##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937639_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2442970 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11497889863568 33.0 31.0 34.0 31.0 34.0 2 32.39237853923708 34.0 31.0 34.0 31.0 34.0 3 32.48702972201869 34.0 31.0 34.0 31.0 34.0 4 36.13063156731356 37.0 35.0 37.0 35.0 37.0 5 36.04526621284748 37.0 35.0 37.0 35.0 37.0 6 36.08991882831144 37.0 35.0 37.0 35.0 37.0 7 36.03724851308039 37.0 35.0 37.0 35.0 37.0 8 36.01295554182 37.0 35.0 37.0 35.0 37.0 9 37.70571312787304 39.0 37.0 39.0 35.0 39.0 10-11 37.61495004031977 39.0 37.0 39.0 35.0 39.0 12-13 37.50751810296484 39.0 37.0 39.0 35.0 39.0 14-15 38.70845118851234 40.0 38.0 41.0 34.5 41.0 16-17 38.668519466059756 40.0 38.0 41.0 34.5 41.0 18-19 38.65836133886212 40.0 38.0 41.0 34.0 41.0 20-21 38.56661747790599 40.0 38.0 41.0 34.0 41.0 22-23 38.511501983241715 40.0 38.0 41.0 34.0 41.0 24-25 38.53865029042518 40.0 38.0 41.0 34.0 41.0 26-27 38.40678763963536 40.0 38.0 41.0 34.0 41.0 28-29 38.261091826751866 40.0 38.0 41.0 34.0 41.0 30-31 38.29920138192446 40.0 38.0 41.0 34.0 41.0 32-33 38.21377626413751 40.0 38.0 41.0 34.0 41.0 34-35 38.062541905958724 40.0 37.0 41.0 33.5 41.0 36-37 37.90895815339525 40.0 37.0 41.0 33.0 41.0 38-39 37.7797056451778 40.0 37.0 41.0 33.0 41.0 40-41 37.59774311596131 40.0 37.0 41.0 33.0 41.0 42-43 37.37138012337442 40.0 37.0 41.0 33.0 41.0 44-45 37.05467463783837 40.0 36.0 41.0 31.5 41.0 46-47 36.652773673029145 39.5 36.0 41.0 31.0 41.0 48-49 36.297692562741254 39.0 36.0 41.0 30.0 41.0 50-51 36.030964563625425 39.0 35.5 40.5 29.5 41.0 52-53 35.79183391527526 39.0 35.0 40.0 28.5 41.0 54-55 35.566183989160734 39.0 35.0 40.0 28.0 41.0 56-57 35.289710475364004 38.0 35.0 40.0 28.0 41.0 58-59 35.020842253486535 38.0 34.0 40.0 27.0 41.0 60-61 34.68617256863571 38.0 34.0 40.0 26.0 41.0 62-63 34.31167063042116 37.5 34.0 40.0 26.0 41.0 64-65 33.89391048600679 37.0 33.0 39.5 25.5 41.0 66-67 33.456701269356564 36.0 33.0 39.0 24.5 40.0 68-69 32.99778445908055 36.0 32.0 39.0 23.0 40.0 70-71 32.588218848368996 35.0 32.0 38.0 22.0 40.0 72-73 32.08669079030852 35.0 31.0 37.5 21.5 39.5 74-75 31.600396648341977 35.0 31.0 37.0 20.0 39.0 76-77 30.382647965386397 33.5 29.5 35.5 19.5 38.0 78-79 30.977805499044194 34.0 31.0 36.0 19.5 38.0 80-81 30.775765359378134 34.0 31.0 36.0 18.0 37.0 82-83 30.394380610486415 34.0 30.5 35.0 16.5 37.0 84-85 29.998426710111055 34.0 30.0 35.0 11.5 36.5 86-87 29.610134385604407 34.0 30.0 35.0 7.0 36.0 88-89 29.146795498921396 34.0 29.0 35.0 2.0 36.0 90-91 28.57687630220592 33.5 29.0 35.0 2.0 35.0 92-93 28.09853150059149 33.0 28.0 35.0 2.0 35.0 94-95 27.59454884832806 33.0 27.0 35.0 2.0 35.0 96-97 26.982032116644902 33.0 26.0 34.5 2.0 35.0 98-99 26.0254937637384 32.0 24.5 34.0 2.0 35.0 100-101 23.141456301141645 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4247928771095104 1101 2 0.34700008282382555 1101 3 0.2857568721916408 1101 4 0.11242860313616632 1101 5 0.07955679275075056 1101 6 0.24101869359146377 1101 7 0.2485121894449307 1101 8 0.13803840557170588 1101 9 0.1744734126164431 1101 10-11 0.32703702622156783 1101 12-13 0.2937588890921319 1101 14-15 0.3767497132133215 1101 16-17 0.29030725085441134 1101 18-19 0.3254152558823833 1101 20-21 0.29353981337077073 1101 22-23 0.2321920297888056 1101 24-25 0.25752955026555213 1101 26-27 0.20744253470986962 1101 28-29 0.2372612397471201 1101 30-31 0.2802929064853572 1101 32-33 0.24086049273994803 1101 34-35 0.32792232283956224 1101 36-37 0.33987235216903855 1101 38-39 0.3630319154557 1101 40-41 0.5170937206045281 1101 42-43 0.4371953425691544 1101 44-45 0.4992683616405955 1101 46-47 0.4953942201994863 1101 48-49 0.4551223649541569 1101 50-51 0.4037017859903358 1101 52-53 0.32561891806600585 1101 54-55 0.2936297485798107 1101 56-57 0.21427039542844994 1101 58-59 0.3521449935748109 1101 60-61 0.3615271557625661 1101 62-63 0.28040386495045766 1101 64-65 0.33142511036511024 1101 66-67 0.30921549047998553 1101 68-69 0.32211563503926754 1101 70-71 0.27250645068110657 1101 72-73 0.029749741329133883 1101 74-75 0.06082767154695645 1101 76-77 0.16866484356697953 1101 78-79 0.17771729059222352 1101 80-81 0.21234506224190142 1101 82-83 0.10552953032564005 1101 84-85 0.06278782709992115 1101 86-87 0.07947063532703424 1101 88-89 0.21288626171545744 1101 90-91 0.16767598339507117 1101 92-93 -0.06143480140515223 1101 94-95 -0.13040681438030433 1101 96-97 0.08267818803164673 1101 98-99 0.09558988781580524 1101 100-101 0.07788755102832567 1102 1 0.40780214357486955 1102 2 0.29351649370283894 1102 3 0.2603873031393533 1102 4 0.11583550784618524 1102 5 0.16321336280958576 1102 6 0.19455541183073422 1102 7 0.1564508419161612 1102 8 0.224803236585835 1102 9 0.25617209165223187 1102 10-11 0.2594612497789299 1102 12-13 0.31121232328777637 1102 14-15 0.4137821614072479 1102 16-17 0.41080585517737944 1102 18-19 0.3586192945255178 1102 20-21 0.308296529742222 1102 22-23 0.3133030717213785 1102 24-25 0.2452315083641068 1102 26-27 0.3043108222297164 1102 28-29 0.31000818119094475 1102 30-31 0.31269398982128394 1102 32-33 0.2904921170174646 1102 34-35 0.2254285748500635 1102 36-37 0.17435771571955883 1102 38-39 0.2537371214866866 1102 40-41 0.3052525683667753 1102 42-43 0.2769762887240361 1102 44-45 0.3005117075298287 1102 46-47 0.21733281041002783 1102 48-49 0.26086601695842404 1102 50-51 0.29941565663338565 1102 52-53 0.4291141291816132 1102 54-55 0.2614029951632588 1102 56-57 0.2616617764742202 1102 58-59 0.29806930533482046 1102 60-61 0.15899335339873488 1102 62-63 0.07610227418993532 1102 64-65 -0.07986742146566428 1102 66-67 0.07812457950238638 1102 68-69 0.11601232380847648 1102 70-71 0.02483053830264481 1102 72-73 0.0631596553056184 1102 74-75 0.10376741358018293 1102 76-77 -0.07661942834618429 1102 78-79 0.10676200414118497 1102 80-81 0.11089698246520996 1102 82-83 0.20621408656260343 1102 84-85 0.16373792516050045 1102 86-87 -0.10551125719026899 1102 88-89 -0.17019055759365997 1102 90-91 0.13252546818789668 1102 92-93 0.2515399103797584 1102 94-95 0.006339545963786719 1102 96-97 0.04978244354950334 1102 98-99 0.24514119580903682 1102 100-101 0.3274258880454184 1103 1 0.43315638440446236 1103 2 0.3710116569902553 1103 3 0.30539155944642715 1103 4 0.19362648447518893 1103 5 0.15909635305877146 1103 6 0.20197957217742868 1103 7 0.19544383835633994 1103 8 0.2528536544777964 1103 9 0.32039028543028536 1103 10-11 0.31542046661843415 1103 12-13 0.34839329372579186 1103 14-15 0.37302202365767556 1103 16-17 0.34004649130181264 1103 18-19 0.3364104646230217 1103 20-21 0.3389360398799681 1103 22-23 0.2602650358136245 1103 24-25 0.1892765478316889 1103 26-27 0.2861136584779729 1103 28-29 0.3420228654503461 1103 30-31 0.37601294199587443 1103 32-33 0.3477220349868162 1103 34-35 0.37634010170349796 1103 36-37 0.45967461712800883 1103 38-39 0.4993818552740308 1103 40-41 0.494604352146375 1103 42-43 0.42727326287664624 1103 44-45 0.4121011480189196 1103 46-47 0.32366551819520595 1103 48-49 0.24529093183228667 1103 50-51 0.20410871885285786 1103 52-53 0.20403364628422338 1103 54-55 0.05494685447295922 1103 56-57 0.23816996019190384 1103 58-59 0.11692358485811383 1103 60-61 0.21226413191599391 1103 62-63 0.26189114201226005 1103 64-65 0.2609820019981939 1103 66-67 0.1778455011863329 1103 68-69 0.2985728794500133 1103 70-71 0.08413946880875756 1103 72-73 0.09216329251348299 1103 74-75 0.1387828910604476 1103 76-77 0.14514577827817376 1103 78-79 0.1334854215688317 1103 80-81 0.20515048424512017 1103 82-83 0.2835702427303808 1103 84-85 0.10935547662311862 1103 86-87 0.10837862053763558 1103 88-89 0.11539236300686895 1103 90-91 0.1805073982296861 1103 92-93 0.2792543702156962 1103 94-95 0.1836128976121394 1103 96-97 0.20230111326007005 1103 98-99 0.5271656502278574 1103 100-101 0.5461126238448308 1104 1 0.30028662165463516 1104 2 0.18188570967828355 1104 3 0.15542777801996266 1104 4 0.09219336958899049 1104 5 0.12620485175816754 1104 6 0.08874912724447626 1104 7 0.09287028280341758 1104 8 0.15335238201339507 1104 9 0.08085571261687363 1104 10-11 0.19225991094108252 1104 12-13 0.18788224150586075 1104 14-15 0.13617977221040434 1104 16-17 0.1474568188540033 1104 18-19 0.1297962790891134 1104 20-21 0.18425745564845641 1104 22-23 0.1709312575868367 1104 24-25 0.11730083575526606 1104 26-27 0.25895722757027073 1104 28-29 -0.0038623824061616574 1104 30-31 0.06605435263895032 1104 32-33 0.1418187037668588 1104 34-35 0.15954147919435258 1104 36-37 0.25800937747808206 1104 38-39 0.23078196978101317 1104 40-41 0.18091660143088717 1104 42-43 0.3208800921145851 1104 44-45 0.21529458556891967 1104 46-47 0.1361426501285763 1104 48-49 0.0531016820085668 1104 50-51 0.01050961105926973 1104 52-53 0.01936113921827598 1104 54-55 -0.09521292572695472 1104 56-57 0.08463015012832642 1104 58-59 0.015000148996833218 1104 60-61 0.043065251210641975 1104 62-63 -0.035096068905751565 1104 64-65 -0.04450194554486586 1104 66-67 -0.16324763683827825 1104 68-69 -0.1658294103933784 1104 70-71 -0.10390158779591019 1104 72-73 0.16119374574189038 1104 74-75 -0.055414582379260224 1104 76-77 -0.17544933214033165 1104 78-79 -0.18008298931718159 1104 80-81 -0.11623575017867083 1104 82-83 -0.054033432010534455 1104 84-85 0.008473047001213985 1104 86-87 -0.007516000448692495 1104 88-89 0.020928731194960193 1104 90-91 -0.002101427844131365 1104 92-93 -0.05299799176857789 1104 94-95 0.10940565108290201 1104 96-97 0.21122030732644959 1104 98-99 0.16507175746446023 1104 100-101 0.21610364697139062 1105 1 0.2966820481111725 1105 2 0.18771974588015894 1105 3 0.16933025645831634 1105 4 0.09084468019733549 1105 5 0.034133447587045396 1105 6 0.09333112558213941 1105 7 0.05471219158756213 1105 8 0.10405822794778885 1105 9 0.10916762124419677 1105 10-11 0.18579934442335144 1105 12-13 0.14397747547756978 1105 14-15 0.1458401520389785 1105 16-17 0.09249116338619245 1105 18-19 0.18168562042003344 1105 20-21 0.01793960951496132 1105 22-23 0.09645631232194773 1105 24-25 0.0985575988052716 1105 26-27 0.10588882283526857 1105 28-29 0.07448535717541205 1105 30-31 0.011761081562610798 1105 32-33 0.09835406868006658 1105 34-35 0.22379420435832031 1105 36-37 0.1496656573727151 1105 38-39 0.17199303898895835 1105 40-41 0.16438279794270727 1105 42-43 0.09604409288098026 1105 44-45 0.1605841958195029 1105 46-47 0.05506011551983647 1105 48-49 0.049036266481998325 1105 50-51 0.19429828615945155 1105 52-53 0.29532042693572436 1105 54-55 -0.012679680669819504 1105 56-57 0.08798099210461174 1105 58-59 0.20294151578238484 1105 60-61 0.05148075254739837 1105 62-63 -0.058645886887234155 1105 64-65 0.06314867912742272 1105 66-67 0.07113845379664951 1105 68-69 0.11485438126479863 1105 70-71 0.07727127422798219 1105 72-73 0.12515602198593712 1105 74-75 0.037229486353439256 1105 76-77 -0.036391989658454804 1105 78-79 0.017470857686344488 1105 80-81 -0.016167241803227483 1105 82-83 -0.02749270097724832 1105 84-85 0.11551887516673176 1105 86-87 -0.11129945602547053 1105 88-89 -0.11123166397512207 1105 90-91 0.1470602607858993 1105 92-93 0.13779331661695693 1105 94-95 0.1831823870772915 1105 96-97 0.3382985708581714 1105 98-99 0.31752164356225876 1105 100-101 0.1427609905442182 1106 1 0.1001491995040169 1106 2 0.10704445827558118 1106 3 0.09514538088250646 1106 4 0.05003921458720839 1106 5 -0.023599824003596837 1106 6 0.023448309977361248 1106 7 0.029450677901074584 1106 8 0.03331233942328993 1106 9 0.12365446003011726 1106 10-11 0.016568023602125947 1106 12-13 -0.24177439148998303 1106 14-15 -0.0018550372150301087 1106 16-17 0.03896068542176323 1106 18-19 0.08720384430409212 1106 20-21 0.16054845691952124 1106 22-23 0.1807755991939075 1106 24-25 0.18838387838214743 1106 26-27 0.16835871463581498 1106 28-29 0.13493847477795384 1106 30-31 0.18437186418572793 1106 32-33 0.17317212357806966 1106 34-35 -0.18473051978664756 1106 36-37 0.07964556220143493 1106 38-39 0.10941040342862607 1106 40-41 -0.060704459679804756 1106 42-43 -0.12220363582902394 1106 44-45 0.06935540114964311 1106 46-47 0.016315916855823787 1106 48-49 0.043959341544287156 1106 50-51 0.17917619460288137 1106 52-53 0.3579414212924874 1106 54-55 0.2585294507971767 1106 56-57 0.21328060540648153 1106 58-59 0.34994071750473665 1106 60-61 0.4306089020292134 1106 62-63 0.24520511165098924 1106 64-65 0.02536465988581682 1106 66-67 -0.004194593861562623 1106 68-69 0.38787717537481825 1106 70-71 0.27372999705285395 1106 72-73 0.25644487754606615 1106 74-75 0.12660159432481422 1106 76-77 0.17397922673007926 1106 78-79 0.1634653008444822 1106 80-81 0.10612414336401343 1106 82-83 0.004121254022010135 1106 84-85 -0.1206281565190146 1106 86-87 0.20066555107608863 1106 88-89 0.2383939921849212 1106 90-91 0.057252383064064105 1106 92-93 -0.18814846095040494 1106 94-95 0.08899002980830417 1106 96-97 0.005422922473563574 1106 98-99 -0.07243794147159122 1106 100-101 -0.6029136231896146 1107 1 0.2510418020056093 1107 2 0.20097992199429626 1107 3 0.06543349898172579 1107 4 0.010522286036149353 1107 5 0.04814721876255135 1107 6 0.0390826043834025 1107 7 0.12867419181466744 1107 8 0.04259248555342765 1107 9 0.07528229673377496 1107 10-11 0.1818307115273754 1107 12-13 0.18261438460226032 1107 14-15 0.06643168545008393 1107 16-17 0.12356728164899522 1107 18-19 0.2116123126538767 1107 20-21 0.16819923849843121 1107 22-23 0.2236099940983678 1107 24-25 0.16610887940071706 1107 26-27 0.1660435162872531 1107 28-29 0.12957360780254845 1107 30-31 0.18371624781806162 1107 32-33 0.13600027128357794 1107 34-35 0.14907183445669858 1107 36-37 0.1978609004329357 1107 38-39 0.2699465251403623 1107 40-41 0.12775536811479782 1107 42-43 0.2268157406733664 1107 44-45 -0.021329651747834077 1107 46-47 -0.021100730571276927 1107 48-49 -0.09938841014313482 1107 50-51 -0.12934562985082465 1107 52-53 -0.028789646978530925 1107 54-55 -0.07542034073651394 1107 56-57 -0.05501850980002132 1107 58-59 -0.058625329648037905 1107 60-61 -0.057155330719687925 1107 62-63 -0.12965899479585374 1107 64-65 -0.005576545144528211 1107 66-67 -0.11582796476862711 1107 68-69 -0.001594766553388638 1107 70-71 -0.10563973187304043 1107 72-73 0.01566569226229575 1107 74-75 -0.13562670344928307 1107 76-77 -0.18219005199005878 1107 78-79 -0.1896284207205774 1107 80-81 -0.1585586415286322 1107 82-83 -0.1379347025463744 1107 84-85 0.06978765568475254 1107 86-87 -0.04248035497147029 1107 88-89 -0.259843126362469 1107 90-91 -0.15357352040313188 1107 92-93 -0.2627566529295464 1107 94-95 -0.11286401321588713 1107 96-97 -0.03909931959181634 1107 98-99 0.06321234440566315 1107 100-101 0.1752981670403102 1108 1 0.16628431867676596 1108 2 0.02168653961936684 1108 3 -0.012585748847783407 1108 4 -0.05021154369381975 1108 5 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-0.4218511118667081 2316 66-67 -0.4565537160199895 2316 68-69 -0.2821032690785117 2316 70-71 -0.24115471520898524 2316 72-73 -0.25962774877135075 2316 74-75 -0.22958764870805481 2316 76-77 -0.1473574827888129 2316 78-79 -0.07804086785980857 2316 80-81 -0.3157519059656231 2316 82-83 -0.4364073873132668 2316 84-85 -0.39036313595111594 2316 86-87 -0.4251694341051895 2316 88-89 -0.5053876855926625 2316 90-91 -0.3314245638043367 2316 92-93 -0.2506353898963596 2316 94-95 -0.3356267956274088 2316 96-97 -0.3701558908788698 2316 98-99 -0.1817462816997768 2316 100-101 5.822119872078702E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 14.0 5 67.0 6 95.0 7 185.0 8 549.0 9 1025.0 10 1864.0 11 3150.0 12 4982.0 13 7306.0 14 9577.0 15 13495.0 16 18866.0 17 27302.0 18 24419.0 19 13767.0 20 11756.0 21 11879.0 22 12554.0 23 13896.0 24 15911.0 25 18922.0 26 23393.0 27 28944.0 28 36656.0 29 45626.0 30 58156.0 31 72462.0 32 93570.0 33 123653.0 34 170746.0 35 244626.0 36 374058.0 37 548455.0 38 384542.0 39 26493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27265953483421 19.54785328567861 15.284761542146184 21.894725637340997 2 15.540025272526995 20.65752778688555 40.29113754615798 23.51130939442948 3 19.832785502891973 24.736120378064406 29.941464692566832 25.48962942647679 4 12.905116683838393 16.948598201614512 36.98053475095263 33.16575036359446 5 13.420532993281686 37.87558630653297 33.930102678889526 14.773778021295817 6 31.80620310523666 36.477893711343164 17.07798294698666 14.637920236433521 7 28.66469911623966 33.33860014654294 20.016373512568716 17.980327224648683 8 26.541054536076985 35.42307109788495 19.05627166932054 18.97960269671752 9 26.450672746697666 17.084982623609786 20.252929835405265 36.211414794287286 10-11 25.047708322247104 27.31394572999259 26.495638505589508 21.1427074421708 12-13 26.10577700455406 24.435982193112622 27.820518855856317 21.637721946476997 14-15 22.733128118642473 25.616503681993642 26.66659844369763 24.98376975566626 16-17 21.58174271481025 29.267878852380502 27.196854648235547 21.953523784573694 18-19 21.49520870088458 27.309750017396855 30.26787066562422 20.927170616094344 20-21 22.243560092837818 26.090598738420855 30.61363831729411 21.052202851447213 22-23 22.012831171404162 26.153325662301874 30.48244402173268 21.351399144561285 24-25 21.87373168951084 26.337836673435085 30.677175210691765 21.111256426362317 26-27 21.65197096868804 26.52884414059486 30.64796494891317 21.171219941803933 28-29 21.274984941340303 26.81243735792004 30.76733362765005 21.145244073089607 30-31 21.532519842650544 26.39713136059796 30.733574296859974 21.336774499891526 32-33 21.40441757369104 26.525295030229596 30.641739358240176 21.42854803783919 34-35 21.92100189523408 26.43264141598137 30.495810427471483 21.150546261313075 36-37 21.798568955001492 26.26933609499912 30.583797590637623 21.34829735936176 38-39 21.560825552503715 26.681068535430235 30.587890968779806 21.170214943286243 40-41 21.791792035592938 26.648243634301306 30.39227264177647 21.167691688329285 42-43 21.674170615143648 26.61494550733324 30.113983872037416 21.5969000054857 44-45 21.71747913400492 26.691138245659996 29.94772756112437 21.643655059210715 46-47 21.94431368375379 27.078596953707983 29.413562180460666 21.563527182077554 48-49 22.062571378281355 27.20332218570019 29.10395952467693 21.630146911341523 50-51 21.951968300879667 27.29270109743468 29.038260805494954 21.717069796190703 52-53 22.197452731194556 27.606893987010476 28.6034066327885 21.592246649006466 54-55 21.916621417699105 27.34340701905123 28.723676204187083 22.016295359062582 56-57 22.030356492302403 27.62586114442666 28.70174418842638 21.642038174844554 58-59 22.29108421306852 27.322787426779698 28.65622582348535 21.729902536666433 60-61 22.06523207407377 27.30594317572463 29.204677093865257 21.424147656336345 62-63 22.004424941771696 27.345321473452394 29.122195524300338 21.52805806047557 64-65 21.9332251180377 27.373080179674776 28.947946341532305 21.745748360755222 66-67 21.869814201566125 27.46316983016574 28.68661915619103 21.980396812077103 68-69 21.759784197104345 27.434004510902714 28.867648804528912 21.93856248746403 70-71 21.920490222966308 27.525737115068953 28.656676095080986 21.897096566883754 72-73 22.054527890231974 27.369267735584145 28.689566388453397 21.886637985730484 74-75 21.832994265177224 27.87885238050406 28.320200411793838 21.967952942524878 76-77 21.92378539237076 27.578480292430935 28.41205581730435 22.08567849789396 78-79 21.974359079317388 27.59116976467169 28.428470263654486 22.006000892356433 80-81 21.848569569008216 27.856973274334106 28.40004175245705 21.894415404200622 82-83 21.967498311467693 27.749749278536196 28.463640270983852 21.819112139012258 84-85 21.901444512803888 27.65266362364811 28.29503771844833 22.15085414509967 86-87 21.874480652648213 27.54395264780165 28.46422592172642 22.117340777823717 88-89 21.939647232671707 27.54161942226061 28.317785318689953 22.200948026377727 90-91 21.877914321904075 27.48526412404746 28.45344331098901 22.18337824305946 92-93 22.05391078318 27.43090762098714 28.46217137243264 22.05301022340023 94-95 21.87566773230944 27.339263273801972 28.43266597625022 22.352403017638366 96-97 21.659823902872326 27.419841422530773 28.484733746218744 22.435600928378165 98-99 21.776783800970676 27.432763807000306 28.143547734458103 22.646904657570918 100-101 21.635434900493077 27.22632397666589 28.607889766239232 22.530351356601802 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 534.0 1 393.5 2 355.0 3 487.0 4 443.0 5 414.0 6 803.0 7 1911.5 8 3342.5 9 4109.5 10 3856.5 11 3510.5 12 3680.0 13 4001.5 14 4334.0 15 4592.0 16 4805.0 17 5126.5 18 5293.0 19 5544.0 20 6076.0 21 6760.5 22 7702.5 23 9058.0 24 10937.0 25 13260.0 26 15991.5 27 18928.0 28 21442.0 29 24377.5 30 28297.5 31 32354.0 32 36601.5 33 41636.5 34 48152.5 35 53747.5 36 58974.0 37 64321.5 38 67842.0 39 70695.5 40 74305.0 41 78928.5 42 83211.0 43 87638.5 44 92116.5 45 94933.5 46 95228.5 47 99037.5 48 108157.5 49 106185.5 50 96520.0 51 89373.5 52 82715.5 53 78815.0 54 74861.5 55 68255.5 56 60244.5 57 52777.0 58 46403.5 59 40323.5 60 33892.5 61 26955.0 62 20445.0 63 15210.5 64 11279.0 65 8613.5 66 6427.5 67 4819.5 68 3319.5 69 2062.0 70 1418.0 71 1014.0 72 785.0 73 645.5 74 529.0 75 415.5 76 272.5 77 141.5 78 80.0 79 56.0 80 39.5 81 32.5 82 25.0 83 13.5 84 8.0 85 5.5 86 3.5 87 1.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016373512568717587 2 4.093378142179396E-5 3 0.0 4 4.093378142179396E-5 5 0.040442576044732434 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003888709235070427 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.321391584833216E-4 24-25 2.2513579781986682E-4 26-27 8.800763005685703E-4 28-29 9.619438634121582E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.526060491942186E-4 42-43 0.010479048043979254 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.046689071089698E-5 54-55 1.8420201639807282E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.046689071089698E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.186756284358793E-4 84-85 4.093378142179396E-5 86-87 0.0 88-89 0.0 90-91 0.011952664175163837 92-93 0.0018420201639807285 94-95 0.0 96-97 0.0 98-99 0.0013712816776300977 100-101 0.018481602311939977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2442970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.48300863619528 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88033393607363 37.29701536139796 2 14.187513251209655 14.32456707973904 3 4.8331154150792806 7.319706217175276 4 2.2373242323973037 4.5178743418432825 5 1.2620204782600908 3.1855295351529733 6 0.7591697877885871 2.299510497196187 7 0.5046786638978822 1.78343881436422 8 0.3674706073516837 1.4840817475586379 9 0.2767347383325335 1.2573361966658876 >10 1.51490989315534 14.167880607589666 >50 0.11525543736518926 4.0278841291838825 >100 0.057819147364627635 5.169810851923163 >500 0.0024362744827136074 0.8737242686948126 >1k 9.745097930850162E-4 0.8763480652972175 >5k 8.120914942375135E-5 0.3287535149522075 >10k+ 1.624182988475027E-4 1.0865387712655388 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14592 0.5973057385068176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11911 0.4875622705149879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8019 0.3282479932213658 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4538 0.185757500092101 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3025 0.12382468880092674 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.093378142179397E-5 0.0 9 0.0 0.0 0.0 4.093378142179397E-5 0.0 10-11 0.0 4.093378142179397E-5 0.0 4.093378142179397E-5 0.0 12-13 0.0 4.093378142179397E-5 0.0 4.093378142179397E-5 0.0 14-15 0.0 8.186756284358793E-5 0.0 4.093378142179397E-5 0.0 16-17 0.0 8.186756284358793E-5 0.0 4.093378142179397E-5 0.0 18-19 0.0 8.186756284358793E-5 0.0 4.093378142179397E-5 0.0 20-21 0.0 8.186756284358793E-5 0.0 4.093378142179397E-5 0.0 22-23 0.0 8.186756284358793E-5 0.0 4.093378142179397E-5 0.0 24-25 0.0 8.186756284358793E-5 0.0 1.0233445355448492E-4 0.0 26-27 0.0 8.186756284358793E-5 0.0 2.2513579781986682E-4 0.0 28-29 0.0 8.186756284358793E-5 0.0 7.982087377249824E-4 0.0 30-31 0.0 8.186756284358793E-5 0.0 0.0021694904153550802 0.0 32-33 0.0 8.186756284358793E-5 0.0 0.0037045072186723538 0.0 34-35 0.0 8.186756284358793E-5 0.0 0.005546527382653082 0.0 36-37 0.0 8.186756284358793E-5 0.0 0.007900219814406235 0.0 38-39 0.0 8.186756284358793E-5 0.0 0.010601849388244638 0.0 40-41 0.0 8.186756284358793E-5 0.0 0.01418355526265161 0.0 42-43 0.0 8.186756284358793E-5 0.0 0.019177476596110472 0.0 44-45 0.0 8.186756284358793E-5 0.0 0.026115752547104548 0.0 46-47 0.0 8.186756284358793E-5 0.0 0.0399923044490927 0.0 48-49 0.0 8.186756284358793E-5 0.0 0.06485957666283254 0.0 50-51 0.0 8.186756284358793E-5 0.0 0.08530600048301862 0.0 52-53 0.0 8.186756284358793E-5 0.0 0.10452441086055089 0.0 54-55 0.0 8.186756284358793E-5 0.0 0.1288390770250965 0.0 56-57 0.0 8.186756284358793E-5 0.0 0.15213039865409728 0.0 58-59 0.0 8.186756284358793E-5 0.0 0.18156178749636712 0.0 60-61 0.0 8.186756284358793E-5 0.0 0.22724388756308916 0.0 62-63 0.0 8.186756284358793E-5 0.0 0.2658853772252627 0.0 64-65 0.0 8.186756284358793E-5 0.0 0.3097663909094258 0.0 66-67 0.0 8.186756284358793E-5 0.0 0.37972222335927175 0.0 68-69 0.0 8.186756284358793E-5 0.0 0.4556953216781213 0.0 70-71 0.0 8.186756284358793E-5 0.0 0.5283118499203838 0.0 72-73 0.0 8.186756284358793E-5 0.0 0.6057176305889962 0.0 74-75 0.0 8.186756284358793E-5 0.0 0.6852110341101201 0.0 76-77 0.0 1.228013442653819E-4 0.0 0.7624735465437562 0.0 78-79 0.0 1.228013442653819E-4 0.0 0.8508291137426984 0.0 80-81 0.0 1.228013442653819E-4 0.0 0.9808962042104488 0.0 82-83 0.0 1.228013442653819E-4 0.0 1.1247784459080545 0.0 84-85 0.0 1.228013442653819E-4 0.0 1.2702571050811102 0.0 86-87 0.0 1.228013442653819E-4 0.0 1.470791700266479 0.0 88-89 0.0 1.228013442653819E-4 0.0 1.7261570956663406 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11900 0.0 29.354021 1 GTATCAA 17020 0.0 29.263681 1 GTACATG 11980 0.0 25.230597 1 ATCAACG 20560 0.0 24.197515 3 TATCAAC 20650 0.0 24.138075 2 TACATGG 12260 0.0 24.10722 2 TCAACGC 20700 0.0 24.010904 4 CAACGCA 20965 0.0 23.752733 5 AACGCAG 21475 0.0 23.179148 6 ACATGGG 12290 0.0 23.159124 3 GAGTACT 11905 0.0 22.604414 12-13 GGACCGA 425 0.0 22.351694 6 TACCTGG 2105 0.0 22.347569 2 GTACCTG 2125 0.0 21.694021 1 AGTACTT 12470 0.0 21.618328 12-13 GTACTTT 12695 0.0 21.08291 14-15 ACGCAGA 23905 0.0 20.746004 7 ACCGGTC 115 0.0037230155 20.653557 8 AGAGTAC 18915 0.0 20.606058 10-11 CGCAGAG 23895 0.0 20.595646 8 >>END_MODULE