FastQCFastQC Report
Thu 26 May 2016
SRR937639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442970
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117830.4823227464929983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88640.36283703852278165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60430.2473628411319009No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41350.16926118617911803No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31480.1288595439158074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153150.029.5929871
GGTATCA105800.028.6329921
GTACATG123700.027.4883881
GGACCGA3400.026.544266
TACATGG125550.026.2419262
ACATGGG127750.025.0462233
ATCAACG182450.024.6510473
TATCAAC183700.024.6122062
TCAACGC183450.024.542594
CAACGCA185450.024.3035455
AACGCAG189800.023.800186
TATGACG2554.8567017E-1022.3732574
GAGTACT96150.022.18206212-13
CATGGGG53800.021.4739574
AGTACTT100700.021.13262412-13
ACGCAGA213850.020.7916647
CGCAGAG213450.020.6303338
GTACTTT104150.020.40895714-15
CCGTGCG1404.8516932E-420.3585079
AGAGTAC165500.020.04851310-11
Produced by FastQC (version 0.11.5)