##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937641_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662529 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.285149782122744 33.0 31.0 34.0 31.0 34.0 2 32.47478827341897 34.0 31.0 34.0 31.0 34.0 3 32.533003083638604 34.0 31.0 34.0 31.0 34.0 4 36.13397602218167 37.0 35.0 37.0 35.0 37.0 5 36.07478012283236 37.0 35.0 37.0 35.0 37.0 6 36.1317195171834 37.0 35.0 37.0 35.0 37.0 7 36.09096356536846 37.0 35.0 37.0 35.0 37.0 8 36.06633370010973 37.0 35.0 37.0 35.0 37.0 9 37.76012370779242 39.0 37.0 39.0 35.0 39.0 10-11 37.69070787844758 39.0 37.0 39.0 35.0 39.0 12-13 37.58032478578296 39.0 37.0 39.0 35.0 39.0 14-15 38.7743162940792 40.0 38.0 41.0 35.0 41.0 16-17 38.749613979161666 40.0 38.0 41.0 35.0 41.0 18-19 38.780423951253454 40.0 38.0 41.0 35.0 41.0 20-21 38.69913618875551 40.0 38.0 41.0 34.0 41.0 22-23 38.63479334489509 40.0 38.0 41.0 34.0 41.0 24-25 38.637443794913125 40.0 38.0 41.0 34.0 41.0 26-27 38.45117421275144 40.0 38.0 41.0 34.0 41.0 28-29 38.38843884569581 40.0 38.0 41.0 34.0 41.0 30-31 38.30801896973566 40.0 37.5 41.0 34.0 41.0 32-33 38.09734743686691 40.0 37.0 41.0 34.0 41.0 34-35 37.89795465556979 40.0 37.0 41.0 33.0 41.0 36-37 37.705734390494605 40.0 37.0 41.0 33.0 41.0 38-39 37.551205305730015 40.0 36.5 41.0 33.0 41.0 40-41 37.34559770213832 40.0 36.0 41.0 33.0 41.0 42-43 37.078404115140614 40.0 36.0 41.0 32.5 41.0 44-45 36.69101201607779 40.0 36.0 41.0 31.0 41.0 46-47 36.209760629346036 39.5 35.5 41.0 30.0 41.0 48-49 35.75758796973415 39.0 35.0 41.0 28.5 41.0 50-51 35.463763850337116 39.0 35.0 41.0 27.0 41.0 52-53 35.2003097222914 39.0 35.0 40.5 26.0 41.0 54-55 34.98081065130734 39.0 35.0 40.0 25.5 41.0 56-57 34.72533277788595 38.5 35.0 40.0 24.5 41.0 58-59 34.47704704246908 38.0 34.0 40.0 24.0 41.0 60-61 34.14285336943741 38.0 34.0 40.0 22.5 41.0 62-63 33.74113661439726 37.5 34.0 40.0 21.5 41.0 64-65 33.36118720840899 37.0 33.0 40.0 19.5 41.0 66-67 32.946465739612904 36.5 33.0 39.0 18.5 41.0 68-69 32.484301819241125 36.0 32.0 39.0 14.5 40.0 70-71 32.07420429898163 35.5 32.0 38.5 11.5 40.0 72-73 31.59762893397874 35.0 31.0 38.0 8.0 40.0 74-75 31.121511662131013 35.0 31.0 37.0 7.0 39.0 76-77 29.841966917674547 33.5 29.5 36.0 3.5 38.5 78-79 30.428511053855757 34.0 31.0 36.0 2.0 39.0 80-81 30.230278976467446 34.0 31.0 36.0 2.0 37.5 82-83 29.827332086595455 34.0 30.0 35.5 2.0 37.0 84-85 29.39877122359927 34.0 30.0 35.0 2.0 37.0 86-87 29.046802479589573 34.0 29.0 35.0 2.0 36.0 88-89 28.634822022885036 34.0 29.0 35.0 2.0 36.0 90-91 28.13316473693982 34.0 29.0 35.0 2.0 35.5 92-93 27.68815327329068 34.0 27.5 35.0 2.0 35.0 94-95 27.211888838073502 33.0 26.5 35.0 2.0 35.0 96-97 26.71122018809743 33.0 25.5 35.0 2.0 35.0 98-99 25.929693643598995 33.0 24.5 34.5 2.0 35.0 100-101 23.550791738927654 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36209260591367354 1101 2 0.34473637954904746 1101 3 0.40037257595590603 1101 4 0.2255593402028495 1101 5 0.1046747598018456 1101 6 0.24757199461301127 1101 7 0.09162550838778571 1101 8 0.23813231419201486 1101 9 0.2672047273144571 1101 10-11 0.2777259591175394 1101 12-13 0.2427346071392691 1101 14-15 0.33444533978990876 1101 16-17 0.44414361382477097 1101 18-19 0.4498726166050844 1101 20-21 0.44792116949963656 1101 22-23 0.502169755083429 1101 24-25 0.37361599982636307 1101 26-27 0.4082884082830063 1101 28-29 0.40856197583426734 1101 30-31 0.501670488090177 1101 32-33 0.3804249978795866 1101 34-35 0.35983203046399836 1101 36-37 0.3884527887586984 1101 38-39 0.4338551385711611 1101 40-41 0.22990181579317692 1101 42-43 0.48915512128072436 1101 44-45 0.4028855179534432 1101 46-47 0.6622086673240375 1101 48-49 0.39288064746670415 1101 50-51 0.669104009649871 1101 52-53 0.6138120674842824 1101 54-55 0.6944228757103232 1101 56-57 0.5958542473645778 1101 58-59 0.613374836067571 1101 60-61 0.5348659362632873 1101 62-63 0.6948082011827168 1101 64-65 0.7028204744667548 1101 66-67 0.8419112296478133 1101 68-69 0.8333321810596956 1101 70-71 0.49777475437442575 1101 72-73 0.6678078194652919 1101 74-75 0.6313794440702409 1101 76-77 0.6756064250796072 1101 78-79 0.7503723540483058 1101 80-81 0.9031088160217138 1101 82-83 0.7390514721292512 1101 84-85 0.9262396792362573 1101 86-87 1.0385973090579732 1101 88-89 1.0278276101484956 1101 90-91 0.8894193442619205 1101 92-93 1.1020548336943428 1101 94-95 1.2197202228288404 1101 96-97 1.1779883319208508 1101 98-99 1.300043110640587 1101 100-101 1.4835263885806391 1102 1 0.28932944801893257 1102 2 0.2313153269174677 1102 3 0.23530678648221937 1102 4 0.20996723493968972 1102 5 0.13776686506500369 1102 6 0.24803252092880257 1102 7 0.2724149820720001 1102 8 0.2497112615604351 1102 9 0.3014152536302461 1102 10-11 0.3917720117491186 1102 12-13 0.4032938176655847 1102 14-15 0.3759256029478024 1102 16-17 0.4513804559300283 1102 18-19 0.41230682713140254 1102 20-21 0.2853224852891074 1102 22-23 0.440656597188692 1102 24-25 0.39151073666847225 1102 26-27 0.4249331451251166 1102 28-29 0.42948302846584596 1102 30-31 0.43505864598490973 1102 32-33 0.40341841893221897 1102 34-35 0.511246504148211 1102 36-37 0.5298343677060657 1102 38-39 0.6673090859395785 1102 40-41 0.5142110263194937 1102 42-43 0.7038919633859919 1102 44-45 0.8569315705850187 1102 46-47 0.8958270883766701 1102 48-49 0.8833082790456572 1102 50-51 0.8178211149130306 1102 52-53 0.8350620674842801 1102 54-55 0.9536334020261066 1102 56-57 0.8757226684172039 1102 58-59 1.0187037834359884 1102 60-61 0.9690106731053874 1102 62-63 1.0550055696037646 1102 64-65 1.2921954744667588 1102 66-67 1.1481612296478048 1102 68-69 1.1795821810596934 1102 70-71 1.1488931754270553 1102 72-73 1.0218867668337133 1102 74-75 1.2120702335439262 1102 76-77 1.0908037935006583 1102 78-79 1.0051091961535654 1102 80-81 1.1078456581269762 1102 82-83 1.285202787918724 1102 84-85 1.159660731867838 1102 86-87 0.9291565195842857 1102 88-89 1.1314789259379694 1102 90-91 1.1249127653145514 1102 92-93 1.3531074652732862 1102 94-95 1.4540952228288369 1102 96-97 1.4040409634997957 1102 98-99 1.415273373798481 1102 100-101 1.9852698096332695 1103 1 0.24060137784349678 1103 2 0.35023570478521293 1103 3 0.30877338567250234 1103 4 0.17616662765224333 1103 5 0.237063423769456 1103 6 0.21511585426213742 1103 7 0.2077186257966943 1103 8 0.035129615136682446 1103 9 0.3296878582186409 1103 10-11 0.2435491709933828 1103 12-13 0.24740734668042563 1103 14-15 0.29100741806250596 1103 16-17 0.28852620491787917 1103 18-19 0.23236249514759777 1103 20-21 0.15662562294092908 1103 22-23 0.26529391162864613 1103 24-25 0.265451357451191 1103 26-27 0.25273847575939357 1103 28-29 0.39113619985585757 1103 30-31 0.4694167768890978 1103 32-33 0.5620714216312734 1103 34-35 0.4464885756731789 1103 36-37 0.36874968484507065 1103 38-39 0.491108849772246 1103 40-41 0.5389099129591699 1103 42-43 0.7233993857881487 1103 44-45 0.8128888917726087 1103 46-47 0.7907174392538607 1103 48-49 0.8128705260092133 1103 50-51 0.7759191243597172 1103 52-53 0.6910050499404221 1103 54-55 0.8316023628898037 1103 56-57 0.8299804282012815 1103 58-59 0.5192790196033243 1103 60-61 0.6683510914589661 1103 62-63 0.6822744629910815 1103 64-65 0.8258805284478612 1103 66-67 0.8233214860580702 1103 68-69 0.9928463511001837 1103 70-71 1.0426296801504051 1103 72-73 1.085975835659621 1103 74-75 0.9832350446100548 1103 76-77 0.9430322010580134 1103 78-79 0.9516712744261717 1103 80-81 1.0751061169663814 1103 82-83 1.3240211077567778 1103 84-85 1.0767963593982017 1103 86-87 1.3236006828771387 1103 88-89 1.3231886087989686 1103 90-91 1.5314740001323663 1103 92-93 1.5960663046794963 1103 94-95 1.5389509920596076 1103 96-97 1.6124181564822528 1103 98-99 1.5644830566594798 1103 100-101 1.681873217190624 1104 1 0.16040087659036573 1104 2 0.200003373564698 1104 3 0.10838623254635849 1104 4 0.127784290333274 1104 5 -0.0016318229816434382 1104 6 0.11064549468973439 1104 7 0.043202154083658684 1104 8 0.2231369175371114 1104 9 0.23829572010254907 1104 10-11 0.046365670641250745 1104 12-13 0.044079167519811335 1104 14-15 0.13117888283117907 1104 16-17 0.18527258420991188 1104 18-19 0.22086003412848498 1104 20-21 0.14372445321913574 1104 22-23 0.14450134937528958 1104 24-25 0.11912006611452597 1104 26-27 0.2716131742796293 1104 28-29 0.26681538998187904 1104 30-31 0.12238554102864185 1104 32-33 0.21350770464650282 1104 34-35 0.14905444875461882 1104 36-37 0.18073451347866154 1104 38-39 0.15953029585212164 1104 40-41 0.19316328579471076 1104 42-43 0.31603482052884857 1104 44-45 0.35567245979785156 1104 46-47 0.4439065344408064 1104 48-49 0.48343036359376157 1104 50-51 0.5603192927555938 1104 52-53 0.5601677526154774 1104 54-55 0.6561844224342721 1104 56-57 0.43451275588076754 1104 58-59 0.47986631987913597 1104 60-61 0.4696921599858541 1104 62-63 0.3346520710614911 1104 64-65 0.4508161012889147 1104 66-67 0.7243456319801638 1104 68-69 0.5938278078818584 1104 70-71 0.5069871987506716 1104 72-73 0.7420616939474165 1104 74-75 0.7150567495789133 1104 76-77 0.5810479625968696 1104 78-79 0.8221864556287848 1104 80-81 0.4526561537538001 1104 82-83 0.28525563048431835 1104 84-85 0.32290417210107236 1104 86-87 0.5744170880974089 1104 88-89 0.45872564605458876 1104 90-91 0.6495793105040555 1104 92-93 0.7787561533199359 1104 94-95 0.7901283860941462 1104 96-97 0.7761182229750148 1104 98-99 1.2532270909996441 1104 100-101 1.3016674043854586 1105 1 0.1487623553671753 1105 2 0.10403098186954196 1105 3 0.11726764910203258 1105 4 0.12034662791093353 1105 5 0.1140408267263453 1105 6 0.11341191390004468 1105 7 0.15765459868541853 1105 8 0.17634065453167835 1105 9 0.24502547727242074 1105 10-11 0.19095531845837854 1105 12-13 0.18610731606813857 1105 14-15 0.20230938889028494 1105 16-17 0.41615681375750313 1105 18-19 0.37794979837740783 1105 20-21 0.34279253321243175 1105 22-23 0.14187464830691 1105 24-25 0.16106345232341823 1105 26-27 0.12556812915067184 1105 28-29 0.16160762910482873 1105 30-31 0.2037387578060006 1105 32-33 0.18977424960313982 1105 34-35 0.22438348497887972 1105 36-37 0.067921428408944 1105 38-39 0.03835740862328407 1105 40-41 -0.036270203712462035 1105 42-43 -0.11858407495267187 1105 44-45 -0.20453208756194385 1105 46-47 -0.21334623430703914 1105 48-49 -0.23395010753581147 1105 50-51 -0.1948526071317005 1105 52-53 -0.029638411748940996 1105 54-55 -0.01769247656813633 1105 56-57 -0.06752014308439414 1105 58-59 -0.22844877247455742 1105 60-61 0.09407856447919016 1105 62-63 0.05015333318203119 1105 64-65 -0.05616514853644361 1105 66-67 -0.03213030389851923 1105 68-69 -0.01858475184764785 1105 70-71 -0.1536188054036174 1105 72-73 0.08680689462924107 1105 74-75 0.19020322076437424 1105 76-77 0.10315602992238482 1105 78-79 0.18061239104174476 1105 80-81 0.2973664249001402 1105 82-83 0.33859535980370836 1105 84-85 0.6654674411330106 1105 86-87 0.5488230531306151 1105 88-89 0.7182660665130491 1105 90-91 0.8197350496596023 1105 92-93 0.5848087432285602 1105 94-95 0.6491131940748431 1105 96-97 0.8693684395381354 1105 98-99 0.9355988530317063 1105 100-101 1.0959906562786372 1106 1 -0.5752313073176829 1106 2 -0.5094400096999365 1106 3 -0.43361973240119767 1106 4 -0.43476388164487645 1106 5 -0.303146517529413 1106 6 -0.5303685463946763 1106 7 -0.4501794350051824 1106 8 -0.26054325403890743 1106 9 -0.7535108547441354 1106 10-11 -0.7642388667402074 1106 12-13 -0.8740368473590436 1106 14-15 -0.9752351113379163 1106 16-17 -0.9124536984213663 1106 18-19 -1.0445466211444625 1106 20-21 -0.6989416772724724 1106 22-23 -1.0139718921380663 1106 24-25 -0.9074876212954095 1106 26-27 -0.9857257218699615 1106 28-29 -0.8551393032254495 1106 30-31 -0.8045606068886499 1106 32-33 -1.2134216878001283 1106 34-35 -1.4858522232737883 1106 36-37 -1.3592440395188916 1106 38-39 -1.4972506431244597 1106 40-41 -1.4872596222847818 1106 42-43 -1.9376597607519486 1106 44-45 -1.8418912947680255 1106 46-47 -1.7384935191767 1106 48-49 -2.075783469722815 1106 50-51 -2.169012834183853 1106 52-53 -2.2075344021380445 1106 54-55 -2.403222098794906 1106 56-57 -2.8834809440622706 1106 58-59 -2.856072489142008 1106 60-61 -2.6168247127730986 1106 62-63 -2.8442042005111787 1106 64-65 -3.6125530887516355 1106 66-67 -3.6301331053275625 1106 68-69 -3.4215118255084462 1106 70-71 -3.4286047720277892 1106 72-73 -3.3861427898165353 1106 74-75 -3.1953014823838224 1106 76-77 -3.080893661343346 1106 78-79 -2.9801576347167114 1106 80-81 -3.251333323810627 1106 82-83 -3.4068877293226585 1106 84-85 -3.503676706555808 1106 86-87 -3.2628621585766346 1106 88-89 -3.396272510843641 1106 90-91 -3.835441298724856 1106 92-93 -4.056839168552656 1106 94-95 -4.036519514445381 1106 96-97 -3.795109282805619 1106 98-99 -3.993237504690846 1106 100-101 -3.6285879489874198 1107 1 0.15456479437772686 1107 2 0.10193699654446675 1107 3 0.060335649714900796 1107 4 0.1285040910675761 1107 5 0.03387920964760127 1107 6 0.10735978558244597 1107 7 -0.005418055228183505 1107 8 -0.005555168279705924 1107 9 0.05248097299081422 1107 10-11 -0.05895956018693482 1107 12-13 0.09499341802082739 1107 14-15 0.048997316979772165 1107 16-17 0.07389377742203607 1107 18-19 0.23434723565716098 1107 20-21 0.152141979072411 1107 22-23 0.2147129915048609 1107 24-25 0.2225409320503502 1107 26-27 0.317448686865788 1107 28-29 0.2139501687855656 1107 30-31 0.18828355717496237 1107 32-33 0.33720727328390154 1107 34-35 0.3175175965105481 1107 36-37 0.39969893253732636 1107 38-39 0.5383914571651616 1107 40-41 0.38640130163032893 1107 42-43 0.2120003115209741 1107 44-45 0.20503214784967128 1107 46-47 0.31947939743528053 1107 48-49 0.43717928259805205 1107 50-51 0.5310793298330978 1107 52-53 0.49764865718233153 1107 54-55 0.3456849117952032 1107 56-57 0.6368505547404695 1107 58-59 0.7668454353010006 1107 60-61 0.5666283462847872 1107 62-63 0.6927386957138921 1107 64-65 0.6101250925839778 1107 66-67 0.6157567003405262 1107 68-69 0.6594423053936254 1107 70-71 0.5232293210753696 1107 72-73 0.5850661629260792 1107 74-75 0.7938852868298945 1107 76-77 0.6492918041578548 1107 78-79 0.67572642528323 1107 80-81 0.7061049831713824 1107 82-83 0.695825567670056 1107 84-85 0.6666633961662356 1107 86-87 0.797916510703292 1107 88-89 0.7558272829539519 1107 90-91 0.717849044177786 1107 92-93 0.8844529359660065 1107 94-95 1.1507148942320313 1107 96-97 1.2393384768212883 1107 98-99 1.3843330984876445 1107 100-101 1.5719694099885082 1108 1 -0.09409647790698727 1108 2 -0.007943932341788695 1108 3 0.04720493463037201 1108 4 0.12945797568043105 1108 5 -0.13440906086091786 1108 6 -0.0015508124045311433 1108 7 0.08056313022014194 1108 8 0.04100755785673016 1108 9 0.02734117955617421 1108 10-11 -0.0770011363990264 1108 12-13 -0.05256266381589825 1108 14-15 -0.04613458223738576 1108 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0.1185344520778493 2311 96-97 -0.4993286017175933 2311 98-99 -0.3801836420117972 2311 100-101 0.8041161535067864 2312 1 0.05284112117068673 2312 2 0.027969023415522543 2312 3 -0.04958134581350038 2312 4 -0.07431778451556426 2312 5 0.062139433158392876 2312 6 -0.034559891522562225 2312 7 -0.055911866177027036 2312 8 -0.051397687855903484 2312 9 0.1483024131633215 2312 10-11 0.1907968171965848 2312 12-13 0.01452193050604933 2312 14-15 0.1933307585898234 2312 16-17 0.019571117408631267 2312 18-19 -0.022843951078719726 2312 20-21 0.11292219346031374 2312 22-23 0.04603111080737676 2312 24-25 -0.02024023609417469 2312 26-27 0.059519721000604875 2312 28-29 0.24580598566429757 2312 30-31 0.19526049423394198 2312 32-33 0.04355217379603715 2312 34-35 0.010689597533421136 2312 36-37 0.22338008755042438 2312 38-39 0.20930568127032245 2312 40-41 0.21749653215606912 2312 42-43 0.3090865159151761 2312 44-45 0.18400112311420713 2312 46-47 -0.018535752090258484 2312 48-49 -0.03485076764695094 2312 50-51 0.17365039506866964 2312 52-53 0.2963023398578244 2312 54-55 0.26793301292105554 2312 56-57 0.211656520557284 2312 58-59 0.16586818032315165 2312 60-61 0.0507859649341853 2312 62-63 0.23625556960376315 2312 64-65 0.39615705034224646 2312 66-67 0.3470668716711529 2312 68-69 0.33586136393908106 2312 70-71 0.737117883598259 2312 72-73 0.5490462999076406 2312 74-75 0.40993258762953033 2312 76-77 0.48903822929832685 2312 78-79 0.4000216475154339 2312 80-81 0.6174954635744463 2312 82-83 0.6659688385023799 2312 84-85 0.6587171521012998 2312 86-87 0.8739060332029673 2312 88-89 0.9160290232142323 2312 90-91 0.8181593606452928 2312 92-93 0.9027280878413819 2312 94-95 1.0848953201051046 2312 96-97 1.3481168389861793 2312 98-99 1.5289188018140436 2312 100-101 1.9311866384270466 2313 1 -0.22581327757959002 2313 2 -0.10430161599229137 2313 3 0.10126673123360064 2313 4 -0.07260873191113859 2313 5 0.06674706764326999 2313 6 -0.04455265402516062 2313 7 -0.12467525733868712 2313 8 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0.8875234700863572 2313 72-73 1.1964171535740427 2313 74-75 1.0116086773744968 2313 76-77 1.158766500682983 2313 78-79 0.32372337663239037 2313 80-81 0.6410316618102172 2313 82-83 0.5064608450089629 2313 84-85 0.7415990375768615 2313 86-87 0.6210522378163326 2313 88-89 0.9834506110208423 2313 90-91 0.7157702699186039 2313 92-93 0.9378174100246675 2313 94-95 1.2687994125157616 2313 96-97 1.503714075838655 2313 98-99 1.9300857586971922 2313 100-101 2.451935325287046 2314 1 -0.1898787370344479 2314 2 -0.421994281623455 2314 3 -0.26072969038610694 2314 4 -0.5845923736012324 2314 5 -0.4250311776396245 2314 6 -0.12711427622422633 2314 7 -0.07470245565042433 2314 8 -0.13713927224739564 2314 9 -0.12580894565800804 2314 10-11 -0.020489998927040176 2314 12-13 0.0365700632171837 2314 14-15 0.09581216878409293 2314 16-17 -0.00831705296676688 2314 18-19 -0.2603399700216258 2314 20-21 -0.06521799513794235 2314 22-23 0.027600547366631645 2314 24-25 0.030976928839287154 2314 26-27 0.13197496007173726 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0.6745754368035399 2314 94-95 0.45032520503524154 2314 96-97 0.5133203229304044 2314 98-99 0.2113654556490161 2314 100-101 0.5340086886368276 2315 1 -0.16753157434528276 2315 2 -0.14836518426464096 2315 3 -0.24375471511522306 2315 4 -0.010643787424527318 2315 5 -0.05848313493499546 2315 6 -0.13738680814468296 2315 7 -0.19378310099509832 2315 8 -0.061678514797392836 2315 9 -0.016955353398508066 2315 10-11 -0.03668046428921912 2315 12-13 -0.039931022590003806 2315 14-15 -0.0013647325154551027 2315 16-17 -0.003970981769647608 2315 18-19 0.19344500604514536 2315 20-21 -0.04219149234667441 2315 22-23 -0.0330454794886208 2315 24-25 -0.0482017233954295 2315 26-27 0.07684608442223606 2315 28-29 0.06176737351377426 2315 30-31 0.11987294310951313 2315 32-33 -0.5730053031125095 2315 34-35 -0.31635133930227255 2315 36-37 -0.15092841184664252 2315 38-39 0.005609804789422412 2315 40-41 0.03162117008946552 2315 42-43 -0.15612401105490648 2315 44-45 0.0015381999779933153 2315 46-47 0.16764418102842882 2315 48-49 0.5120562822405432 2315 50-51 0.42016336730919335 2315 52-53 0.614930278346904 2315 54-55 0.4831062454765842 2315 56-57 0.48359806777822456 2315 58-59 0.40893141921873877 2315 60-61 0.6428006092076259 2315 62-63 0.7706006175270872 2315 64-65 0.7082118482686681 2315 66-67 0.3951859900311945 2315 68-69 0.4981884110916468 2315 70-71 0.5684259230308868 2315 72-73 0.654698268431158 2315 74-75 0.5847719108602227 2315 76-77 0.5991624196987431 2315 78-79 0.6430724549395102 2315 80-81 0.6376220159544523 2315 82-83 0.6453046249794276 2315 84-85 0.9625920417719911 2315 86-87 0.998503564312724 2315 88-89 1.1120360345641664 2315 90-91 0.8540800975829228 2315 92-93 0.9586106601614652 2315 94-95 1.0101355582920988 2315 96-97 1.3917326567905306 2315 98-99 1.6776116325844228 2315 100-101 1.9042973655438153 2316 1 -0.26116775086061494 2316 2 -0.008908762718384367 2316 3 -0.11718971211721652 2316 4 -0.080470440130334 2316 5 -0.22234868115348405 2316 6 -0.11121467795074835 2316 7 -0.10259902353024586 2316 8 -0.04828593731911468 2316 9 -0.20516737942297425 2316 10-11 -0.21736839441334155 2316 12-13 -0.036067176732174744 2316 14-15 -0.18321830461522381 2316 16-17 -0.23902570653495303 2316 18-19 -0.2640482148853991 2316 20-21 -0.015081926475602359 2316 22-23 -0.11268724034631816 2316 24-25 -0.019749767533213003 2316 26-27 -0.3881936055751609 2316 28-29 -0.2377578678926895 2316 30-31 -0.08018119995346495 2316 32-33 -0.2178648443731035 2316 34-35 -0.2022146303055692 2316 36-37 -0.125302186915782 2316 38-39 -0.06908972358422716 2316 40-41 -0.03359884763008836 2316 42-43 -0.07822288255238874 2316 44-45 0.022181920725074633 2316 46-47 -0.028168009662543625 2316 48-49 0.20990666840139482 2316 50-51 0.20370521855448942 2316 52-53 0.0988260730865278 2316 54-55 0.3164485280765277 2316 56-57 0.3675574023107586 2316 58-59 0.30496778903822985 2316 60-61 0.2649245386516128 2316 62-63 0.2789726564384978 2316 64-65 0.14032047446675477 2316 66-67 0.14954428286909405 2316 68-69 0.2952369429644577 2316 70-71 0.09510816142145018 2316 72-73 -0.0860123928301526 2316 74-75 0.02695293662515752 2316 76-77 0.2875019727723682 2316 78-79 0.012791268982699222 2316 80-81 -0.11770056036041865 2316 82-83 -0.47727795437819864 2316 84-85 -0.09592050062516222 2316 86-87 -0.1776694608078664 2316 88-89 0.03082591473348728 2316 90-91 0.08738300340979421 2316 92-93 0.45605564454499614 2316 94-95 0.2802244245095089 2316 96-97 0.8343700951524582 2316 98-99 0.5781427715575838 2316 100-101 1.0011329048713655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 7.0 5 18.0 6 41.0 7 87.0 8 233.0 9 419.0 10 681.0 11 1202.0 12 1965.0 13 2886.0 14 3663.0 15 4989.0 16 6923.0 17 10410.0 18 9400.0 19 5332.0 20 3998.0 21 3982.0 22 3961.0 23 4204.0 24 4562.0 25 5322.0 26 6111.0 27 7423.0 28 9146.0 29 11138.0 30 13663.0 31 17175.0 32 21857.0 33 28668.0 34 40899.0 35 59313.0 36 90174.0 37 146016.0 38 125358.0 39 11300.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.912398089465334 19.849959907765207 15.566206549757409 20.67143545301205 2 15.05096380686732 21.34880133548871 41.4846746331104 22.115560224533567 3 19.239912517036988 26.846070134288464 31.063093087245992 22.850924261428556 4 12.019398396145679 17.754694511485535 38.37039586191699 31.8555112304518 5 12.127332309917024 38.565285054650126 34.63207579922323 14.675306836209618 6 29.044615405514325 37.71774518549377 18.385912163844903 14.851727245147007 7 26.51446200845548 34.63033316277476 21.475890111980004 17.379314716789757 8 25.94316626140139 35.22351474426026 20.213907617628813 18.619411376709547 9 26.457860712512204 16.954125781663897 21.377781199011668 35.21023230681223 10-11 24.08656828606748 27.833272203933713 27.595093950604426 20.485065559394382 12-13 25.702384779914656 25.434231003195507 28.811625179435858 20.05175903745398 14-15 22.31366476033502 26.236813784755082 28.64765164996551 22.80186980494439 16-17 19.936938609479736 29.670021991490188 29.1617423539196 21.231297045110477 18-19 19.732419260138045 27.360840053793872 33.26978894508768 19.636951740980397 20-21 20.63677260676333 26.214494923614605 33.60534678885512 19.54338568076694 22-23 20.27304733046802 26.236995724673502 33.623235437287036 19.86672150757144 24-25 20.116628857000975 26.39280695637474 33.93126942367806 19.559294762946227 26-27 20.107241236179767 26.452586694841706 33.86204294177578 19.578129127202747 28-29 19.634608084416882 26.741169909202945 34.18153222197317 19.442689784406998 30-31 19.940712029209287 26.55393197882659 33.93904266832094 19.566313323643193 32-33 19.779058727995302 26.75188557783886 33.675884376382015 19.793171317783827 34-35 20.116628857000975 26.55921476644796 33.60532142743941 19.71883494911166 36-37 20.196248753823433 26.364002043691837 33.77012089327748 19.669628309207255 38-39 20.00470922782248 26.81935432260324 33.74674919890299 19.429187250671294 40-41 20.018263392840296 26.80537788997438 33.67354562640796 19.502813090777362 42-43 19.974637585437748 26.804599956974474 33.25291837605063 19.96784408153714 44-45 20.000860339698338 26.9441790472568 32.85773151062067 20.197229102424195 46-47 20.267641114577625 27.28461697525693 32.3140571959869 20.13368471417855 48-49 20.264018631637256 27.628677386197438 31.827889798031485 20.279414184133827 50-51 20.474198110573273 27.781802758822632 31.545260660288076 20.198738470316016 52-53 20.472990616259818 28.23423578439585 31.106110072162878 20.18666352718145 54-55 20.310054352337783 27.969719061354297 31.270027425214597 20.450199161093327 56-57 20.532384242802955 28.17635152574453 30.962040906888603 20.329223324563905 58-59 20.611324183545175 27.91711759032435 31.077884892585832 20.393673333544644 60-61 20.51887540017116 28.00020829276907 31.585334377815915 19.895581929243853 62-63 20.611399651939763 28.00662310630931 31.372362568280032 20.009614673470896 64-65 20.480990266086465 27.928135975934637 31.418549225769738 20.172324532209156 66-67 20.21217184455322 28.188124595300735 31.151164703733723 20.448538856412323 68-69 20.28394228780929 28.128957373941365 31.14331599069626 20.443784347553088 70-71 20.4916313097238 28.201331564354167 30.914571286690844 20.39246583923119 72-73 20.335789074893327 28.078997296722108 30.9149486286638 20.670264999720768 74-75 20.315261671564567 28.623275358512608 30.380707863353905 20.68075510656892 76-77 20.249453231481187 28.22163256249915 30.802274315539396 20.726639890480268 78-79 20.197681912791744 28.119146482644535 30.884534865643616 20.798636738920106 80-81 20.24952869987578 28.50697856244783 30.585453617879367 20.65803911979702 82-83 20.357297567351768 28.39090817156683 30.71382535707871 20.537968904002692 84-85 20.262011567812984 28.330878091190108 30.472447956916536 20.934662384080372 86-87 20.165532376695964 28.29068614354994 30.559341553350873 20.984439926403223 88-89 20.299413308700448 28.01975460696815 30.52892779033069 21.151904294000715 90-91 20.038537099694555 27.99841806186918 30.932928389374148 21.03011644906212 92-93 20.275660226846494 28.03499072101383 30.676879211674844 21.01246984046483 94-95 19.893317877406123 27.998397051298884 30.67420444991842 21.434080621376577 96-97 19.890072736438707 28.061639565966168 30.696618563111954 21.35166913448317 98-99 19.9656919383602 28.17577862923219 30.306108510376568 21.552420922031043 100-101 19.5748554245785 28.10650351645465 30.900571659745463 21.418069399221384 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 898.0 1 666.0 2 1075.5 3 1464.0 4 901.5 5 584.5 6 910.5 7 1846.5 8 2739.0 9 3035.5 10 2599.0 11 2035.5 12 1971.0 13 2038.5 14 2076.5 15 2172.5 16 2254.5 17 2259.5 18 2308.5 19 2392.0 20 2491.0 21 2706.0 22 3030.0 23 3503.0 24 4061.5 25 4758.5 26 5637.5 27 6688.5 28 7713.5 29 8830.5 30 9973.5 31 10982.0 32 12266.0 33 13626.0 34 15355.5 35 17053.5 36 18888.0 37 20583.5 38 21379.5 39 21668.0 40 22137.5 41 22875.5 42 23035.0 43 23715.5 44 24792.0 45 25316.0 46 25007.0 47 25172.5 48 27677.5 49 26779.5 50 23022.0 51 20640.5 52 18155.0 53 16516.0 54 15257.5 55 13495.5 56 11669.5 57 10025.5 58 8553.5 59 7353.0 60 6065.5 61 4822.0 62 3698.5 63 2680.0 64 1944.5 65 1404.5 66 1012.0 67 774.5 68 584.5 69 379.5 70 247.5 71 177.5 72 149.0 73 122.0 74 95.5 75 72.5 76 53.0 77 28.0 78 13.5 79 11.0 80 7.0 81 4.5 82 3.5 83 3.0 84 1.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04618665748970988 2 0.0 3 0.0 4 0.0 5 0.006339345145646455 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005433724410554105 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.546839459102923E-5 22-23 9.8108912968338E-4 24-25 0.0 26-27 6.037471567282339E-4 28-29 0.0011320259188654384 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.7734197295514614E-4 38-39 0.0 40-41 2.2640518377308765E-4 42-43 0.019848187777440685 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.5093678918205847E-4 86-87 0.0 88-89 0.0 90-91 0.0076223078536939515 92-93 0.0015848362864116137 94-95 0.0 96-97 0.0 98-99 3.7734197295514614E-4 100-101 0.01086744882110821 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 662529.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.01396471329126 #Duplication Level Percentage of deduplicated Percentage of total 1 84.49823320726401 54.93565152072518 2 9.5346862639601 12.397755126348098 3 2.529401536865225 4.933392667905013 4 1.0446662031308362 2.716715666700646 5 0.5860817606091913 1.905174945167479 6 0.3532017557779187 1.377782789209087 7 0.245574768434159 1.1176052530617162 8 0.1819053242857688 0.9461109067419822 9 0.1304303931504587 0.7631817280042164 >10 0.8189031033496089 9.724763443815167 >50 0.05112085521860382 2.2548419499997285 >100 0.02251184985100802 2.5818547293017406 >500 0.0011724921797067287 0.4515768354333265 >1k 0.00164148905158942 1.9203449760696083 >5k 4.6899687188269143E-4 1.9732474615170643 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7052 1.0644062373118761 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 0.8891686250715063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4456 0.6725743325952525 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2183 0.3294950107844336 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1327 0.20029311924459153 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1238 0.18685974500738836 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1189 0.17946384233746748 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1142 0.17236981324591075 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1061 0.160143933322164 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 688 0.10384451095725622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5093678918205844E-4 0.0 5 0.0 0.0 0.0 1.5093678918205844E-4 0.0 6 0.0 0.0 0.0 1.5093678918205844E-4 0.0 7 1.5093678918205844E-4 0.0 0.0 1.5093678918205844E-4 0.0 8 1.5093678918205844E-4 0.0 0.0 1.5093678918205844E-4 0.0 9 1.5093678918205844E-4 0.0 0.0 1.5093678918205844E-4 0.0 10-11 1.5093678918205844E-4 0.0 0.0 1.5093678918205844E-4 0.0 12-13 1.5093678918205844E-4 0.0 0.0 1.5093678918205844E-4 0.0 14-15 1.5093678918205844E-4 0.0 0.0 3.018735783641169E-4 0.0 16-17 1.5093678918205844E-4 0.0 0.0 3.018735783641169E-4 0.0 18-19 2.2640518377308767E-4 0.0 0.0 3.018735783641169E-4 0.0 20-21 3.018735783641169E-4 0.0 0.0 3.018735783641169E-4 0.0 22-23 3.018735783641169E-4 0.0 0.0 0.0010565575242744091 0.0 24-25 3.018735783641169E-4 0.0 0.0 0.001660304681002643 0.0 26-27 3.018735783641169E-4 0.0 0.0 0.0021131150485488182 0.0 28-29 3.018735783641169E-4 0.0 0.0 0.003622482940369403 0.0 30-31 3.018735783641169E-4 0.0 0.0 0.006339345145646455 0.0 32-33 3.018735783641169E-4 0.0 0.0 0.0098108912968338 0.0 34-35 3.018735783641169E-4 0.0 0.0 0.012376816712928793 0.0 36-37 3.018735783641169E-4 0.0 0.0 0.01622570483707128 0.0 38-39 3.018735783641169E-4 0.0 0.0 0.021885834431398472 0.0 40-41 3.018735783641169E-4 0.0 0.0 0.027923305998680814 0.0 42-43 5.282787621372046E-4 0.0 0.0 0.039771843949472405 0.0 44-45 6.037471567282338E-4 0.0 0.0 0.05395990213258589 0.0 46-47 6.037471567282338E-4 0.0 0.0 0.06626125045092365 0.0 48-49 6.037471567282338E-4 0.0 0.0 0.08301523405013214 0.0 50-51 6.037471567282338E-4 0.0 0.0 0.10173139590870739 0.0 52-53 6.037471567282338E-4 0.0 0.0 0.12920189153984202 0.0 54-55 6.037471567282338E-4 0.0 0.0 0.14837086376596345 0.0 56-57 6.037471567282338E-4 0.0 0.0 0.1968215730934042 0.0 58-59 6.037471567282338E-4 0.0 0.0 0.25968674578773154 0.0 60-61 6.037471567282338E-4 0.0 0.0 0.32187270293073966 0.0 62-63 6.037471567282338E-4 0.0 0.0 0.4175666272721647 0.0 64-65 6.037471567282338E-4 0.0 0.0 0.48133742070158436 0.0 66-67 6.037471567282338E-4 0.0 0.0 0.596426722452904 0.0 68-69 6.037471567282338E-4 0.0 0.0 0.7326471746897116 0.0 70-71 6.79215551319263E-4 0.0 0.0 0.836718090830741 0.0 72-73 7.546839459102922E-4 0.0 0.0 0.9943715671314011 0.0 74-75 8.301523405013214E-4 0.0 0.0 1.1668168487719028 0.0 76-77 9.056207350923507E-4 0.0 0.0 1.293377346501059 0.0 78-79 9.056207350923507E-4 0.0 0.0 1.423333921986811 0.0 80-81 9.056207350923507E-4 0.0 0.0 1.63736228904697 0.0 82-83 9.056207350923507E-4 0.0 0.0 1.8278445169947277 0.0 84-85 9.056207350923507E-4 0.0 0.0 2.081342854425995 0.0 86-87 9.056207350923507E-4 0.0 0.0 2.422912808344993 0.0 88-89 9.056207350923507E-4 0.0 0.0 2.8656858794105613 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 50 2.8132496E-5 47.53546 1 TACCTGG 585 0.0 30.854431 2 GTACCTG 610 0.0 30.391525 1 GGTATCA 4470 0.0 30.2015 1 TAACGCA 80 4.4918375E-4 29.68724 4 GTATCAA 6340 0.0 27.36663 1 GTACATG 3675 0.0 25.610943 1 TACATGG 3690 0.0 24.586504 2 ACATGGG 3680 0.0 23.878866 3 ACCTGGG 775 0.0 22.677221 3 AGTACTT 5010 0.0 22.659483 12-13 ATCAACG 7700 0.0 22.39266 3 GAGTACT 4780 0.0 22.358591 12-13 TATCAAC 7770 0.0 22.252058 2 CATGGGA 2480 0.0 22.217548 4 TCAACGC 7765 0.0 22.205215 4 CAACGCA 7835 0.0 22.128078 5 AACGCAG 7985 0.0 21.770237 6 GTATAGG 220 5.5859346E-8 21.60703 1 GTAGTAC 110 0.0028762384 21.590721 3 >>END_MODULE