##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651921 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.789920864644643 33.0 31.0 34.0 30.0 34.0 2 31.895205093868736 33.0 31.0 34.0 30.0 34.0 3 31.907712744335587 33.0 31.0 34.0 30.0 34.0 4 35.49675037312803 37.0 35.0 37.0 33.0 37.0 5 35.50941601819852 37.0 35.0 37.0 33.0 37.0 6 35.582995485649334 37.0 35.0 37.0 33.0 37.0 7 35.54159169592635 37.0 35.0 37.0 33.0 37.0 8 35.5363794079344 37.0 35.0 37.0 33.0 37.0 9 37.16598176772953 39.0 37.0 39.0 34.0 39.0 10-11 37.04224668326377 39.0 37.0 39.0 33.0 39.0 12-13 36.929131750626226 39.0 37.0 39.0 33.0 39.0 14-15 38.06050196266112 40.0 38.0 41.0 33.0 41.0 16-17 37.99705332394569 40.0 38.0 41.0 33.0 41.0 18-19 37.98386460936217 40.0 38.0 41.0 33.0 41.0 20-21 37.90745657832774 40.0 38.0 41.0 33.0 41.0 22-23 37.80814930029865 40.0 38.0 41.0 33.0 41.0 24-25 37.83750178319153 40.0 38.0 41.0 33.0 41.0 26-27 37.745946978238166 40.0 37.5 41.0 33.0 41.0 28-29 37.62674695246817 40.0 37.0 41.0 33.0 41.0 30-31 37.370432920553256 40.0 37.0 41.0 32.0 41.0 32-33 37.17763271930188 39.5 36.0 41.0 32.0 41.0 34-35 37.12965297942542 40.0 36.0 41.0 31.5 41.0 36-37 36.98408626198573 40.0 36.0 41.0 31.0 41.0 38-39 36.83641192721204 40.0 36.0 41.0 31.0 41.0 40-41 36.606253671840605 39.0 35.5 41.0 30.5 41.0 42-43 36.33041810280693 39.0 35.0 41.0 30.0 41.0 44-45 36.0539513223228 39.0 35.0 41.0 29.5 41.0 46-47 35.64729545451059 39.0 35.0 41.0 27.5 41.0 48-49 35.28489034714329 39.0 35.0 40.0 26.0 41.0 50-51 34.39892486973115 38.0 34.0 39.5 24.5 40.5 52-53 34.49320316418707 38.0 34.5 40.0 24.0 40.5 54-55 34.792332966724494 39.0 35.0 40.0 24.0 41.0 56-57 34.58509389941419 38.0 34.0 40.0 23.5 41.0 58-59 34.34860742329209 38.0 34.0 40.0 23.0 41.0 60-61 34.0366907953571 38.0 34.0 40.0 22.0 41.0 62-63 33.679632961662534 38.0 33.5 40.0 20.5 41.0 64-65 33.31465622368354 37.0 33.0 40.0 19.5 41.0 66-67 32.881155078606156 36.5 32.5 39.0 17.5 41.0 68-69 32.39302384798158 36.0 32.0 39.0 12.5 40.5 70-71 31.947252044342797 35.5 31.5 39.0 8.0 40.0 72-73 31.48454567347884 35.0 31.0 38.0 7.0 40.0 74-75 30.956199447479065 35.0 30.5 37.0 4.0 39.5 76-77 30.47667048614786 35.0 30.0 37.0 2.0 39.0 78-79 29.922730668286494 34.0 29.5 36.0 2.0 39.0 80-81 29.35841382621514 34.0 29.0 36.0 2.0 37.5 82-83 28.846547357731993 34.0 29.0 35.0 2.0 37.0 84-85 28.332031795263536 34.0 27.5 35.0 2.0 36.5 86-87 27.798299180422166 33.5 26.5 35.0 2.0 36.0 88-89 27.338965150685436 33.0 26.0 35.0 2.0 36.0 90-91 26.798518532153437 33.0 25.5 35.0 2.0 35.0 92-93 26.187229741026904 32.5 24.5 35.0 2.0 35.0 94-95 25.474517617932236 32.0 21.5 34.5 2.0 35.0 96-97 24.66989788640035 32.0 15.0 34.0 2.0 35.0 98-99 23.678567648534102 31.0 2.0 34.0 2.0 35.0 100-101 20.914121496316273 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20947856366159456 1101 2 0.2072656393205925 1101 3 0.22361852003086824 1101 4 0.08281244782146047 1101 5 -0.0421566605035224 1101 6 0.08241229367123992 1101 7 0.21927310476903017 1101 8 0.14951521975469007 1101 9 0.16635242018927698 1101 10-11 0.3589199755069359 1101 12-13 0.2753839219170757 1101 14-15 0.336899690554354 1101 16-17 0.4384043791749619 1101 18-19 0.35543043000163266 1101 20-21 0.4072973204610122 1101 22-23 0.4028376238629079 1101 24-25 0.3979970573717324 1101 26-27 0.3163763452465389 1101 28-29 0.38435506673832975 1101 30-31 0.2909551094561067 1101 32-33 0.3030233784341476 1101 34-35 0.5094935590039356 1101 36-37 0.2926610903471598 1101 38-39 0.48902449632855394 1101 40-41 0.40537576759764704 1101 42-43 0.4171180051635517 1101 44-45 0.6063657748886442 1101 46-47 0.6322152579300564 1101 48-49 0.7732923823248967 1101 50-51 0.43572776484732145 1101 52-53 0.43461191387921616 1101 54-55 0.7413374402106854 1101 56-57 0.5096075371143698 1101 58-59 0.4252932348929548 1101 60-61 0.593009884705765 1101 62-63 0.9205732475682922 1101 64-65 0.7383247641889383 1101 66-67 0.9239165968175698 1101 68-69 0.7395205682776194 1101 70-71 1.2185485590255745 1101 72-73 1.1883174131252616 1101 74-75 1.2613095443048437 1101 76-77 1.1657278636415462 1101 78-79 1.024184260367388 1101 80-81 1.1683126155232308 1101 82-83 1.254219125190442 1101 84-85 1.321142226781948 1101 86-87 1.3520192178922308 1101 88-89 1.4252041429943851 1101 90-91 1.378434832364043 1101 92-93 0.9441379360427611 1101 94-95 1.1188751946133806 1101 96-97 1.5017029917662548 1101 98-99 1.9583858854277558 1101 100-101 2.4624583189494054 1102 1 0.19767240938968555 1102 2 0.27427078029833396 1102 3 0.29181577178976426 1102 4 0.18797152010382234 1102 5 0.06226585761762493 1102 6 0.18880179458128765 1102 7 0.21426411071894336 1102 8 0.4132058670069867 1102 9 0.36436414970785336 1102 10-11 0.20604454043971998 1102 12-13 0.4826770853746254 1102 14-15 0.5093842835868969 1102 16-17 0.602845417588604 1102 18-19 0.41226537587767353 1102 20-21 0.6219283515303999 1102 22-23 0.5251247735473186 1102 24-25 0.44479911376282644 1102 26-27 0.35230464829813 1102 28-29 0.3133957368748881 1102 30-31 0.4620709038595763 1102 32-33 0.29788240069043326 1102 34-35 0.3466544194524701 1102 36-37 0.5542506423476752 1102 38-39 0.5926316958261637 1102 40-41 0.33639193560011904 1102 42-43 0.4146816287545718 1102 44-45 0.5336938501619244 1102 46-47 0.4963752988024268 1102 48-49 0.43863389013215937 1102 50-51 0.5232308249531172 1102 52-53 0.5691086196088051 1102 54-55 0.7248011606607037 1102 56-57 0.6100982184269768 1102 58-59 0.8007666199553753 1102 60-61 0.8369583259144804 1102 62-63 0.8735519279442414 1102 64-65 0.8986689861769932 1102 66-67 1.2957061466893123 1102 68-69 0.9997381284951743 1102 70-71 1.033989586795368 1102 72-73 1.0287885097607443 1102 74-75 1.0177804706387832 1102 76-77 1.0693222905236404 1102 78-79 1.2050796207148053 1102 80-81 0.9249144611661713 1102 82-83 1.1150763805682438 1102 84-85 1.2367886807845672 1102 86-87 1.305015992806812 1102 88-89 1.330767681434633 1102 90-91 1.3948199692971244 1102 92-93 1.0831902079717466 1102 94-95 1.0645521964547662 1102 96-97 1.4325882404597188 1102 98-99 1.4518409843623097 1102 100-101 1.697142356373167 1103 1 0.0858223683132806 1103 2 0.10081014351391815 1103 3 -0.00577574843786266 1103 4 0.09441550591743209 1103 5 0.16553393624386814 1103 6 0.058743886872946405 1103 7 0.019395655392031586 1103 8 0.22129892008570806 1103 9 0.2842190798779711 1103 10-11 0.28803905022792975 1103 12-13 0.2519870018192094 1103 14-15 0.35784341757611315 1103 16-17 0.39331469043609246 1103 18-19 0.40766381697812193 1103 20-21 0.4544247949150986 1103 22-23 0.34886192464836796 1103 24-25 0.47403950088287417 1103 26-27 0.3466040451763206 1103 28-29 0.413189353000881 1103 30-31 0.5421348227487073 1103 32-33 0.3588057180607436 1103 34-35 0.33556980206413556 1103 36-37 0.2502862686125269 1103 38-39 0.3927949995711302 1103 40-41 0.3138640337025862 1103 42-43 0.4423891647715621 1103 44-45 0.3200705107506252 1103 46-47 0.6779951116543259 1103 48-49 0.45815519976808616 1103 50-51 0.4460071889902295 1103 52-53 0.7286112945842547 1103 54-55 0.6168744169173408 1103 56-57 0.5662937421010028 1103 58-59 0.5894445609255783 1103 60-61 0.6637130897752641 1103 62-63 0.6589407511554128 1103 64-65 0.8037824973077008 1103 66-67 0.8434900247024189 1103 68-69 0.7043406892581956 1103 70-71 0.5820539865789662 1103 72-73 0.7054330686402608 1103 74-75 0.6289843097795824 1103 76-77 0.6135806109997297 1103 78-79 0.7487037616168841 1103 80-81 0.8031630034548272 1103 82-83 0.9813466432568454 1103 84-85 0.8418831565094145 1103 86-87 1.0768265604626528 1103 88-89 1.0157978375249606 1103 90-91 0.767732586765618 1103 92-93 0.8901120826586641 1103 94-95 1.1356724801824996 1103 96-97 1.7325571826922719 1103 98-99 1.937800869749129 1103 100-101 1.3176545089770109 1104 1 0.4363314904216473 1104 2 0.43275128284663467 1104 3 0.3050234680483044 1104 4 0.22302347379984155 1104 5 0.31812892110780666 1104 6 0.34620766554647986 1104 7 0.26418706911647405 1104 8 0.3900538777533029 1104 9 0.4501410846373304 1104 10-11 0.517827954765508 1104 12-13 0.522752546636525 1104 14-15 0.49667834545722656 1104 16-17 0.34962448597783435 1104 18-19 0.4232754110402581 1104 20-21 0.7112501878861366 1104 22-23 0.6822600111910333 1104 24-25 0.571242162239777 1104 26-27 0.5646382186825463 1104 28-29 0.7507579509320195 1104 30-31 0.6794763797396257 1104 32-33 0.8032555586059615 1104 34-35 0.7226381814531848 1104 36-37 0.6123538385864649 1104 38-39 0.9229821363460999 1104 40-41 0.9465555008484259 1104 42-43 0.8138075028804437 1104 44-45 0.7258869677787771 1104 46-47 0.7886563228631047 1104 48-49 1.0516183632861242 1104 50-51 1.0143562249768223 1104 52-53 0.8659123808193456 1104 54-55 0.981669715834343 1104 56-57 0.9387764610882599 1104 58-59 1.0766456843937604 1104 60-61 1.2613953295887725 1104 62-63 1.282858158436916 1104 64-65 1.0050901074286216 1104 66-67 1.235924826314779 1104 68-69 1.544369909143903 1104 70-71 1.0539166935868813 1104 72-73 1.0943489380330362 1104 74-75 1.272212338206245 1104 76-77 1.2418668363732728 1104 78-79 1.4766937410577405 1104 80-81 1.625281890347157 1104 82-83 1.36923860252708 1104 84-85 1.4004220812823753 1104 86-87 1.5447190863234646 1104 88-89 1.667761478597921 1104 90-91 1.5217351445173861 1104 92-93 1.8634843488591066 1104 94-95 1.8170441959806638 1104 96-97 1.796675001156654 1104 98-99 1.796050023910329 1104 100-101 1.6942611979835327 1105 1 -0.013624744160992464 1105 2 0.15153858291825273 1105 3 0.19599187737431834 1105 4 0.15338075875249757 1105 5 0.07489266742753387 1105 6 0.2154047690715828 1105 7 0.21541647947694287 1105 8 0.25788231731723954 1105 9 0.15825758022100445 1105 10-11 0.14512166050275255 1105 12-13 0.21584954671610035 1105 14-15 0.3014408186011721 1105 16-17 0.20286511619985248 1105 18-19 0.3045139723516215 1105 20-21 0.18625416656044536 1105 22-23 0.23584081806617974 1105 24-25 0.11655130523332957 1105 26-27 0.2545502899803864 1105 28-29 0.053186931595661235 1105 30-31 0.14338184643812468 1105 32-33 0.12022062584556181 1105 34-35 0.527335006471084 1105 36-37 0.5410082509362653 1105 38-39 0.5476499170415678 1105 40-41 0.4478565273463957 1105 42-43 0.4331737000635414 1105 44-45 0.3708897528507862 1105 46-47 0.4733722455176803 1105 48-49 0.7471781627609388 1105 50-51 0.5830062607848916 1105 52-53 0.6734718620002127 1105 54-55 0.702128059114365 1105 56-57 0.7282110914694258 1105 58-59 0.7811714264165275 1105 60-61 0.8838410206844998 1105 62-63 0.6329974120376107 1105 64-65 0.7056570685171906 1105 66-67 0.4694232746317937 1105 68-69 0.6403812881524189 1105 70-71 0.7575392765422428 1105 72-73 0.7941977484094167 1105 74-75 0.8815005533729519 1105 76-77 1.033569311108689 1105 78-79 0.9572957144801961 1105 80-81 0.6943914630375367 1105 82-83 0.9870551060680981 1105 84-85 0.859678467054632 1105 86-87 0.9522089407039864 1105 88-89 1.2269363015469175 1105 90-91 1.0743531122105523 1105 92-93 1.017544585988098 1105 94-95 1.026085733340416 1105 96-97 1.5066057722237325 1105 98-99 1.5764888716862515 1105 100-101 1.9961968230332694 1106 1 0.027238889038535774 1106 2 0.03305005703478159 1106 3 0.1202255827366372 1106 4 0.2016718230957224 1106 5 0.22378934237010384 1106 6 0.04215141036912229 1106 7 0.2661137437876846 1106 8 0.17919934634478096 1106 9 0.27466011404005997 1106 10-11 0.06664868094501486 1106 12-13 -0.19305130485129496 1106 14-15 -0.1614904759204805 1106 16-17 0.16561658381940703 1106 18-19 0.1562925184221129 1106 20-21 0.12802505548542342 1106 22-23 0.11283689667953922 1106 24-25 0.038195503205351145 1106 26-27 0.16752567109740824 1106 28-29 0.3103277998695546 1106 30-31 0.24029926528397993 1106 32-33 0.39669462635603026 1106 34-35 0.4822680644565551 1106 36-37 0.42264386362392514 1106 38-39 0.576255156385372 1106 40-41 0.6184693310218279 1106 42-43 0.4795818027156997 1106 44-45 0.6141491444557658 1106 46-47 0.7573722919246251 1106 48-49 0.7014592032045925 1106 50-51 0.7833891180603416 1106 52-53 0.5948329776231134 1106 54-55 0.6307077746398022 1106 56-57 0.7008379564484173 1106 58-59 0.40074703492755503 1106 60-61 0.4606700343049397 1106 62-63 0.8125238291896153 1106 64-65 0.8266248853025786 1106 66-67 0.42359061806637044 1106 68-69 0.606992653212636 1106 70-71 0.1994888321957653 1106 72-73 0.6419945788152432 1106 74-75 0.4163162017939541 1106 76-77 0.4614018748602291 1106 78-79 0.5080219831299857 1106 80-81 0.4178292893363498 1106 82-83 0.2286937352070204 1106 84-85 0.5027487503690189 1106 86-87 0.2744575891017895 1106 88-89 0.49113334542468223 1106 90-91 0.5133047793799577 1106 92-93 0.1195308031261817 1106 94-95 0.4790140114108148 1106 96-97 0.7825600149784471 1106 98-99 0.6269645428466575 1106 100-101 0.49219886493873943 1107 1 0.45744027896438766 1107 2 0.4856009063387141 1107 3 0.3142619734335881 1107 4 0.4151642382326415 1107 5 0.3444446001232606 1107 6 0.3175687185933782 1107 7 0.22031571142135675 1107 8 0.2789567580179053 1107 9 0.32332465428500257 1107 10-11 0.32707863264766246 1107 12-13 0.38868088840666104 1107 14-15 0.4522151525693232 1107 16-17 0.4881611419830776 1107 18-19 0.4044951372010459 1107 20-21 0.39746945233153497 1107 22-23 0.2740259466684023 1107 24-25 0.3030904509546133 1107 26-27 0.2765141683471768 1107 28-29 0.23080240153266374 1107 30-31 0.15455004433277253 1107 32-33 0.45215457725087305 1107 34-35 0.48064999297256605 1107 36-37 0.32664644862163783 1107 38-39 0.2677114452001206 1107 40-41 0.19742436528038354 1107 42-43 0.15388922650600279 1107 44-45 0.37535263714018186 1107 46-47 0.21124868692989196 1107 48-49 0.0797305349435149 1107 50-51 0.2272437511057177 1107 52-53 0.05324291028098571 1107 54-55 0.17974562184588194 1107 56-57 0.5041920888952873 1107 58-59 0.3757060935575609 1107 60-61 0.5539098736238302 1107 62-63 0.4199603486468604 1107 64-65 0.375009139337358 1107 66-67 0.33515481095223976 1107 68-69 0.536284642902018 1107 70-71 0.5312412207483383 1107 72-73 0.5555354626592575 1107 74-75 0.42762679073770116 1107 76-77 0.35530926046306277 1107 78-79 0.5562189717055688 1107 80-81 0.6257478031585002 1107 82-83 0.7423885928730307 1107 84-85 0.6232889561407084 1107 86-87 0.47107652959543245 1107 88-89 0.6220747399036561 1107 90-91 0.4604004512223341 1107 92-93 0.6213618431716164 1107 94-95 0.6605195277861391 1107 96-97 0.731459799806764 1107 98-99 0.795377388522784 1107 100-101 0.7926265156017571 1108 1 -0.16402325928272887 1108 2 -0.13186571886141962 1108 3 -0.17560982586839557 1108 4 -0.08928765482977497 1108 5 -0.16174888732735582 1108 6 -0.10975081559922728 1108 7 -0.03619395834378025 1108 8 -0.19596635611872415 1108 9 -0.17005337075617888 1108 10-11 -0.17779022944156253 1108 12-13 0.050482148528523396 1108 14-15 -0.10062818456511025 1108 16-17 0.030280772930929345 1108 18-19 0.07187940525914627 1108 20-21 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-0.8353327413368596 2316 86-87 -0.5876738998830788 2316 88-89 -0.9377889871220333 2316 90-91 -0.7230604985833402 2316 92-93 -0.4999362751547345 2316 94-95 -0.5955824136798427 2316 96-97 -0.654627996756517 2316 98-99 -0.566327183805857 2316 100-101 -0.6658892066583952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2689.0 3 506.0 4 486.0 5 513.0 6 559.0 7 672.0 8 894.0 9 1071.0 10 1357.0 11 1830.0 12 2535.0 13 3434.0 14 3677.0 15 4733.0 16 6100.0 17 8308.0 18 7121.0 19 4624.0 20 4471.0 21 4707.0 22 4935.0 23 5657.0 24 6089.0 25 6993.0 26 8160.0 27 9252.0 28 11059.0 29 13115.0 30 16128.0 31 19974.0 32 25186.0 33 32975.0 34 44776.0 35 63736.0 36 89516.0 37 125770.0 38 97847.0 39 10464.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.765357954877594 19.626369501610636 14.500333818421238 21.10793872509053 2 14.679037350422226 21.1178213817211 42.094465641517765 22.108675626338915 3 19.265808146204783 27.224704966890474 30.106756312032307 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31.378568810891917 20.215751669689894 24-25 20.59396142800288 27.679502998695416 31.711415325845945 20.015120247455755 26-27 20.476675594154262 27.725073332933825 31.69526996814685 20.102981104765067 28-29 20.10257592347145 28.127240246643233 31.84118019938915 19.92900363049617 30-31 20.357013052775876 27.931111587517837 31.72015868954341 19.991716670162877 32-33 20.080037067858616 28.228741882761373 31.47417736478569 20.217043684594323 34-35 20.51413861994775 28.10189027201475 31.424081758106652 19.959889349930844 36-37 20.496931629263376 27.949523227120892 31.451260997427916 20.102284146187817 38-39 20.33178031365108 28.223757744293327 31.63481888051991 19.809643061535684 40-41 20.452567249372926 28.210358112753852 31.492872699616264 19.84420193825695 42-43 20.32309070541829 28.316151308359082 30.97366686284242 20.387091123380205 44-45 20.330403634578282 28.507052252825392 30.756765852298003 20.40577826029832 46-47 20.59851106024528 28.734047760097674 30.29990095761462 20.367540222042425 48-49 20.562361935496266 28.841814095545438 30.152404003150274 20.443419965808026 50-51 20.537373159925682 29.097800747171217 30.01155641204141 20.35326968086169 52-53 20.622879385804804 29.450321430355476 29.690712142113334 20.236087041726385 54-55 20.344680023939528 29.23372769018134 29.779051069711198 20.64254121616794 56-57 20.63264164799946 29.47828611989417 29.516311912904804 20.372760319201568 58-59 20.74317960788411 29.220715371059754 29.64552551242243 20.39057950863371 60-61 20.67882434002976 29.231571645956222 29.941183203145705 20.148420810868316 62-63 20.687472441029563 29.19722159486355 30.062822929677957 20.052483034428935 64-65 20.48622580432054 29.261735067776325 29.910691651904852 20.341347475998287 66-67 20.43081456399652 29.43587135786784 29.60010385516199 20.53321022297365 68-69 20.351470221061323 29.445829412103635 29.69996350855628 20.502736858278755 70-71 20.469998440559586 29.52097524177088 29.483410396320643 20.525615921348894 72-73 20.46289930442197 29.45356219681286 29.452025856605683 20.631512642159482 74-75 20.279241630433113 29.871249251002503 29.266289735277397 20.583219383286984 76-77 20.321596319872413 29.54158196934149 29.41105974215138 20.725761968634718 78-79 20.411110872097133 29.598722845877983 29.20952272664415 20.780643555380735 80-81 20.406078190523818 29.788695229337925 29.15186949451093 20.653357085627327 82-83 20.549998233196547 29.75565876419012 29.120761784194944 20.573581218418386 84-85 20.393855503868348 29.653692289729676 29.030117187259957 20.922335019142018 86-87 20.38257364231523 29.60801679434228 29.128474721304098 20.880934842038396 88-89 20.582593927178063 29.375657246359555 29.09728052676495 20.944468299697427 90-91 20.37878357221762 29.41964100523451 29.29288816712323 20.90868725542464 92-93 20.633862320065138 29.381884380808998 29.246309126941455 20.737944172184413 94-95 20.286589782845326 29.437905162963947 29.092688457671006 21.182816596519718 96-97 20.25767240637636 29.36131902707619 29.134327028256084 21.246681538291362 98-99 20.427215700372876 29.46155181605466 28.841860901013845 21.269371582558623 100-101 20.504720473614196 29.14496907805602 29.300039631701676 21.05027081662811 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1333.0 1 832.0 2 776.5 3 1072.5 4 714.0 5 579.0 6 887.0 7 1622.5 8 2440.5 9 2673.5 10 2194.5 11 1695.0 12 1621.5 13 1681.5 14 1775.0 15 1827.5 16 1832.5 17 1885.0 18 1937.5 19 1957.5 20 2088.5 21 2417.5 22 2786.0 23 3251.0 24 3867.0 25 4586.5 26 5502.0 27 6657.5 28 7593.5 29 8618.5 30 9975.5 31 11126.0 32 12410.0 33 14091.0 34 15932.5 35 17391.0 36 19135.5 37 20495.0 38 21116.5 39 21709.0 40 21883.0 41 22338.5 42 22763.0 43 23291.5 44 24111.5 45 24780.5 46 24449.0 47 24517.0 48 26960.0 49 26207.0 50 22643.0 51 20127.0 52 17926.0 53 16529.5 54 15127.5 55 13389.5 56 11634.5 57 10028.0 58 8676.0 59 7515.0 60 6357.5 61 5121.5 62 3820.5 63 2758.0 64 2038.0 65 1508.0 66 1079.5 67 799.5 68 603.0 69 417.0 70 285.5 71 199.0 72 157.0 73 123.5 74 95.0 75 76.0 76 52.0 77 33.0 78 19.5 79 10.5 80 11.0 81 8.0 82 5.0 83 5.5 84 5.5 85 4.5 86 1.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 1.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2863843932010167 2 0.25677957912078303 3 0.25386511555848024 4 0.25954064986401726 5 0.26613654108396567 6 0.18253745469159607 7 0.18253745469159607 8 0.1848383469776246 9 0.1794695983102247 10-11 0.18782950694946168 12-13 0.17808906293860757 14-15 0.179009419853019 16-17 0.18652566798737885 18-19 0.17908611626255327 20-21 0.17256692145213914 22-23 0.172797010680742 24-25 0.17456102810003055 26-27 0.17210674299493342 28-29 0.18154040136765037 30-31 0.1866023643969131 32-33 0.18752272131132453 34-35 0.18729263208272168 36-37 0.16673799432753356 38-39 0.15929844260270798 40-41 0.16543415536545072 42-43 0.17548138501444194 44-45 0.17969968753882756 46-47 0.18246075828206176 48-49 0.18154040136765037 50-51 0.18407138288228175 52-53 0.20148146784656423 54-55 0.17095629685191918 56-57 0.16060228156479084 58-59 0.15983531746944799 60-61 0.15362290829717098 62-63 0.16014210310758514 64-65 0.15799460364062518 66-67 0.15561701494506236 68-69 0.16620111946079355 70-71 0.16052558515525653 72-73 0.15699755031667947 74-75 0.1612158528410651 76-77 0.16474388767964218 78-79 0.16857870815635637 80-81 0.15899165696457088 82-83 0.15768781800248802 84-85 0.1529326406113624 86-87 0.15147540883021102 88-89 0.15408308675437668 90-91 0.1607556743838594 92-93 0.152012283696951 94-95 0.1573043359548166 96-97 0.1560004969927338 98-99 0.1583013892787623 100-101 0.14219514327656266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 651921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.36217235319569 #Duplication Level Percentage of deduplicated Percentage of total 1 85.89062118743456 58.716694491384445 2 8.778600034863333 12.002483372061937 3 2.2164429553929716 4.545625659828023 4 0.9426906911729399 2.577775340228708 5 0.5081281553286267 1.7368372266043486 6 0.33274244157454524 1.3648197684085328 7 0.22859411318699624 1.0939033115230754 8 0.1697437431837885 0.9283240821925389 9 0.11858382739958326 0.729598324229273 >10 0.755232039752576 9.380436303498897 >50 0.03952979606940726 1.847264709080457 >100 0.01617121240168462 1.8742817844957078 >500 0.0015722012056145582 0.764329869978851 >1k 0.0011230008611532558 1.6344498726245216 >5k 2.2460017223065114E-4 0.8031758838608617 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5231 0.8023978365476799 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4016 0.6160255613793696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2851 0.4373229271644877 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1437 0.2204254810015324 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1229 0.18851977463527023 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1112 0.17057281480424774 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 961 0.14741049912489396 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 804 0.12332782653112877 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 771 0.11826586350186603 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.11105640100564332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 3.0678563813713624E-4 0.0 28-29 0.0 0.0 0.0 0.0014572317811513973 0.0 30-31 0.0 0.0 0.0 0.004141606114851339 0.0 32-33 0.0 0.0 0.0 0.0069793732676198495 0.0 34-35 7.669640953428406E-5 0.0 0.0 0.009050176325045518 0.0 36-37 1.5339281906856812E-4 0.0 0.0 0.012501514754088302 0.0 38-39 1.5339281906856812E-4 0.0 0.0 0.01902070956450245 0.0 40-41 1.5339281906856812E-4 0.0 0.0 0.02469624387003947 0.0 42-43 2.300892286028522E-4 0.0 0.0 0.03435999147135926 0.0 44-45 4.601784572057044E-4 0.0 0.0 0.046247934949173286 0.0 46-47 4.601784572057044E-4 0.0 0.0 0.05798248560791875 0.0 48-49 4.601784572057044E-4 0.0 0.0 0.07332176751477557 0.0 50-51 4.601784572057044E-4 0.0 0.0 0.09188229862207231 0.0 52-53 4.601784572057044E-4 0.0 0.0 0.12025997014975742 0.0 54-55 4.601784572057044E-4 0.0 0.0 0.13966416176193128 0.0 56-57 4.601784572057044E-4 0.0 0.0 0.1839179900632132 0.0 58-59 4.601784572057044E-4 0.0 0.0 0.2458119925573804 0.0 60-61 4.601784572057044E-4 0.0 0.0 0.3037177817557649 0.0 62-63 4.601784572057044E-4 0.0 0.0 0.3934525809108772 0.0 64-65 4.601784572057044E-4 0.0 0.0 0.46148229616778724 0.0 66-67 4.601784572057044E-4 0.0 0.0 0.566172895182085 0.0 68-69 4.601784572057044E-4 0.0 0.0 0.6992411657240678 0.0 70-71 4.601784572057044E-4 0.0 0.0 0.812138280558534 0.0 72-73 4.601784572057044E-4 0.0 0.0 0.9672951170463906 0.0 74-75 4.601784572057044E-4 0.0 0.0 1.1364873964790212 0.0 76-77 4.601784572057044E-4 0.0 0.0 1.2670246855063727 0.0 78-79 4.601784572057044E-4 0.0 0.0 1.4106003641545524 0.0 80-81 4.601784572057044E-4 0.0 0.0 1.641073074805076 0.0 82-83 4.601784572057044E-4 0.0 0.0 1.836802311936569 0.0 84-85 4.601784572057044E-4 0.0 0.0 2.101098139191712 0.0 86-87 4.601784572057044E-4 0.0 0.0 2.4482260887438816 0.0 88-89 4.601784572057044E-4 0.0 0.0 2.873814465249624 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 65 2.5831014E-6 43.85733 2 GTACCTG 635 0.0 38.171062 1 TACCTGG 645 0.0 35.357845 2 ATAACGC 95 8.674906E-7 35.00892 3 TAACGCA 90 2.4046409E-5 31.679607 4 TGGACGT 105 1.8939481E-6 31.679605 5 GTATAGG 140 1.6942067E-8 30.552889 1 ATCACGC 100 4.94126E-5 28.507263 3 GTATCAA 6165 0.0 27.21319 1 TATAACG 110 9.454037E-5 25.915695 2 ACCGTCT 130 1.001644E-5 25.575579 8 ACCTGGG 875 0.0 25.52079 3 GGTATCA 4435 0.0 25.183266 1 GTACATG 3875 0.0 25.143166 1 TCGTACC 95 0.0012258092 25.000607 9 TCACGCA 115 1.2773694E-4 24.792736 4 GAGTACT 3640 0.0 24.337816 12-13 GTCTAGA 240 2.2737368E-10 23.76336 1 GACGTGC 100 0.0016519049 23.752401 7 TACATGG 3955 0.0 23.42569 2 >>END_MODULE