FastQCFastQC Report
Thu 26 May 2016
SRR937641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662529
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70521.0644062373118761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.8891686250715063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44560.6725743325952525No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.3294950107844336No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.20029311924459153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.18685974500738836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11890.17946384233746748No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.17236981324591075No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10610.160143933322164No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6880.10384451095725622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG502.8132496E-547.535461
TACCTGG5850.030.8544312
GTACCTG6100.030.3915251
GGTATCA44700.030.20151
TAACGCA804.4918375E-429.687244
GTATCAA63400.027.366631
GTACATG36750.025.6109431
TACATGG36900.024.5865042
ACATGGG36800.023.8788663
ACCTGGG7750.022.6772213
AGTACTT50100.022.65948312-13
ATCAACG77000.022.392663
GAGTACT47800.022.35859112-13
TATCAAC77700.022.2520582
CATGGGA24800.022.2175484
TCAACGC77650.022.2052154
CAACGCA78350.022.1280785
AACGCAG79850.021.7702376
GTATAGG2205.5859346E-821.607031
GTAGTAC1100.002876238421.5907213
Produced by FastQC (version 0.11.5)