##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937640_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 552058 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83840103757214 33.0 31.0 34.0 30.0 34.0 2 31.910212332762136 33.0 31.0 34.0 30.0 34.0 3 31.942585742802386 33.0 31.0 34.0 30.0 34.0 4 35.55312485282344 37.0 35.0 37.0 33.0 37.0 5 35.57629995399034 37.0 35.0 37.0 33.0 37.0 6 35.65214705701212 37.0 35.0 37.0 33.0 37.0 7 35.58399842045582 37.0 35.0 37.0 33.0 37.0 8 35.600799191389314 37.0 35.0 37.0 33.0 37.0 9 37.153076307199605 39.0 37.0 39.0 34.0 39.0 10-11 37.09206641331164 39.0 37.0 39.0 34.0 39.0 12-13 36.98724409391767 39.0 37.0 39.0 33.0 39.0 14-15 38.103259983552455 40.0 38.0 41.0 33.0 41.0 16-17 38.02913552561506 40.0 38.0 41.0 33.0 41.0 18-19 37.99978806574671 40.0 38.0 41.0 33.0 41.0 20-21 38.04878744624659 40.0 38.0 41.0 33.5 41.0 22-23 38.036207246340055 40.0 38.0 41.0 33.0 41.0 24-25 37.96195146162179 40.0 38.0 41.0 33.0 41.0 26-27 37.842567266482874 40.0 38.0 41.0 33.0 41.0 28-29 37.70039017639451 40.0 37.5 41.0 33.0 41.0 30-31 37.43552670190451 40.0 37.0 41.0 32.5 41.0 32-33 37.23876839027783 40.0 36.0 41.0 32.0 41.0 34-35 37.169724014505725 40.0 36.0 41.0 32.0 41.0 36-37 37.00322339319419 40.0 36.0 41.0 31.0 41.0 38-39 36.831262294903794 39.5 36.0 41.0 31.0 41.0 40-41 36.59584137898554 39.0 35.5 41.0 30.5 41.0 42-43 36.32345876701361 39.0 35.0 41.0 30.0 41.0 44-45 36.013484090439775 39.0 35.0 41.0 29.5 41.0 46-47 35.57614417325716 39.0 35.0 40.5 27.5 41.0 48-49 35.16465208365787 39.0 35.0 40.0 25.5 41.0 50-51 34.28199301522666 38.0 34.0 39.5 23.5 40.5 52-53 34.384638932865755 38.0 34.5 39.5 24.0 40.5 54-55 34.656857612787064 38.0 35.0 40.0 24.0 41.0 56-57 34.4462438729264 38.0 34.0 40.0 23.0 41.0 58-59 34.1738522039351 38.0 34.0 40.0 22.5 41.0 60-61 33.84142245923436 38.0 34.0 40.0 21.0 41.0 62-63 33.48835539019451 37.5 33.0 40.0 19.5 41.0 64-65 33.06916755123556 37.0 33.0 40.0 17.5 41.0 66-67 32.54980092671423 36.0 32.0 39.0 12.5 41.0 68-69 32.10266584308171 36.0 31.5 39.0 8.0 40.0 70-71 31.638985396461965 35.0 31.0 38.5 7.0 40.0 72-73 31.153789094624116 35.0 31.0 38.0 2.0 40.0 74-75 30.60311054273283 35.0 30.0 37.0 2.0 39.0 76-77 30.06104521626351 34.5 29.5 36.5 2.0 39.0 78-79 29.528007926703353 34.0 29.0 36.0 2.0 39.0 80-81 28.976869278228012 34.0 29.0 36.0 2.0 37.5 82-83 28.42779653587123 34.0 27.5 35.0 2.0 37.0 84-85 27.86650406297889 33.5 26.5 35.0 2.0 36.5 86-87 27.291694894377038 33.0 26.0 35.0 2.0 36.0 88-89 26.73170391516833 33.0 25.0 35.0 2.0 36.0 90-91 26.164654800763685 32.0 24.5 35.0 2.0 35.0 92-93 25.495150871828685 32.0 21.0 34.5 2.0 35.0 94-95 24.72168685174384 31.5 17.5 34.0 2.0 35.0 96-97 23.84059917617352 31.0 4.5 34.0 2.0 35.0 98-99 22.687952171692103 31.0 2.0 34.0 2.0 35.0 100-101 19.4824619876897 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20563812387775826 1101 2 0.13743579532895112 1101 3 0.13455878370665886 1101 4 0.14558930964923178 1101 5 0.12935795864089528 1101 6 0.1113424818645612 1101 7 0.08362581784857781 1101 8 0.17055955150022584 1101 9 0.31048081916751613 1101 10-11 0.41686306261403416 1101 12-13 0.31288031887415 1101 14-15 0.4656884416462006 1101 16-17 0.4928076582408991 1101 18-19 0.5566896768875935 1101 20-21 0.33425676483911104 1101 22-23 0.23388599126426612 1101 24-25 0.3862242703176193 1101 26-27 0.26966614072270545 1101 28-29 0.19419952676879149 1101 30-31 0.45731498251530667 1101 32-33 0.3796077555718895 1101 34-35 0.5811627561622785 1101 36-37 0.5440848626129338 1101 38-39 0.7528889286696696 1101 40-41 1.0023049631676884 1101 42-43 0.7298055124301399 1101 44-45 0.7134718564272404 1101 46-47 0.9117550118616222 1101 48-49 1.3281562926693766 1101 50-51 1.0256814055614996 1101 52-53 0.947778462066637 1101 54-55 1.1278650185676113 1101 56-57 1.3599433043850127 1101 58-59 1.5369036023154337 1101 60-61 1.5442720325610466 1101 62-63 1.4136440308452407 1101 64-65 1.4213574729996736 1101 66-67 1.5394611976314039 1101 68-69 1.6869205027050143 1101 70-71 1.6108909143195191 1101 72-73 1.6745516425781375 1101 74-75 1.5728331235283015 1101 76-77 1.6357926341937414 1101 78-79 1.8875223956705867 1101 80-81 1.9070536871951234 1101 82-83 2.208212239908235 1101 84-85 2.303617311777348 1101 86-87 2.5171259576753826 1101 88-89 2.2786992731409335 1101 90-91 2.300740865516225 1101 92-93 2.336408459312043 1101 94-95 2.7756206323630046 1101 96-97 2.75540806563383 1101 98-99 2.835117397988796 1101 100-101 3.659321895312498 1102 1 0.3368629621376975 1102 2 0.3064130342342857 1102 3 0.09173487280859405 1102 4 0.25095140373259994 1102 5 0.2895432743668849 1102 6 0.08474348755092365 1102 7 0.16442927039177846 1102 8 0.15416232105393135 1102 9 0.2835724431473352 1102 10-11 0.4543413275700985 1102 12-13 0.3383847285425219 1102 14-15 0.5567641500911407 1102 16-17 0.44615172202835396 1102 18-19 0.34705419902992674 1102 20-21 0.5700526199074929 1102 22-23 0.4466701133251405 1102 24-25 0.4945901083112645 1102 26-27 0.529174732918456 1102 28-29 0.5967188337537692 1102 30-31 0.5405132061160458 1102 32-33 0.5655030827332581 1102 34-35 0.6613650683010022 1102 36-37 0.6734142483923407 1102 38-39 0.7206388229316261 1102 40-41 0.7975021058905583 1102 42-43 1.0358936744678218 1102 44-45 1.113905774061216 1102 46-47 1.1247780235946223 1102 48-49 1.0352736384093078 1102 50-51 0.9747920310608222 1102 52-53 1.0694849175704988 1102 54-55 1.1819735293058997 1102 56-57 1.1667618422235648 1102 58-59 1.164909304337776 1102 60-61 1.1446957680122765 1102 62-63 1.3026797781391295 1102 64-65 1.4323072356764897 1102 66-67 1.4180699981702745 1102 68-69 1.2613522585832655 1102 70-71 1.3554414884379753 1102 72-73 1.1554992121694383 1102 74-75 1.0723670928564353 1102 76-77 0.8106716228680213 1102 78-79 1.318367751812012 1102 80-81 1.3865835836501574 1102 82-83 1.3843659595379165 1102 84-85 1.6686681443666025 1102 86-87 1.735749169919064 1102 88-89 1.643376106325448 1102 90-91 1.5847239004332785 1102 92-93 1.9926697184564226 1102 94-95 2.3389655577197708 1102 96-97 2.3601905585454652 1102 98-99 2.6409725446905483 1102 100-101 3.135074593590925 1103 1 0.09664145069623231 1103 2 -0.062464361567357685 1103 3 0.1111746807333418 1103 4 0.24379791620186353 1103 5 -0.05699591770014223 1103 6 0.027873261681605754 1103 7 0.1597610030980121 1103 8 0.07543490181845414 1103 9 0.19421757260901984 1103 10-11 0.10990912536485098 1103 12-13 0.17029935536892538 1103 14-15 0.39445192913882465 1103 16-17 0.4007794940047731 1103 18-19 0.3902176849983974 1103 20-21 0.3668956296345911 1103 22-23 0.36432483832699347 1103 24-25 0.1889059635641459 1103 26-27 0.1222481836128324 1103 28-29 0.0051654991596663535 1103 30-31 0.05548311213401291 1103 32-33 0.25111758375694393 1103 34-35 0.15264213027322882 1103 36-37 0.10687036955761897 1103 38-39 0.35783983593123736 1103 40-41 0.29299006418654727 1103 42-43 0.16061308508623284 1103 44-45 0.34849905307189744 1103 46-47 0.14064725455360616 1103 48-49 0.24628302016709114 1103 50-51 0.09940146708643738 1103 52-53 0.10731926088856625 1103 54-55 0.04846951266376465 1103 56-57 0.28610277579480226 1103 58-59 0.2948910506109641 1103 60-61 0.25785242938919595 1103 62-63 0.5614869957613777 1103 64-65 0.37921520235932604 1103 66-67 0.490753953802205 1103 68-69 0.5850044017516822 1103 70-71 0.6313471857385622 1103 72-73 0.8862855202298547 1103 74-75 0.8442386754216571 1103 76-77 0.9518625552058957 1103 78-79 0.9556643502053532 1103 80-81 1.1613741180238968 1103 82-83 1.0299836261853983 1103 84-85 1.2499198402364904 1103 86-87 1.4347168476667882 1103 88-89 1.423744510933279 1103 90-91 1.474207657083145 1103 92-93 1.2091942746524147 1103 94-95 1.6202682324663122 1103 96-97 1.6861963635594854 1103 98-99 2.5264499372032354 1103 100-101 3.189773078202709 1104 1 0.19412050870160513 1104 2 0.10712628170749738 1104 3 0.16811319819817783 1104 4 0.09388484523616114 1104 5 0.15064593467855047 1104 6 0.1619415404867297 1104 7 0.1439952373322484 1104 8 0.25952688854201966 1104 9 0.27114976796226387 1104 10-11 0.3570641751514785 1104 12-13 0.3203507225255606 1104 14-15 0.4649039601171694 1104 16-17 0.4979424464308764 1104 18-19 0.6967018006930346 1104 20-21 0.5451396631417822 1104 22-23 0.3917272407293879 1104 24-25 0.43035135632532473 1104 26-27 0.42385241942759677 1104 28-29 0.39927013010640877 1104 30-31 0.2758679706767708 1104 32-33 0.29219155246249073 1104 34-35 0.5234142181505845 1104 36-37 0.43700313390091594 1104 38-39 0.7579702294826873 1104 40-41 0.818950234883566 1104 42-43 0.3924962840746886 1104 44-45 0.4125697026688613 1104 46-47 0.6896041061420348 1104 48-49 0.5723261685786625 1104 50-51 0.5016655732979132 1104 52-53 0.7841605758877748 1104 54-55 0.7895395300495736 1104 56-57 1.0798833985227887 1104 58-59 1.1231074248799757 1104 60-61 1.1998668121930578 1104 62-63 1.1053071316867715 1104 64-65 1.2073723068322195 1104 66-67 1.5082780568526246 1104 68-69 1.4838111031899643 1104 70-71 1.3244481814711406 1104 72-73 1.3355308181593628 1104 74-75 1.5075072071112423 1104 76-77 1.3652891396851103 1104 78-79 1.3719753980953513 1104 80-81 1.634268064602054 1104 82-83 1.4229818876047133 1104 84-85 1.6161083972145214 1104 86-87 1.6288254299332614 1104 88-89 1.8021624025090652 1104 90-91 1.931553160481279 1104 92-93 1.7503472697527762 1104 94-95 1.6117531384249055 1104 96-97 1.698247888768904 1104 98-99 2.2516949190271625 1104 100-101 2.8992127810634614 1105 1 0.3210597120559022 1105 2 0.289769997916256 1105 3 0.2557276746408519 1105 4 0.260330834422966 1105 5 0.18319034602137663 1105 6 0.35553637663370097 1105 7 0.3813504177360869 1105 8 0.2841710461057403 1105 9 0.3721262658106639 1105 10-11 0.37200955747521647 1105 12-13 0.333594129785844 1105 14-15 0.3457160532430663 1105 16-17 0.28667686108386903 1105 18-19 0.2948729360786757 1105 20-21 0.429355706394567 1105 22-23 0.11810647497853921 1105 24-25 0.05610717669384968 1105 26-27 0.15545895148100186 1105 28-29 0.47032827862235393 1105 30-31 0.5407940437210144 1105 32-33 0.40745967560052776 1105 34-35 0.5226721901245455 1105 36-37 0.7030739713719498 1105 38-39 0.6842280911179017 1105 40-41 0.7297248128378797 1105 42-43 0.7763233832692222 1105 44-45 0.774130444145193 1105 46-47 0.9307328714515322 1105 48-49 1.1543106108163315 1105 50-51 1.0096513396004667 1105 52-53 0.8420746220093704 1105 54-55 0.8669200883635924 1105 56-57 1.1432178352435258 1105 58-59 1.0009982998661755 1105 60-61 1.157052622631106 1105 62-63 1.473088219626753 1105 64-65 1.3543379914841083 1105 66-67 1.568724377055652 1105 68-69 1.5590455730123196 1105 70-71 1.3485296114612026 1105 72-73 1.3267119943703278 1105 74-75 1.4400571353910614 1105 76-77 1.4328550670822189 1105 78-79 1.6247724851526115 1105 80-81 1.8458812169798549 1105 82-83 1.7187890154873102 1105 84-85 1.668422752284588 1105 86-87 1.710511957573118 1105 88-89 1.6956855184750843 1105 90-91 1.755350468216026 1105 92-93 1.8760975731843104 1105 94-95 1.6219953319590523 1105 96-97 1.7841190131206623 1105 98-99 1.8114736898535995 1105 100-101 2.2036180552552302 1106 1 -0.019177631067123002 1106 2 -0.07651532608271694 1106 3 -0.042684346190700495 1106 4 0.07493853526818128 1106 5 -0.04174775694114885 1106 6 0.19188914705971882 1106 7 0.14909565806734548 1106 8 0.2755822985602734 1106 9 0.3819102664936622 1106 10-11 -0.06386938045699964 1106 12-13 -0.12027336786633214 1106 14-15 -9.543130166349556E-4 1106 16-17 -0.05350498814348725 1106 18-19 0.14703719800986192 1106 20-21 0.19784797993678183 1106 22-23 0.2531658107592918 1106 24-25 -0.05909388195892262 1106 26-27 0.05516013819661936 1106 28-29 0.21529114039986297 1106 30-31 0.33001115291592953 1106 32-33 0.31966833264825567 1106 34-35 0.38433017697675353 1106 36-37 0.16201702609746604 1106 38-39 -0.22374168605276168 1106 40-41 0.12205811771280395 1106 42-43 0.5709746254787333 1106 44-45 0.30183897313008146 1106 46-47 0.44131086171876177 1106 48-49 0.5236737116444701 1106 50-51 0.3111539435448023 1106 52-53 0.5043088916243263 1106 54-55 0.20425467386687757 1106 56-57 0.14718778316463954 1106 58-59 -0.04751190247309722 1106 60-61 0.3577185493791575 1106 62-63 0.6144098280959653 1106 64-65 0.6274789459791137 1106 66-67 0.7745683111459769 1106 68-69 0.17098529810409246 1106 70-71 0.2596113155506785 1106 72-73 0.46387156066419166 1106 74-75 0.7541995412648781 1106 76-77 1.090534568490913 1106 78-79 0.7385904651748127 1106 80-81 1.0449677020818768 1106 82-83 1.0286882727228601 1106 84-85 0.8505921367292224 1106 86-87 1.2846859522798546 1106 88-89 1.4667518550242171 1106 90-91 1.521319903885793 1106 92-93 1.6396893088626179 1106 94-95 2.016475823720345 1106 96-97 2.303008281454993 1106 98-99 2.2392547877789823 1106 100-101 2.3504677094855744 1107 1 -0.12889536431426762 1107 2 -0.056950577106203326 1107 3 -0.11956432895303593 1107 4 -0.16509594006041084 1107 5 -0.03235322989956302 1107 6 0.04264329487268981 1107 7 0.19963433507660966 1107 8 0.14549180082271818 1107 9 -0.0019078761480102457 1107 10-11 0.0405854784096249 1107 12-13 -0.023279185578033434 1107 14-15 0.17390563096095235 1107 16-17 0.17578288085026372 1107 18-19 0.30516044730957503 1107 20-21 0.06677709004236476 1107 22-23 -0.16107142259558316 1107 24-25 0.12450340310895314 1107 26-27 0.2639944411485615 1107 28-29 0.23394013846062478 1107 30-31 -0.031128687635685992 1107 32-33 -0.06100393625931133 1107 34-35 0.2716389466384683 1107 36-37 0.1115645374096843 1107 38-39 0.23058927710172838 1107 40-41 0.38035374365549757 1107 42-43 0.6100610292710158 1107 44-45 0.5215922590598367 1107 46-47 0.6035765372197304 1107 48-49 0.5107346898819216 1107 50-51 0.3794851221700952 1107 52-53 0.3206685123957129 1107 54-55 0.7273423704839885 1107 56-57 0.6347497310207046 1107 58-59 0.7657557122651042 1107 60-61 1.0317381572222928 1107 62-63 0.8548267641011407 1107 64-65 0.5868626994805126 1107 66-67 0.9082571713054293 1107 68-69 1.3089781875675754 1107 70-71 1.136916853150339 1107 72-73 0.9383921381259448 1107 74-75 1.2559825621655456 1107 76-77 1.3075406016734163 1107 78-79 1.3307473279199087 1107 80-81 1.244084271786683 1107 82-83 1.098775538398364 1107 84-85 1.3512170020599648 1107 86-87 1.575943224419479 1107 88-89 1.2677430207212659 1107 90-91 1.3308221663292308 1107 92-93 1.9630247969039907 1107 94-95 1.918603597906941 1107 96-97 1.8300983482509459 1107 98-99 2.1527809674816325 1107 100-101 2.8798309932606223 1108 1 0.22454378912488338 1108 2 0.12717640702078015 1108 3 -0.02855903794774406 1108 4 -0.07779435275882207 1108 5 -0.05827915582548826 1108 6 -0.1301080278786273 1108 7 0.19460788005015672 1108 8 0.07009497542589571 1108 9 0.09967942543929098 1108 10-11 -0.06907060624646277 1108 12-13 -0.05290881520766533 1108 14-15 0.16742414947947282 1108 16-17 0.1248569549243328 1108 18-19 0.1945784367275678 1108 20-21 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-2.3942467758682646 2316 86-87 -2.7226857532379363 2316 88-89 -2.555581285304463 2316 90-91 -2.59129473730426 2316 92-93 -2.976853334443337 2316 94-95 -2.7467146146861516 2316 96-97 -2.9988849512299858 2316 98-99 -3.8101901451340296 2316 100-101 -3.9280058381544087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2228.0 3 431.0 4 435.0 5 455.0 6 515.0 7 600.0 8 715.0 9 922.0 10 1145.0 11 1542.0 12 2167.0 13 2923.0 14 3159.0 15 4012.0 16 5374.0 17 7468.0 18 6518.0 19 4163.0 20 4051.0 21 4148.0 22 4387.0 23 4988.0 24 5530.0 25 6305.0 26 7234.0 27 8360.0 28 9865.0 29 11573.0 30 14225.0 31 17771.0 32 22448.0 33 29120.0 34 39358.0 35 56367.0 36 77674.0 37 104126.0 38 73456.0 39 6299.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.732038584554914 19.59831669339591 14.606698717541224 21.06294600450795 2 14.731443406588419 21.06061018672331 42.25411435781524 21.953832048873036 3 19.22913519637462 27.06451167789914 30.27030560074367 23.43604752498257 4 11.733457274042712 17.375785447012625 38.525166076314314 32.36559120263035 5 12.29266521423384 38.77614379084967 34.42774146695715 14.503449527959331 6 28.91956092134656 37.61185200240263 18.75520584760726 14.713381228643549 7 26.9243749931956 34.53858911223449 21.348759058924287 17.18827683564562 8 25.969489501695737 35.505698716541254 20.096390964072956 18.428420817690064 9 26.86339658523219 17.469790972949333 21.13661067328159 34.530201768536884 10-11 24.05090511183355 28.20840391641995 27.38463464803122 20.356056323715283 12-13 25.607873845704347 25.67456597938986 28.429994582965467 20.287565591940325 14-15 22.383949610254337 26.688981053941962 27.776512549801343 23.150556786002365 16-17 20.320442740819704 30.39352219374447 28.20531198258069 21.080723082855137 18-19 20.190586610855128 28.279268172090422 31.88170711461052 19.648438102443933 20-21 21.159777914867366 27.263671662372534 31.833109554741085 19.743440868019015 22-23 20.70648648403442 27.276529533711663 31.79878698801958 20.218196994234336 24-25 20.48367444514706 27.42470031182268 32.150669234796034 19.940956008234224 26-27 20.407337445176932 27.527849302565276 32.10477035553112 19.960042896726673 28-29 19.97691757013874 27.92701814747168 32.27248518106188 19.82357910132769 30-31 20.27011455143473 27.684337076102985 32.183100827946014 19.862447544516275 32-33 20.086357619258894 27.897013231279278 31.92101698390707 20.095612165554762 34-35 20.46086787679946 27.81784132295593 31.778688331225307 19.942602469019306 36-37 20.404458295341655 27.666471604904668 31.918908742849627 20.010161356904053 38-39 20.268768478626708 28.031991668231594 31.938506033365176 19.76073381977652 40-41 20.384416159340027 28.030919483610877 31.78409339345959 19.800570963589507 42-43 20.250938979913997 28.13728158510696 31.40946782065937 20.202311614319672 44-45 20.270264140330504 28.293815859306605 31.13526361867263 20.300656381690263 46-47 20.489159575655247 28.53140534032772 30.694500266281505 20.28493481773553 48-49 20.453747431168935 28.687888727084665 30.501154556301064 20.357209285445332 50-51 20.56162271967222 28.994219737089217 30.22167503327439 20.22248250996417 52-53 20.585486595906456 29.178333511776334 29.964722726802563 20.27145716551465 54-55 20.29127517265657 29.072535719859044 30.05059028042403 20.585598827060355 56-57 20.593574044357037 29.30632372164413 29.775039845859798 20.32506238813904 58-59 20.718971203419162 29.04782982552254 29.909604786247883 20.32359418481041 60-61 20.606837467274104 29.029480975889427 30.28680858321132 20.07687297362515 62-63 20.564623665862964 29.08613767441523 30.212228251502633 20.137010408219172 64-65 20.462270749250912 29.111409264465347 30.20852893977454 20.217791046509202 66-67 20.443323611722178 29.175537825836507 29.868690213225324 20.512448349215997 68-69 20.222451227101278 29.20522808462617 30.06375447451545 20.508566213757103 70-71 20.420184228622023 29.342136536741396 29.82774605697407 20.409933177662506 72-73 20.459269164716908 29.164644330397277 29.721765842037318 20.654320662848498 74-75 20.280643373163873 29.572158116149378 29.534235787972452 20.612962722714297 76-77 20.2596862970661 29.375560783232164 29.725117554681184 20.639635365020553 78-79 20.34245351230766 29.171327456428987 29.608148920284393 20.87807011097896 80-81 20.31512664124679 29.56426048751369 29.519263132015528 20.601349739223995 82-83 20.483824000333833 29.634061267681655 29.458798214348892 20.423316517635616 84-85 20.32427989959914 29.434106719908996 29.394374540199802 20.84723884029206 86-87 20.28444247696094 29.409171355818884 29.533721528311354 20.77266463890882 88-89 20.502715702630788 29.083102224181772 29.432192711012405 20.981989362175028 90-91 20.278389355018092 29.013435951307265 29.755220934356974 20.95295375931767 92-93 20.564801804114595 29.07610790942053 29.486133953331418 20.87295633313346 94-95 20.25772213906654 29.09157708531773 29.49072436159115 21.159976414024584 96-97 20.117863292370274 28.970855325348772 29.572872573015914 21.33840880926504 98-99 20.244488796153497 29.22180894493332 29.148507665789715 21.38519459312347 100-101 20.32696801253955 28.852936054105832 29.73616513308757 21.083930800267044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1028.0 1 675.0 2 737.5 3 1012.5 4 660.0 5 492.5 6 738.0 7 1460.0 8 2286.5 9 2523.0 10 2059.0 11 1557.0 12 1458.5 13 1505.5 14 1580.0 15 1624.5 16 1605.0 17 1652.0 18 1676.5 19 1722.0 20 1876.5 21 2093.5 22 2420.0 23 2769.0 24 3230.5 25 3835.0 26 4658.0 27 5640.0 28 6394.0 29 7272.0 30 8425.5 31 9484.5 32 10540.0 33 11721.5 34 13290.5 35 14632.0 36 15948.5 37 17241.0 38 17768.5 39 18111.5 40 18449.0 41 19050.5 42 19361.5 43 19736.0 44 20532.5 45 20927.0 46 20559.5 47 20720.5 48 22763.5 49 22149.5 50 19071.0 51 16888.5 52 15122.5 53 13992.5 54 12810.5 55 11322.0 56 9919.5 57 8591.0 58 7261.5 59 6308.5 60 5368.5 61 4235.5 62 3284.5 63 2376.5 64 1699.5 65 1294.0 66 911.5 67 656.0 68 519.0 69 371.5 70 233.5 71 164.0 72 137.5 73 109.0 74 76.0 75 58.5 76 46.5 77 29.0 78 13.5 79 12.0 80 10.5 81 5.5 82 4.5 83 3.0 84 2.5 85 1.5 86 0.5 87 0.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2671820714490144 2 0.22515750156686434 3 0.23077285357697924 4 0.22769346699078719 5 0.22787460737821028 6 0.18023468548594532 7 0.17099652572736923 8 0.17552503541294573 9 0.17769872006202247 10-11 0.17996297490481072 12-13 0.18449148459038722 14-15 0.17996297490481072 16-17 0.17126823630850382 18-19 0.1661963054606581 20-21 0.16628687565436964 22-23 0.1721739382456191 24-25 0.1709059555336577 26-27 0.1751627546380996 28-29 0.17923841335511848 30-31 0.18032525567965685 32-33 0.17706472870604176 34-35 0.17914784316140694 36-37 0.1721739382456191 38-39 0.16565288429838892 40-41 0.16021867267569712 42-43 0.16809827952860026 44-45 0.1685511304971579 46-47 0.17199279785819607 48-49 0.17760814986831094 50-51 0.1736230613450036 52-53 0.18023468548594532 54-55 0.17461933347583045 56-57 0.15786384763919734 58-59 0.14708599458752522 60-61 0.1610338044191009 62-63 0.15559959279640906 64-65 0.1607620938379663 66-67 0.15949411112600487 68-69 0.1595846813197164 70-71 0.16212064674363927 72-73 0.16692086701035036 74-75 0.16873227088458098 76-77 0.16873227088458098 78-79 0.1661963054606581 80-81 0.16565288429838892 82-83 0.16058095345054324 84-85 0.1571392860895051 86-87 0.15840726880146652 88-89 0.16510946313611977 90-91 0.1607620938379663 92-93 0.15822612841404343 94-95 0.15994696209456252 96-97 0.16501889294240824 98-99 0.16447547178013905 100-101 0.15252020621021706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 552058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.61054479680848 #Duplication Level Percentage of deduplicated Percentage of total 1 86.59479364080899 60.27910761903921 2 8.362001760122332 11.641669962279739 3 2.114919100795293 4.4166201242261 4 0.8852670556425801 2.4649568813578626 5 0.4835203723228566 1.6829058268874861 6 0.3007071786026122 1.2559434316103413 7 0.22035817653725973 1.0737476903432968 8 0.16288827494245442 0.9070993247805264 9 0.11566202340828328 0.7246166815576635 >10 0.7074951637588329 8.8321474494388 >50 0.035967371764405995 1.7161286601927894 >100 0.013813550929940964 1.8315164329559912 >500 0.0013031651820693485 0.7151894778316825 >1k 0.0013031651820693485 2.4583504374985536 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4740 0.8586054363853074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3738 0.6771027681874006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.48273913248245653 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.2543211039419771 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1003 0.18168380858532981 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 978 0.1771552988997533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 813 0.14726713497494828 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 764 0.13839125599121832 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.13168906165656508 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 660 0.11955265569922001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.811403874230606E-4 0.0 3 0.0 0.0 0.0 1.811403874230606E-4 0.0 4 0.0 0.0 0.0 1.811403874230606E-4 0.0 5 0.0 0.0 0.0 1.811403874230606E-4 0.0 6 0.0 0.0 0.0 1.811403874230606E-4 0.0 7 0.0 0.0 0.0 1.811403874230606E-4 0.0 8 0.0 0.0 0.0 1.811403874230606E-4 0.0 9 0.0 0.0 0.0 1.811403874230606E-4 0.0 10-11 0.0 0.0 0.0 1.811403874230606E-4 0.0 12-13 0.0 0.0 0.0 1.811403874230606E-4 0.0 14-15 0.0 0.0 0.0 1.811403874230606E-4 0.0 16-17 0.0 0.0 0.0 1.811403874230606E-4 0.0 18-19 9.05701937115303E-5 0.0 0.0 2.717105811345909E-4 0.0 20-21 1.811403874230606E-4 0.0 0.0 5.434211622691819E-4 0.0 22-23 1.811403874230606E-4 0.0 0.0 5.434211622691819E-4 0.0 24-25 1.811403874230606E-4 0.0 0.0 0.0013585529056729545 0.0 26-27 1.811403874230606E-4 0.0 0.0 0.001992544261653667 0.0 28-29 1.811403874230606E-4 0.0 0.0 0.004166228910730394 0.0 30-31 1.811403874230606E-4 0.0 0.0 0.006339913559807122 0.0 32-33 1.811403874230606E-4 0.0 0.0 0.009147589564864562 0.0 34-35 1.811403874230606E-4 0.0 0.0 0.011411844407652819 0.0 36-37 1.811403874230606E-4 0.0 0.0 0.016664915642921576 0.0 38-39 1.811403874230606E-4 0.0 0.0 0.022823688815305637 0.0 40-41 1.811403874230606E-4 0.0 0.0 0.027080487919747563 0.0 42-43 1.811403874230606E-4 0.0 0.0 0.039941455426784865 0.0 44-45 1.811403874230606E-4 0.0 0.0 0.053798695064649 0.0 46-47 1.811403874230606E-4 0.0 0.0 0.06412369714776346 0.0 48-49 1.811403874230606E-4 0.0 0.0 0.07653181368624311 9.05701937115303E-5 50-51 1.811403874230606E-4 0.0 0.0 0.0947364226222607 1.811403874230606E-4 52-53 1.811403874230606E-4 0.0 0.0 0.12770397313325774 1.811403874230606E-4 54-55 1.811403874230606E-4 0.0 0.0 0.14618029265040994 1.811403874230606E-4 56-57 1.811403874230606E-4 0.0 0.0 0.18929170485709834 1.811403874230606E-4 58-59 1.811403874230606E-4 0.0 0.0 0.2543211039419771 1.811403874230606E-4 60-61 1.811403874230606E-4 0.0 0.0 0.31663339721551 1.811403874230606E-4 62-63 1.811403874230606E-4 0.0 0.0 0.4264044719938847 1.811403874230606E-4 64-65 1.811403874230606E-4 0.0 0.0 0.49378869611526327 1.811403874230606E-4 66-67 1.811403874230606E-4 0.0 0.0 0.6239380644787322 1.811403874230606E-4 68-69 1.811403874230606E-4 0.0 0.0 0.7858775708349486 1.811403874230606E-4 70-71 1.811403874230606E-4 0.0 0.0 0.903981103434784 1.811403874230606E-4 72-73 1.811403874230606E-4 0.0 0.0 1.0842157889207293 1.811403874230606E-4 74-75 1.811403874230606E-4 0.0 0.0 1.2837419256672307 1.811403874230606E-4 76-77 1.811403874230606E-4 0.0 0.0 1.4138912940306998 1.811403874230606E-4 78-79 1.811403874230606E-4 0.0 0.0 1.553641102927591 1.811403874230606E-4 80-81 1.811403874230606E-4 0.0 0.0 1.7831459737926088 1.811403874230606E-4 82-83 1.811403874230606E-4 0.0 0.0 1.9776001796912643 1.811403874230606E-4 84-85 1.811403874230606E-4 0.0 0.0 2.2502164627629706 1.811403874230606E-4 86-87 1.811403874230606E-4 0.0 0.0 2.636770049523782 1.811403874230606E-4 88-89 1.811403874230606E-4 0.0 0.0 3.1389817736542174 1.811403874230606E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 25 5.2533105E-5 76.057076 5 TACCTGG 580 0.0 36.061543 2 GTCCGGG 55 0.0025644174 34.583954 1 GTACCTG 635 0.0 34.4478 1 GTACACG 75 3.0563492E-4 31.701956 1 AGGCCGT 60 0.003945199 31.673199 6 GTCCATA 135 4.177473E-7 28.17952 1 GTATCAA 5550 0.0 27.931995 1 TATAACG 70 0.008357184 27.163244 2 GTACATG 3355 0.0 26.788391 1 TACATGG 3405 0.0 26.245834 2 GAGTACT 3220 0.0 25.739763 12-13 GTCATAC 95 0.0012178102 25.027863 1 GGTATCA 3950 0.0 24.920147 1 CAACGCA 6275 0.0 24.847332 5 TCAACGC 6260 0.0 24.754997 4 ATCAACG 6270 0.0 24.715517 3 ACCTGGG 830 0.0 24.626915 3 TATCAAC 6275 0.0 24.620071 2 AACGCAG 6400 0.0 24.348772 6 >>END_MODULE