FastQCFastQC Report
Thu 26 May 2016
SRR937643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651921
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52310.8023978365476799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40160.6160255613793696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.4373229271644877No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14370.2204254810015324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12290.18851977463527023No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11120.17057281480424774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.14741049912489396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8040.12332782653112877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.11826586350186603No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.11105640100564332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG652.5831014E-643.857332
GTACCTG6350.038.1710621
TACCTGG6450.035.3578452
ATAACGC958.674906E-735.008923
TAACGCA902.4046409E-531.6796074
TGGACGT1051.8939481E-631.6796055
GTATAGG1401.6942067E-830.5528891
ATCACGC1004.94126E-528.5072633
GTATCAA61650.027.213191
TATAACG1109.454037E-525.9156952
ACCGTCT1301.001644E-525.5755798
ACCTGGG8750.025.520793
GGTATCA44350.025.1832661
GTACATG38750.025.1431661
TCGTACC950.001225809225.0006079
TCACGCA1151.2773694E-424.7927364
GAGTACT36400.024.33781612-13
GTCTAGA2402.2737368E-1023.763361
GACGTGC1000.001651904923.7524017
TACATGG39550.023.425692
Produced by FastQC (version 0.11.5)