##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937638_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2490843 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.590396102845503 33.0 31.0 34.0 30.0 34.0 2 31.705738579268143 33.0 31.0 34.0 30.0 34.0 3 31.752127693315074 33.0 31.0 34.0 30.0 34.0 4 35.34465118837277 37.0 35.0 37.0 33.0 37.0 5 35.328255534371294 37.0 35.0 37.0 33.0 37.0 6 35.42261876802352 37.0 35.0 37.0 33.0 37.0 7 35.352822719055354 37.0 35.0 37.0 33.0 37.0 8 35.343885182647 37.0 35.0 37.0 33.0 37.0 9 36.93007387458784 39.0 37.0 39.0 33.0 39.0 10-11 36.814277134287465 39.0 37.0 39.0 33.0 39.0 12-13 36.684216749108636 39.0 37.0 39.0 32.0 39.0 14-15 37.78490013220424 40.0 37.5 41.0 32.5 41.0 16-17 37.72553388551587 40.0 37.0 41.0 32.0 41.0 18-19 37.68128179897328 40.0 37.0 41.0 32.0 41.0 20-21 37.52613151451135 40.0 37.0 41.0 32.0 41.0 22-23 37.37242993637094 40.0 37.0 41.0 32.0 41.0 24-25 37.22161031425907 39.0 37.0 41.0 31.5 41.0 26-27 37.32241574438854 40.0 37.0 41.0 32.0 41.0 28-29 37.26365029831266 40.0 37.0 41.0 31.5 41.0 30-31 37.060503211161844 39.0 36.5 41.0 31.0 41.0 32-33 36.8221592047351 39.0 36.0 41.0 30.5 41.0 34-35 36.55857474758545 39.0 35.5 40.5 30.0 41.0 36-37 36.56976734382697 39.0 35.5 41.0 30.0 41.0 38-39 36.52307411587161 39.0 35.0 41.0 30.0 41.0 40-41 36.33170878293012 39.0 35.0 41.0 30.0 41.0 42-43 36.08177171343196 39.0 35.0 41.0 29.5 41.0 44-45 35.82960848997709 39.0 35.0 40.5 28.5 41.0 46-47 35.452770608183656 39.0 35.0 40.0 26.5 41.0 48-49 35.103204617874354 39.0 35.0 40.0 25.5 41.0 50-51 34.20274983208496 37.5 33.5 39.5 23.5 40.5 52-53 34.31836289962876 38.0 33.5 39.5 24.0 40.5 54-55 34.622619530817474 38.0 34.0 40.0 24.0 41.0 56-57 34.34119854201971 38.0 34.0 40.0 23.0 41.0 58-59 34.10177297404935 38.0 34.0 40.0 22.5 41.0 60-61 33.77678320151049 38.0 33.0 40.0 22.0 41.0 62-63 33.408373791523594 37.0 33.0 40.0 20.5 41.0 64-65 33.0312717421371 36.5 32.0 39.5 19.0 41.0 66-67 32.604149077240116 36.0 32.0 39.0 17.5 41.0 68-69 32.11100298172145 35.5 31.0 39.0 13.5 40.0 70-71 31.654472802982767 35.0 31.0 38.5 9.5 40.0 72-73 31.168651536849172 35.0 30.5 37.5 7.5 40.0 74-75 30.6641181318935 35.0 30.0 37.0 6.0 39.0 76-77 30.190835994079116 34.0 29.5 36.5 2.0 39.0 78-79 29.64134632331303 34.0 29.0 36.0 2.0 38.5 80-81 29.095581696638448 34.0 29.0 35.5 2.0 37.0 82-83 28.566199274703383 34.0 28.0 35.0 2.0 37.0 84-85 28.073702156257944 34.0 26.5 35.0 2.0 36.0 86-87 27.529861777719432 33.0 26.0 35.0 2.0 36.0 88-89 27.02591712926106 33.0 25.5 35.0 2.0 35.5 90-91 26.47677834371737 32.5 25.0 35.0 2.0 35.0 92-93 25.883354551049585 32.0 23.5 34.5 2.0 35.0 94-95 25.216524285151653 32.0 19.5 34.0 2.0 35.0 96-97 24.400790214397293 31.5 12.5 34.0 2.0 35.0 98-99 23.43364876871003 31.0 2.0 34.0 2.0 35.0 100-101 20.925880916621402 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41959457678693113 1101 2 0.5624890321721558 1101 3 0.5445400190903982 1101 4 0.4786188709206485 1101 5 0.40957128261300113 1101 6 0.48838238173691195 1101 7 0.47689424979368056 1101 8 0.4876202682806934 1101 9 0.6393119735955253 1101 10-11 0.5726444323947746 1101 12-13 0.6692651538348642 1101 14-15 0.9151349978556524 1101 16-17 0.9598799391390145 1101 18-19 0.9148654670082621 1101 20-21 0.9829854974181487 1101 22-23 0.9175203153246159 1101 24-25 0.8572513230839149 1101 26-27 0.892227048925541 1101 28-29 0.791814775449545 1101 30-31 0.7939500933435326 1101 32-33 0.8647861993525936 1101 34-35 0.9013219555333194 1101 36-37 0.7894403805454004 1101 38-39 0.8083613187091103 1101 40-41 0.9104216787479089 1101 42-43 0.9017891431899017 1101 44-45 0.990936315411794 1101 46-47 1.0603288991706492 1101 48-49 1.087793859781108 1101 50-51 1.0886937681310087 1101 52-53 1.0909961953692857 1101 54-55 1.2819387285384778 1101 56-57 1.3386838404932817 1101 58-59 1.2521260187515466 1101 60-61 1.3348316221521657 1101 62-63 1.2424740258058975 1101 64-65 1.2276615485129625 1101 66-67 1.3619963790824343 1101 68-69 1.4090355485120867 1101 70-71 1.4362509034675561 1101 72-73 1.4963744722148498 1101 74-75 1.5386197621533206 1101 76-77 1.468108453842536 1101 78-79 1.5647959038077204 1101 80-81 1.5294640644592938 1101 82-83 1.682673868027976 1101 84-85 1.6782500373829912 1101 86-87 1.7430294366921686 1101 88-89 1.8154909952731337 1101 90-91 2.0578309073334964 1101 92-93 2.226590362796397 1101 94-95 2.2040716089569408 1101 96-97 2.5418420438451115 1101 98-99 3.037458595818528 1101 100-101 3.0861420461115934 1102 1 0.2329001486522273 1102 2 0.3189807308143422 1102 3 0.2736429062397434 1102 4 0.18303831752567845 1102 5 0.28139079262741973 1102 6 0.22626508592345118 1102 7 0.2509701373987383 1102 8 0.32651528385498807 1102 9 0.31910358925663473 1102 10-11 0.36130375714979124 1102 12-13 0.41693749484738163 1102 14-15 0.5880841049604655 1102 16-17 0.5398010457267333 1102 18-19 0.50724330463256 1102 20-21 0.47572952787198375 1102 22-23 0.6125041849294988 1102 24-25 0.6829977375285523 1102 26-27 0.6782131407478289 1102 28-29 0.5643451494854972 1102 30-31 0.6479185605335047 1102 32-33 0.5589110089619354 1102 34-35 0.6554630652554039 1102 36-37 0.7018996754012221 1102 38-39 0.8370360119355027 1102 40-41 0.7727956814763886 1102 42-43 0.8404270185276204 1102 44-45 0.8082418063903987 1102 46-47 0.8040731802287908 1102 48-49 0.8639850788085468 1102 50-51 0.6894040182803209 1102 52-53 0.8412031064148664 1102 54-55 0.8323589421226743 1102 56-57 0.982503266732202 1102 58-59 1.0915343398254933 1102 60-61 1.1296376132560795 1102 62-63 1.1923511791432233 1102 64-65 1.0634554261126254 1102 66-67 1.0175761524985916 1102 68-69 1.0668225536317877 1102 70-71 1.1988244507750672 1102 72-73 1.082663239147795 1102 74-75 1.117279000244018 1102 76-77 1.0157545066803237 1102 78-79 1.0825481854262904 1102 80-81 1.2233270756252672 1102 82-83 1.2770375822581848 1102 84-85 1.316721011699297 1102 86-87 1.4013992593119866 1102 88-89 1.4552453236138803 1102 90-91 1.5633261469350437 1102 92-93 1.7910604863580382 1102 94-95 1.9259470535629184 1102 96-97 1.9173964246177668 1102 98-99 2.1585438063369757 1102 100-101 2.1765879917745146 1103 1 0.28491076646951896 1103 2 0.36019327154017944 1103 3 0.22899718885209097 1103 4 0.1967905952370188 1103 5 0.24588785233255805 1103 6 0.20454841518805722 1103 7 0.2940063922799112 1103 8 0.23242687826837738 1103 9 0.25281742527789675 1103 10-11 0.32721662883262326 1103 12-13 0.3129103363907788 1103 14-15 0.4173621078054808 1103 16-17 0.3862475070208333 1103 18-19 0.3121924147156818 1103 20-21 0.3068683268884911 1103 22-23 0.4610838922081726 1103 24-25 0.3049058956751054 1103 26-27 0.11571552275233898 1103 28-29 0.3007321868487196 1103 30-31 0.3346561259549361 1103 32-33 0.31335183790979926 1103 34-35 0.3064535671089814 1103 36-37 0.42956394100733775 1103 38-39 0.5741088090834765 1103 40-41 0.5099524281887042 1103 42-43 0.566150916135939 1103 44-45 0.44489289861028425 1103 46-47 0.5508640407478751 1103 48-49 0.5119964011841418 1103 50-51 0.3550063795807077 1103 52-53 0.5200156784544134 1103 54-55 0.554589147278925 1103 56-57 0.6462947895773041 1103 58-59 0.6117829232409662 1103 60-61 0.5899117008582238 1103 62-63 0.615613809432439 1103 64-65 0.6596925285238839 1103 66-67 0.8746924489116239 1103 68-69 0.9070343434468029 1103 70-71 1.0472110225529754 1103 72-73 1.126500620086194 1103 74-75 1.0648396216150005 1103 76-77 0.957159513370538 1103 78-79 0.924505920168329 1103 80-81 1.0231717431798408 1103 82-83 0.7815747214424427 1103 84-85 0.9402749418264804 1103 86-87 0.8305687457569633 1103 88-89 0.9592138718838505 1103 90-91 1.0178973294702338 1103 92-93 1.091304558128222 1103 94-95 0.9938717791302487 1103 96-97 1.0998023470277687 1103 98-99 1.4978468292423308 1103 100-101 1.7545143036280884 1104 1 0.24805268500177036 1104 2 0.25651608979608653 1104 3 0.32187817392638607 1104 4 0.2823447198246001 1104 5 0.35613023395040244 1104 6 0.37000687804429333 1104 7 0.3987415769407505 1104 8 0.3754541569833876 1104 9 0.39103269360469284 1104 10-11 0.5224176595355132 1104 12-13 0.5190098120069777 1104 14-15 0.5271655577576269 1104 16-17 0.5242369665716353 1104 18-19 0.5121476081653 1104 20-21 0.704619322223131 1104 22-23 0.6321550985953692 1104 24-25 0.545379048787666 1104 26-27 0.5721377273158055 1104 28-29 0.4759762886727472 1104 30-31 0.43519126738227243 1104 32-33 0.4501079755953086 1104 34-35 0.5772137663078496 1104 36-37 0.4720964194134254 1104 38-39 0.6123790834132379 1104 40-41 0.740353241303275 1104 42-43 0.6354909557362021 1104 44-45 0.6698296467812312 1104 46-47 0.5313678632591277 1104 48-49 0.5675088247263105 1104 50-51 0.48695355149070707 1104 52-53 0.3934031754478582 1104 54-55 0.5159979200259883 1104 56-57 0.648061933537754 1104 58-59 0.6202208136491265 1104 60-61 0.7405616749282729 1104 62-63 0.591173942055768 1104 64-65 0.6007511099170344 1104 66-67 0.5980792354954403 1104 68-69 0.8014116541809884 1104 70-71 0.778007635319856 1104 72-73 0.7902442048334954 1104 74-75 0.7182234041809394 1104 76-77 0.7729779509361876 1104 78-79 0.6513488496134876 1104 80-81 0.8560123566896003 1104 82-83 1.0608060480715409 1104 84-85 1.0810116569126365 1104 86-87 1.0672249694020337 1104 88-89 1.1124766310945624 1104 90-91 1.2209184479188657 1104 92-93 1.362206331818097 1104 94-95 1.3940346151666922 1104 96-97 1.4325280264077236 1104 98-99 1.632125413335043 1104 100-101 1.6036080746549466 1105 1 0.1477188402859042 1105 2 0.2386088275068019 1105 3 0.2068289352816386 1105 4 0.26161795190682113 1105 5 0.2668128047453351 1105 6 0.3093699241489105 1105 7 0.28808641572378946 1105 8 0.25584191377564025 1105 9 0.31551404786132764 1105 10-11 0.34526189951458264 1105 12-13 0.3720985822305849 1105 14-15 0.26304814616830896 1105 16-17 0.41338937960044575 1105 18-19 0.4516315982110868 1105 20-21 0.558021790794136 1105 22-23 0.5225600044497156 1105 24-25 0.530469914496507 1105 26-27 0.4595566652965388 1105 28-29 0.3791578702251144 1105 30-31 0.32441351896407156 1105 32-33 0.4676083666912021 1105 34-35 0.5686152436643752 1105 36-37 0.5271689660590084 1105 38-39 0.5692050542841471 1105 40-41 0.5967512086563005 1105 42-43 0.4048216098983204 1105 44-45 0.48036800507239974 1105 46-47 0.4783599085657855 1105 48-49 0.40342717047611387 1105 50-51 0.35935867951381084 1105 52-53 0.29244308431922406 1105 54-55 0.3652016947915655 1105 56-57 0.43612493087041315 1105 58-59 0.649837618546826 1105 60-61 0.5232793115784915 1105 62-63 0.5910889855101331 1105 64-65 0.6068781694148768 1105 66-67 0.6988965273239671 1105 68-69 0.5808845094965349 1105 70-71 0.6259960549374917 1105 72-73 0.7330165226058121 1105 74-75 0.6535231611099128 1105 76-77 0.5952534586049545 1105 78-79 0.5845988039308594 1105 80-81 0.7119963868309327 1105 82-83 0.8284998508777015 1105 84-85 0.9384350185494803 1105 86-87 0.9561119959295112 1105 88-89 0.5742200577256327 1105 90-91 0.8577602729629383 1105 92-93 0.930924510086836 1105 94-95 1.1648003283481323 1105 96-97 1.1203661271671699 1105 98-99 1.4161662029938107 1105 100-101 1.4714575927853595 1106 1 0.09138147569898436 1106 2 0.19794526280420754 1106 3 0.217621690722531 1106 4 0.19523353797657705 1106 5 0.21774794293653343 1106 6 0.1392780999470915 1106 7 0.1373725524138294 1106 8 0.1561661903270064 1106 9 0.2637016083788737 1106 10-11 -0.008239994806352513 1106 12-13 -0.08053575859121764 1106 14-15 0.12667117305271347 1106 16-17 0.23392521573356362 1106 18-19 0.1005235991728668 1106 20-21 0.2126922829276694 1106 22-23 0.1083566590074625 1106 24-25 0.01538471879333514 1106 26-27 0.05425549101251903 1106 28-29 0.27463538206868776 1106 30-31 0.32406877204925166 1106 32-33 0.41864325728848684 1106 34-35 0.21534355970079844 1106 36-37 0.4738352211522283 1106 38-39 0.37872312344148185 1106 40-41 0.2267502013844549 1106 42-43 0.33933561747559793 1106 44-45 0.26163632279843085 1106 46-47 0.7064959357556262 1106 48-49 0.8010556951152807 1106 50-51 0.7131716908667372 1106 52-53 0.7139004117345635 1106 54-55 0.7766298438158117 1106 56-57 0.7478747570347863 1106 58-59 0.5034478758235537 1106 60-61 0.24796981917851468 1106 62-63 0.6037008414247751 1106 64-65 0.6477431884880573 1106 66-67 0.7398171088122609 1106 68-69 0.7921201396263129 1106 70-71 0.696163424528276 1106 72-73 0.7470853485167446 1106 74-75 0.7470855567273027 1106 76-77 0.8998856094227925 1106 78-79 0.8548909452608484 1106 80-81 0.7465964999053227 1106 82-83 0.5934748965298624 1106 84-85 0.711226818706745 1106 86-87 0.7438482986569426 1106 88-89 1.1401009981926151 1106 90-91 1.2531797854433613 1106 92-93 1.3482944258009297 1106 94-95 1.3157184079031161 1106 96-97 1.28632228809672 1106 98-99 1.5425645237741534 1106 100-101 1.3230580177890978 1107 1 0.4192136054074034 1107 2 0.4667285386792308 1107 3 0.44132433566861806 1107 4 0.3413122485806497 1107 5 0.3992668692320507 1107 6 0.4419828050051109 1107 7 0.4487552387368865 1107 8 0.41545600212118927 1107 9 0.48597493054088403 1107 10-11 0.5458015114692145 1107 12-13 0.6620857556871513 1107 14-15 0.8039033541328848 1107 16-17 0.7021957351648425 1107 18-19 0.7718648036831013 1107 20-21 0.8416726760438493 1107 22-23 0.8179675442265477 1107 24-25 0.7752598889404112 1107 26-27 0.7472046312346734 1107 28-29 0.7561570167386762 1107 30-31 0.7731909966206771 1107 32-33 0.853311498889461 1107 34-35 0.8755818267604951 1107 36-37 0.8195923341298865 1107 38-39 0.8598862916455161 1107 40-41 0.7850837822573808 1107 42-43 0.8439803923223081 1107 44-45 0.7747480878023865 1107 46-47 0.9119201108808639 1107 48-49 0.8387555687827231 1107 50-51 0.816546150720761 1107 52-53 0.7179527515080082 1107 54-55 0.8521914820503653 1107 56-57 0.9265977672095431 1107 58-59 0.9220064439845999 1107 60-61 1.2194866311946129 1107 62-63 1.1073713003981354 1107 64-65 0.9829601817031417 1107 66-67 1.1830633839237024 1107 68-69 1.2023111411831948 1107 70-71 1.2223460668184067 1107 72-73 1.0817972735768997 1107 74-75 1.152225352533339 1107 76-77 1.1879428137862469 1107 78-79 1.114276249096779 1107 80-81 1.2128183524684069 1107 82-83 1.4828032895551857 1107 84-85 1.2937878576042472 1107 86-87 1.329086784133942 1107 88-89 1.318168669112886 1107 90-91 1.5337045635750677 1107 92-93 1.6309312991835476 1107 94-95 1.821367203375413 1107 96-97 1.7942106471537862 1107 98-99 1.8286838425218725 1107 100-101 1.7117425166262414 1108 1 0.13320476992066688 1108 2 0.1823702554481521 1108 3 0.2522780646838356 1108 4 0.12948584038196742 1108 5 0.14549009870863472 1108 6 0.11254045851024586 1108 7 0.21945981487624522 1108 8 0.19982527226006397 1108 9 0.17579590432981718 1108 10-11 0.2431106080753338 1108 12-13 0.17149356806633165 1108 14-15 0.22208854844431158 1108 16-17 0.2489791627479505 1108 18-19 0.28152908907028973 1108 20-21 0.26044864729069417 1108 22-23 0.2604175340867201 1108 24-25 0.31970771798613384 1108 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98-99 0.40147004778034656 2316 100-101 0.5142974095053532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10036.0 3 2178.0 4 2022.0 5 2291.0 6 2562.0 7 3171.0 8 3870.0 9 4737.0 10 6006.0 11 7775.0 12 10099.0 13 12734.0 14 14685.0 15 17635.0 16 22380.0 17 28966.0 18 25342.0 19 18340.0 20 17997.0 21 19246.0 22 21241.0 23 23260.0 24 26334.0 25 30023.0 26 34563.0 27 40147.0 28 48108.0 29 56689.0 30 68752.0 31 85069.0 32 107081.0 33 137084.0 34 181387.0 35 246621.0 36 346034.0 37 448042.0 38 322480.0 39 35846.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26489492285537 18.842041167430757 14.528489872451198 22.364574037262678 2 14.972131895876423 20.0531599282184 41.31841935559186 23.656288820313325 3 19.638508533739284 24.77469173320925 29.452208081391934 26.134591651659534 4 12.42034127005532 16.326482142476163 37.36717506826166 33.88600151920686 5 13.461361960305679 37.85754195341408 33.844715206281016 14.836380879999227 6 31.684731251832908 36.09417158157746 17.53188004037327 14.68921712621636 7 28.76636674130945 33.155957833731485 20.082314889043438 17.995360535915623 8 26.436931874405172 35.289735505582 19.260659381679837 19.012673238332987 9 26.581194346275538 17.26160041354979 20.365396181101016 35.79180905907366 10-11 24.97086916983621 27.198691269923163 26.672515964378825 21.157923595861803 12-13 26.00899927650982 24.40774675550759 27.744229225503464 21.839024742479133 14-15 22.79077233083106 25.567275650779735 26.52332351693505 25.118628501454154 16-17 21.745289678298793 29.303745612260375 26.909981501808588 22.040983207632245 18-19 21.648275531953836 27.574232594322527 29.672302198931565 21.105189674792072 20-21 22.515689424703332 26.405592869911352 29.779993110267693 21.298724595117623 22-23 22.136789558439542 26.48757382787965 29.7575100956175 21.618126518063306 24-25 21.994826478679034 26.589293119683234 29.948884225667214 21.466996175970518 26-27 21.724782126104905 26.809535388054158 29.95358043640946 21.512102049431476 28-29 21.41571848554919 27.133628769995475 30.05353984935305 21.397112895102286 30-31 21.648658634774023 26.718671022733787 29.992338733765067 21.640331608727127 32-33 21.491407339344722 26.83600284182569 29.954528375211105 21.718061443618485 34-35 22.04166650743562 26.83048490781327 29.73097730384361 21.3968712809075 36-37 21.871403361471284 26.621424728885223 29.821690187580757 21.685481722062736 38-39 21.63702955373007 26.954838927626625 29.901585427355958 21.506546091287348 40-41 21.85186652315009 26.9993406629203 29.69363447148573 21.455158342443877 42-43 21.699385680134966 26.886182398319068 29.49666475012222 21.917767171423737 44-45 21.728372021185542 27.06005170893693 29.33754158917298 21.874034680704554 46-47 21.93570379219118 27.373540836468102 28.873665020598477 21.817090350742237 48-49 22.086133656395056 27.399031981461096 28.661523183989697 21.85331117815415 50-51 21.936752573058445 27.571484572912823 28.59704731096762 21.894715543061103 52-53 22.143744603532117 27.782766870173354 28.281233528954036 21.79225499734049 54-55 21.943658147848343 27.478674463153556 28.40532346538088 22.17234392361722 56-57 22.083455656139535 27.87646938176378 28.24430243827893 21.795772523817757 58-59 22.261372970701284 27.53642859512757 28.289663257314125 21.91253517685702 60-61 22.094962087024218 27.448399327090673 28.821542105992265 21.635096479892844 62-63 21.976576688162634 27.56604420051249 28.757075733471453 21.700303377853416 64-65 21.87667202384531 27.626172012685917 28.60345329914873 21.893702664320045 66-67 21.86461292601141 27.69960122080762 28.288993368559197 22.146792484621777 68-69 21.679562299175995 27.673839694687185 28.545843169269254 22.100754836867566 70-71 21.873780964997156 27.756456813686814 28.29303329287645 22.07672892843958 72-73 21.98051109808151 27.52261479921147 28.421918519966248 22.074955582740767 74-75 21.649603330216284 28.05906307502036 28.200717927061024 22.09061566770233 76-77 21.869614046235625 27.801942688575622 28.053842696719588 22.274600568469165 78-79 21.982959800145956 27.78973497781645 28.127812156822607 22.099493065214993 80-81 21.892913703754644 28.0424793309992 28.031761773428016 22.03284519181814 82-83 21.979325241637092 28.000590270824937 28.071720317781224 21.948364169756744 84-85 21.92182001131878 27.888444693292268 27.97499414459287 22.214741150796083 86-87 21.921718273443204 27.81480267721253 28.12585070257729 22.137628346766977 88-89 22.018274101877193 27.750384229036694 27.955989991949487 22.275351677136623 90-91 21.988546794067496 27.769620853842937 28.049637261525596 22.192195090563974 92-93 22.161536251366993 27.697667783450274 28.084436963922254 22.05635900126048 94-95 21.92889877774228 27.67171455278686 28.048994058712363 22.350392610758497 96-97 21.826153083313795 27.673114809871397 28.070877430319157 22.42985467649565 98-99 21.952251450063986 27.713153976646147 27.798999534274277 22.53559503901559 100-101 22.132145584363727 27.210813914726078 28.288771300686456 22.36826920022374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2967.0 1 1735.0 2 530.5 3 572.5 4 488.5 5 461.5 6 786.0 7 1705.5 8 2981.0 9 3640.0 10 3468.5 11 3264.0 12 3415.5 13 3700.0 14 3902.5 15 4051.0 16 4230.5 17 4469.5 18 4672.0 19 4980.0 20 5446.5 21 6203.5 22 7227.5 23 8737.0 24 10744.0 25 13074.0 26 16030.0 27 19083.5 28 21814.0 29 24906.5 30 28828.0 31 32901.5 32 37431.0 33 42992.0 34 49546.5 35 54894.0 36 60304.5 37 65934.0 38 69181.0 39 71951.0 40 75731.5 41 80560.0 42 84597.0 43 88990.5 44 93928.0 45 96881.0 46 97633.5 47 100976.5 48 108676.0 49 107160.0 50 98281.0 51 91241.0 52 84378.0 53 80151.0 54 76312.0 55 69783.5 56 62084.5 57 54496.0 58 47939.5 59 41875.0 60 35027.0 61 28040.0 62 21635.0 63 16257.5 64 12425.0 65 9507.0 66 6833.5 67 5043.0 68 3599.0 69 2300.5 70 1656.0 71 1272.0 72 1008.0 73 799.5 74 611.0 75 466.5 76 306.5 77 186.0 78 112.0 79 79.0 80 54.5 81 33.5 82 25.0 83 21.0 84 17.0 85 13.5 86 13.0 87 11.0 88 11.0 89 10.0 90 6.5 91 4.0 92 3.5 93 3.0 94 2.5 95 2.0 96 3.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3097344955101546 2 0.26661656314749665 3 0.25971127044137265 4 0.26705818070428367 5 0.26733921005860267 6 0.20234113510967972 7 0.19190290194925974 8 0.19338834282208875 9 0.20266231151461572 10-11 0.20372620835596625 12-13 0.19979179739550024 14-15 0.20220062043252024 16-17 0.18684437357151776 18-19 0.19158172554432376 20-21 0.18204680102278625 22-23 0.19027694639927123 24-25 0.18957437301347374 26-27 0.18927327013384626 28-29 0.20197981165412673 30-31 0.19918959163624525 32-33 0.20543245800718873 34-35 0.19822606242143725 36-37 0.18389356535116827 38-39 0.17347540571605677 40-41 0.17080562685002626 42-43 0.18457606521165726 44-45 0.18543922679992275 46-47 0.18529871212276325 48-49 0.20248164978683925 50-51 0.19830635652267123 52-53 0.21763716139475672 54-55 0.18790827041286826 56-57 0.17074540627410076 58-59 0.17265239117840828 60-61 0.1617725404612013 62-63 0.1676139363259748 64-65 0.16655003948462427 66-67 0.16574709847228428 68-69 0.16913952424942078 70-71 0.17042422986916478 72-73 0.1684971714395488 74-75 0.18138437468760576 76-77 0.17425827320308826 78-79 0.17779121365738426 80-81 0.17138775908397277 82-83 0.1545259978248328 84-85 0.15358254213533332 86-87 0.1576976148235758 88-89 0.1623948197457648 90-91 0.1687380537432508 92-93 0.1641412164476043 94-95 0.16430180465007227 96-97 0.16797525978152777 98-99 0.17668716976541676 100-101 0.16098967297416977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2490843.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.46768612231625 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43125402790803 39.577033601489816 2 13.390073837990668 14.050923825726658 3 4.593552994653158 7.230392901290632 4 2.072786798569108 4.350173085832188 5 1.1424924725976586 2.997196822468148 6 0.6884676176592991 2.167338172123616 7 0.48369162092739626 1.7764726102769104 8 0.3496593693819488 1.4676654433967302 9 0.2497328809708901 1.1792615771882184 >10 1.4345362121369447 13.838023617587108 >50 0.10860430761943903 3.9199479468025245 >100 0.05192269307707652 4.739024675480976 >500 0.0022269006844661933 0.8028139098717685 >1k 7.678967877462269E-4 0.811274761074276 >5k 1.5357935754924538E-4 0.6120015703574818 >10k+ 7.678967877462269E-5 0.4804554790331119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11925 0.4787535786077244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9138 0.3668637485381455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6052 0.2429699503340837 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4127 0.16568687789635878 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3274 0.13144144372005784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.0147050616999945E-5 0.0 4 0.0 0.0 0.0 4.0147050616999945E-5 0.0 5 0.0 4.0147050616999945E-5 0.0 4.0147050616999945E-5 0.0 6 0.0 4.0147050616999945E-5 0.0 4.0147050616999945E-5 0.0 7 0.0 4.0147050616999945E-5 0.0 4.0147050616999945E-5 0.0 8 0.0 4.0147050616999945E-5 0.0 4.0147050616999945E-5 0.0 9 0.0 4.0147050616999945E-5 0.0 4.0147050616999945E-5 0.0 10-11 0.0 4.0147050616999945E-5 0.0 1.2044115185099984E-4 0.0 12-13 0.0 4.0147050616999945E-5 0.0 1.2044115185099984E-4 0.0 14-15 0.0 4.0147050616999945E-5 0.0 1.2044115185099984E-4 0.0 16-17 0.0 4.0147050616999945E-5 0.0 1.6058820246799978E-4 0.0 18-19 0.0 8.029410123399989E-5 0.0 1.6058820246799978E-4 0.0 20-21 0.0 1.2044115185099984E-4 0.0 1.6058820246799978E-4 0.0 22-23 0.0 1.2044115185099984E-4 0.0 2.4088230370199968E-4 0.0 24-25 0.0 1.2044115185099984E-4 0.0 5.018381327124993E-4 0.0 26-27 0.0 1.2044115185099984E-4 0.0 8.02941012339999E-4 0.0 28-29 0.0 1.2044115185099984E-4 0.0 0.0013649997209779982 0.0 30-31 0.0 1.2044115185099984E-4 0.0 0.002790220017881496 0.0 32-33 0.0 1.2044115185099984E-4 0.0 0.004516543194412494 0.0 34-35 0.0 1.2044115185099984E-4 0.0 0.006824998604889991 0.0 36-37 0.0 1.2044115185099984E-4 0.0 0.009113380490058987 0.0 38-39 0.0 1.2044115185099984E-4 0.0 0.011160880071525985 0.0 40-41 0.0 1.2044115185099984E-4 0.0 0.014613526424587981 0.0 42-43 0.0 1.2044115185099984E-4 0.0 0.019471319549244977 0.0 44-45 2.0073525308499972E-5 1.2044115185099984E-4 0.0 0.026497053407219966 0.0 46-47 4.0147050616999945E-5 1.2044115185099984E-4 0.0 0.04155219738859495 0.0 48-49 4.0147050616999945E-5 1.2044115185099984E-4 0.0 0.0697956474976544 0.0 50-51 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.09221777526724888 0.0 52-53 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.11395740317635436 0.0 54-55 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.14210048565887132 0.0 56-57 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.16701173056671978 0.0 58-59 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.20067503250907423 0.0 60-61 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.2505778164260052 0.0 62-63 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.29355523411150364 0.0 64-65 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.33990500404883006 0.0 66-67 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.41178829817856843 0.0 68-69 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.49296563452614234 0.0 70-71 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.5684621632114107 0.0 72-73 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.6509242051787286 0.0 74-75 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.7308569829571756 0.0 76-77 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.8068954968257734 0.0 78-79 4.0147050616999945E-5 1.6058820246799978E-4 0.0 0.8939945231393548 0.0 80-81 4.0147050616999945E-5 1.6058820246799978E-4 0.0 1.0251147904544766 0.0 82-83 4.0147050616999945E-5 1.6058820246799978E-4 0.0 1.163682335659052 0.0 84-85 4.0147050616999945E-5 1.6058820246799978E-4 0.0 1.3073686298172948 0.0 86-87 4.0147050616999945E-5 1.6058820246799978E-4 0.0 1.513262778906579 0.0 88-89 4.0147050616999945E-5 1.6058820246799978E-4 0.0 1.7723919171140052 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15990 0.0 29.648909 1 GGTATCA 11115 0.0 28.321127 1 GTACATG 12550 0.0 26.257387 1 TACATGG 12750 0.0 25.501633 2 ATCAACG 19035 0.0 24.596832 3 TCAACGC 19130 0.0 24.502491 4 TATCAAC 19210 0.0 24.398977 2 CAACGCA 19205 0.0 24.356318 5 ACATGGG 12830 0.0 24.303734 3 AACGCAG 19575 0.0 23.908192 6 GAGTACT 9910 0.0 22.720882 12-13 TACCTGG 1965 0.0 21.288359 2 ACGCAGA 22100 0.0 21.1551 7 CGCAGAG 22195 0.0 20.978924 8 CATGGGA 7365 0.0 20.913202 4 AGTACTT 10615 0.0 20.719604 12-13 TCGCGCA 115 0.0037190202 20.657402 9 GTACTTT 10740 0.0 20.65496 14-15 AGAGTAC 17315 0.0 20.082502 10-11 CATGGGG 5265 0.0 19.864271 4 >>END_MODULE