1104 94-95 1.518693477396024 1104 96-97 1.925495492733166 1104 98-99 2.50053299443562 1104 100-101 3.4763865058688204 1105 1 -0.22520199539772534 1105 2 -0.2460022133546822 1105 3 -0.238251362599609 1105 4 -0.2300916180489807 1105 5 -0.3645416224514477 1105 6 -0.2311805766478443 1105 7 -0.3241982223305868 1105 8 -0.2392285200683446 1105 9 -0.026948445427912304 1105 10-11 -0.35913638181840923 1105 12-13 -0.05072600888016865 1105 14-15 -0.2616815987944463 1105 16-17 -0.22566506733701175 1105 18-19 -0.3270585441092422 1105 20-21 -0.2065834310029544 1105 22-23 -0.27136256911025214 1105 24-25 -0.3395120429733396 1105 26-27 0.1972150995846178 1105 28-29 0.20502807096526254 1105 30-31 -0.01794224862444338 1105 32-33 0.03358386736773156 1105 34-35 0.0904647827682723 1105 36-37 0.29180596170278505 1105 38-39 0.3348197198511542 1105 40-41 0.3521341182528275 1105 42-43 0.4345469528934913 1105 44-45 0.7222984711770906 1105 46-47 0.9916472708001507 1105 48-49 0.5057873770908472 1105 50-51 0.3259126034841415 1105 52-53 0.5637754984111538 1105 54-55 0.7700681412794879 1105 56-57 1.001460934411746 1105 58-59 1.1222898933458794 1105 60-61 0.725877432128847 1105 62-63 1.185391760230054 1105 64-65 1.0208250011405582 1105 66-67 1.4172774874173797 1105 68-69 1.3429149059889482 1105 70-71 0.9688437201955402 1105 72-73 1.3111456965636208 1105 74-75 1.4796932592639855 1105 76-77 1.306191783416427 1105 78-79 1.3643283707696945 1105 80-81 1.114942003294157 1105 82-83 1.1235978356044924 1105 84-85 1.409625555654955 1105 86-87 1.5201465422083444 1105 88-89 1.2325235986418477 1105 90-91 1.0602522194004358 1105 92-93 1.2625163357897016 1105 94-95 1.3057064644090133 1105 96-97 1.8472487394864139 1105 98-99 2.087221306123933 1105 100-101 2.503009882492197 1106 1 -7.813180358731131E-4 1106 2 -0.13040506540459162 1106 3 -0.1177522538652056 1106 4 -0.2505016002236715 1106 5 -0.332545187513837 1106 6 -0.11692032709347444 1106 7 -0.05958146653735952 1106 8 0.06683208599226731 1106 9 0.14328328362734766 1106 10-11 -0.08961766524087267 1106 12-13 -0.2801823368659129 1106 14-15 -0.48498819415986816 1106 16-17 -0.16118200138335936 1106 18-19 -0.15023144963507917 1106 20-21 -0.27596845774092316 1106 22-23 0.12253226154929564 1106 24-25 -0.46018940482716886 1106 26-27 -0.46575281485387876 1106 28-29 -0.15228030693135253 1106 30-31 -0.18857504719841955 1106 32-33 -0.06490098111711973 1106 34-35 -0.10900045787343515 1106 36-37 -0.46545785291397124 1106 38-39 -0.9153585332682752 1106 40-41 -0.3400673077721237 1106 42-43 -0.19045304710650868 1106 44-45 -0.1293058068977686 1106 46-47 -0.04792492171322493 1106 48-49 -0.44055843396085237 1106 50-51 -0.6368770578349299 1106 52-53 -0.3304758384872386 1106 54-55 -0.19040422948699387 1106 56-57 -0.6186816680838021 1106 58-59 -1.1406780210926186 1106 60-61 -1.4547821044130416 1106 62-63 -1.865722321766377 1106 64-65 -0.9942819507311071 1106 66-67 -0.7661895357555224 1106 68-69 -0.5254897285921523 1106 70-71 -0.6341420195549041 1106 72-73 -0.3884532339176623 1106 74-75 -0.6679003236237158 1106 76-77 0.05378536630412967 1106 78-79 -0.4909746595333324 1106 80-81 -0.36540559028873076 1106 82-83 -0.4176677615434592 1106 84-85 0.038146054763686266 1106 86-87 0.2437205172529069 1106 88-89 0.2714719052372132 1106 90-91 0.07981549925783327 1106 92-93 0.19776589015690504 1106 94-95 0.22874568009529028 1106 96-97 1.075323605796573 1106 98-99 1.0374886858030727 1106 100-101 1.511209525985958 1107 1 0.0013973312015984618 1107 2 0.19602921201007462 1107 3 0.29637265535774304 1107 4 0.06485787690051126 1107 5 0.018398893822400453 1107 6 0.23638395758335662 1107 7 0.13449986970083927 1107 8 0.04214072796757051 1107 9 -0.05079805261085113 1107 10-11 0.25388269786733275 1107 12-13 0.18541316059233282 1107 14-15 0.4570052698924201 1107 16-17 0.36305547138352523 1107 18-19 0.5634577297403709 1107 20-21 0.3281528203999571 1107 22-23 0.5726048831905359 1107 24-25 0.0972724688111768 1107 26-27 0.040873909910096984 1107 28-29 0.1426543335915227 1107 30-31 0.1688142047986716 1107 32-33 -0.16057999346279672 1107 34-35 -0.04073611509928554 1107 36-37 -0.21318842662493864 1107 38-39 0.07011713848190482 1107 40-41 0.1996930408117521 1107 42-43 0.23238645906632627 1107 44-45 0.44676536231065 1107 46-47 -0.054733200580322716 1107 48-49 0.002252023555492144 1107 50-51 -0.2345531653149635 1107 52-53 -0.5241032894676252 1107 54-55 -0.4433998721885217 1107 56-57 -0.7599307603132885 1107 58-59 -1.061520432131104 1107 60-61 -0.7754974275793458 1107 62-63 -0.7327339412289788 1107 64-65 -0.7514533378044526 1107 66-67 -0.38819389305399454 1107 68-69 -0.5343858796451642 1107 70-71 -0.7740294560109682 1107 72-73 -0.34960065584940025 1107 74-75 0.09762255219327898 1107 76-77 0.07375631764762858 1107 78-79 -0.2641228076814848 1107 80-81 0.1417657967846182 1107 82-83 0.21069098936431274 1107 84-85 0.389816352512419 1107 86-87 0.6352419406370728 1107 88-89 0.5468894796743946 1107 90-91 0.7973173147988639 1107 92-93 0.9742727892128222 1107 94-95 1.1415089335448165 1107 96-97 1.4925349347726105 1107 98-99 1.516065301634594 1107 100-101 2.5913656625146473 1108 1 -0.08908241028485264 1108 2 0.12199656638906475 1108 3 -0.1722660056746541 1108 4 0.03391082246352539 1108 5 -0.3408686511274652 1108 6 0.05948446301048449 1108 7 -0.12297796607677469 1108 8 -0.3235482272068424 1108 9 -0.08750366202340132 1108 10-11 -0.14449330677265237 1108 12-13 -0.007498431088841073 1108 14-15 -0.025470494462538795 1108 16-17 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-2.181949584792111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 923.0 3 152.0 4 122.0 5 118.0 6 148.0 7 160.0 8 224.0 9 280.0 10 369.0 11 575.0 12 1010.0 13 1453.0 14 1799.0 15 2464.0 16 3604.0 17 4898.0 18 4025.0 19 2456.0 20 2123.0 21 2055.0 22 2134.0 23 2340.0 24 2577.0 25 2734.0 26 3066.0 27 3370.0 28 3903.0 29 4500.0 30 5508.0 31 6722.0 32 8576.0 33 11124.0 34 15362.0 35 23023.0 36 32025.0 37 52149.0 38 45168.0 39 4624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57352746731378 23.217910726369087 15.66410776924812 19.544454037069013 2 14.06997084548105 23.666083576287658 41.62293488824101 20.64101068999028 3 17.13279373002729 29.62531003864306 32.61334390769207 20.62855232363758 4 10.476353373813392 20.63744431400294 41.25584071278076 27.63036159940291 5 10.006103178706514 41.55292855393539 35.6600568334221 12.780911433936007 6 24.38186572992885 42.720917671470815 19.1364189207568 13.760797677843534 7 23.582871285974612 38.320465945782125 22.431217434889206 15.665445333354056 8 24.07968666215933 36.701404269538926 21.123104780111753 18.095804288189992 9 26.22124650295306 18.012900217594034 23.038933789244638 32.72691949020827 10-11 21.951935089638397 30.466174061891003 28.78609774247638 18.79579310599422 12-13 23.519410873197838 30.000777212140058 28.91054288267983 17.56926903198228 14-15 20.87717798467445 30.975177580552405 28.761832227178765 19.385812207594384 16-17 17.014939871388883 34.34946476793659 29.600373011093197 19.035222349581336 18-19 16.66015879213849 31.930546408915983 34.679200638743026 16.7300941602025 20-21 17.83063952133033 30.81571994715984 34.71287590333359 16.64076462817624 22-23 17.853534961068025 30.86427428003046 34.391464494972254 16.890726263929253 24-25 17.227733159262844 31.82129021980285 34.56795160218676 16.383025018747546 26-27 16.8873380906401 31.885094139109455 34.887132152231224 16.34043561801922 28-29 16.322851136747786 31.926467902563367 35.19430207843579 16.556378882253057 30-31 17.20881345526536 31.65200801223206 34.79453598413097 16.344642548371617 32-33 16.664950526595963 32.02822499956287 34.65722206787755 16.64960240596362 34-35 17.152243770326823 32.08752821955494 34.30040760500006 16.459820405118183 36-37 17.36326971496068 31.743068291318266 34.05571011159119 16.837951882129868 38-39 16.305899767474838 32.8004227023814 34.087372737638965 16.806304792504804 40-41 16.685056470944755 32.39882046241293 34.293229314384064 16.622893752258257 42-43 16.788603355428286 32.13125645947143 33.92339552557757 17.15674465952272 44-45 17.243697087197578 32.23897645902378 33.423990115820516 17.093336337958124 46-47 17.381979251661033 32.504565411664146 32.97451140381552 17.138943932859306 48-49 17.151194417245613 32.466079171912156 32.99023950513669 17.392486905705535 50-51 17.0465318682685 32.43398660304928 32.63253189935191 17.886949629330307 52-53 17.288675616342548 33.056553921473395 32.348804227459055 17.305966234725002 54-55 17.701877833943996 32.406525987776405 31.98417848164712 17.90741769663249 56-57 17.655959845535847 33.099267305346416 31.635626209179275 17.609146639938462 58-59 17.710716546963795 32.34055693881753 31.914831632633245 18.03389488158544 60-61 17.80036674151716 32.5152873715045 32.05064451713662 17.633701369841724 62-63 17.449347652539757 32.20850256296702 32.57366952001911 17.76848026447411 64-65 17.516248965621735 31.926255142753913 32.445678144218554 18.111817747405798 66-67 17.602177084284317 32.180367004197606 31.74312425580352 18.474331655714554 68-69 17.38459028239106 31.489574956586598 32.20322521745549 18.922609543566853 70-71 17.40461455383193 31.99321686227433 32.525067695403706 18.077100888490033 72-73 17.67730630782889 31.19161261371517 32.53531168107107 18.595769397384867 74-75 17.256800494228177 32.063441491077086 32.118614762346965 18.56114325234778 76-77 17.315380700207086 31.804795225754816 32.25820864788 18.621615426158108 78-79 17.26875721207082 31.641418752743988 31.728643528465582 19.36118050671961 80-81 17.238291342100663 31.437673614483575 31.65543231220498 19.668602731210786 82-83 17.492497304857086 31.463535444771423 31.827161213251365 19.21680603712013 84-85 17.55107035539956 31.105668095620096 31.601949385135647 19.741312163844693 86-87 17.509887104418702 30.75200267282037 31.84366331787138 19.894446904889552 88-89 17.94934554821264 30.292045953432716 31.809387274613332 19.94922122374131 90-91 17.633689657784778 29.971464258449515 32.46683618464121 19.9280098991245 92-93 18.126600080028282 29.938347144039252 32.395526185953095 19.539526589979374 94-95 17.742264456613164 30.17255278273656 32.17275091831958 19.912431842330697 96-97 18.39589104429482 30.550062356977183 31.180042659165697 19.874003939562296 98-99 18.287175084063893 30.68744937226465 30.605473720198837 20.41990182347262 100-101 18.72699621839681 29.74589258071596 31.604646691048654 19.922464509838576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 836.0 1 554.5 2 314.5 3 376.0 4 323.5 5 313.0 6 614.5 7 1145.0 8 1713.0 9 1938.0 10 1563.5 11 1146.0 12 1041.0 13 1028.5 14 988.5 15 925.5 16 900.5 17 929.0 18 988.5 19 1014.0 20 1085.0 21 1287.0 22 1489.5 23 1706.5 24 1971.0 25 2433.5 26 3457.5 27 4547.5 28 4893.5 29 5226.0 30 6229.5 31 7341.0 32 8312.0 33 9637.5 34 11426.5 35 12589.5 36 13655.0 37 14545.0 38 13807.0 39 12435.5 40 11167.0 41 10095.0 42 9227.0 43 8565.0 44 8053.0 45 7444.5 46 6763.0 47 6127.0 48 5995.5 49 5370.0 50 4200.5 51 3371.5 52 2789.5 53 2393.0 54 2043.0 55 1720.0 56 1425.0 57 1145.0 58 884.5 59 707.0 60 589.0 61 458.5 62 321.0 63 208.5 64 126.5 65 90.5 66 73.0 67 63.5 68 42.5 69 20.5 70 14.5 71 15.5 72 11.0 73 6.0 74 4.5 75 2.5 76 1.0 77 3.5 78 4.0 79 2.5 80 1.0 81 0.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2796058372081299 2 0.2377231320507401 3 0.2470303998634934 4 0.23927434335286568 5 0.24043775182945984 6 0.2001062579741956 7 0.19157459581250508 8 0.19584042689335035 9 0.19506482124228758 10-11 0.203014779165681 12-13 0.20669890600822916 14-15 0.19971845514866424 16-17 0.19312580711463065 18-19 0.18711486331889413 20-21 0.18730876473165983 22-23 0.19041118733591092 24-25 0.19235020146356785 26-27 0.19564652548058467 28-29 0.19622822971888174 30-31 0.19642213113164742 32-33 0.1948709198295219 34-35 0.19622822971888174 36-37 0.1919623986380365 38-39 0.18284903223804888 40-41 0.18381853930187736 42-43 0.18963558168484815 44-45 0.18692096190612845 46-47 0.1911867929869737 48-49 0.19351360994016203 50-51 0.19041118733591092 52-53 0.19312580711463065 54-55 0.19079899016144233 56-57 0.17722589126784377 58-59 0.16287718672318247 60-61 0.17877710256996934 62-63 0.17315396159976423 64-65 0.1791649053955007 66-67 0.17625638420401532 68-69 0.1760624827912496 70-71 0.17838929974443793 72-73 0.1847880463657058 74-75 0.19079899016144233 76-77 0.18692096190612845 78-79 0.18692096190612845 80-81 0.18149172234868904 82-83 0.17664418702954668 84-85 0.17392956725082698 86-87 0.17722589126784377 88-89 0.1830429336508146 90-91 0.18091001811039195 92-93 0.1760624827912496 94-95 0.1797466096337978 96-97 0.18381853930187736 98-99 0.1820734265869861 100-101 0.1677247220423248 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 257863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.5666270311166 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01588352319779 35.66038037543623 2 10.706511125152272 9.543061762383237 3 2.8951947652352366 3.8708719585403997 4 1.3429430713380759 2.394017719373849 5 0.7811762775729125 1.7407195904074004 6 0.5868974367529998 1.569362350157357 7 0.4479503641681215 1.397454576583347 8 0.3636770405095308 1.2966287219334833 9 0.2645753911707707 1.0612109501927567 >10 2.2543183865496013 20.347839364233582 >50 0.2219635654389635 6.849442719789672 >100 0.11296360026804393 8.822135216843217 >500 0.001981817548562174 0.5970629630661275 >1k 0.003963635097124348 4.849811731059328 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4011 1.5554771332063926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3046 1.1812474065686043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 0.8787612026541226 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1659 0.6433648875565707 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 0.28968871067194596 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 605 0.23462070944648902 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 485 0.1880843703827226 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 425 0.1648162008508394 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 407 0.15783574999127442 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.15589673586361752 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 382 0.14814067935298977 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 380 0.147365073701927 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 376 0.14581386239980143 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 366 0.14193583414448757 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 362 0.14038462284236203 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 342 0.13262856633173428 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 341 0.1322407635062029 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 341 0.1322407635062029 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 339 0.13146515785514012 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.12409690417004378 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 298 0.11556524200835327 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 296 0.11478963635729049 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.11323842505516495 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 263 0.10199214311475474 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.939014127656934E-4 0.0 12-13 0.0 0.0 0.0 3.878028255313868E-4 0.0 14-15 0.0 0.0 0.0 3.878028255313868E-4 0.0 16-17 0.0 0.0 0.0 3.878028255313868E-4 0.0 18-19 0.0 0.0 0.0 7.756056510627736E-4 0.0 20-21 0.0 0.0 0.0 7.756056510627736E-4 0.0 22-23 0.0 0.0 0.0 0.0011634084765941605 0.0 24-25 0.0 0.0 0.0 0.0025207183659540144 0.0 26-27 0.0 0.0 0.0 0.0034902254297824813 0.0 28-29 0.0 0.0 0.0 0.005429239557439416 0.0 30-31 0.0 0.0 0.0 0.011440183353175912 0.0 32-33 0.0 0.0 0.0 0.018420634212740872 0.0 34-35 0.0 0.0 0.0 0.022492563880820438 0.0 36-37 0.0 0.0 0.0 0.028503507676556933 0.0 38-39 0.0 0.0 0.0 0.03955588820420146 0.0 40-41 0.0 0.0 0.0 0.06534477610203868 0.0 42-43 0.0 0.0 0.0 0.11149331234027371 0.0 44-45 0.0 0.0 0.0 0.1533760174976635 0.0 46-47 0.0 0.0 0.0 0.2712680764592051 0.0 48-49 0.0 0.0 0.0 0.37752605065480505 0.0 50-51 0.0 0.0 0.0 0.45179029174406565 0.0 52-53 0.0 0.0 0.0 0.5404032373779876 0.0 54-55 0.0 0.0 0.0 0.604002900765135 0.0 56-57 0.0 0.0 0.0 0.8054664686286904 0.0 58-59 0.0 0.0 0.0 1.0459042204581503 0.0 60-61 0.0 0.0 0.0 1.3026296909599284 0.0 62-63 0.0 0.0 0.0 1.6008500637935648 0.0 64-65 0.0 0.0 0.0 2.015023481461086 0.0 66-67 0.0 0.0 0.0 2.5519364934092907 0.0 68-69 0.0 0.0 0.0 3.1468260277744386 0.0 70-71 0.0 0.0 0.0 3.56953110760365 0.0 72-73 0.0 0.0 0.0 3.9189414534074296 0.0 74-75 0.0 0.0 0.0 4.417655887040793 0.0 76-77 0.0 0.0 0.0 5.067613422631397 0.0 78-79 0.0 0.0 0.0 5.632060435192331 0.0 80-81 0.0 0.0 0.0 6.182158743208603 0.0 82-83 0.0 0.0 0.0 6.843750363565149 0.0 84-85 0.0 0.0 0.0 7.889072879785003 0.0 86-87 0.0 0.0 0.0 9.126357794642892 0.0 88-89 0.0 0.0 0.0 10.414057076819862 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGCAG 20 0.0020652951 71.31031 5 ATCACGC 20 0.0020700856 71.26868 3 TCACGCA 25 0.0050039315 57.03714 4 TATCACG 25 0.005007789 57.026043 2 GGTATCA 1785 0.0 43.94473 1 CATTCCG 90 1.1856173E-8 42.241512 9 GGCATTC 120 8.54925E-11 39.59371 7 GTCGGGA 25 0.0016121352 38.01366 12-13 GTCCTAC 55 0.002565247 34.574696 1 GTACAGG 70 2.0387518E-4 33.95729 1 GTATCAA 2770 0.0 33.123684 1 TAGGCAT 115 1.0234362E-7 33.07145 5 TTAGGCA 135 1.1819793E-8 31.6873 4 TACAGGG 90 2.39823E-5 31.681135 2 ATCGTGA 90 2.401424E-5 31.67497 7 TATCGTG 90 2.401424E-5 31.67497 6 ATGGGAT 335 0.0 29.801325 5 GTATAGG 150 3.288187E-8 28.524126 1 TGTATCG 100 4.91647E-5 28.51857 4 TATCAAC 3285 0.0 27.919905 2 >>END_MODULE