FastQCFastQC Report
Thu 26 May 2016
SRR937638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490843
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119250.4787535786077244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91380.3668637485381455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60520.2429699503340837No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41270.16568687789635878No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32740.13144144372005784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159900.029.6489091
GGTATCA111150.028.3211271
GTACATG125500.026.2573871
TACATGG127500.025.5016332
ATCAACG190350.024.5968323
TCAACGC191300.024.5024914
TATCAAC192100.024.3989772
CAACGCA192050.024.3563185
ACATGGG128300.024.3037343
AACGCAG195750.023.9081926
GAGTACT99100.022.72088212-13
TACCTGG19650.021.2883592
ACGCAGA221000.021.15517
CGCAGAG221950.020.9789248
CATGGGA73650.020.9132024
AGTACTT106150.020.71960412-13
TCGCGCA1150.003719020220.6574029
GTACTTT107400.020.6549614-15
AGAGTAC173150.020.08250210-11
CATGGGG52650.019.8642714
Produced by FastQC (version 0.11.5)