FastQCFastQC Report
Thu 26 May 2016
SRR937637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401855
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118980.49536712249490494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90520.3768753734093024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60600.2523049892687111No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40740.16961889872619287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33760.1405580270249453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG804.4833033E-429.70084
GTATCAA155550.028.916461
GGTATCA109600.028.6323741
GTACATG125450.027.2509861
TACATGG128100.026.1594072
ACATGGG129550.024.4300843
TATCAAC188100.023.6523862
ATCAACG188200.023.6091383
TCAACGC188900.023.546814
CAACGCA190200.023.3368745
AACGCAG194700.022.8128596
GAGTACT98000.022.60930312-13
GTACCTG19450.022.2456841
CATGGGG52250.021.737014
GTACTTT104750.021.06169114-15
AGTACTT104600.020.97837612-13
ACGCAGA215350.020.5591477
CGCAGAG215750.020.4769948
AGAGTAC172150.020.1138710-11
CATGGGA74950.020.0990644
Produced by FastQC (version 0.11.5)