FastQCFastQC Report
Thu 26 May 2016
SRR937642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688420
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75391.0951163533889194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64030.930100810551698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.693326748206037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.3586473373812498No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.2129513959501467No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.18985503035937365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12820.1862235263356672No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12800.18593300601377066No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10870.15789779495075681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG405.3768425E-447.5175861
TGGATCG405.3806964E-447.5106775
TATCACG751.3593308E-744.3433042
GGATCGC459.625496E-442.2194446
GTACCTG7600.040.014811
TACCTGG7750.039.2346272
CTAGCGC550.002576067634.5532233
GGACCGA851.6319897E-533.5272066
ACCTGGG9500.031.5070823
GGTATCA47650.030.8141331
TACACCG650.005836050529.237345
ATCACGC1153.8548515E-628.9195463
GTATCAA68250.028.6149851
ACTATAC1501.0570038E-625.3390313
GAGTACT51050.023.77515212-13
GTACATG39050.023.6066861
TCAACGC82150.023.5384624
TATCAAC82550.023.481962
ATCAACG82350.023.4812953
CAACGCA83250.023.2845125
Produced by FastQC (version 0.11.5)