##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937642_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688420 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75092240202202 33.0 31.0 34.0 30.0 34.0 2 31.84640771621975 33.0 31.0 34.0 30.0 34.0 3 31.883250050841056 33.0 31.0 34.0 30.0 34.0 4 35.46218587490195 37.0 35.0 37.0 33.0 37.0 5 35.46138694401673 37.0 35.0 37.0 33.0 37.0 6 35.56631852648093 37.0 35.0 37.0 33.0 37.0 7 35.5116731065338 37.0 35.0 37.0 33.0 37.0 8 35.51394788065425 37.0 35.0 37.0 33.0 37.0 9 37.1191641730339 39.0 37.0 39.0 34.0 39.0 10-11 37.016890851515065 39.0 37.0 39.0 33.0 39.0 12-13 36.895441736149444 39.0 37.0 39.0 33.0 39.0 14-15 38.01052990906714 40.0 38.0 41.0 33.0 41.0 16-17 37.97183114958891 40.0 38.0 41.0 33.0 41.0 18-19 37.95864588477964 40.0 38.0 41.0 33.0 41.0 20-21 37.78298567734814 40.0 38.0 41.0 32.5 41.0 22-23 37.66728378025043 40.0 37.0 41.0 32.5 41.0 24-25 37.521690973533595 40.0 37.0 41.0 32.0 41.0 26-27 37.61479837889661 40.0 37.0 41.0 32.0 41.0 28-29 37.557607274628865 40.0 37.0 41.0 32.0 41.0 30-31 37.329148630196684 39.5 37.0 41.0 32.0 41.0 32-33 37.057090148455885 39.0 36.0 41.0 31.5 41.0 34-35 36.78910040382325 39.0 35.5 41.0 31.0 41.0 36-37 36.77786017256907 39.0 36.0 41.0 31.0 41.0 38-39 36.731467708666216 39.0 36.0 41.0 31.0 41.0 40-41 36.543034048981724 39.0 35.0 41.0 30.0 41.0 42-43 36.30570945062607 39.0 35.0 41.0 30.0 41.0 44-45 36.0409147032335 39.0 35.0 41.0 29.5 41.0 46-47 35.631201156270876 39.0 35.0 41.0 27.5 41.0 48-49 35.258417826326955 39.0 35.0 40.0 26.0 41.0 50-51 34.35613651549926 38.0 34.0 39.5 24.0 40.5 52-53 34.46994712530142 38.0 34.5 39.5 24.0 40.5 54-55 34.79016152929897 38.5 35.0 40.0 24.0 41.0 56-57 34.54674907759798 38.0 34.0 40.0 23.5 41.0 58-59 34.32939194096627 38.0 34.0 40.0 23.0 41.0 60-61 34.03500987769094 38.0 34.0 40.0 22.0 41.0 62-63 33.692166845820864 37.5 33.5 40.0 21.5 41.0 64-65 33.33858836175591 37.0 33.0 40.0 19.5 41.0 66-67 32.93235742715203 36.5 32.5 39.0 18.0 41.0 68-69 32.46434734609686 36.0 32.0 39.0 14.5 40.5 70-71 32.02580256238924 35.5 31.5 39.0 10.0 40.0 72-73 31.549620144679118 35.0 31.0 38.0 7.5 40.0 74-75 31.06311699253363 35.0 31.0 37.5 4.5 39.5 76-77 30.592548153743355 35.0 30.0 37.0 2.0 39.0 78-79 30.063122802940065 34.5 29.5 36.0 2.0 39.0 80-81 29.51605851079283 34.0 29.0 36.0 2.0 37.5 82-83 28.993960808808577 34.0 29.0 35.0 2.0 37.0 84-85 28.509069318148804 34.0 28.5 35.0 2.0 37.0 86-87 27.980158188315272 34.0 27.0 35.0 2.0 36.0 88-89 27.49009325702333 33.0 26.0 35.0 2.0 36.0 90-91 26.96067371662648 33.0 25.5 35.0 2.0 35.0 92-93 26.40189491879957 33.0 25.0 35.0 2.0 35.0 94-95 25.773856802533338 32.0 23.5 35.0 2.0 35.0 96-97 25.012872955463234 32.0 19.0 34.0 2.0 35.0 98-99 24.110190726591327 31.5 3.5 34.0 2.0 35.0 100-101 21.594809128148512 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1860921986855999 1101 2 0.2257700747512814 1101 3 0.22525717183158278 1101 4 0.06419289745506518 1101 5 0.07077924033187344 1101 6 0.052838781636666 1101 7 0.16702928187205401 1101 8 0.2618422673427787 1101 9 0.3204048682878664 1101 10-11 0.13883714116876433 1101 12-13 0.21631423541386852 1101 14-15 0.5040285174005987 1101 16-17 0.3852909333326906 1101 18-19 0.4183962927321119 1101 20-21 0.6391953333585718 1101 22-23 0.46091933956194 1101 24-25 0.3509458834818986 1101 26-27 0.3854709645810104 1101 28-29 0.3835037181260361 1101 30-31 0.553411700531349 1101 32-33 0.48134588076760565 1101 34-35 0.3128518440402175 1101 36-37 0.42754125648576036 1101 38-39 0.5821747461780049 1101 40-41 0.7456614377048325 1101 42-43 0.3910499728859733 1101 44-45 0.6107272696514201 1101 46-47 0.8549272580250857 1101 48-49 0.94239201030571 1101 50-51 0.5646000800754436 1101 52-53 0.7317981837809597 1101 54-55 1.0015634271831217 1101 56-57 1.0082993790191637 1101 58-59 1.0131189388347366 1101 60-61 1.0567666703544845 1101 62-63 1.0400219099195596 1101 64-65 1.0668693074124889 1101 66-67 1.225901691414336 1101 68-69 1.380175035615295 1101 70-71 1.372847104691953 1101 72-73 1.091778267212078 1101 74-75 1.0247479255345837 1101 76-77 1.1880034907858636 1101 78-79 1.324125767999142 1101 80-81 1.4486451179616502 1101 82-83 1.4350982761712707 1101 84-85 1.5573821127121477 1101 86-87 1.3964667640529207 1101 88-89 1.494036688443611 1101 90-91 1.5711805221310442 1101 92-93 1.4087567755448838 1101 94-95 1.7002631140670559 1101 96-97 1.8776641454438732 1101 98-99 2.0147141654037988 1101 100-101 2.296568737409274 1102 1 0.31148842478408056 1102 2 0.3024864951662565 1102 3 0.2576231177820638 1102 4 0.2378091475494557 1102 5 0.3212058808211893 1102 6 0.4621065709089862 1102 7 0.39573375467529814 1102 8 0.4810356357470553 1102 9 0.6754066721758747 1102 10-11 0.4846282089865568 1102 12-13 0.5346157280263171 1102 14-15 0.8024570372895141 1102 16-17 0.8408288695170043 1102 18-19 0.5397618779049935 1102 20-21 0.6095066928189112 1102 22-23 0.8755568419741167 1102 24-25 0.73525793093674 1102 26-27 0.86267401524929 1102 28-29 0.8848822241711574 1102 30-31 0.7263835850489571 1102 32-33 0.6725974434380362 1102 34-35 0.7881285101195701 1102 36-37 0.9108481607622352 1102 38-39 1.0717390442977717 1102 40-41 0.9938361547461128 1102 42-43 1.1446262857388874 1102 44-45 0.9382965515361832 1102 46-47 0.8330700084928253 1102 48-49 1.2093766646723694 1102 50-51 0.9351321011851326 1102 52-53 1.0001839976700637 1102 54-55 1.2430494952945779 1102 56-57 1.4478523504002752 1102 58-59 1.280904855083996 1102 60-61 1.103797806300527 1102 62-63 1.307749933949026 1102 64-65 1.5635912653441437 1102 66-67 1.489673747091551 1102 68-69 1.4477629436925241 1102 70-71 1.4703040421096212 1102 72-73 1.3984719502479805 1102 74-75 1.4026012149033882 1102 76-77 1.3033390559062177 1102 78-79 1.2959918272170086 1102 80-81 1.173203165399709 1102 82-83 0.9907603869719495 1102 84-85 1.2397963021791192 1102 86-87 1.5927566278803518 1102 88-89 1.5401214124487446 1102 90-91 1.6301877964143117 1102 92-93 2.0613271062017517 1102 94-95 2.111093993273677 1102 96-97 2.1663332117010974 1102 98-99 2.270713561311073 1102 100-101 1.986831098237218 1103 1 0.11556416290988736 1103 2 0.2587766106989875 1103 3 0.1572059162416224 1103 4 0.03447153522596125 1103 5 0.050345803799366706 1103 6 0.06852505614646986 1103 7 0.2053935749577107 1103 8 0.09428980776245766 1103 9 0.06375712146984114 1103 10-11 0.21657621925614023 1103 12-13 0.34706758938703075 1103 14-15 0.5084101823472835 1103 16-17 0.34841872831032816 1103 18-19 0.2849640601899708 1103 20-21 0.49644507536062576 1103 22-23 0.4107301410755255 1103 24-25 0.2610121029521437 1103 26-27 0.07722793052528232 1103 28-29 0.09526412404279228 1103 30-31 -0.01366776283294513 1103 32-33 0.06993377206447349 1103 34-35 0.0599192675008311 1103 36-37 0.06927242263643052 1103 38-39 0.11325059757119504 1103 40-41 0.1613907118706308 1103 42-43 0.3035800726451754 1103 44-45 0.0038774244501809108 1103 46-47 0.0654191740897545 1103 48-49 -0.018736300667804073 1103 50-51 -0.0537478731409351 1103 52-53 0.035100557361971596 1103 54-55 -0.04773997838963595 1103 56-57 0.2390269331987298 1103 58-59 0.29892475238823124 1103 60-61 0.2863074340283802 1103 62-63 0.4402756078899799 1103 64-65 0.4661426132260402 1103 66-67 0.47746257892723776 1103 68-69 0.519248823024995 1103 70-71 0.3477912051391492 1103 72-73 0.18021135974733937 1103 74-75 -0.1510733678950693 1103 76-77 -0.19035478234795633 1103 78-79 -0.16021805725025473 1103 80-81 0.33326414100946167 1103 82-83 0.2721467797832453 1103 84-85 0.37246639203791077 1103 86-87 0.21220463814993096 1103 88-89 0.2282472147594028 1103 90-91 0.4058296449380592 1103 92-93 0.5043149110797991 1103 94-95 0.5061196672146302 1103 96-97 0.8427613246664372 1103 98-99 0.8511692737629346 1103 100-101 1.4757913036287462 1104 1 -0.18100591396770227 1104 2 -0.15498743424483052 1104 3 -0.16803063018060982 1104 4 -0.3240589201258999 1104 5 -0.2562035752662766 1104 6 -0.17332887643780026 1104 7 -0.06546686265317447 1104 8 -0.09097039330200118 1104 9 -0.147789300765524 1104 10-11 -0.17413194159543366 1104 12-13 -0.27041466963130034 1104 14-15 -0.06591713876448324 1104 16-17 -0.40381367854951833 1104 18-19 -0.12813469794131294 1104 20-21 0.02304915578640987 1104 22-23 -0.04501350173937624 1104 24-25 -0.009316105208704073 1104 26-27 -0.12057662299334027 1104 28-29 -0.2582914643664367 1104 30-31 -0.3165211040511622 1104 32-33 -0.20311471995208308 1104 34-35 -0.05038528601779291 1104 36-37 -0.17907914448361595 1104 38-39 -0.13222545210354752 1104 40-41 -0.09266014561014657 1104 42-43 -0.34484541523182344 1104 44-45 -0.21916367549068383 1104 46-47 -0.35234398380498533 1104 48-49 -0.3170878677878548 1104 50-51 -0.46631440182218853 1104 52-53 -0.461454735364228 1104 54-55 -0.36794252126366445 1104 56-57 -0.20807537312888513 1104 58-59 -0.4182692156780021 1104 60-61 -0.20660356538025582 1104 62-63 -0.3660963613589914 1104 64-65 -0.41508890658471387 1104 66-67 -0.36331950268128566 1104 68-69 -0.17645638099628513 1104 70-71 -0.010650545304375925 1104 72-73 -0.013254045338410236 1104 74-75 -0.3134772709110365 1104 76-77 -0.4811738243349417 1104 78-79 -0.3144611086991027 1104 80-81 -0.026508183059135604 1104 82-83 0.07704033389324039 1104 84-85 0.307549183285694 1104 86-87 0.1780458563640046 1104 88-89 0.3531659019267579 1104 90-91 0.17754756332954358 1104 92-93 0.2625718596309561 1104 94-95 0.32060074350085443 1104 96-97 0.7739041395688666 1104 98-99 0.8380039869503939 1104 100-101 0.9301600203644043 1105 1 -0.003631460276245946 1105 2 0.003113421429244312 1105 3 0.07217767873279968 1105 4 0.048307914549830855 1105 5 -0.047779539364803725 1105 6 0.025902105326792935 1105 7 0.043853454163922834 1105 8 0.1333928746696671 1105 9 0.035184292228336744 1105 10-11 0.10446448500246674 1105 12-13 0.11199817217784158 1105 14-15 0.017896220467918056 1105 16-17 0.017585917893818248 1105 18-19 -0.013913302740455435 1105 20-21 0.22456688883619336 1105 22-23 0.05510224869181002 1105 24-25 0.11849602325961683 1105 26-27 0.06881452555234802 1105 28-29 0.06447641520288272 1105 30-31 -0.05006277420638838 1105 32-33 0.08975689544670473 1105 34-35 0.2600937909928689 1105 36-37 -0.05613251108385242 1105 38-39 0.2580492092270177 1105 40-41 0.21651072128313587 1105 42-43 -0.06300358097471559 1105 44-45 -0.19176242965602341 1105 46-47 -0.01823444580200828 1105 48-49 -0.03346686777110364 1105 50-51 -0.040102685283059714 1105 52-53 -0.1928153576117495 1105 54-55 -0.20953502113808753 1105 56-57 -0.15006828995602461 1105 58-59 0.04187223846557231 1105 60-61 -0.10108961672069938 1105 62-63 0.17791287355787233 1105 64-65 0.17822101153178949 1105 66-67 0.1476631828961743 1105 68-69 0.3138356533646345 1105 70-71 0.2639071750191384 1105 72-73 0.29435952823114064 1105 74-75 0.42812402013536754 1105 76-77 0.57893790727476 1105 78-79 0.4892456261758973 1105 80-81 0.725897876988757 1105 82-83 0.8548434618759586 1105 84-85 0.7925895389513968 1105 86-87 0.4359045361811482 1105 88-89 0.20953594328713265 1105 90-91 0.4077925439889647 1105 92-93 0.4521765080677973 1105 94-95 0.5231817897340463 1105 96-97 0.8064398484722268 1105 98-99 1.0294244310301686 1105 100-101 1.2021306243597856 1106 1 -0.01945281501880558 1106 2 -0.128320163494557 1106 3 0.11582221505316781 1106 4 -0.1407406888826941 1106 5 -0.16043518092049425 1106 6 -0.29437816965998564 1106 7 -0.14660133166368183 1106 8 -0.2056677474158093 1106 9 0.07763657817612568 1106 10-11 -0.2783091797251842 1106 12-13 -0.253356858830287 1106 14-15 -0.2748877803012775 1106 16-17 -0.052069387139589196 1106 18-19 -0.06119468344330414 1106 20-21 -0.11357190256806149 1106 22-23 -0.10629023243890856 1106 24-25 -0.45008721793070805 1106 26-27 -0.19465237507743183 1106 28-29 0.010438147811889564 1106 30-31 0.12690948674260483 1106 32-33 0.33695414557890757 1106 34-35 0.2094099296400529 1106 36-37 -0.012370191964585331 1106 38-39 0.29412920538104714 1106 40-41 0.12416660321188289 1106 42-43 0.10905545464857624 1106 44-45 0.030935998304165935 1106 46-47 0.4714463387756638 1106 48-49 0.9403468911827915 1106 50-51 0.606714775415945 1106 52-53 0.6903212704349784 1106 54-55 0.7706810742419421 1106 56-57 0.8151304985637609 1106 58-59 0.7746675367685327 1106 60-61 0.6407641403102176 1106 62-63 0.9893630442227419 1106 64-65 1.1378106947201019 1106 66-67 1.308808079776135 1106 68-69 1.0401528977278858 1106 70-71 1.0555373851051932 1106 72-73 1.0313998147558294 1106 74-75 1.0882942042611283 1106 76-77 0.9242674587386333 1106 78-79 0.8435807542947344 1106 80-81 0.568005227596899 1106 82-83 0.645661211022631 1106 84-85 0.49118455842161524 1106 86-87 0.5357615142110497 1106 88-89 0.8400044303790963 1106 90-91 0.9141912748192169 1106 92-93 0.7847266258505989 1106 94-95 0.9044218743453598 1106 96-97 0.924021935871874 1106 98-99 0.9783764044929839 1106 100-101 0.7015128655981862 1107 1 0.4075441127845707 1107 2 0.3794115313339077 1107 3 0.38928611674287694 1107 4 0.39136376580240295 1107 5 0.5310266726381343 1107 6 0.5518583894798041 1107 7 0.49994244713816016 1107 8 0.5040224727582796 1107 9 0.40506873383408504 1107 10-11 0.4887671131575573 1107 12-13 0.6289076060954741 1107 14-15 0.7274995724893003 1107 16-17 0.5422470491123264 1107 18-19 0.6506908772325772 1107 20-21 0.7610440728543608 1107 22-23 0.8906695729886422 1107 24-25 0.8313963970206473 1107 26-27 0.9235311886706512 1107 28-29 0.8828267806843897 1107 30-31 1.0123869573007198 1107 32-33 1.0636054512624753 1107 34-35 0.9629685573922231 1107 36-37 1.027459557139359 1107 38-39 0.8566612074291768 1107 40-41 1.036954621645073 1107 42-43 1.1247684602809258 1107 44-45 0.8237291370650439 1107 46-47 0.9958703019093065 1107 48-49 0.8497682941525753 1107 50-51 1.0471397626151315 1107 52-53 0.9403532724831081 1107 54-55 1.0506413917022641 1107 56-57 1.1319571754710793 1107 58-59 1.0851194056881468 1107 60-61 1.2927610681135917 1107 62-63 0.9957058501623237 1107 64-65 1.259469680895215 1107 66-67 1.321583297390056 1107 68-69 1.4832212541026948 1107 70-71 1.4563438367180943 1107 72-73 1.2979640748684744 1107 74-75 1.2013702411274885 1107 76-77 1.2871514833162045 1107 78-79 1.1654189519393867 1107 80-81 1.2681012337412945 1107 82-83 1.5104717588976975 1107 84-85 1.5933414964656478 1107 86-87 1.7560956155935408 1107 88-89 1.5652668471737705 1107 90-91 1.9715773475278695 1107 92-93 2.0966185869361063 1107 94-95 1.8060987816674299 1107 96-97 2.266424649645554 1107 98-99 2.1540932503711225 1107 100-101 1.6630720053831354 1108 1 0.28562824799305986 1108 2 -0.0019315067448779644 1108 3 0.1189616657189454 1108 4 0.0239815797673657 1108 5 0.1172488126167508 1108 6 0.010839391206914684 1108 7 0.11078626653119983 1108 8 0.06176776431185971 1108 9 -0.11404262400784404 1108 10-11 0.034587081904987826 1108 12-13 0.13144173719177843 1108 14-15 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-1.0386288644862525 2316 78-79 -0.8250849766886503 2316 80-81 -0.6274264138369965 2316 82-83 -0.4733070831193764 2316 84-85 -0.582361297728589 2316 86-87 -0.4598841072513764 2316 88-89 -0.46730425306851586 2316 90-91 -0.41331781357984454 2316 92-93 -0.8517431638507524 2316 94-95 -1.0044836063044045 2316 96-97 -0.9413581662863351 2316 98-99 -0.774679138280014 2316 100-101 -0.28563222666631916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2890.0 3 544.0 4 517.0 5 555.0 6 651.0 7 815.0 8 887.0 9 1148.0 10 1422.0 11 1917.0 12 2682.0 13 3469.0 14 3959.0 15 4786.0 16 6197.0 17 8529.0 18 7424.0 19 4810.0 20 4670.0 21 4876.0 22 5334.0 23 5896.0 24 6467.0 25 7343.0 26 8577.0 27 9970.0 28 11681.0 29 13894.0 30 16857.0 31 21018.0 32 26819.0 33 34691.0 34 47137.0 35 67978.0 36 94239.0 37 130017.0 38 104876.0 39 12875.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68554728191112 19.621698089841967 14.545523417683711 21.1472312105632 2 14.65698524486075 21.116800354169943 42.13486418631203 22.091350214657275 3 19.216867820778678 27.064790652158717 30.058365758754864 23.659975768307742 4 11.836322171186573 17.374385800374565 38.38212464283946 32.40716738559941 5 12.29715067974456 38.85415528904617 34.400535931435726 14.44815809977354 6 29.192234694115182 37.53624158729419 18.738065019445287 14.533458699145338 7 27.06276150627615 34.45675823176278 21.24504275059123 17.235437511369838 8 25.885315092417404 35.63760733517683 20.091689710376947 18.385387862028818 9 26.751842666014603 17.627443788498038 21.09095725480607 34.52975629068129 10-11 24.147554643466616 28.172559464681417 27.406914167821938 20.272971724030025 12-13 25.588322191997744 25.80037870352986 28.306560652965224 20.304738451507173 14-15 22.31605957819037 26.718927726476522 27.61506032557398 23.349952369759126 16-17 20.40079924092486 30.421871725615297 28.078722029478193 21.098607003981652 18-19 20.233508337244544 28.52003449141535 31.366667758167154 19.879789413172954 20-21 21.49334831212147 27.404051839800697 31.351408772486373 19.75119107559146 22-23 20.94400882493335 27.555868929947724 31.285945886160686 20.214176358958238 24-25 20.675917571382833 27.623773903425793 31.69448729516547 20.005821230025905 26-27 20.555343323983017 27.746937498408265 31.6648293476244 20.032889829984317 28-29 20.12432747525131 28.046528924477098 31.809392677312566 20.01975092295903 30-31 20.445900522718194 27.81809727084778 31.640787883242382 20.095214323191644 32-33 20.149629020461184 28.1437195474741 31.444145655409315 20.2625057766554 34-35 20.63832589725919 27.996073228895966 31.344380591155158 20.02122028268968 36-37 20.582786776700754 27.83757117848136 31.458451266842356 20.121190777975524 38-39 20.4053467119962 28.190279274252163 31.524007895079542 19.880366118672097 40-41 20.434570365784367 28.153297835732783 31.449714925506694 19.96241687297616 42-43 20.330830109410847 28.221115121863903 31.04097104035985 20.4070837283654 44-45 20.411699004223955 28.425349540313107 30.714433841105144 20.448517614357804 46-47 20.591135083847583 28.696631617090105 30.260947262647715 20.451286036414594 48-49 20.585720284432828 28.784305106676868 30.07183595407331 20.558138654816997 50-51 20.58758247891953 29.050772883965887 29.97469688292087 20.38694775419372 52-53 20.634885975561826 29.317585645701776 29.704800002329108 20.34272837640729 54-55 20.447402907835116 29.1011993855723 29.76867277359096 20.682724933001623 56-57 20.614385123804606 29.497906579995565 29.432503119213994 20.45520517698584 58-59 20.768184265281477 29.183290409882577 29.6153622302734 20.433163094562545 60-61 20.756660497922415 29.141715260076584 29.940378504836062 20.161245737164936 62-63 20.656466858382906 29.278896203334643 29.94628986884194 20.118347069440514 64-65 20.535659724546125 29.18607831782826 29.923818059601164 20.35444389802445 66-67 20.518955425615033 29.26389775985963 29.664509990208387 20.552636824316952 68-69 20.30778385873428 29.434286749391276 29.72819021665196 20.529739175222485 70-71 20.54687687558791 29.491426039725905 29.470037517214486 20.491659567471697 72-73 20.492827721268327 29.379353090867294 29.340068878319336 20.787750309545043 74-75 20.316162914047982 29.699334554698787 29.330087353337923 20.654415177915304 76-77 20.365736985125338 29.54466582028905 29.34306303815611 20.7465341564295 78-79 20.38466995288939 29.47399821743638 29.308765483747752 20.83256634592648 80-81 20.35773968930222 29.78431000226261 29.22716964989207 20.630780658543095 82-83 20.566563377413114 29.80360494042683 29.18706993118899 20.442761750971066 84-85 20.500790668511318 29.555581741449217 29.114422171887117 20.829205418152352 86-87 20.386349451595024 29.59539220521505 29.172322388511358 20.845935954678573 88-89 20.553974141762733 29.2692170874165 29.169555282839475 21.007253487981295 90-91 20.518952172328323 29.297167995960827 29.328596714971344 20.855283116739503 92-93 20.617759494269432 29.409847633388985 29.150435203270664 20.821957669070922 94-95 20.302671197350264 29.422381489669565 29.13190269069355 21.143044622286627 96-97 20.232583517634733 29.285589570480735 29.24223024094983 21.2395966709347 98-99 20.39425913072075 29.488528301996613 28.882529870651457 21.234682696631175 100-101 20.54232888909581 29.076414309242114 29.35102686911129 21.03022993255079 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1395.0 1 879.5 2 827.0 3 1127.0 4 769.5 5 637.0 6 949.0 7 1698.0 8 2575.5 9 2887.0 10 2341.5 11 1800.0 12 1776.0 13 1862.5 14 1923.0 15 1908.0 16 1860.0 17 1868.5 18 1972.0 19 2082.0 20 2197.5 21 2441.0 22 2810.5 23 3286.0 24 3888.0 25 4665.5 26 5696.5 27 6938.0 28 7890.5 29 8972.5 30 10411.5 31 11617.0 32 12919.0 33 14655.5 34 16675.0 35 18239.0 36 20129.5 37 21789.0 38 22411.0 39 22815.5 40 23168.0 41 23762.0 42 24013.0 43 24620.0 44 25571.5 45 26184.5 46 25978.5 47 26166.5 48 28516.0 49 27704.5 50 24131.0 51 21426.5 52 18936.5 53 17446.5 54 16040.5 55 14272.5 56 12378.5 57 10721.5 58 9303.0 59 8032.0 60 6767.5 61 5371.0 62 4101.0 63 2982.5 64 2122.5 65 1510.5 66 1097.5 67 853.5 68 640.0 69 449.0 70 326.5 71 232.5 72 171.5 73 143.0 74 113.0 75 90.5 76 63.5 77 34.5 78 26.0 79 20.5 80 12.5 81 9.0 82 5.0 83 2.5 84 1.5 85 1.5 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 1.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2958949478516022 2 0.25333372069376253 3 0.24926643618721128 4 0.2547863223032451 5 0.25638418407367597 6 0.19813485953342436 7 0.18811190842799452 8 0.19174341245170098 9 0.19929694082101043 10-11 0.19646436768251938 12-13 0.1945033555097179 14-15 0.19704540832631243 16-17 0.18418988408239156 18-19 0.18774875802562388 20-21 0.17808895732256472 22-23 0.1853519653699776 24-25 0.18593300601377066 26-27 0.18556985561140002 28-29 0.19806222945295024 30-31 0.19443072542924378 32-33 0.2015484733157084 34-35 0.19486650591208854 36-37 0.17997733941489208 38-39 0.16799337613666077 40-41 0.1661049940443334 42-43 0.1800499694953662 44-45 0.18411725400191745 46-47 0.18164783126579703 48-49 0.19878853025769153 50-51 0.19617384736062288 52-53 0.21237035530635365 54-55 0.18542459545045176 56-57 0.16668603468812646 58-59 0.1670491850904971 60-61 0.15746201446791203 62-63 0.1626187501815752 64-65 0.1624008599401528 66-67 0.1593503965602394 68-69 0.1626187501815752 70-71 0.16494291275674733 72-73 0.16283664042299759 74-75 0.17591005490834083 76-77 0.17104383951657418 78-79 0.17540164434502195 80-81 0.1685744167804538 82-83 0.14961796577670608 84-85 0.14918218529386132 86-87 0.15441155108799862 88-89 0.159132506318817 90-91 0.1671218151709712 92-93 0.1594230266407135 94-95 0.16073036808924784 96-97 0.1655965834810145 98-99 0.1753290142645478 100-101 0.15804305511170508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.10104269615532 #Duplication Level Percentage of deduplicated Percentage of total 1 85.76757359433942 58.40861191293754 2 8.906440688266702 12.130757951648514 3 2.2297606600714817 4.555470777412063 4 0.9420763698129749 2.5662553233468963 5 0.4804814917805732 1.6360645293230607 6 0.33146061611896466 1.354368814224694 7 0.2303308204009847 1.098003832405756 8 0.15804949827133485 0.8610668503905681 9 0.13601604691157662 0.833655115627879 >10 0.7563896309182437 9.446608596195867 >50 0.041945886691599266 1.9698557893044686 >100 0.016692596928557284 1.9771284168950292 >500 0.0012840459173597582 0.619109650160941 >1k 0.0012840459173597582 1.7532695601309136 >5k 2.1400765289329306E-4 0.7897728799957956 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5419 0.787164812178612 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4232 0.6147410011330292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.4215449870718457 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.20626942854652686 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1263 0.18346358327765028 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1209 0.17561953458644433 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.14584120159205136 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 891 0.12942680340489815 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.11606286859765841 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 742 0.10778303942360769 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4526016094825833E-4 0.0 10-11 0.0 0.0 0.0 1.4526016094825833E-4 0.0 12-13 0.0 0.0 0.0 1.4526016094825833E-4 0.0 14-15 0.0 0.0 0.0 2.178902414223875E-4 0.0 16-17 0.0 0.0 0.0 2.9052032189651666E-4 0.0 18-19 0.0 0.0 0.0 3.631504023706458E-4 0.0 20-21 0.0 0.0 0.0 5.810406437930333E-4 0.0 22-23 0.0 0.0 0.0 7.263008047412917E-4 0.0 24-25 2.9052032189651666E-4 0.0 0.0 0.0010168211266378083 0.0 26-27 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.0017431219313791 0.0 28-29 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.002759943058016908 0.0 30-31 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.005447256035559688 0.0 32-33 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.008570349495947243 0.0 34-35 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.011838703117283054 0.0 36-37 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.014889166497196477 0.0 38-39 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.020336422532756165 0.0 40-41 2.9052032189651666E-4 1.4526016094825833E-4 0.0 0.02585630864878998 0.0 42-43 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.035516109351849165 0.0 44-45 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.048226373434821765 0.0 46-47 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.06028296679352721 0.0 48-49 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.07662473490020627 0.0 50-51 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.09746956799628134 0.0 52-53 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.1317509659800703 0.0 54-55 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.15121582754713692 0.0 56-57 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.19428546526829552 0.0 58-59 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.25260741988902125 0.0 60-61 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.3134714273263415 0.0 62-63 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.4044769181604253 0.0 64-65 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.4695534702652451 0.0 66-67 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.5784259608959648 0.0 68-69 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.7127189796926294 0.0 70-71 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.8197030882310218 0.0 72-73 4.35780482844775E-4 1.4526016094825833E-4 0.0 0.9831933993782865 0.0 74-75 4.35780482844775E-4 1.4526016094825833E-4 0.0 1.1524941169634815 0.0 76-77 4.35780482844775E-4 1.4526016094825833E-4 0.0 1.2785799366665698 0.0 78-79 4.35780482844775E-4 1.4526016094825833E-4 0.0 1.4157055286017257 0.0 80-81 4.35780482844775E-4 1.4526016094825833E-4 0.0 1.639551436622992 0.0 82-83 4.35780482844775E-4 1.4526016094825833E-4 0.0 1.8270096743267192 0.0 84-85 4.35780482844775E-4 1.4526016094825833E-4 0.0 2.0737340576973358 0.0 86-87 4.35780482844775E-4 1.4526016094825833E-4 0.0 2.3951221637953575 0.0 88-89 4.35780482844775E-4 1.4526016094825833E-4 0.0 2.8184829028790563 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 565 0.0 48.78686 2 GTACCTG 705 0.0 38.43858 1 AAGCCGT 65 0.005837752 29.235586 6 GGACCGC 65 0.005837752 29.235586 6 ACCTGGG 895 0.0 28.672295 3 GTACATG 4035 0.0 28.513687 1 GTACACG 105 6.860002E-5 27.167114 1 TACATGG 4115 0.0 27.140722 2 GCGGTCG 35 0.008406259 27.1335 64-65 GTATCAA 6015 0.0 27.031618 1 ACATGGG 4055 0.0 26.602762 3 GGTATCA 4235 0.0 26.044508 1 CATGGGA 2620 0.0 24.671333 4 CCTACAC 285 3.6379788E-12 23.343979 3 TATCAAC 7005 0.0 23.33854 2 ATCAACG 6970 0.0 22.976763 3 TCAACGC 6980 0.0 22.94719 4 ATGGGAT 1165 0.0 22.8447 5 CAACGCA 7055 0.0 22.836317 5 GAGTACT 3895 0.0 22.745995 12-13 >>END_MODULE