FastQCFastQC Report
Thu 26 May 2016
SRR937639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442970
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145920.5973057385068176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119110.4875622705149879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80190.3282479932213658No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45380.185757500092101No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30250.12382468880092674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119000.029.3540211
GTATCAA170200.029.2636811
GTACATG119800.025.2305971
ATCAACG205600.024.1975153
TATCAAC206500.024.1380752
TACATGG122600.024.107222
TCAACGC207000.024.0109044
CAACGCA209650.023.7527335
AACGCAG214750.023.1791486
ACATGGG122900.023.1591243
GAGTACT119050.022.60441412-13
GGACCGA4250.022.3516946
TACCTGG21050.022.3475692
GTACCTG21250.021.6940211
AGTACTT124700.021.61832812-13
GTACTTT126950.021.0829114-15
ACGCAGA239050.020.7460047
ACCGGTC1150.003723015520.6535578
AGAGTAC189150.020.60605810-11
CGCAGAG238950.020.5956468
Produced by FastQC (version 0.11.5)