##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937642_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688420 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.264768600563606 33.0 31.0 34.0 31.0 34.0 2 32.45374771215246 34.0 31.0 34.0 31.0 34.0 3 32.52955608494814 34.0 31.0 34.0 31.0 34.0 4 36.14925191017112 37.0 35.0 37.0 35.0 37.0 5 36.08248307719125 37.0 35.0 37.0 35.0 37.0 6 36.13442520554313 37.0 35.0 37.0 35.0 37.0 7 36.08844745939979 37.0 35.0 37.0 35.0 37.0 8 36.07715202928445 37.0 35.0 37.0 35.0 37.0 9 37.757640684465876 39.0 37.0 39.0 35.0 39.0 10-11 37.69624865634351 39.0 37.0 39.0 35.0 39.0 12-13 37.59780221376485 39.0 37.0 39.0 35.0 39.0 14-15 38.78383399668807 40.0 38.0 41.0 35.0 41.0 16-17 38.75635948984632 40.0 38.0 41.0 35.0 41.0 18-19 38.80189709770198 40.0 38.0 41.0 35.0 41.0 20-21 38.72596162226547 40.0 38.0 41.0 34.5 41.0 22-23 38.56160047645332 40.0 38.0 41.0 34.0 41.0 24-25 38.61448461694896 40.0 38.0 41.0 34.0 41.0 26-27 38.476443159699016 40.0 38.0 41.0 34.0 41.0 28-29 38.324901223090556 40.0 38.0 41.0 34.0 41.0 30-31 38.28260872723047 40.0 37.5 41.0 34.0 41.0 32-33 38.12310217599721 40.0 37.0 41.0 34.0 41.0 34-35 37.94740928502949 40.0 37.0 41.0 33.5 41.0 36-37 37.75423433369164 40.0 37.0 41.0 33.0 41.0 38-39 37.60714098951222 40.0 36.5 41.0 33.0 41.0 40-41 37.40799729816101 40.0 36.0 41.0 33.0 41.0 42-43 37.14885607623253 40.0 36.0 41.0 32.5 41.0 44-45 36.76797521861654 40.0 36.0 41.0 31.5 41.0 46-47 36.29591528427413 39.5 35.5 41.0 30.5 41.0 48-49 35.85587867871358 39.0 35.0 41.0 28.5 41.0 50-51 35.579081810522645 39.0 35.0 41.0 27.5 41.0 52-53 35.331953603904594 39.0 35.0 40.5 27.0 41.0 54-55 35.12334112896197 39.0 35.0 40.0 26.0 41.0 56-57 34.87707431509834 38.5 35.0 40.0 26.0 41.0 58-59 34.62268237413207 38.0 34.5 40.0 25.0 41.0 60-61 34.29416780453793 38.0 34.0 40.0 24.0 41.0 62-63 33.960614159960485 38.0 34.0 40.0 23.0 41.0 64-65 33.58416518985503 37.0 33.5 40.0 22.0 41.0 66-67 33.15375134365649 36.5 33.0 39.0 20.5 41.0 68-69 32.724790099067434 36.0 32.5 39.0 19.0 40.0 70-71 32.315661224252636 35.5 32.0 39.0 18.0 40.0 72-73 31.81222727404782 35.0 31.5 38.0 14.0 40.0 74-75 31.31163751779437 35.0 31.0 37.0 9.5 39.0 76-77 30.053999012230904 33.5 29.5 36.0 8.0 38.5 78-79 30.641054879288806 34.0 31.0 36.0 7.0 39.0 80-81 30.414491153656197 34.0 31.0 36.0 3.5 38.0 82-83 30.040135382470005 34.0 30.0 35.5 2.0 37.0 84-85 29.633377153481888 34.0 30.0 35.0 2.0 37.0 86-87 29.19085006246187 34.0 29.5 35.0 2.0 36.0 88-89 28.7986672380233 34.0 29.0 35.0 2.0 36.0 90-91 28.262218558438164 34.0 29.0 35.0 2.0 35.5 92-93 27.780851079282996 33.5 27.5 35.0 2.0 35.0 94-95 27.30107710409343 33.0 26.5 35.0 2.0 35.0 96-97 26.752609598791437 33.0 25.5 35.0 2.0 35.0 98-99 25.87378489875367 32.5 24.5 34.5 2.0 35.0 100-101 23.262421922663492 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3037128196050176 1101 2 0.3461089805430788 1101 3 0.23543957988233188 1101 4 0.07391384492200359 1101 5 0.08244703656609431 1101 6 0.15900300040203774 1101 7 0.15739180277833498 1101 8 0.19797163321508293 1101 9 0.2852393303211116 1101 10-11 0.30542964592906685 1101 12-13 0.34797729604803607 1101 14-15 0.5081763325594153 1101 16-17 0.45098698909489343 1101 18-19 0.3352199277357215 1101 20-21 0.34059785382254404 1101 22-23 0.19514576429626374 1101 24-25 0.23216924567891084 1101 26-27 0.20522355788572355 1101 28-29 0.37362217589729596 1101 30-31 0.2767579773778053 1101 32-33 0.21863640740992452 1101 34-35 0.2694239575037116 1101 36-37 0.33515274307694654 1101 38-39 0.3229361668932995 1101 40-41 0.1676725561817065 1101 42-43 0.45141254589513835 1101 44-45 0.6850120206828194 1101 46-47 0.596099083880766 1101 48-49 0.6978245324324419 1101 50-51 0.5565462939660222 1101 52-53 0.6189979757941657 1101 54-55 0.5246129928008898 1101 56-57 0.6629477001254571 1101 58-59 0.5611909575869802 1101 60-61 0.6998347933341904 1101 62-63 0.37462539171808373 1101 64-65 0.496609028847935 1101 66-67 0.7865213134544362 1101 68-69 0.8752522348163083 1101 70-71 0.8074019507628023 1101 72-73 0.5981181528028046 1101 74-75 0.6704492680987535 1101 76-77 0.45310937843415644 1101 78-79 0.6738743123911632 1101 80-81 0.8093746427804085 1101 82-83 0.8303523476217443 1101 84-85 0.9372008809917034 1101 86-87 0.7458284947205804 1101 88-89 0.8548909747944151 1101 90-91 1.069446524629388 1101 92-93 0.9925394266169256 1101 94-95 0.9343355989689321 1101 96-97 1.2484848945949736 1101 98-99 1.1730295622598312 1101 100-101 1.4964580125657534 1102 1 0.26969065275796567 1102 2 0.26598950442571834 1102 3 0.31099983393689 1102 4 0.11900601101393704 1102 5 0.08635182483997994 1102 6 0.24803787836691527 1102 7 0.2137788248528878 1102 8 0.20391859051747474 1102 9 0.40324548871086563 1102 10-11 0.2726878200587919 1102 12-13 0.28738595638850484 1102 14-15 0.36177026478356566 1102 16-17 0.44222680551782645 1102 18-19 0.31990869412802425 1102 20-21 0.4049077198314208 1102 22-23 0.32393246083196203 1102 24-25 0.21192123205326396 1102 26-27 0.34120818708185396 1102 28-29 0.21003554312694206 1102 30-31 0.3920675329501435 1102 32-33 0.4018661220600066 1102 34-35 0.42635466303367764 1102 36-37 0.524411638761876 1102 38-39 0.376398373341992 1102 40-41 0.4369082949787213 1102 42-43 0.6554691963687702 1102 44-45 0.5855850697569593 1102 46-47 0.5056194174741862 1102 48-49 0.502518836341423 1102 50-51 0.26649284853992583 1102 52-53 0.5467400953206223 1102 54-55 0.600648130861245 1102 56-57 0.3761278288307608 1102 58-59 0.37479982432579817 1102 60-61 0.4095086671627115 1102 62-63 0.6773958280911856 1102 64-65 0.5752275023391746 1102 66-67 0.3909949557140848 1102 68-69 0.4450215888566049 1102 70-71 0.20433785820237205 1102 72-73 0.23668024430607204 1102 74-75 0.4991075949297006 1102 76-77 0.3755673646239259 1102 78-79 0.6182466013152919 1102 80-81 0.5170071865981036 1102 82-83 0.7104374240143052 1102 84-85 0.7526455184102154 1102 86-87 0.8094621124809116 1102 88-89 0.6408684723402871 1102 90-91 0.7068935616384593 1102 92-93 1.07474470068205 1102 94-95 1.3938815226015429 1102 96-97 1.513810811012032 1102 98-99 1.412246926569697 1102 100-101 1.3882146638783972 1103 1 0.21513352823934895 1103 2 0.17600234139619175 1103 3 0.06651973124112232 1103 4 0.09368508675206755 1103 5 -0.01794856026913294 1103 6 0.10018807092146886 1103 7 0.12199448595686846 1103 8 0.14165928371387793 1103 9 0.2181684668880166 1103 10-11 0.1584869214708604 1103 12-13 0.20394564830120743 1103 14-15 0.21247629815969304 1103 16-17 0.18168444351525892 1103 18-19 0.09181177500094151 1103 20-21 0.09670810494053939 1103 22-23 0.2690062734121952 1103 24-25 0.297993119087927 1103 26-27 0.22395850800612038 1103 28-29 0.02805544043117436 1103 30-31 0.18078704514526578 1103 32-33 0.26932440190596907 1103 34-35 0.019165959567693847 1103 36-37 0.08673513041784275 1103 38-39 0.36163585729577363 1103 40-41 0.39082357097358766 1103 42-43 -0.0700442824502332 1103 44-45 -0.0690041471878402 1103 46-47 0.02517975123542726 1103 48-49 0.02989367587929337 1103 50-51 -0.016690720137859216 1103 52-53 -0.09496401250992648 1103 54-55 -0.18752581008868674 1103 56-57 -0.17350310826807913 1103 58-59 -0.39614048376149213 1103 60-61 -0.45737194901187195 1103 62-63 -0.2934000640782699 1103 64-65 -0.08748109843104146 1103 66-67 -0.15844342682763823 1103 68-69 -0.23957404657343773 1103 70-71 -0.3817019364060954 1103 72-73 -0.44884607148340194 1103 74-75 0.23904661931994653 1103 76-77 -0.001117487750910584 1103 78-79 0.010544419030310337 1103 80-81 -0.1741994886265381 1103 82-83 -0.08707541295617105 1103 84-85 -0.22954318505576765 1103 86-87 0.18019381979798155 1103 88-89 0.30659376117212034 1103 90-91 0.45639612903255156 1103 92-93 0.30163815382710624 1103 94-95 0.2629604699699648 1103 96-97 0.43284161974117197 1103 98-99 0.2654401229753489 1103 100-101 0.23880837376286124 1104 1 0.1736639835912115 1104 2 0.1528799286226814 1104 3 0.08850376435762541 1104 4 -0.06670817167489673 1104 5 -0.0532087613335932 1104 6 0.09831048369498063 1104 7 0.020649128180409093 1104 8 0.043122914701463344 1104 9 0.07354989799387113 1104 10-11 -0.03076500046881847 1104 12-13 0.03980460749695425 1104 14-15 0.18576133659848182 1104 16-17 0.13539526551408443 1104 18-19 -0.152274564443168 1104 20-21 -0.11582885543793964 1104 22-23 0.040679839349515134 1104 24-25 -0.032329175412364464 1104 26-27 0.1410785553154028 1104 28-29 -0.13801493212943683 1104 30-31 0.2257160812186001 1104 32-33 0.025016300191005314 1104 34-35 -0.022680580940885875 1104 36-37 0.034096159394785275 1104 38-39 0.17443154032357455 1104 40-41 0.1009654988269304 1104 42-43 0.09332798248176033 1104 44-45 -0.16941810342675012 1104 46-47 -0.43343201694908373 1104 48-49 -0.6706459456464344 1104 50-51 -0.5458376746026801 1104 52-53 -0.3886955323206891 1104 54-55 -0.2248114398935357 1104 56-57 -0.2705462779901353 1104 58-59 -0.1381940023895183 1104 60-61 -0.18788643748023048 1104 62-63 -0.2899997092586375 1104 64-65 -0.05329274834234354 1104 66-67 -0.07181569175963176 1104 68-69 -0.334776589447479 1104 70-71 -0.17156000855275266 1104 72-73 -0.03860065457033102 1104 74-75 -0.30129193774687835 1104 76-77 0.08337393744128008 1104 78-79 0.013782148184660059 1104 80-81 0.1063223919175158 1104 82-83 0.08541128130757869 1104 84-85 0.14704611121862854 1104 86-87 -0.05831889457221351 1104 88-89 -0.0721909815836419 1104 90-91 -0.007427052516824517 1104 92-93 -0.10226486213977637 1104 94-95 -0.015380748244108844 1104 96-97 0.20098177349634838 1104 98-99 0.1350512997937976 1104 100-101 0.560467155548789 1105 1 0.153756165308927 1105 2 0.14479205039297227 1105 3 0.05671111880093349 1105 4 -0.08362489834480158 1105 5 -0.0052612906793640946 1105 6 2.1356311693665475E-4 1105 7 0.030183363515895678 1105 8 -0.030628738876124828 1105 9 0.17866929048202707 1105 10-11 0.048622422294457124 1105 12-13 0.04585481764782884 1105 14-15 -0.032303684584057635 1105 16-17 0.0524850945467179 1105 18-19 -0.0879033006912664 1105 20-21 0.05476069730072197 1105 22-23 0.05255106123869524 1105 24-25 0.19668713430480267 1105 26-27 -0.16380033582476727 1105 28-29 0.01273168954875814 1105 30-31 0.0012994382741382537 1105 32-33 0.039992689614805954 1105 34-35 0.012514457489096742 1105 36-37 0.03457025474131825 1105 38-39 -0.012471915862903415 1105 40-41 -0.09494786207998374 1105 42-43 0.17216491042763948 1105 44-45 -0.0733829219937121 1105 46-47 -0.09003418711267841 1105 48-49 -0.278175584454857 1105 50-51 -0.5062114668630926 1105 52-53 -0.3213729465439741 1105 54-55 -0.13163024965257364 1105 56-57 -0.3449645954435425 1105 58-59 -0.5341169917228967 1105 60-61 -0.1473633208841676 1105 62-63 -0.06210741367431183 1105 64-65 0.007369478084754633 1105 66-67 0.01803217910223509 1105 68-69 -0.278455527473902 1105 70-71 0.009762099988911643 1105 72-73 -0.04051796731837243 1105 74-75 0.26675173344654013 1105 76-77 -0.04315509854391664 1105 78-79 0.06916862485037711 1105 80-81 -0.013288828966960153 1105 82-83 -0.236723424564925 1105 84-85 -0.22202298739320625 1105 86-87 -0.08828213910075533 1105 88-89 -0.30169708516876526 1105 90-91 -0.22602896313153664 1105 92-93 -0.2733285102249745 1105 94-95 -0.15569099288986976 1105 96-97 -0.1552574468522856 1105 98-99 -0.2019896754402133 1105 100-101 -0.20726484766626996 1106 1 0.07744252654156014 1106 2 0.05231813086987813 1106 3 0.09674044431412909 1106 4 -0.09099233260277373 1106 5 -0.08161579286675646 1106 6 -0.0027406217780168163 1106 7 -0.12189345971376042 1106 8 -0.08126092002126484 1106 9 -0.021602331074632275 1106 10-11 -0.26653430094000896 1106 12-13 -0.06808321417756247 1106 14-15 0.022323496801455178 1106 16-17 0.13174811074785708 1106 18-19 0.10180753051876934 1106 20-21 -0.04219257417661737 1106 22-23 -0.12761936326013057 1106 24-25 -0.06822080281359888 1106 26-27 -0.07635982815686759 1106 28-29 0.1804111280372922 1106 30-31 0.08288806382098102 1106 32-33 -0.04077746566618856 1106 34-35 -0.17926358202823423 1106 36-37 -0.12714432628843753 1106 38-39 -0.12151354847672025 1106 40-41 -0.4037010470230129 1106 42-43 -0.4952140617371583 1106 44-45 -0.2900143339450594 1106 46-47 0.1056763556496847 1106 48-49 0.22734698657538388 1106 50-51 0.2700240506600977 1106 52-53 0.08960729479057505 1106 54-55 -0.012547032499554689 1106 56-57 -0.013464907928522507 1106 58-59 -0.14378479615537998 1106 60-61 0.046872533161305796 1106 62-63 0.058849511473518135 1106 64-65 0.10088902041445635 1106 66-67 0.26390377181412816 1106 68-69 0.042556683477499746 1106 70-71 0.3068379617263304 1106 72-73 0.48587379269316955 1106 74-75 0.06058736634880546 1106 76-77 0.029464006306813673 1106 78-79 0.09437294195051749 1106 80-81 -0.12284125433112436 1106 82-83 -0.4109506251802806 1106 84-85 0.006492468594487377 1106 86-87 0.017460373278456842 1106 88-89 -0.4208680384883188 1106 90-91 -0.26060302207101316 1106 92-93 -0.20820904481465874 1106 94-95 -0.006160922220185938 1106 96-97 0.2520691239856525 1106 98-99 0.43146304317908246 1106 100-101 0.7083966589920649 1107 1 0.35506669448830763 1107 2 0.1439847975365396 1107 3 0.23347879556860818 1107 4 0.059161277228255926 1107 5 0.08146664440922535 1107 6 -0.023746766171264255 1107 7 0.13421253106965025 1107 8 0.054670979620311755 1107 9 0.2403280324686392 1107 10-11 0.1770332873856404 1107 12-13 0.25924013451675165 1107 14-15 0.3121212438572698 1107 16-17 0.21354534577090334 1107 18-19 0.36817977834262905 1107 20-21 0.482474604522821 1107 22-23 0.25505472788169925 1107 24-25 0.39420239227088416 1107 26-27 0.38390002847395266 1107 28-29 0.2334961254771244 1107 30-31 0.32287142275595926 1107 32-33 0.41604537099535577 1107 34-35 0.3447507548893327 1107 36-37 0.42062193075201293 1107 38-39 0.3820514796850816 1107 40-41 0.32629767289505907 1107 42-43 0.36324727978869475 1107 44-45 0.42344572749887277 1107 46-47 0.27186939200401383 1107 48-49 0.16180679200293469 1107 50-51 0.14829465997909352 1107 52-53 0.1648313091275 1107 54-55 0.13042531773086097 1107 56-57 0.1588277187995928 1107 58-59 0.15215734414160664 1107 60-61 0.46391042358629875 1107 62-63 0.2851295933987643 1107 64-65 0.3513242482690373 1107 66-67 0.3160031061715216 1107 68-69 0.548093037804172 1107 70-71 0.5160737528169577 1107 72-73 0.6176326252584516 1107 74-75 0.5650804539064076 1107 76-77 0.4301168480886872 1107 78-79 0.7323593730821081 1107 80-81 0.5293092833032809 1107 82-83 0.3900862411791657 1107 84-85 0.362457650366121 1107 86-87 0.8709101940669868 1107 88-89 0.599837286652491 1107 90-91 0.41878125852294446 1107 92-93 0.724413843050165 1107 94-95 0.2785933020501652 1107 96-97 0.46237378348386216 1107 98-99 0.9649179842953153 1107 100-101 1.2800364439383038 1108 1 0.026832464699673153 1108 2 0.07554783726020275 1108 3 -0.022422183130295537 1108 4 -0.044959127410280075 1108 5 0.04793181424227555 1108 6 -0.021680395581576306 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-0.4059023191693747 2316 82-83 -0.2909561472455344 2316 84-85 -0.3644913793646509 2316 86-87 -0.5585853678287158 2316 88-89 -0.6363148656016584 2316 90-91 -0.18555240313952837 2316 92-93 -0.057942144994125755 2316 94-95 -0.04774322400105291 2316 96-97 -0.006975558725681452 2316 98-99 -0.07309035979362832 2316 100-101 0.3126981177376358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 5.0 5 17.0 6 46.0 7 92.0 8 190.0 9 338.0 10 585.0 11 1165.0 12 1844.0 13 2721.0 14 3418.0 15 4725.0 16 6774.0 17 10152.0 18 9360.0 19 5209.0 20 3862.0 21 3686.0 22 3977.0 23 4123.0 24 4640.0 25 5245.0 26 6346.0 27 7867.0 28 9527.0 29 11755.0 30 14674.0 31 18643.0 32 23635.0 33 31527.0 34 43657.0 35 64536.0 36 97077.0 37 149975.0 38 125388.0 39 11633.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95060114053249 19.883476807961934 15.534924267189714 20.630997784315863 2 15.07844048691206 21.322884285755787 41.54818279538654 22.050492431945614 3 19.181168472734665 26.79657767060806 31.187356555591062 22.83489730106621 4 11.993039092641657 17.745520150606612 38.42211685957328 31.839323897178453 5 12.150388130739694 38.518870432762505 34.70076231405247 14.629979122445333 6 29.151244879579323 37.70910200168502 18.251939223148657 14.887713895586996 7 26.368350716132593 34.681008686557625 21.532349437843177 17.418291159466605 8 25.92385462363092 35.30969466314169 20.25667470439557 18.509776008831818 9 26.402196333633537 17.062403765143372 21.456523633828187 35.078876267394904 10-11 24.07550919170651 27.873193597799016 27.54454224495384 20.506754965540633 12-13 25.712218793148406 25.488509858809156 28.806883262431644 19.992388085610795 14-15 22.309055518433514 26.21422968536649 28.647555271491242 22.829159524708754 16-17 19.99513378460823 29.661979605473405 29.209276313878156 21.13361029604021 18-19 19.704250312309345 27.276517242381104 33.345414136718865 19.673818308590686 20-21 20.612198948316436 26.149806803985935 33.78068620900032 19.457308038697306 22-23 20.2836088220298 26.169029347208728 33.74253536757731 19.804826463184163 24-25 20.084556001101074 26.249692229810456 34.10878105573709 19.55697071335138 26-27 20.105110710514122 26.33629035889584 34.01622853590193 19.542370394688103 28-29 19.598395441112064 26.57777629288211 34.32907692832868 19.494751337677137 30-31 19.88052351762006 26.40749832950815 34.10396269719067 19.608015455681123 32-33 19.754147177595073 26.590744022544378 33.826588419859974 19.82852038000058 34-35 20.151433717788557 26.43734929258302 33.78838499753058 19.622831992097847 36-37 20.126158449783563 26.337192411609188 33.85578571221057 19.680863426396677 38-39 19.95064052561013 26.723260107579833 33.8599748118515 19.466124554958537 40-41 19.952659335327773 26.685812108838498 33.806812610716364 19.55471594511737 42-43 19.871813498301595 26.731818089362974 33.429973238180196 19.96639517415523 44-45 19.97930042706487 26.938714738095932 32.941372999041285 20.140611835797912 46-47 20.161601929054935 27.233665494901366 32.494625374044915 20.11010720199878 48-49 20.25732837511984 27.541544406031203 31.97691816042532 20.224209058423636 50-51 20.345719183056854 27.733578338804797 31.714723569913716 20.20597890822463 52-53 20.33722146364138 28.161151622555998 31.251198396327823 20.2504285174748 54-55 20.172786961447954 27.901281194619564 31.47627901571715 20.449652828215335 56-57 20.554893814822346 28.129194387147383 31.053281427035824 20.26263037099445 58-59 20.57820807065454 27.88501205659336 31.194329043316582 20.342450829435517 60-61 20.432947909706282 27.879056389994478 31.700342813979837 19.9876528863194 62-63 20.46802823857529 27.979576421370673 31.523996978588652 20.028398361465385 64-65 20.33671305307806 27.839109845733713 31.551451149007875 20.272725952180355 66-67 20.204090526132305 28.084890038058163 31.32063275326109 20.390386682548446 68-69 20.259173555639805 28.04970519407512 31.287486254737303 20.40363499554777 70-71 20.367653467360043 28.198919264402544 31.13186717410883 20.301560094128586 72-73 20.32451119955841 28.01255047790593 31.048560471805004 20.61437785073066 74-75 20.25747363528079 28.50098776909445 30.63268063101014 20.608857964614625 76-77 20.24236657854217 28.07501234711368 31.001133029255396 20.681488045088752 78-79 20.10044740129572 28.161224252636472 30.933587054414456 20.80474129165335 80-81 20.152755696199772 28.361122832807613 30.81943495208953 20.66668651890308 82-83 20.236488694881647 28.337243337860706 30.881457862740685 20.544810104516962 84-85 20.148674854557207 28.316349876164814 30.576832288663088 20.95814298061489 86-87 20.136108770808516 28.185410069434358 30.639725748816133 21.038755410940997 88-89 20.15499259173179 27.91922082449667 30.849336161064468 21.07645042270707 90-91 20.014440737407394 27.917185131270518 31.02724635912545 21.041127772196642 92-93 20.220467270916753 27.85888758231933 30.89592338192174 21.024721764842173 94-95 19.782690799221406 28.00013073414485 30.836262746579123 21.38091572005462 96-97 19.808329217628774 27.886973068766157 30.939470090932865 21.365227622672204 98-99 19.852746780243056 27.922403349186382 30.588272037140875 21.63657783342969 100-101 19.575485747578345 27.920914642385462 31.127146508124003 21.376453101912187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 964.0 1 721.5 2 1226.5 3 1664.5 4 971.5 5 627.0 6 980.0 7 2001.0 8 3071.5 9 3337.5 10 2770.0 11 2142.0 12 2002.0 13 2093.0 14 2204.5 15 2253.0 16 2306.0 17 2348.0 18 2378.0 19 2425.0 20 2529.5 21 2839.5 22 3187.5 23 3618.5 24 4173.5 25 4792.0 26 5783.0 27 6980.0 28 7976.0 29 9143.0 30 10368.5 31 11385.0 32 12703.5 33 14340.5 34 16091.0 35 17717.5 36 19808.0 37 21607.0 38 22455.5 39 22666.0 40 22953.5 41 23709.5 42 24098.0 43 24697.0 44 25599.0 45 26036.5 46 25629.0 47 26097.0 48 28775.0 49 27710.0 50 23820.0 51 21363.0 52 18937.5 53 17256.5 54 15747.5 55 13903.5 56 12014.0 57 10324.5 58 8830.5 59 7584.0 60 6296.0 61 4986.0 62 3860.0 63 2779.0 64 1909.0 65 1377.5 66 1032.5 67 782.5 68 604.0 69 426.0 70 277.5 71 198.0 72 156.0 73 134.5 74 102.5 75 77.5 76 59.5 77 38.5 78 22.5 79 14.5 80 9.0 81 5.5 82 4.5 83 4.5 84 2.5 85 0.5 86 1.5 87 1.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02106272333749746 2 0.0 3 0.0 4 5.810406437930333E-4 5 0.01757647947473926 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.263008047412917E-5 12-13 0.0037041341041805873 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010168211266378083 24-25 7.263008047412917E-5 26-27 4.3578048284477505E-4 28-29 9.44191046163679E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4526016094825833E-4 40-41 7.989308852154208E-4 42-43 0.01830278027948055 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.4526016094825833E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.263008047412917E-5 82-83 0.0021789024142238747 84-85 7.263008047412917E-4 86-87 0.0 88-89 0.0 90-91 0.01300078440486912 92-93 0.0026873129775427792 94-95 0.0 96-97 0.0 98-99 0.0020336422532756166 100-101 0.02193428430318701 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.53480299951025 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37315998790132 54.45005258265372 2 9.52742389436414 12.29700848231233 3 2.556123841672189 4.948768456939983 4 1.0893141549686836 2.8119469758192794 5 0.6161594027962936 1.9881862837877347 6 0.3809628604153765 1.4751217888221748 7 0.23481497725456632 1.0607616808920544 8 0.177345036733916 0.9155941606851342 9 0.14317949276005137 0.8316054322955748 >10 0.8256651187578906 9.868361920722178 >50 0.04882647406780376 2.1828396083618538 >100 0.023618276168450282 2.606529925195598 >500 0.0013625928557480526 0.517348925242116 >1k 0.0015896916650393945 2.002565358217641 >5k 4.5419761858268414E-4 2.0433084180525722 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7539 1.0951163533889194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6403 0.930100810551698 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4773 0.693326748206037 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 0.3586473373812498 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1466 0.2129513959501467 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1307 0.18985503035937365 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1282 0.1862235263356672 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1280 0.18593300601377066 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1087 0.15789779495075681 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.9052032189651666E-4 0.0 0.0 0.0 0.0 12-13 2.9052032189651666E-4 0.0 0.0 0.0 0.0 14-15 2.9052032189651666E-4 0.0 0.0 7.263008047412917E-5 0.0 16-17 2.9052032189651666E-4 0.0 0.0 1.4526016094825833E-4 0.0 18-19 2.9052032189651666E-4 0.0 0.0 2.178902414223875E-4 0.0 20-21 2.9052032189651666E-4 0.0 0.0 4.35780482844775E-4 0.0 22-23 2.9052032189651666E-4 0.0 0.0 5.810406437930333E-4 0.0 24-25 2.9052032189651666E-4 0.0 0.0 8.7156096568955E-4 0.0 26-27 2.9052032189651666E-4 0.0 0.0 0.0015978617704308416 1.4526016094825833E-4 28-29 2.9052032189651666E-4 0.0 0.0 0.0026146828970686504 1.4526016094825833E-4 30-31 2.9052032189651666E-4 0.0 0.0 0.005156735713663171 1.4526016094825833E-4 32-33 2.9052032189651666E-4 0.0 0.0 0.008279829174050724 1.4526016094825833E-4 34-35 2.9052032189651666E-4 0.0 0.0 0.011620812875860666 1.4526016094825833E-4 36-37 2.9052032189651666E-4 0.0 0.0 0.01481653641672235 1.4526016094825833E-4 38-39 2.9052032189651666E-4 0.0 0.0 0.020409052613230297 1.4526016094825833E-4 40-41 2.9052032189651666E-4 0.0 0.0 0.025711048487841726 1.4526016094825833E-4 42-43 2.9052032189651666E-4 0.0 0.0 0.03558873943232329 1.4526016094825833E-4 44-45 4.35780482844775E-4 0.0 0.0 0.048299003515295896 1.4526016094825833E-4 46-47 4.35780482844775E-4 0.0 0.0 0.06021033671305308 1.4526016094825833E-4 48-49 5.810406437930333E-4 0.0 0.0 0.0766973649806804 1.4526016094825833E-4 50-51 5.810406437930333E-4 0.0 0.0 0.09754219807675547 1.4526016094825833E-4 52-53 5.810406437930333E-4 0.0 0.0 0.13204148630196683 1.4526016094825833E-4 54-55 5.810406437930333E-4 0.0 0.0 0.15165160802998168 1.4526016094825833E-4 56-57 5.810406437930333E-4 0.0 0.0 0.19530228639493333 1.4526016094825833E-4 58-59 5.810406437930333E-4 0.0 0.0 0.2542779117399262 1.4526016094825833E-4 60-61 5.810406437930333E-4 0.0 0.0 0.3155050695796171 1.4526016094825833E-4 62-63 5.810406437930333E-4 0.0 0.0 0.4072368612184422 1.4526016094825833E-4 64-65 5.810406437930333E-4 0.0 0.0 0.47296708404752913 2.9052032189651666E-4 66-67 5.810406437930333E-4 0.0 0.0 0.5835826966096278 2.9052032189651666E-4 68-69 5.810406437930333E-4 0.0 0.0 0.7194735771767236 2.9052032189651666E-4 70-71 5.810406437930333E-4 0.0 0.0 0.8266755759565382 2.9052032189651666E-4 72-73 5.810406437930333E-4 0.0 0.0 0.9884953952528979 2.9052032189651666E-4 74-75 5.810406437930333E-4 0.0 0.0 1.159030824206153 2.9052032189651666E-4 76-77 5.810406437930333E-4 0.0 0.0 1.2862060951163534 2.9052032189651666E-4 78-79 5.810406437930333E-4 0.0 0.0 1.4244937683390952 2.9052032189651666E-4 80-81 5.810406437930333E-4 0.0 0.0 1.648557566601784 2.9052032189651666E-4 82-83 5.810406437930333E-4 0.0 0.0 1.8369599953516746 2.9052032189651666E-4 84-85 5.810406437930333E-4 0.0 0.0 2.087388512826472 2.9052032189651666E-4 86-87 5.810406437930333E-4 0.0 0.0 2.413424944074838 2.9052032189651666E-4 88-89 5.810406437930333E-4 0.0 0.0 2.8399814066993985 2.9052032189651666E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAGG 40 5.3768425E-4 47.517586 1 TGGATCG 40 5.3806964E-4 47.510677 5 TATCACG 75 1.3593308E-7 44.343304 2 GGATCGC 45 9.625496E-4 42.219444 6 GTACCTG 760 0.0 40.01481 1 TACCTGG 775 0.0 39.234627 2 CTAGCGC 55 0.0025760676 34.553223 3 GGACCGA 85 1.6319897E-5 33.527206 6 ACCTGGG 950 0.0 31.507082 3 GGTATCA 4765 0.0 30.814133 1 TACACCG 65 0.0058360505 29.23734 5 ATCACGC 115 3.8548515E-6 28.919546 3 GTATCAA 6825 0.0 28.614985 1 ACTATAC 150 1.0570038E-6 25.339031 3 GAGTACT 5105 0.0 23.775152 12-13 GTACATG 3905 0.0 23.606686 1 TCAACGC 8215 0.0 23.538462 4 TATCAAC 8255 0.0 23.48196 2 ATCAACG 8235 0.0 23.481295 3 CAACGCA 8325 0.0 23.284512 5 >>END_MODULE