FastQCFastQC Report
Thu 26 May 2016
SRR937640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552058
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47400.8586054363853074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37380.6771027681874006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.48273913248245653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.2543211039419771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10030.18168380858532981No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9780.1771552988997533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.14726713497494828No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.13839125599121832No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.13168906165656508No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6600.11955265569922001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG255.2533105E-576.0570765
TACCTGG5800.036.0615432
GTCCGGG550.002564417434.5839541
GTACCTG6350.034.44781
GTACACG753.0563492E-431.7019561
AGGCCGT600.00394519931.6731996
GTCCATA1354.177473E-728.179521
GTATCAA55500.027.9319951
TATAACG700.00835718427.1632442
GTACATG33550.026.7883911
TACATGG34050.026.2458342
GAGTACT32200.025.73976312-13
GTCATAC950.001217810225.0278631
GGTATCA39500.024.9201471
CAACGCA62750.024.8473325
TCAACGC62600.024.7549974
ATCAACG62700.024.7155173
ACCTGGG8300.024.6269153
TATCAAC62750.024.6200712
AACGCAG64000.024.3487726
Produced by FastQC (version 0.11.5)