FastQCFastQC Report
Thu 26 May 2016
SRR937644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257863
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46601.8071611669762626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37011.4352582572916626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30211.1715523359303195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15540.6026455908757752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.39012964248457516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.2796058372081299No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6220.24121335748052258No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA6190.24004994900392843No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.23655972357414595No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5730.22221101902948465No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.188472173208254No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA4860.188472173208254No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA4850.1880843703827226No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4530.17567467996571823No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.1737356658380613No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.1706332432338102No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT4080.15822355281680583No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT3630.1407724256678934No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA3480.1349553832849226No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT3290.12758712959982627No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC2900.11246281940410219No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC2780.10780918549772554No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC2760.10703357984666276No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG2650.1027677487658175No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT2650.1027677487658175No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.10160434028922335No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2580.1000531289870978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC654.4052285E-851.1482056
GGTATCA14800.048.4762951
CTTACCC459.598037E-442.2339483
TATACGT802.2485801E-741.574045
CTATACG704.288977E-640.725594
GTATAGA608.259328E-539.5943261
GTATCAA25200.039.4057851
CATTCCG1152.4847395E-937.1770259
TAGGCAT1353.0559022E-1035.1949585
GCATTCC1456.5847416E-1032.7613648
TTAGGCA1657.4578566E-1131.675464
ACTATAC1051.8900137E-631.675463
CTACGCA600.003939958331.6754593
CCTTTAG1551.3424142E-930.6536711
CATGGGG6550.030.4664754
GGCATTC1601.88993E-929.6899837
ATCAACG33400.029.5890523
TCAACGC33600.029.412934
TATCAAC33900.029.2927932
CGCATTC650.00583892329.2275476
Produced by FastQC (version 0.11.5)