FastQCFastQC Report
Thu 26 May 2016
SRR937641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662529
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54190.8179264605775748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42190.6368023135591045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.4605081437944603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.21040588411978944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11940.18021852628337778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11930.1800675894941957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.15063491560369432No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7950.11999474739973646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.11803256914036969No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.11425914941081824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG5450.041.8570252
GTACCTG6350.037.4242171
ACCTGGG7600.030.6365853
TAACGCA650.005835824629.2374024
GTATCAA57800.028.615931
GTACATG42100.027.2076721
TACATGG43450.026.0321942
AACGCGG559.4849995E-525.90615334-35
ATAACGC950.001223302925.0094593
ACATGGG43750.024.8722653
CATGGGG13400.024.8190654
TATCAAC67100.024.3646142
ATCAACG67750.024.1272053
TCAACGC68100.023.9298044
CAACGCA68250.023.8790195
TAACGCG500.001653776523.7576-77
AACGCAG70000.023.276766
GGTATCA40300.022.8798481
GAGTACT34850.022.55568312-13
AGTACTT37150.022.05418612-13
Produced by FastQC (version 0.11.5)