##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937641_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662529 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820656907093877 33.0 31.0 34.0 30.0 34.0 2 31.925680234374646 33.0 31.0 34.0 30.0 34.0 3 31.930736616812244 34.0 31.0 34.0 30.0 34.0 4 35.52664713544615 37.0 35.0 37.0 33.0 37.0 5 35.53765797421698 37.0 35.0 37.0 33.0 37.0 6 35.60184082508086 37.0 35.0 37.0 33.0 37.0 7 35.562843286859895 37.0 35.0 37.0 33.0 37.0 8 35.55063702871874 37.0 35.0 37.0 33.0 37.0 9 37.17764203529204 39.0 37.0 39.0 34.0 39.0 10-11 37.068347951561364 39.0 37.0 39.0 33.0 39.0 12-13 36.945064291525355 39.0 37.0 39.0 33.0 39.0 14-15 38.080301390580644 40.0 38.0 41.0 33.0 41.0 16-17 38.031259763723554 40.0 38.0 41.0 33.0 41.0 18-19 38.014569173575794 40.0 38.0 41.0 33.0 41.0 20-21 37.9618748764205 40.0 38.0 41.0 33.0 41.0 22-23 37.76313414205265 40.0 37.5 41.0 33.0 41.0 24-25 37.59639728977901 40.0 37.0 41.0 32.0 41.0 26-27 37.53717874991133 40.0 37.0 41.0 32.0 41.0 28-29 37.578026773167664 40.0 37.0 41.0 32.5 41.0 30-31 37.378135145782295 40.0 37.0 41.0 32.0 41.0 32-33 37.115785875033396 39.0 36.0 41.0 31.5 41.0 34-35 37.09216502221035 39.5 36.0 41.0 31.5 41.0 36-37 36.989510647835786 40.0 36.0 41.0 31.0 41.0 38-39 36.857534538110784 40.0 36.0 41.0 31.0 41.0 40-41 36.62195164287148 39.0 35.5 41.0 30.5 41.0 42-43 36.377609131072 39.0 35.0 41.0 30.0 41.0 44-45 36.11225848227021 39.0 35.0 41.0 30.0 41.0 46-47 35.714929459691575 39.0 35.0 41.0 28.0 41.0 48-49 35.32808526117347 39.0 35.0 40.5 26.0 41.0 50-51 34.396910172988655 38.0 34.0 39.5 24.5 40.5 52-53 34.494961729977106 38.0 34.5 39.5 24.0 40.5 54-55 34.85958878781155 39.0 35.0 40.0 24.5 41.0 56-57 34.6772631839512 38.0 34.5 40.0 24.0 41.0 58-59 34.46299105397651 38.0 34.0 40.0 23.0 41.0 60-61 34.15715010210874 38.0 34.0 40.0 22.5 41.0 62-63 33.809518526736184 38.0 34.0 40.0 21.5 41.0 64-65 33.4565060548293 37.0 33.0 40.0 20.0 41.0 66-67 33.048314866217176 36.5 33.0 39.5 18.5 41.0 68-69 32.58115191938768 36.0 32.0 39.0 15.0 40.5 70-71 32.150421340047004 35.5 32.0 39.0 12.0 40.0 72-73 31.699389007877393 35.0 31.0 38.0 8.0 40.0 74-75 31.239937421607205 35.0 31.0 37.5 7.0 39.5 76-77 30.7457877315559 35.0 30.5 37.0 2.0 39.0 78-79 30.217696885721228 34.5 30.0 36.0 2.0 39.0 80-81 29.670766864544795 34.0 29.0 36.0 2.0 37.5 82-83 29.14941232761132 34.0 29.0 35.0 2.0 37.0 84-85 28.679811751636535 34.0 29.0 35.0 2.0 37.0 86-87 28.208230884987678 34.0 27.5 35.0 2.0 36.0 88-89 27.726175005169587 34.0 26.5 35.0 2.0 36.0 90-91 27.230798953706177 33.0 26.0 35.0 2.0 35.5 92-93 26.669447677007348 33.0 25.0 35.0 2.0 35.0 94-95 26.058497062015398 32.5 24.5 35.0 2.0 35.0 96-97 25.347086693563604 32.0 21.0 34.5 2.0 35.0 98-99 24.440635051446804 32.0 9.5 34.0 2.0 35.0 100-101 22.095410917861706 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15209155751095338 1101 2 0.00916439090841692 1101 3 0.23775747960975835 1101 4 0.20271665577142528 1101 5 -0.0071533570283008885 1101 6 0.06156066885626643 1101 7 0.10169834228611307 1101 8 0.11610273688770434 1101 9 0.24386115527667585 1101 10-11 0.08865067060348508 1101 12-13 0.12128141213307941 1101 14-15 0.32674463707657964 1101 16-17 0.5015662217179369 1101 18-19 0.2425069669818143 1101 20-21 0.5620463086332492 1101 22-23 0.526115208841631 1101 24-25 0.4308812747953965 1101 26-27 0.4551285661173523 1101 28-29 0.4443318516884176 1101 30-31 0.35543260204118354 1101 32-33 0.21698418175199663 1101 34-35 0.44587522842088134 1101 36-37 0.2608406395747096 1101 38-39 0.3925330657287063 1101 40-41 0.4837513783275398 1101 42-43 0.5519918242105746 1101 44-45 0.4756571546981263 1101 46-47 0.6243242106678011 1101 48-49 0.9055204979470446 1101 50-51 0.8283486277669212 1101 52-53 0.8987090659081431 1101 54-55 0.6601766076997748 1101 56-57 0.623977512367226 1101 58-59 0.6661890353887472 1101 60-61 0.7856640522610121 1101 62-63 0.8628314387222815 1101 64-65 0.5551565165710386 1101 66-67 0.7624492948978059 1101 68-69 0.8417038849071403 1101 70-71 0.8817477813722405 1101 72-73 0.715719682168384 1101 74-75 0.8132548636384413 1101 76-77 0.9984702092360997 1101 78-79 0.8637962992356343 1101 80-81 0.8546308318285689 1101 82-83 0.9308779437119057 1101 84-85 1.2053174845995756 1101 86-87 1.2052227556752406 1101 88-89 1.1431942227150955 1101 90-91 1.4568481124858224 1101 92-93 1.3578248454903807 1101 94-95 1.6344450617127642 1101 96-97 1.8717547632906033 1101 98-99 1.9438060417955327 1101 100-101 2.4743975986025326 1102 1 0.28222313645832386 1102 2 0.17166439090841834 1102 3 0.24598116382027868 1102 4 0.12429560313984922 1102 5 0.35659664297170224 1102 6 0.20175803727732244 1102 7 0.04222465807558251 1102 8 0.26570800004559914 1102 9 0.40011115527667585 1102 10-11 0.4462164600771672 1102 12-13 0.5444064121330854 1102 14-15 0.5830604265502615 1102 16-17 0.45712543224425417 1102 18-19 0.42737538803444863 1102 20-21 0.5879344665279831 1102 22-23 0.486411261473215 1102 24-25 0.590026011637498 1102 26-27 0.5720693555910401 1102 28-29 0.3401542201094685 1102 30-31 0.4613536546727559 1102 32-33 0.6523131291204223 1102 34-35 0.7075528599998293 1102 36-37 0.7616959027326118 1102 38-39 0.5231909604655485 1102 40-41 0.7296066414854323 1102 42-43 0.5486036663158416 1102 44-45 0.5608216283823282 1102 46-47 0.6385676317204343 1102 48-49 0.6540073400523028 1102 50-51 0.72864468039851 1102 52-53 0.6726893290660314 1102 54-55 0.5881042392787208 1102 56-57 0.674668301840903 1102 58-59 0.6620442985466397 1102 60-61 0.8109272101557536 1102 62-63 0.8501998597749107 1102 64-65 0.9408473060447236 1102 66-67 0.9701466633188573 1102 68-69 1.089697305959774 1102 70-71 1.0508596234774998 1102 72-73 0.88114731374732 1102 74-75 1.10147854784897 1102 76-77 0.9688320513413622 1102 78-79 1.215375246604058 1102 80-81 1.041867673933833 1102 82-83 1.0225226805540117 1102 84-85 1.2402845898627355 1102 86-87 1.3040056504120834 1102 88-89 1.3491152753466729 1102 90-91 1.289973112485825 1102 92-93 1.47427221391143 1102 94-95 1.4279647985548714 1102 96-97 1.656327131711656 1102 98-99 1.9256810417955315 1102 100-101 2.6210423354446384 1103 1 0.43009425656628864 1103 2 0.5248189118260953 1103 3 0.31005842427912356 1103 4 0.38173150057574645 1103 5 0.224931663214619 1103 6 0.3493811816767831 1103 7 0.36839874714441123 1103 8 0.34484767615896317 1103 9 0.3080986721457748 1103 10-11 0.44706834941590046 1103 12-13 0.6104514526189107 1103 14-15 0.8130638003694273 1103 16-17 0.6618732392618014 1103 18-19 0.4561709345931533 1103 20-21 0.4516043383228521 1103 22-23 0.49792694973232443 1103 24-25 0.7319440278318368 1103 26-27 0.7915152057934733 1103 28-29 0.4732792201094682 1103 30-31 0.6394406992071779 1103 32-33 0.6364679874200121 1103 34-35 0.6845223269364027 1103 36-37 0.8019674951752549 1103 38-39 0.9091153869162909 1103 40-41 1.03921696537207 1103 42-43 0.9422752250202961 1103 44-45 0.8257077619855693 1103 46-47 0.9826406749053191 1103 48-49 1.062639256381587 1103 50-51 0.8910679260125391 1103 52-53 1.0283480672576673 1103 54-55 0.9312461083745376 1103 56-57 1.012270359870591 1103 58-59 0.979734919329367 1103 60-61 1.0690648619776155 1103 62-63 0.8792788071433364 1103 64-65 0.8550721710919547 1103 66-67 0.8871625202689231 1103 68-69 1.0855635340299514 1103 70-71 1.2682018974316165 1103 72-73 1.3526798711022536 1103 74-75 1.398258237457597 1103 76-77 1.5135343118002034 1103 78-79 1.4772747067929934 1103 80-81 1.3240808993049562 1103 82-83 1.5473421812287782 1103 84-85 1.5075104670557167 1103 86-87 1.62234488792895 1103 88-89 1.926611901527508 1103 90-91 2.052462147573543 1103 92-93 2.1492553448156144 1103 94-95 1.9322520792566245 1103 96-97 2.277304695814216 1103 98-99 2.396775002659229 1103 100-101 2.8179704730964588 1104 1 0.13766774287231698 1104 2 0.0858407757480677 1104 3 -0.02750644550916448 1104 4 0.07321688593868458 1104 5 -0.0044565640253821925 1104 6 5.61422335891848E-5 1104 7 -0.055486712186805676 1104 8 -0.043249725901922886 1104 9 -0.0220280575513101 1104 10-11 -0.14593004138055932 1104 12-13 -0.005030541219689155 1104 14-15 -0.02350662884332877 1104 16-17 0.03898949929964601 1104 18-19 -0.04270478689266355 1104 20-21 0.04482404378746452 1104 22-23 0.13094296701256525 1104 24-25 0.12744219239552024 1104 26-27 -0.01334427414656858 1104 28-29 0.0651159548033462 1104 30-31 0.03245423776314027 1104 32-33 -0.14556952394080014 1104 34-35 -0.005484676443316516 1104 36-37 0.08814998436525912 1104 38-39 -0.024010205715207178 1104 40-41 0.04684789512974419 1104 42-43 0.08841962841496098 1104 44-45 0.1104335088488071 1104 46-47 0.011306334344347135 1104 48-49 -0.13552983195936008 1104 50-51 -0.12852186479100425 1104 52-53 0.04092365851209934 1104 54-55 0.05524527426414494 1104 56-57 -0.027039059675125543 1104 58-59 -0.009161969674927661 1104 60-61 0.17809922181756122 1104 62-63 0.49697099680115286 1104 64-65 0.2548323643537529 1104 66-67 0.2466080335812464 1104 68-69 0.2849906733067158 1104 70-71 0.14481552726758906 1104 72-73 0.542639660686099 1104 74-75 0.381044874379576 1104 76-77 0.637339704402585 1104 78-79 0.5559073165749027 1104 80-81 0.34729041445861597 1104 82-83 0.5811925056269018 1104 84-85 0.6710954499210438 1104 86-87 0.8350351693625164 1104 88-89 0.744231893422473 1104 90-91 0.8578685206490917 1104 92-93 0.9487255666811087 1104 94-95 1.040226095930965 1104 96-97 1.3400242891460579 1104 98-99 1.4771879659937817 1104 100-101 1.8641072042493008 1105 1 0.09290205019634357 1105 2 -0.1799729892832751 1105 3 -0.046331136499208725 1105 4 -0.09877148951191117 1105 5 0.0013929688502969384 1105 6 0.15033231842748052 1105 7 0.004317309832771343 1105 8 0.02050831953442156 1105 9 0.1122317623054343 1105 10-11 0.148952083080367 1105 12-13 0.15538684344298304 1105 14-15 0.07179045849914445 1105 16-17 0.11738302010368074 1105 18-19 0.2504983912293355 1105 20-21 0.14290850806153088 1105 22-23 0.23387332217608758 1105 24-25 0.43266578799533306 1105 26-27 0.24873668785941305 1105 28-29 0.1897328942308789 1105 30-31 0.10037122655774766 1105 32-33 0.09820370420029434 1105 34-35 -0.07192996747620128 1105 36-37 -0.11694227618112762 1105 38-39 0.07418137580101103 1105 40-41 0.28368411752376943 1105 42-43 0.3480066215874018 1105 44-45 0.24364311400533722 1105 46-47 0.04345780743928884 1105 48-49 0.1581646882951091 1105 50-51 0.1869453992483443 1105 52-53 0.3033143290660334 1105 54-55 0.1426110284161055 1105 56-57 0.26906326989202256 1105 58-59 0.21001155094280932 1105 60-61 0.12974510152955077 1105 62-63 0.44738037095702055 1105 64-65 0.38846112393609644 1105 66-67 0.3018199860025561 1105 68-69 0.37325760947414466 1105 70-71 0.26278654041040994 1105 72-73 0.39503908691026624 1105 74-75 0.6269697619064729 1105 76-77 0.7314259011816162 1105 78-79 1.0940573552302553 1105 80-81 1.00004658767185 1105 82-83 0.9782295495635971 1105 84-85 0.7265845099905306 1105 86-87 0.6477336855558526 1105 88-89 0.6535082465926791 1105 90-91 1.0256979527414138 1105 92-93 1.179104482282039 1105 94-95 1.2508861244334675 1105 96-97 0.9592085214880122 1105 98-99 1.250355562562305 1105 100-101 1.772507990396715 1106 1 0.3147395568195712 1106 2 0.21097063064569 1106 3 0.01848732145575127 1106 4 0.20843353417433264 1106 5 0.09017833426890576 1106 6 0.18726501593084777 1106 7 0.24510643147460343 1106 8 0.16920964208172506 1106 9 0.13778562161493824 1106 10-11 -0.008945690990152855 1106 12-13 0.16935201968644265 1106 14-15 -0.0648172417584405 1106 16-17 0.012750432244253318 1106 18-19 0.1437752238308363 1106 20-21 0.3564925535558956 1106 22-23 0.24143691828765412 1106 24-25 0.030545305561972214 1106 26-27 0.1368014984481789 1106 28-29 0.16868151896004946 1106 30-31 0.6007830471193927 1106 32-33 0.6733373491532646 1106 34-35 0.5578761358618962 1106 36-37 0.4014270193171754 1106 38-39 0.5478707634212157 1106 40-41 0.49892724903880037 1106 42-43 0.5252179930810286 1106 44-45 0.46342938700302483 1106 46-47 0.6215489535595182 1106 48-49 0.8147483088536163 1106 50-51 0.6110143437811004 1106 52-53 0.8308184341563489 1106 54-55 0.7815781719552461 1106 56-57 0.727510050609375 1106 58-59 0.7665660555252956 1106 60-61 0.8408451083495123 1106 62-63 1.06132875961071 1106 64-65 0.8505815014470173 1106 66-67 0.9497094712006344 1106 68-69 0.9307800235295574 1106 70-71 0.9571291226564895 1106 72-73 1.1575235452744153 1106 74-75 1.4756965281445318 1106 76-77 1.3984820923922605 1106 78-79 1.5611182679505262 1106 80-81 1.5580909908468037 1106 82-83 1.3436166871221573 1106 84-85 1.5995077015211905 1106 86-87 1.714747645485975 1106 88-89 1.7552318599115324 1106 90-91 1.8322996724201417 1106 92-93 1.798923281234913 1106 94-95 1.9106895522494511 1106 96-97 1.8880789790022945 1106 98-99 2.34996367726351 1106 100-101 2.615933550551368 1107 1 0.24379951301249392 1107 2 0.3152034672849737 1107 3 0.3988563858451464 1107 4 0.25694213955192424 1107 5 0.27424095913511337 1107 6 0.1739449822151542 1107 7 0.29335254439885716 1107 8 0.42446022029427155 1107 9 0.19199614639568097 1107 10-11 0.3334611314803695 1107 12-13 0.4719474867334412 1107 14-15 0.5046368031044395 1107 16-17 0.5837983896154739 1107 18-19 0.5477350683186373 1107 20-21 0.6206620420164413 1107 22-23 0.4462014479740972 1107 24-25 0.6349793864154805 1107 26-27 0.5374612294809111 1107 28-29 0.5860678524362868 1107 30-31 0.6001413989001136 1107 32-33 0.7042691681967952 1107 34-35 0.7377315900175958 1107 36-37 0.5433100235141382 1107 38-39 0.6097096105543542 1107 40-41 0.5013450961923596 1107 42-43 0.4753254691577524 1107 44-45 0.6918240706558691 1107 46-47 0.8048853493048043 1107 48-49 0.779431407547861 1107 50-51 0.7401533393683124 1107 52-53 0.6119288938972929 1107 54-55 0.7354699142343151 1107 56-57 0.8433217210238482 1107 58-59 0.7174639255448696 1107 60-61 1.0916376897294668 1107 62-63 1.0212344956541273 1107 64-65 1.0378888246948108 1107 66-67 0.8369339634964774 1107 68-69 0.9260172437928063 1107 70-71 1.0088780515414513 1107 72-73 1.0137039300883544 1107 74-75 0.9290384945630024 1107 76-77 1.0703606925491762 1107 78-79 1.1357171648989066 1107 80-81 1.0217255780190904 1107 82-83 0.7031598919216862 1107 84-85 0.9373194477668214 1107 86-87 1.0199126219928978 1107 88-89 1.1373479574070657 1107 90-91 1.2722122332673536 1107 92-93 1.270431183716049 1107 94-95 1.7728460152511438 1107 96-97 1.8455289523155614 1107 98-99 2.1710706954367396 1107 100-101 3.08879766404144 1108 1 0.11953795127313782 1108 2 0.023979205723232866 1108 3 0.10593486752398817 1108 4 0.17874004758429152 1108 5 0.23266145778651293 1108 6 0.263656185425468 1108 7 0.02055799140892134 1108 8 -0.008458666621066868 1108 9 -0.006138844723324155 1108 10-11 0.1293183119290191 1108 12-13 0.1805406713923361 1108 14-15 0.058153019142856976 1108 16-17 0.3488615433553619 1108 18-19 0.044505017664079105 1108 20-21 -0.0016720149534990014 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74-75 -0.31453335691293915 2316 76-77 -0.45969911112362283 2316 78-79 -0.2749678906508457 2316 80-81 0.0418396627293518 2316 82-83 -0.227473818045425 2316 84-85 -0.10272836531934004 2316 86-87 -0.39650030196887087 2316 88-89 -0.586528982356409 2316 90-91 -0.8030958651052131 2316 92-93 -0.49488044715299395 2316 94-95 -0.6629123022854664 2316 96-97 -0.32181187389058863 2316 98-99 -0.3928886360756181 2316 100-101 -0.540711866236034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2765.0 3 490.0 4 462.0 5 513.0 6 580.0 7 719.0 8 829.0 9 1061.0 10 1301.0 11 1813.0 12 2482.0 13 3321.0 14 3582.0 15 4630.0 16 5951.0 17 8310.0 18 7072.0 19 4582.0 20 4388.0 21 4591.0 22 4924.0 23 5386.0 24 5876.0 25 6816.0 26 7864.0 27 9115.0 28 10693.0 29 12813.0 30 15653.0 31 19465.0 32 24787.0 33 32560.0 34 44589.0 35 63881.0 36 90793.0 37 127275.0 38 106780.0 39 13811.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7039329328269 19.516517107274186 14.576971384471044 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31.53511723387042 19.790786120961464 20-21 21.385403263241134 27.246974826382324 31.503310567355193 19.86431134302135 22-23 20.905700771306172 27.42245549473436 31.457997718512182 20.213846015447288 24-25 20.67369913880691 27.460206105762254 31.782046785040396 20.08404797039044 26-27 20.591363989986927 27.544264597923444 31.787572268538433 20.076799143551195 28-29 20.114266789361817 27.966101054124742 31.889825401374196 20.029806755139244 30-31 20.35949105997919 27.70370605598703 31.815026856354795 20.12177602767898 32-33 20.187574759974172 27.834678949859594 31.677058082545596 20.30068820762064 34-35 20.588835711207384 27.779315142029777 31.568867617816288 20.06298152894655 36-37 20.61615387290456 27.68587356379191 31.607540026338988 20.090432536964546 38-39 20.434661322354412 27.97705130947998 31.663538147397485 19.924749220768128 40-41 20.50891662445854 28.04174446821539 31.479728759232238 19.96961014809383 42-43 20.29591771158986 28.098271293184595 31.169872945908452 20.435938049317098 44-45 20.38563515110369 28.29074749319763 30.830777326092594 20.492840029606086 46-47 20.627105996699203 28.49050170445396 30.431817683530266 20.450574615316576 48-49 20.619321356500674 28.625164353183745 30.237810267433535 20.517704022882047 50-51 20.643067561109866 28.81585075945003 30.13188298844039 20.409198690999716 52-53 20.663003145022675 29.1479098341131 29.826218403260118 20.36286861760411 54-55 20.49903528614804 28.97513518090653 29.848669965040465 20.67715956790497 56-57 20.682458780587172 29.304124549029805 29.598384254951508 20.415032415431515 58-59 20.89265791248031 28.911793963179207 29.738659418208425 20.456888706132062 60-61 20.721488242422225 28.95908754513932 30.157637039450456 20.161787172987996 62-63 20.69471775909788 29.02832199208771 30.12051380718849 20.15644644162591 64-65 20.539445904605973 29.076212872653002 30.027447005207446 20.35689421753358 66-67 20.438436813028975 29.22191412994506 29.711090414836004 20.62855864218996 68-69 20.313163827030394 29.20271964028418 29.903040620733552 20.581075911951874 70-71 20.466241733919126 29.341903549539033 29.631046296100315 20.560808420441525 72-73 20.493672053471943 29.16396419278806 29.575949721134933 20.766414032605063 74-75 20.340330957114674 29.595942033367855 29.42275273466734 20.640974274850134 76-77 20.37845893059797 29.38432377382285 29.448816635377927 20.78840066020125 78-79 20.44968236277936 29.337475748122227 29.35441236736132 20.858429521737094 80-81 20.425352721752397 29.66597465514087 29.290080848921235 20.6185917741855 82-83 20.506611426540967 29.64194721443098 29.239591444836737 20.611849914191318 84-85 20.474774307576705 29.373579736592635 29.207646100975342 20.943999854855317 86-87 20.387077557636015 29.433452475990034 29.28512894017466 20.89434102619929 88-89 20.61847018522201 29.129579801055883 29.25319218588279 20.998757827839324 90-91 20.482348538400768 29.083802388305696 29.450931620668406 20.982917452625134 92-93 20.690147872629954 29.209758383985 29.314236898605945 20.7858568447791 94-95 20.375453687064976 29.25324299785375 29.206825358546023 21.16447795653525 96-97 20.25987416762481 29.144111557362734 29.322057857493284 21.27395641751917 98-99 20.35972821945644 29.253450173567014 29.03883486338401 21.347986743592536 100-101 20.36442801582892 29.063531119249824 29.484577385204418 21.087463479716835 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1311.0 1 845.5 2 777.5 3 1052.0 4 705.0 5 545.0 6 878.5 7 1660.5 8 2531.5 9 2789.0 10 2268.0 11 1763.0 12 1698.0 13 1747.5 14 1786.5 15 1763.5 16 1759.5 17 1841.0 18 1891.0 19 2000.0 20 2151.5 21 2364.0 22 2748.5 23 3292.5 24 3845.0 25 4477.0 26 5552.0 27 6664.0 28 7550.5 29 8663.0 30 9983.0 31 11149.5 32 12345.5 33 13927.0 34 15877.0 35 17455.0 36 19242.5 37 20820.5 38 21351.5 39 21761.0 40 22242.5 41 22793.0 42 23144.5 43 23829.0 44 24751.5 45 25286.0 46 25099.5 47 25415.5 48 27626.0 49 26753.0 50 23237.0 51 20660.0 52 18311.0 53 16820.0 54 15400.5 55 13550.0 56 11829.5 57 10324.5 58 8984.5 59 7726.0 60 6416.5 61 5225.5 62 4035.0 63 2907.5 64 2111.0 65 1579.5 66 1148.5 67 858.5 68 650.0 69 442.5 70 295.5 71 222.5 72 171.5 73 131.5 74 98.5 75 74.5 76 54.0 77 40.5 78 28.0 79 18.5 80 10.0 81 10.5 82 10.0 83 6.5 84 3.5 85 2.5 86 2.0 87 0.5 88 1.0 89 2.0 90 1.5 91 1.5 92 3.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2564416048203173 2 0.2455741559992091 3 0.23410296002137265 4 0.22051864899498735 5 0.219462091470713 6 0.1903312911585757 7 0.18187883096438043 8 0.1918406590503963 9 0.1921425326287604 10-11 0.19410471088812717 12-13 0.19078410152612188 14-15 0.18844458129379996 16-17 0.18836911289920893 18-19 0.1877653657424807 20-21 0.17538854902955192 22-23 0.1688227987001324 24-25 0.16920014067308753 26-27 0.18014305788878673 28-29 0.19161425386662317 30-31 0.18610506106147806 32-33 0.18806723932084485 34-35 0.18474662995883953 36-37 0.17387918113773135 38-39 0.17214340806213765 40-41 0.1696529510406337 42-43 0.18059586825633292 44-45 0.17787900605105586 46-47 0.1771243221051456 48-49 0.18482209835343058 50-51 0.1903312911585757 52-53 0.20006671406081847 54-55 0.1800675894941957 56-57 0.1567478555655677 58-59 0.15923831258707166 60-61 0.16670968365158356 62-63 0.17395464953232237 64-65 0.1618797063977577 66-67 0.16769077278126693 68-69 0.16874733030554134 70-71 0.16467203699762578 72-73 0.16572859452190017 74-75 0.1681435831488131 76-77 0.18346366725079205 78-79 0.18716161858575248 80-81 0.1768979169213725 82-83 0.1771997904997366 84-85 0.16904920388390546 86-87 0.17161512930000045 88-89 0.1792374371536944 90-91 0.17833181641860205 92-93 0.17342637077018516 94-95 0.17244528164050177 96-97 0.16504937897058092 98-99 0.16799264635963104 100-101 0.1632381375003962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 662529.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.02142957565457 #Duplication Level Percentage of deduplicated Percentage of total 1 85.65462473328634 58.26350024124357 2 8.977009689559361 12.212580647966616 3 2.2418483237549953 4.57481183620799 4 0.9496378075107945 2.5838288498389814 5 0.5068687634458404 1.7238968948415176 6 0.3198458538089161 1.3053823331956977 7 0.2195171824601569 1.0452310797151774 8 0.16752014228861148 0.9115967648950735 9 0.13846251096601994 0.8476576144689082 >10 0.7642961709838483 9.40628996913444 >50 0.04165677693624391 1.99000096741724 >100 0.01581613327313964 1.870287844094209 >500 0.0015593370830931656 0.7692984998649732 >1k 0.001113812202209404 1.674515998031873 >5k 2.2276244044188082E-4 0.8211204590837679 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5419 0.8179264605775748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4219 0.6368023135591045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3051 0.4605081437944603 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1394 0.21040588411978944 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1194 0.18021852628337778 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1193 0.1800675894941957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.15063491560369432 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 795 0.11999474739973646 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 782 0.11803256914036969 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 757 0.11425914941081824 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5093678918205844E-4 0.0 0.0 0.0 0.0 7 3.018735783641169E-4 0.0 0.0 0.0 0.0 8 3.018735783641169E-4 0.0 0.0 0.0 0.0 9 3.018735783641169E-4 0.0 0.0 1.5093678918205844E-4 0.0 10-11 3.018735783641169E-4 0.0 0.0 1.5093678918205844E-4 0.0 12-13 3.018735783641169E-4 0.0 0.0 1.5093678918205844E-4 0.0 14-15 3.018735783641169E-4 0.0 0.0 4.5281036754617535E-4 0.0 16-17 3.018735783641169E-4 0.0 0.0 4.5281036754617535E-4 0.0 18-19 3.018735783641169E-4 0.0 0.0 4.5281036754617535E-4 0.0 20-21 3.018735783641169E-4 0.0 0.0 4.5281036754617535E-4 0.0 22-23 3.018735783641169E-4 0.0 0.0 0.0010565575242744091 0.0 24-25 3.018735783641169E-4 0.0 0.0 0.001660304681002643 0.0 26-27 3.018735783641169E-4 0.0 0.0 0.0021131150485488182 0.0 28-29 3.018735783641169E-4 0.0 0.0 0.003622482940369403 0.0 30-31 3.018735783641169E-4 0.0 0.0 0.006490281934828513 0.0 32-33 3.018735783641169E-4 0.0 0.0 0.009961828086015857 0.0 34-35 3.018735783641169E-4 0.0 0.0 0.012376816712928793 0.0 36-37 3.018735783641169E-4 0.0 0.0 0.016150236442480252 0.0 38-39 3.018735783641169E-4 0.0 0.0 0.021810366036807444 0.0 40-41 3.018735783641169E-4 0.0 0.0 0.027847837604089785 0.0 42-43 6.037471567282338E-4 0.0 0.0 0.03969637555488137 0.0 44-45 6.037471567282338E-4 0.0 0.0 0.05358256015963075 0.0 46-47 6.037471567282338E-4 0.0 0.0 0.06573297168878645 0.0 48-49 6.037471567282338E-4 0.0 0.0 0.0818832081312667 0.0 50-51 6.037471567282338E-4 0.0 0.0 0.10052390159525093 0.0 52-53 6.037471567282338E-4 0.0 0.0 0.12799439722638556 0.0 54-55 6.037471567282338E-4 0.0 0.0 0.1476916482146442 0.0 56-57 6.037471567282338E-4 0.0 0.0 0.19606688914749393 0.0 58-59 6.037471567282338E-4 0.0 0.0 0.2590829986310033 0.0 60-61 6.037471567282338E-4 0.0 0.0 0.3213444241686024 0.0 62-63 6.037471567282338E-4 0.0 0.0 0.4176420956667557 0.0 64-65 6.037471567282338E-4 0.0 0.0 0.48262038340963187 0.0 66-67 6.037471567282338E-4 0.0 0.0 0.5977851535555425 0.0 68-69 6.037471567282338E-4 0.0 0.0 0.7343829477653054 0.0 70-71 6.037471567282338E-4 0.0 0.0 0.8386048006955167 0.0 72-73 6.79215551319263E-4 0.0 0.0 0.9957299982340395 0.0 74-75 7.546839459102922E-4 0.0 0.0 1.1674960643232222 0.0 76-77 7.546839459102922E-4 0.0 0.0 1.2929245361335127 0.0 78-79 7.546839459102922E-4 0.0 0.0 1.4221264276733545 0.0 80-81 7.546839459102922E-4 0.0 0.0 1.6358529211551494 0.0 82-83 9.056207350923507E-4 0.0 0.0 1.8273917066271816 0.0 84-85 9.056207350923507E-4 0.0 0.0 2.0815692596097684 0.0 86-87 9.056207350923507E-4 0.0 0.0 2.420271414534307 0.0 88-89 9.056207350923507E-4 0.0 0.0 2.8573088876109574 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 545 0.0 41.857025 2 GTACCTG 635 0.0 37.424217 1 ACCTGGG 760 0.0 30.636585 3 TAACGCA 65 0.0058358246 29.237402 4 GTATCAA 5780 0.0 28.61593 1 GTACATG 4210 0.0 27.207672 1 TACATGG 4345 0.0 26.032194 2 AACGCGG 55 9.4849995E-5 25.906153 34-35 ATAACGC 95 0.0012233029 25.009459 3 ACATGGG 4375 0.0 24.872265 3 CATGGGG 1340 0.0 24.819065 4 TATCAAC 6710 0.0 24.364614 2 ATCAACG 6775 0.0 24.127205 3 TCAACGC 6810 0.0 23.929804 4 CAACGCA 6825 0.0 23.879019 5 TAACGCG 50 0.0016537765 23.75 76-77 AACGCAG 7000 0.0 23.27676 6 GGTATCA 4030 0.0 22.879848 1 GAGTACT 3485 0.0 22.555683 12-13 AGTACTT 3715 0.0 22.054186 12-13 >>END_MODULE