FastQCFastQC Report
Thu 26 May 2016
SRR937637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401855
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141960.5910431728809608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118200.4921196325340205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80370.33461636943112716No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46350.19297584575255375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.12311317710686116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA176800.028.007811
GGTATCA126150.027.4997861
GTACATG118600.024.2818411
TACATGG122300.023.1490212
TATCAAC213100.022.959722
ATCAACG212850.022.9197393
TCAACGC213150.022.865194
TAGGACG1252.2562208E-422.8010084
GAGTACT117100.022.67795812-13
CAACGCA215950.022.6347145
AACGCAG221250.022.1756136
ATACCGT1754.0551204E-621.7125346
ACATGGG123900.021.6615733
GTACTTT123000.021.47025914-15
AGTACTT120150.021.43011712-13
AGAGTAC187000.020.61559710-11
GGACCGA4850.020.5653516
AGGACCG5950.019.9588665
ACGCAGA246700.019.8920787
CGCAGAG247200.019.7941918
Produced by FastQC (version 0.11.5)