##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937643_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651921 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19353265196243 33.0 31.0 34.0 31.0 34.0 2 32.458136798783904 34.0 31.0 34.0 31.0 34.0 3 32.53891038944903 34.0 31.0 34.0 31.0 34.0 4 36.16844218854739 37.0 35.0 37.0 35.0 37.0 5 36.10718476625235 37.0 35.0 37.0 35.0 37.0 6 36.155093945432036 37.0 36.0 37.0 35.0 37.0 7 36.1114751634017 37.0 35.0 37.0 35.0 37.0 8 36.10027441975331 37.0 35.0 37.0 35.0 37.0 9 37.787376077776294 39.0 38.0 39.0 35.0 39.0 10-11 37.71680080868694 39.0 37.0 39.0 35.0 39.0 12-13 37.609828491489 39.0 37.0 39.0 35.0 39.0 14-15 38.79438382871544 40.0 38.0 41.0 35.0 41.0 16-17 38.764617185211094 40.0 38.0 41.0 35.0 41.0 18-19 38.80558227147154 40.0 38.0 41.0 35.0 41.0 20-21 38.74276024242201 40.0 38.0 41.0 35.0 41.0 22-23 38.669433873122664 40.0 38.0 41.0 34.5 41.0 24-25 38.68105874791578 40.0 38.0 41.0 34.5 41.0 26-27 38.537371859473765 40.0 38.0 41.0 34.0 41.0 28-29 38.38154929815116 40.0 38.0 41.0 34.0 41.0 30-31 38.3304242385197 40.0 38.0 41.0 34.0 41.0 32-33 38.16624637034242 40.0 37.0 41.0 34.0 41.0 34-35 37.98578432049282 40.0 37.0 41.0 34.0 41.0 36-37 37.80334887202591 40.0 37.0 41.0 33.0 41.0 38-39 37.66698188890985 40.0 37.0 41.0 33.0 41.0 40-41 37.46584785579848 40.0 36.0 41.0 33.0 41.0 42-43 37.21457737977454 40.0 36.0 41.0 33.0 41.0 44-45 36.85021267914364 40.0 36.0 41.0 31.5 41.0 46-47 36.33951966572637 40.0 36.0 41.0 30.5 41.0 48-49 35.901240334334986 39.0 35.0 41.0 29.0 41.0 50-51 35.627266187160714 39.0 35.0 41.0 27.5 41.0 52-53 35.39263729807753 39.0 35.0 40.5 27.0 41.0 54-55 35.17733820508927 39.0 35.0 40.0 26.5 41.0 56-57 34.92433439020986 38.5 35.0 40.0 26.0 41.0 58-59 34.681119338079306 38.0 34.5 40.0 26.0 41.0 60-61 34.36939368420407 38.0 34.0 40.0 24.5 41.0 62-63 34.0057146494744 38.0 34.0 40.0 23.0 41.0 64-65 33.61127575273691 37.0 33.5 40.0 22.0 41.0 66-67 33.1920224996587 36.5 33.0 39.0 21.0 41.0 68-69 32.759322065096846 36.0 32.5 39.0 19.5 40.0 70-71 32.36474511482219 35.5 32.0 39.0 18.0 40.0 72-73 31.873121129707435 35.0 31.5 38.0 15.0 40.0 74-75 31.401662164587428 35.0 31.0 37.0 10.5 39.0 76-77 30.15656114774643 34.0 29.5 36.0 8.0 38.5 78-79 30.73012911073581 34.5 31.0 36.0 7.5 39.0 80-81 30.515583943453272 34.0 31.0 36.0 4.5 38.0 82-83 30.13095451749522 34.0 30.5 35.5 2.0 37.0 84-85 29.729892885794442 34.0 30.0 35.0 2.0 37.0 86-87 29.353627203296107 34.0 30.0 35.0 2.0 36.0 88-89 28.897041972877084 34.0 29.0 35.0 2.0 36.0 90-91 28.343270120152596 34.0 29.0 35.0 2.0 35.5 92-93 27.89974628827726 34.0 28.0 35.0 2.0 35.0 94-95 27.428287323157253 33.0 27.0 35.0 2.0 35.0 96-97 26.879680206650807 33.0 26.0 35.0 2.0 35.0 98-99 26.00742037762244 32.5 24.5 34.5 2.0 35.0 100-101 23.25571119813597 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41633233868254393 1101 2 0.2952171322157895 1101 3 0.3932915820524556 1101 4 0.21450346206952986 1101 5 0.17807566385540952 1101 6 0.1419071096746123 1101 7 0.22308150946798122 1101 8 0.194488601095415 1101 9 0.19218542786004633 1101 10-11 0.227716991301115 1101 12-13 0.2143616353073341 1101 14-15 0.23747037811976668 1101 16-17 0.3244605297732548 1101 18-19 0.2613072138782897 1101 20-21 0.17347288103133707 1101 22-23 0.2474929994828372 1101 24-25 0.2582203251631441 1101 26-27 0.14916855884892044 1101 28-29 0.07887769256004873 1101 30-31 0.14461271457541613 1101 32-33 0.09447265097241342 1101 34-35 0.17878335286676617 1101 36-37 0.16644992358722988 1101 38-39 0.25287737486784323 1101 40-41 0.3585987109247526 1101 42-43 0.5110168099772139 1101 44-45 0.5977229729759586 1101 46-47 0.21246071546301692 1101 48-49 0.27773128608712483 1101 50-51 0.14966112506038343 1101 52-53 0.49017828060535606 1101 54-55 0.3958325103439293 1101 56-57 0.4569926983568635 1101 58-59 0.5117044754808262 1101 60-61 0.5957685215190551 1101 62-63 0.6406510833385184 1101 64-65 0.5419290562309911 1101 66-67 0.47382755434635015 1101 68-69 0.6705565134623015 1101 70-71 0.5521270397594193 1101 72-73 0.5091326064209589 1101 74-75 0.5293205797791991 1101 76-77 0.6495920952737855 1101 78-79 0.5752540756277433 1101 80-81 0.607167719059813 1101 82-83 0.5448908339895411 1101 84-85 0.6252022328178235 1101 86-87 0.3485903428939352 1101 88-89 0.5212696770787701 1101 90-91 0.3492405051045324 1101 92-93 0.36796324562813254 1101 94-95 0.24503077790734196 1101 96-97 0.11679154503602618 1101 98-99 0.35039382697129895 1101 100-101 0.07123427725793263 1102 1 0.30199188461606497 1102 2 0.16630951015748252 1102 3 0.13142741881842568 1102 4 0.07668481192210663 1102 5 0.04128946679524148 1102 6 0.0473663384215115 1102 7 0.1327344136414368 1102 8 0.025451549866588152 1102 9 0.2246832192619479 1102 10-11 0.2510844913809436 1102 12-13 0.2488615341907945 1102 14-15 0.25271130157986477 1102 16-17 0.1907057001280137 1102 18-19 0.3027905402931239 1102 20-21 0.31975445334031605 1102 22-23 0.1901950462944768 1102 24-25 0.12786514001343363 1102 26-27 0.1567097239243438 1102 28-29 0.16653934597512432 1102 30-31 0.30127842329591914 1102 32-33 0.2580174775309345 1102 34-35 0.23007391922583054 1102 36-37 0.23056313153605856 1102 38-39 0.3987087124800013 1102 40-41 0.45823894892115646 1102 42-43 0.5389004940252846 1102 44-45 0.6493317903382021 1102 46-47 0.7034741160654647 1102 48-49 0.45746859596142286 1102 50-51 0.3245395191168612 1102 52-53 0.40612595545714214 1102 54-55 0.37637555938012923 1102 56-57 0.2845815478635174 1102 58-59 0.13983616114674646 1102 60-61 -0.056489570465068084 1102 62-63 0.22970601622907338 1102 64-65 0.09021912232928031 1102 66-67 0.16131890622126832 1102 68-69 0.5116981724121814 1102 70-71 0.43845608362271093 1102 72-73 0.21668973726576368 1102 74-75 0.22914176316202273 1102 76-77 -0.06103046329397799 1102 78-79 0.032133725658077594 1102 80-81 0.22579977193844414 1102 82-83 0.10083063239509116 1102 84-85 0.20229880443494253 1102 86-87 0.11141883859914259 1102 88-89 -0.03333112546723882 1102 90-91 0.17603532368016772 1102 92-93 0.0640058635885552 1102 94-95 0.08149340204119682 1102 96-97 -0.30469838056348664 1102 98-99 -0.489745181660858 1102 100-101 -0.42824098112143716 1103 1 0.2612120340940933 1103 2 0.16410541053217997 1103 3 0.23622834854772634 1103 4 0.11819001159349796 1103 5 0.18069676436872584 1103 6 0.1838381760394796 1103 7 0.15165761078812778 1103 8 0.1759795319131925 1103 9 0.1175058693911879 1103 10-11 0.3217298918257683 1103 12-13 0.38684618564249007 1103 14-15 0.2615116702656266 1103 16-17 0.2051535731718772 1103 18-19 0.16939642724285164 1103 20-21 0.25342407884375007 1103 22-23 0.17737419951946976 1103 24-25 0.30504469398382383 1103 26-27 0.16901761660472658 1103 28-29 0.2597467116753265 1103 30-31 0.1808283862269704 1103 32-33 0.3269426784045564 1103 34-35 0.28884122641721177 1103 36-37 0.18690192168573105 1103 38-39 0.3675657866379325 1103 40-41 0.21593626593080018 1103 42-43 0.07899206434608175 1103 44-45 -0.2023854036426087 1103 46-47 -0.23744399161481766 1103 48-49 -0.2932226898301522 1103 50-51 -0.18408652532580305 1103 52-53 -0.18789191778709124 1103 54-55 -0.11482307007064207 1103 56-57 -0.1660987174299322 1103 58-59 -0.14283360461756445 1103 60-61 -0.35718686743743433 1103 62-63 -0.21473263967090617 1103 64-65 -0.11395664459720223 1103 66-67 -0.16083530060343065 1103 68-69 -0.31181756686286377 1103 70-71 -0.6004212281772361 1103 72-73 -0.40524005214253833 1103 74-75 -0.3456483464418376 1103 76-77 -0.2223425036891129 1103 78-79 -0.07228749781758737 1103 80-81 -0.19046347770589378 1103 82-83 -0.26634631673028153 1103 84-85 -0.5031302936875619 1103 86-87 -0.16675356206609138 1103 88-89 0.0031236804718091093 1103 90-91 -0.1892070270922659 1103 92-93 -0.1175127610532769 1103 94-95 -0.07637022430380469 1103 96-97 -0.21519089664339575 1103 98-99 -0.07861948787041229 1103 100-101 -0.10424534924614903 1104 1 0.2716453949475408 1104 2 0.15403223940563748 1104 3 0.2184212159817136 1104 4 0.08323137270595993 1104 5 0.1612748486450286 1104 6 0.17920427449673326 1104 7 0.1634305536596088 1104 8 0.21874497960069306 1104 9 0.3575622046833047 1104 10-11 0.2803444969121358 1104 12-13 0.26424812500111017 1104 14-15 0.3996869248266748 1104 16-17 0.49313942459563265 1104 18-19 0.3537407200568694 1104 20-21 0.1895126612341116 1104 22-23 0.2283187869605925 1104 24-25 0.22903656745604906 1104 26-27 0.20723617487451662 1104 28-29 0.3448304051983868 1104 30-31 0.361720523568529 1104 32-33 0.38262436781578657 1104 34-35 0.4592312026204084 1104 36-37 0.3220944815419813 1104 38-39 0.38020885075980004 1104 40-41 0.3860894882123773 1104 42-43 0.7030652840375282 1104 44-45 0.7172538400399162 1104 46-47 0.20656269415404438 1104 48-49 0.47775127937969586 1104 50-51 0.6679969091844171 1104 52-53 0.8963742664681575 1104 54-55 0.8295579701896614 1104 56-57 0.8672066910818828 1104 58-59 0.892276799604339 1104 60-61 0.7460439130006193 1104 62-63 1.0060414040051384 1104 64-65 0.8145376004613141 1104 66-67 0.8243260967708324 1104 68-69 0.7988618561860292 1104 70-71 0.41527940154772125 1104 72-73 0.532305477457772 1104 74-75 0.6505089552919294 1104 76-77 0.43708596829702984 1104 78-79 0.517286426065013 1104 80-81 0.7366952324101739 1104 82-83 0.7747234062878654 1104 84-85 0.8887621997966448 1104 86-87 0.6944904280266684 1104 88-89 0.5316919870135024 1104 90-91 0.5624947681904615 1104 92-93 0.5792601008766916 1104 94-95 0.7382878787509277 1104 96-97 0.991897960948112 1104 98-99 0.8797097983873385 1104 100-101 0.5939460126757261 1105 1 0.2435559543491479 1105 2 0.041065739843290316 1105 3 0.06703265864901908 1105 4 0.05861133610215319 1105 5 -0.03944529140430575 1105 6 0.06139174963001892 1105 7 0.12180168439884653 1105 8 -0.05679015209342708 1105 9 -0.09777319756066305 1105 10-11 0.022629847433343286 1105 12-13 0.12239644997922738 1105 14-15 0.16685490445586737 1105 16-17 0.15370678355163392 1105 18-19 0.11885968241860922 1105 20-21 -0.15389865411561487 1105 22-23 0.04481437063211047 1105 24-25 0.006767927366929882 1105 26-27 0.020666189993484352 1105 28-29 -0.1374545474553841 1105 30-31 -0.06130406463868354 1105 32-33 0.11991051407185438 1105 34-35 0.06935295005416009 1105 36-37 0.06276051162075902 1105 38-39 -0.03378414677342789 1105 40-41 0.2289815865652045 1105 42-43 0.1626717931486965 1105 44-45 -0.05178610585011256 1105 46-47 -0.3369819067849278 1105 48-49 -0.25544876836439556 1105 50-51 -0.15235400988934344 1105 52-53 -0.17043762181066313 1105 54-55 -0.3596876731619645 1105 56-57 -0.23413213282199052 1105 58-59 -0.12462261950921061 1105 60-61 -0.25056028847944845 1105 62-63 -0.45153598121010674 1105 64-65 -0.3765640944539399 1105 66-67 -0.060704141153891555 1105 68-69 -0.08979892204265383 1105 70-71 -0.3683941084387463 1105 72-73 -0.33335458460954825 1105 74-75 -0.1317958907333754 1105 76-77 -0.19325739129555686 1105 78-79 -0.08820016580257573 1105 80-81 -0.5208662381078497 1105 82-83 -0.5198775326792671 1105 84-85 -0.42686006486476913 1105 86-87 -0.3688480775832659 1105 88-89 -0.3052261318692864 1105 90-91 -0.3250579492440906 1105 92-93 -0.2162758265522875 1105 94-95 -0.15209765478929782 1105 96-97 -0.00822935662967339 1105 98-99 -0.011300387018884805 1105 100-101 0.0523509621465621 1106 1 -0.04854094334674386 1106 2 -9.696686538163135E-4 1106 3 -0.09123821867422777 1106 4 -0.08439397749275912 1106 5 0.016004043816238323 1106 6 -0.1458894996448663 1106 7 0.04004193314005988 1106 8 -0.1538873978413875 1106 9 0.048749762274127306 1106 10-11 -0.17458341567081703 1106 12-13 -0.23148201023841608 1106 14-15 -0.20213829692706042 1106 16-17 -0.04292755294510897 1106 18-19 0.07023100756885015 1106 20-21 0.04620576150496447 1106 22-23 0.1340862457046157 1106 24-25 0.03207131275338071 1106 26-27 0.09515513353943561 1106 28-29 0.1402619989399554 1106 30-31 0.13444970012452728 1106 32-33 0.12456048970821598 1106 34-35 -0.1098219345085738 1106 36-37 0.41257140333016196 1106 38-39 0.3476205913754029 1106 40-41 0.19433300313712465 1106 42-43 0.1088455407413349 1106 44-45 0.30115377395590315 1106 46-47 -0.08867343983826714 1106 48-49 -0.1616429350117201 1106 50-51 0.15017516847574086 1106 52-53 0.3610204843220117 1106 54-55 -0.02768660580229465 1106 56-57 0.0010675330480225398 1106 58-59 0.20270294839574632 1106 60-61 -0.18065519067682345 1106 62-63 -0.09949871643529917 1106 64-65 -0.26462010763351174 1106 66-67 -0.16742154607766935 1106 68-69 0.21637784200124344 1106 70-71 0.0859774255427368 1106 72-73 0.07636070879834733 1106 74-75 0.24406785848350765 1106 76-77 0.2455063277568179 1106 78-79 -0.13679635579260108 1106 80-81 -0.34713256755073374 1106 82-83 -0.4309328265512349 1106 84-85 -0.3680149016283494 1106 86-87 -0.14735122165361503 1106 88-89 -0.31188436794140273 1106 90-91 -0.3033162966928664 1106 92-93 -0.21573054497816457 1106 94-95 0.1736639991541331 1106 96-97 0.3985124119389063 1106 98-99 -0.017044406324171035 1106 100-101 -0.6854764968413001 1107 1 0.15650616676779094 1107 2 0.05463960502547138 1107 3 0.1441312083424222 1107 4 0.05559980480441595 1107 5 0.12850774628692108 1107 6 0.10687235988890365 1107 7 0.06348805821379955 1107 8 0.1563735253650833 1107 9 0.1674320009408916 1107 10-11 0.0754477952390431 1107 12-13 0.24345624111833075 1107 14-15 0.36616218947354895 1107 16-17 0.15837057611383187 1107 18-19 0.25366180869782795 1107 20-21 0.2651227551722073 1107 22-23 0.27927327861193163 1107 24-25 0.419508860334723 1107 26-27 0.37756613348802404 1107 28-29 0.2837465073130474 1107 30-31 0.4071596149126222 1107 32-33 0.30605621442554565 1107 34-35 0.1126298476955796 1107 36-37 0.37517706143973584 1107 38-39 0.2804510414734409 1107 40-41 0.17964918801342122 1107 42-43 0.2303893914577131 1107 44-45 0.053541102571287524 1107 46-47 -0.045975691185233813 1107 48-49 0.056518877032502246 1107 50-51 -0.04914994149681462 1107 52-53 -0.11159347221770588 1107 54-55 -0.07515656377159985 1107 56-57 0.10254827652861565 1107 58-59 0.11958033970222459 1107 60-61 0.12725818252488352 1107 62-63 0.054925521834910285 1107 64-65 0.18368850584324292 1107 66-67 -0.0400859296560796 1107 68-69 0.055340562711329255 1107 70-71 -0.14765765405120135 1107 72-73 -0.0511621730824352 1107 74-75 -0.2627336719526383 1107 76-77 -0.05137429479679767 1107 78-79 -0.2153719633037774 1107 80-81 -0.08878579108694495 1107 82-83 -0.10838704443030878 1107 84-85 0.11254620933316772 1107 86-87 -0.13540417494006363 1107 88-89 -0.2945225343555258 1107 90-91 -0.4336530562905274 1107 92-93 -0.35902533997756336 1107 94-95 -0.33520357111937216 1107 96-97 -0.07595951170574367 1107 98-99 -0.16590453493759583 1107 100-101 0.2236911877498109 1108 1 -0.06367190895470287 1108 2 -0.06457898119612082 1108 3 0.027421347370491844 1108 4 -0.0018428619544010871 1108 5 -0.021506651814831912 1108 6 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-0.08780552452355295 2316 58-59 -0.18509506378447327 2316 60-61 0.1662671022916058 2316 62-63 -0.0930895065664501 2316 64-65 -0.18196949985934197 2316 66-67 -0.3531501082477462 2316 68-69 -0.20274144202743116 2316 70-71 -0.1221499769833514 2316 72-73 -0.19068022010419483 2316 74-75 -0.03993360591334394 2316 76-77 0.028556676293160166 2316 78-79 -0.16994317641882617 2316 80-81 -0.26444361830494856 2316 82-83 -0.34764896608450613 2316 84-85 -0.4867213345851944 2316 86-87 -0.22311149593118884 2316 88-89 -0.1102762524123655 2316 90-91 0.2815803792252254 2316 92-93 0.1985403981230931 2316 94-95 -0.03175971121191523 2316 96-97 0.025529049663944647 2316 98-99 0.2722951803795013 2316 100-101 0.5837090808286227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 17.0 6 26.0 7 84.0 8 160.0 9 330.0 10 593.0 11 983.0 12 1664.0 13 2563.0 14 3121.0 15 4501.0 16 6310.0 17 9816.0 18 8791.0 19 4701.0 20 3592.0 21 3565.0 22 3702.0 23 3887.0 24 4210.0 25 4962.0 26 5851.0 27 7062.0 28 8822.0 29 10879.0 30 13861.0 31 17196.0 32 21949.0 33 28939.0 34 40898.0 35 60122.0 36 91335.0 37 146155.0 38 120904.0 39 10365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93216916897566 19.861368092568558 15.539170110985307 20.667292627470474 2 15.09001857587039 21.30196756969019 41.544297545254715 22.063716309184702 3 19.152780781720484 26.82012084286286 31.22295492858797 22.804143446828682 4 11.983065406798382 17.96846238802307 38.30592710762057 31.742545097557983 5 12.03079854968169 38.52503916565268 34.80763948879809 14.636522795867544 6 29.045237076271512 37.63370101592064 18.38719722174926 14.933864686058588 7 26.35856185028554 34.66877121614429 21.548930008390588 17.423736925179583 8 25.906819998128604 35.12649538824489 20.310896565688175 18.655788047938323 9 26.258856517890973 17.086579508866873 21.443702534509548 35.21086143873261 10-11 23.998613328915617 27.8555223715757 27.63663081876485 20.509233480743834 12-13 25.65466027921332 25.402782315773976 28.905604054590945 20.036953350421765 14-15 22.285752414786455 26.16106859573476 28.70110028669118 22.852078702787608 16-17 19.933857016417633 29.693014951198077 29.224706674581736 21.148421357802555 18-19 19.656446103132126 27.24371511272071 33.44508000202479 19.65475878212237 20-21 20.544360436310534 26.03965817944199 33.88945899886643 19.52652238538105 22-23 20.1454017223025 26.133041272061824 33.95265969032915 19.76889731530653 24-25 20.01521661433399 26.276577304849226 34.17694529535111 19.53126078546568 26-27 20.033133153862085 26.345075661704364 34.13351433852573 19.488276845907826 28-29 19.558514709784895 26.56366723780627 34.3900949973386 19.487723055070234 30-31 19.826021864612432 26.4519780770983 34.17707053461999 19.54492952366928 32-33 19.65130744369333 26.578450456420335 33.972137728344386 19.79810437154195 34-35 20.083415015009486 26.398597376062437 33.89850917519147 19.619478433736603 36-37 20.0789666232565 26.21897438493314 34.03173083855253 19.67032815325783 38-39 19.91675371709149 26.656680794145306 33.991005045089814 19.43556044367339 40-41 19.91185974255866 26.69533091328554 33.94327945876421 19.449529885391588 42-43 19.78251498402267 26.819904857017065 33.468331438757644 19.92924872020262 44-45 19.915526574538937 26.834769857083913 33.113061245150874 20.13664232322628 46-47 20.180512669479892 27.21411029864048 32.552487187864784 20.052889844014842 48-49 20.231669174639258 27.517444598348572 32.106957744880134 20.143928482132036 50-51 20.309285941087953 27.685639824457258 31.835452455128767 20.16962177932602 52-53 20.32892022192873 28.045192592353978 31.41730363034785 20.20858355536944 54-55 20.138330174707423 27.883853579856993 31.568209829909723 20.409606415525865 56-57 20.48699152197889 28.0711926751861 31.183456277677816 20.25835952515719 58-59 20.481929558949627 27.794778815224547 31.321433118429994 20.401858507395833 60-61 20.39211806338498 27.939888422063408 31.811983353811275 19.856010160740333 62-63 20.434761267086042 27.892566737380758 31.648696697912783 20.023975297620417 64-65 20.26449523791993 27.843634428097886 31.679451958136035 20.212418375846152 66-67 20.172536242888324 28.10148775695215 31.393297654163614 20.332678345995912 68-69 20.150447676942452 28.064750176785225 31.397669349507073 20.387132796765254 70-71 20.32132727738484 28.085841689407154 31.284158663396333 20.308672369811678 72-73 20.30023576476291 27.926313157575844 31.17709047568647 20.596360601974776 74-75 20.184209577701576 28.51290916607163 30.68978502746884 20.613096228757954 76-77 20.161798745553526 28.11989489524037 31.096942727723142 20.621363631482957 78-79 20.122606880281506 28.186007200258928 30.933425982596052 20.757959936863514 80-81 20.07789287352302 28.505064263921547 30.843384397802804 20.573658464752633 82-83 20.195653591136917 28.337941534013122 30.95706128459506 20.509343590254904 84-85 20.069072786426574 28.325211183563653 30.675649350151325 20.93006667985845 86-87 20.00487789164638 28.131936231537257 30.850440467479956 21.012745409336407 88-89 20.130276521234936 27.955381096789335 30.758328079629283 21.15601430234645 90-91 19.955418541805855 27.9429553702171 31.123334619435276 20.97829146854177 92-93 20.176542638905186 27.855280747894074 30.88675516040929 21.08142145279145 94-95 19.68835180949839 27.989587695441625 30.93802776716811 21.384032727891878 96-97 19.759909559593876 27.917876552527073 30.939484998949258 21.382728888929794 98-99 19.800755316668507 28.02993348795543 30.63473290873615 21.534578286639913 100-101 19.47336848710423 27.99081931197003 31.156499739952103 21.379312460973637 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 912.0 1 708.5 2 1228.0 3 1674.5 4 1000.0 5 636.5 6 988.5 7 1939.5 8 2895.5 9 3207.0 10 2719.5 11 2053.5 12 1911.0 13 2011.0 14 2093.0 15 2110.5 16 2120.5 17 2143.5 18 2224.5 19 2328.5 20 2446.5 21 2747.5 22 3076.5 23 3426.5 24 3946.5 25 4685.0 26 5665.0 27 6723.0 28 7603.0 29 8649.5 30 9957.0 31 10931.5 32 12016.0 33 13498.5 34 15403.5 35 17100.0 36 18850.5 37 20257.5 38 20990.0 39 21412.0 40 21636.5 41 22354.5 42 22717.0 43 23172.5 44 24253.0 45 24907.5 46 24420.0 47 24610.5 48 27162.5 49 26220.0 50 22558.5 51 20130.5 52 17681.0 53 16120.0 54 14718.5 55 13111.0 56 11377.5 57 9714.0 58 8411.5 59 7269.0 60 6029.5 61 4746.0 62 3559.5 63 2556.0 64 1817.5 65 1302.0 66 930.5 67 732.5 68 554.0 69 378.0 70 247.5 71 159.5 72 128.5 73 110.5 74 81.5 75 67.0 76 44.5 77 30.0 78 22.0 79 11.0 80 10.0 81 10.5 82 6.0 83 3.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01886731674543388 2 0.0 3 0.0 4 1.5339281906856812E-4 5 0.03098534945185076 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002761070743234226 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.6017845720570434E-4 24-25 3.0678563813713624E-4 26-27 9.203569144114087E-4 28-29 0.001227142552548545 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.436605048771248E-4 42-43 0.009203569144114087 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.8348204767142034E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.669640953428406E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.368748667399885E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012348121935019734 92-93 0.0016106246002199655 94-95 0.0 96-97 0.0 98-99 0.001380535371617113 100-101 0.01810035265009104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 651921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.0119938891839 #Duplication Level Percentage of deduplicated Percentage of total 1 84.77939852703958 55.11677738968582 2 9.279280911220482 12.065291077925732 3 2.515597369663239 4.906320024725808 4 1.0695742348691986 2.7814061448537952 5 0.5553267145616061 1.805144848678985 6 0.35040174887887743 1.3668189814123755 7 0.24405000969188584 1.1106324417120905 8 0.18147736606738402 0.9438564331038365 9 0.1397512942329555 0.8176959258010599 >10 0.8087578313031514 9.686441849420468 >50 0.049410942571559945 2.181416951362649 >100 0.02363125610722213 2.637185699913465 >500 0.0014321973398022916 0.5937314633249137 >1k 0.0014321973398022916 1.8957196957598395 >5k 4.773991132674305E-4 2.091561072319181 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7307 1.1208413289340273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6171 0.946587086472134 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4455 0.683365008950471 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2449 0.37565901389892337 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1385 0.21244905440996686 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.20631334164722412 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1337 0.20508619909467557 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1245 0.19097405974036732 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 995 0.15262585497322528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.5339281906856812E-4 0.0 0.0 0.0 0.0 12-13 1.5339281906856812E-4 0.0 0.0 0.0 0.0 14-15 1.5339281906856812E-4 0.0 0.0 0.0 0.0 16-17 1.5339281906856812E-4 0.0 0.0 0.0 0.0 18-19 1.5339281906856812E-4 0.0 0.0 0.0 0.0 20-21 1.5339281906856812E-4 0.0 0.0 0.0 0.0 22-23 1.5339281906856812E-4 0.0 0.0 0.0 0.0 24-25 1.5339281906856812E-4 0.0 0.0 0.0 0.0 26-27 1.5339281906856812E-4 0.0 0.0 5.368748667399884E-4 0.0 28-29 2.300892286028522E-4 0.0 0.0 0.0016873210097542494 0.0 30-31 3.0678563813713624E-4 0.0 0.0 0.004525088162522759 0.0 32-33 3.0678563813713624E-4 0.0 0.0 0.007439551724825555 0.0 34-35 3.0678563813713624E-4 0.0 0.0 0.009510354782251224 0.0 36-37 3.0678563813713624E-4 0.0 0.0 0.012884996801759722 0.0 38-39 3.0678563813713624E-4 0.0 0.0 0.019787673659845288 0.0 40-41 3.0678563813713624E-4 0.0 0.0 0.025386511555848024 0.0 42-43 3.0678563813713624E-4 0.0 0.0 0.035433741204839234 0.0 44-45 4.601784572057044E-4 0.0 0.0 0.04808864877799611 0.0 46-47 4.601784572057044E-4 0.0 0.0 0.05982319943674157 0.0 48-49 4.601784572057044E-4 0.0 0.0 0.07454891006732411 0.0 50-51 4.601784572057044E-4 0.0 0.0 0.09241917348881229 0.0 52-53 4.601784572057044E-4 0.0 0.0 0.12056675578789455 0.0 54-55 4.601784572057044E-4 0.0 0.0 0.14027773303820557 0.0 56-57 4.601784572057044E-4 0.0 0.0 0.18407138288228175 0.0 58-59 4.601784572057044E-4 0.0 0.0 0.24680904588132613 0.0 60-61 4.601784572057044E-4 0.0 0.0 0.30578858481319054 0.0 62-63 4.601784572057044E-4 0.0 0.0 0.39544668755876866 0.0 64-65 4.601784572057044E-4 0.0 0.0 0.46332300999661 0.0 66-67 4.601784572057044E-4 0.0 0.0 0.5680136090109078 0.0 68-69 4.601784572057044E-4 0.0 0.0 0.702002236467302 0.0 70-71 5.368748667399885E-4 0.0 0.0 0.8166633687210567 0.0 72-73 6.135712762742725E-4 0.0 0.0 0.9727405621233247 0.0 74-75 6.135712762742725E-4 0.0 0.0 1.1426998056512983 0.0 76-77 6.135712762742725E-4 0.0 0.0 1.2755379869646783 0.0 78-79 6.135712762742725E-4 0.0 0.0 1.4188068799747207 0.0 80-81 6.135712762742725E-4 0.0 0.0 1.649969858311053 0.0 82-83 6.135712762742725E-4 0.0 0.0 1.8479232913190402 0.0 84-85 6.902676858085565E-4 0.0 0.0 2.113829743174403 0.0 86-87 7.669640953428406E-4 0.0 0.0 2.464255638336547 7.669640953428406E-5 88-89 7.669640953428406E-4 0.0 0.0 2.893908924547606 1.5339281906856812E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 30 1.30002E-4 63.34106 3 TACGCTC 40 5.3832156E-4 47.505795 4 TACCTGG 575 0.0 42.13557 2 GTACCTG 615 0.0 39.40109 1 GGACCGA 125 5.646143E-9 34.198925 6 GGTATCA 4165 0.0 29.774105 1 GTATCAA 6065 0.0 29.76912 1 GCGACTG 65 0.0058431393 29.22985 9 AGGACCG 170 3.6307028E-9 27.944584 5 GTACATG 3345 0.0 25.283493 1 ACCTGGG 870 0.0 25.118004 3 TACATGG 3360 0.0 24.883986 2 TATCAAC 7345 0.0 24.318827 2 ATCAACG 7365 0.0 24.252787 3 TCAACGC 7430 0.0 24.040615 4 GTCCTAT 160 1.8530536E-6 23.756542 1 ACATGGG 3300 0.0 23.752897 3 CAACGCA 7540 0.0 23.689892 5 AACGCAG 7665 0.0 23.361952 6 ACCGAGT 170 3.1463787E-6 22.352238 8 >>END_MODULE