FastQCFastQC Report
Thu 26 May 2016
SRR937643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651921
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73071.1208413289340273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61710.946587086472134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44550.683365008950471No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.37565901389892337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.21244905440996686No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.20631334164722412No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.20508619909467557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12450.19097405974036732No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9950.15262585497322528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT301.30002E-463.341063
TACGCTC405.3832156E-447.5057954
TACCTGG5750.042.135572
GTACCTG6150.039.401091
GGACCGA1255.646143E-934.1989256
GGTATCA41650.029.7741051
GTATCAA60650.029.769121
GCGACTG650.005843139329.229859
AGGACCG1703.6307028E-927.9445845
GTACATG33450.025.2834931
ACCTGGG8700.025.1180043
TACATGG33600.024.8839862
TATCAAC73450.024.3188272
ATCAACG73650.024.2527873
TCAACGC74300.024.0406154
GTCCTAT1601.8530536E-623.7565421
ACATGGG33000.023.7528973
CAACGCA75400.023.6898925
AACGCAG76650.023.3619526
ACCGAGT1703.1463787E-622.3522388
Produced by FastQC (version 0.11.5)