FastQCFastQC Report
Thu 26 May 2016
SRR937640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552058
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59511.0779664455546338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49870.9033471120788034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38810.7030058435888983No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.33637769944462353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.21773074568251888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11930.21610048219571132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.1867557394331755No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.17588731618779185No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8720.15795441783290887No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6000.10868423245383638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA802.260931E-741.5597276
TAACGCA608.2839884E-539.584284
GTACCTG5750.036.3651661
TACCTGG5650.035.3105742
ATAACGC702.0516675E-433.929383
GTACACG753.0677975E-431.681771
CATTCCG753.075961E-431.6674219
GGACGGA600.0039504831.6645536
GGTATCA37850.029.6310351
ACCTGGG6450.027.2487133
GCGACTA1056.898322E-527.1435059
ATACGTC700.00838688427.1435035
GTATCAA53650.026.8394491
TATAACG908.940391E-426.3895172
CTTACAC2207.6397555E-1125.9097083
GTACATG28350.025.4795171
AGGACCG1351.3408362E-524.6302195
TACATGG28950.024.6119862
AGCGACT1201.7089714E-423.7505668
ACATGGG28050.023.3695413
Produced by FastQC (version 0.11.5)