FastQCFastQC Report
Thu 26 May 2016
SRR937642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688420
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54190.787164812178612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.6147410011330292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.4215449870718457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.20626942854652686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12630.18346358327765028No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12090.17561953458644433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.14584120159205136No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8910.12942680340489815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.11606286859765841No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.10778303942360769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG5650.048.786862
GTACCTG7050.038.438581
AAGCCGT650.00583775229.2355866
GGACCGC650.00583775229.2355866
ACCTGGG8950.028.6722953
GTACATG40350.028.5136871
GTACACG1056.860002E-527.1671141
TACATGG41150.027.1407222
GCGGTCG350.00840625927.133564-65
GTATCAA60150.027.0316181
ACATGGG40550.026.6027623
GGTATCA42350.026.0445081
CATGGGA26200.024.6713334
CCTACAC2853.6379788E-1223.3439793
TATCAAC70050.023.338542
ATCAACG69700.022.9767633
TCAACGC69800.022.947194
ATGGGAT11650.022.84475
CAACGCA70550.022.8363175
GAGTACT38950.022.74599512-13
Produced by FastQC (version 0.11.5)