FastQCFastQC Report
Thu 26 May 2016
SRR937638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490843
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145480.5840592923761152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120240.48272813661880737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82290.3303700795272926No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44930.18038069842218077No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.12280982783740284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122750.028.9166241
GCGTTAG856.395947E-427.951141
GTATCAA174050.027.8740561
GTACATG120350.024.9528081
TACATGG123300.023.657852
ACATGGG122450.022.9685133
ATCAACG213450.022.7025153
TCAACGC213950.022.649464
TATCAAC214650.022.5091972
CAACGCA216300.022.4033855
AGTACTT126800.021.98924612-13
AACGCAG221150.021.8638276
GAGTACT122800.021.81585312-13
GGACCGA4700.021.2220236
GTACTTT127550.020.92768514-15
AGAGTAC194700.020.49296610-11
CATGGGA71200.020.1510164
ACGCAGA246700.019.4851117
CGCAGAG245550.019.4603028
TACTTTT144000.019.2956214-15
Produced by FastQC (version 0.11.5)